Purification of the medium-chain/long-chain (COT/CPT) carnitine acyltransferase of rat liver microsomes.

abstract：:Expression of recombinant proteins in bacterial or eukaryotic systems often results in aggregation rendering them unavailable for biochemical or structural studies. Protein aggregation is a costly problem for biomedical research. It forces research laboratories and the biomedical industry to search for alternative, mo...

journal_title：Protein expression and purification

authors： Pullara F,Guerrero-Santoro J,Calero M,Zhang Q,Peng Y,Spåhr H,Kornberg GL,Cusimano A,Stevenson HP,Santamaria-Suarez H,Reynolds SL,Brown IS,Monga SP,Van Houten B,Rapić-Otrin V,Calero G,Levine AS

更新日期：2013-02-01 00:00:00

abstract：:Leishmaniasis is considered by the World Health Organization to be the second most important disease caused by a protozoan parasite. Biochemical and molecular biology studies can help in the understanding of the biology of the Leishmania parasite. All protozoan parasites, including Leishmania, are unable to synthesize...

journal_title：Protein expression and purification

authors： de Oliveira AH,Ruiz JC,Cruz AK,Greene LJ,Rosa JC,Ward RJ

更新日期：2006-10-01 00:00:00

abstract：:Pokeweed antiviral protein (PAP)-I from the spring leaves of Phytolacca americana is a naturally occurring RNA-depurinating enzyme with broad-spectrum antiviral activity. Interest in PAP is growing due to its use as a potential anti-HIV agent. However, the clinical use of native PAP is limited due to inherent difficul...

journal_title：Protein expression and purification

authors： Rajamohan F,Doumbia SO,Engstrom CR,Pendergras SL,Maher DL,Uckun FM

更新日期：2000-03-01 00:00:00

abstract：:NAD(+)-dependent malic enzyme (NAD-ME) gene from Escherichia coli K12 was inserted into an expression vector pET24b(+) and transformed into E. coli BL21 (DE3). Recombinant NAD-ME was expressed upon IPTG induction, purified with affinity chromatography, and biochemically characterized. The results showed that recombina...

journal_title：Protein expression and purification

authors： Wang J,Tan H,Zhao ZK

更新日期：2007-05-01 00:00:00

abstract：:Hepatocyte growth factor (HGF) is a multifunctional protein expressed in a variety of cell types and tissues. Here we describe a novel one-step method to separate and identify HGF, based on a unique interaction between HGF and Cu(II). Conditioned medium (CM) from mouse 3T3-L1 adipocytes which contains HGF or purified ...

journal_title：Protein expression and purification

authors： Rahimi N,Etchells S,Elliott B

更新日期：1996-05-01 00:00:00

abstract：:Chan Su is a traditional medicine prepared from toxic secretions from the auricular and skin glands of Chinese toads. Previous studies show that active components in Chan Su can inhibit the proliferation of tumor cells. To study the effect of Chan Su peptides on angiogenesis, fresh Chan Su was collected and its compon...

journal_title：Protein expression and purification

authors： Xia F,Gao F,Yao H,Zhang G,Gao B,Lu Y,Wang X,Qian Y

更新日期：2019-11-01 00:00:00

abstract：:Endostatin, a carboxy-terminal fragment of collagen XVIII, has been shown to act as an anti-angiogenic agent that specifically inhibits proliferation of endothelial cells and growth of various primary tumors. Here, we describe the expression by Chinese hamster ovary (CHO) cells of murine endostatin and of a tagged-fus...

journal_title：Protein expression and purification

authors： Chura-Chambi RM,Tornieri PH,Spencer PJ,Nascimento PA,Mathor MB,Morganti L

更新日期：2004-05-01 00:00:00

abstract：:PDZ (PSD-95, DiscsLarge, ZO1) domains function in nature as protein binding domains within scaffold and membrane-associated proteins. They comprise ∼90 residues and make specific, high affinity interactions with complementary C-terminal peptide sequences, with other PDZ domains, and with phospholipids. We hypothesized...

journal_title：Protein expression and purification

authors： Walkup WG 4th,Kennedy MB

更新日期：2014-06-01 00:00:00

abstract：:Proteus mirabilis (P. mirabilis) is a zoonotic pathogen that has recently presented a rising infection rate in the poultry industry. To develop an effective vaccine to protect chickens against P. mirabilis infection, OmpA, one of the major outer membrane proteins of P. mirabilis, was expressed in Pichia pastoris. The ...

journal_title：Protein expression and purification

authors： Zhang Y,Yang S,Dai X,Liu L,Jiang X,Shao M,Chi S,Wang C,Yu C,Wei K,Zhu R

更新日期：2015-01-01 00:00:00

abstract：:Resistance to Inhibitors of Cholinesterase-8 (Ric-8) proteins are molecular chaperones that fold heterotrimeric G protein α subunits shortly after biosynthesis. Ric-8 proteins also act as test tube guanine nucleotide exchange factors (GEF) that promote Gα subunit GDP for GTP exchange. The GEF and chaperoning activitie...

journal_title：Protein expression and purification

authors： Yu W,Yu M,Papasergi-Scott MM,Tall GG

更新日期：2019-02-01 00:00:00

abstract：:Protein aggregation is a main barrier hindering structural and functional studies of a number of interesting biological targets. The E6 oncoprotein of Human Papillomavirus strain 16 (E6(16)) is difficult to express under a native soluble form in bacteria. Produced as an unfused sequence, it forms inclusion bodies. Fus...

journal_title：Protein expression and purification

authors： Zanier K,Nominé Y,Charbonnier S,Ruhlmann C,Schultz P,Schweizer J,Travé G

更新日期：2007-01-01 00:00:00

abstract：:The covalent addition of 4-amino-4-deoxy-L-arabinose (L-Ara4N) groups to lipid A, which resides in the outer membranes of bacteria such as Salmonella typhimurium and Escherichia coli, is the final step in the polymyxin-resistance pathway in these organisms. This modification is catalyzed by the inner membrane protein ...

journal_title：Protein expression and purification

authors： Bretscher LE,Morrell MT,Funk AL,Klug CS

更新日期：2006-03-01 00:00:00

abstract：:Hydrophobic charge-induction chromatography (HCIC) using 4-mercaptoethylpyridine (4-MEP) as the ligand is used to purify antibodies. The 4-MEP resin ligand has high affinity for antibodies, which makes it difficult to optimize the elution conditions. Recent studies showed that arginine is effective at eluting and puri...

journal_title：Protein expression and purification

authors： Hirano A,Maruyama T,Shiraki K,Arakawa T,Kameda T

更新日期：2017-01-01 00:00:00

abstract：:Borneol is a bicyclic plant monoterpene. It can be degraded by soil microorganisms through the conversion of borneol dehydrogenase (BDH) and a known camphor degradation pathway. Recombinant BDH from Pseudomonas sp. TCU-HL1 was produced in the form of inclusion body. The refolded BDH1 tends to precipitate. Insoluble re...

journal_title：Protein expression and purification

authors： Khine AA,Lu PC,Ko TP,Huang KF,Chen HP

更新日期：2020-11-01 00:00:00

abstract：:While well established in bacterial hosts, the effect of coding sequence variation on protein expression in mammalian systems is poorly characterized outside of viral proteins or proteins from distant phylogenetic families. The potential impact is substantial given the extensive use of mammalian expression systems in ...

journal_title：Protein expression and purification

authors： Carton JM,Sauerwald T,Hawley-Nelson P,Morse B,Peffer N,Beck H,Lu J,Cotty A,Amegadzie B,Sweet R

更新日期：2007-10-01 00:00:00

abstract：:Enteropeptidase (synonym: enterokinase, EC 3.4.21.9) is a heterodimeric serine protease of the intestinal brush border that activates trypsinogen by highly specific cleavage of the trypsinogen activation peptide following the sequence (Asp)(4)-Lys. It has also great biotechnological interest because of the unique subs...

journal_title：Protein expression and purification

authors： Gasparian ME,Bychkov ML,Dolgikh DA,Kirpichnikov MP

更新日期：2011-10-01 00:00:00

abstract：:The gene encoding S. cerevisiae Kex2 protease derivative Kex2-667 (encoding the N-terminal 20th to 667th amino acid residues of Kex2 protease, containing the propeptide, catalytic domain, P domain and Ser/Thr enrichment region) and its 225th amino acid residue mutant K225L were overexpressed in Pichia pastoris. Protea...

journal_title：Protein expression and purification

authors： Yang F,Liu L,Liu Y,Li S

更新日期：2020-12-01 00:00:00

abstract：:The Gradiflow, a preparative electrophoresis instrument designed to separate molecules on the basis of their size and charge, was used to purify antibody Fab and F(ab')2 fragments. The method described is charge based, utilizing the difference in the pI between the antibody Fab/F(ab')2 fragments and antibody Fc fragme...

journal_title：Protein expression and purification

authors： Cheung GL,Thomas TM,Rylatt DB

更新日期：2003-11-01 00:00:00

abstract：:The ELR-CXC chemokines are important to neutrophil inflammation in many acute and chronic diseases. Among them, CXCL8 (interleukin-8, IL-8), binds to both the CXCR1 and CXCR2 receptors with high affinity and the expression levels of CXCL8 are elevated in many inflammatory diseases. Recently, an analogue of human CXCL8...

journal_title：Protein expression and purification

authors： Cheng HT,Huang KC,Yu HY,Gao KJ,Zhao X,Li F,Town J,Gordon JR,Cheng JW

更新日期：2008-09-01 00:00:00

abstract：:This article reviews the development of immobilized-metal affinity chromatography (IMAC) and describes its most important applications. We provide an overview on the use of IMAC in protein fractionation and proteomics, in protein immobilization and detection, and on some special applications such as purification of im...

journal_title：Protein expression and purification

authors： Block H,Maertens B,Spriestersbach A,Brinker N,Kubicek J,Fabis R,Labahn J,Schäfer F

更新日期：2011-09-03 00:00:00

abstract：:A functional IP10-scFv fusion protein retaining the antibody specificity for acidic isoferritin and chemokine function was produced at high level in Esherichia coli (E. coli). IP10-scFv gene from the recombinant plasmid pc3IP104c9 was subcloned into pET28a fused to N-terminal His-tag sequence in frame and overexpresse...

journal_title：Protein expression and purification

authors： Guo JQ,Li QM,Zhou JY,Zhang GP,Yang YY,Xing GX,Zhao D,You SY,Zhang CY

更新日期：2006-01-01 00:00:00

abstract：:Hendra virus (family Paramyxoviridae) is a negative sense single-stranded RNA virus (NSRV) which has been found to cause disease in humans, horses, and experimentally in other animals, e.g. pigs and cats. Pteropid bats commonly known as flying foxes have been identified as the natural host reservoir. The Hendra virus ...

journal_title：Protein expression and purification

authors： Pearce LA,Yu M,Waddington LJ,Barr JA,Scoble JA,Crameri GS,McKinstry WJ

更新日期：2015-12-01 00:00:00

abstract：:Separation of copurifying protease activity from recombinant human Müllerian inhibiting substance (rhMIS) bound to a monoclonal antibody immunoaffinity column by a high-salt wash results in cleaner preparations of rhMIS resistant to cleavage upon storage. In addition, an inhibitor of rhMIS antiproliferative activity i...

journal_title：Protein expression and purification

authors： Ragin RC,Donahoe PK,Kenneally MK,Ahmad MF,MacLaughlin DT

更新日期：1992-06-01 00:00:00

abstract：:Mitochondrial 2,4-dienoyl-CoA reductase is a key enzyme for the beta-oxidation of unsaturated fatty acids. The cDNA of the full-length human mitochondrial 2,4-dienoyl-CoA reductase was previously cloned as pUC18::DECR. PCR methodologies were used to subclone the genes encoding various truncated human mitochondrial 2,4...

journal_title：Protein expression and purification

authors： Chu X,Yu W,Chen G,Li D

更新日期：2003-10-01 00:00:00

abstract：:Recombinant barley high pI alpha-glucosidase was produced by high cell-density fermentation of Pichia pastoris expressing the cloned full-length gene. The gene was amplified from a genomic clone and exons (coding regions) were assembled by overlap PCR. The resulting cDNA was expressed under control of the alcohol oxid...

journal_title：Protein expression and purification

authors： Naested H,Kramhøft B,Lok F,Bojsen K,Yu S,Svensson B

更新日期：2006-03-01 00:00:00

abstract：:Human extracellular superoxide dismutase (hEC-SOD) is an enzyme that scavenges reactive oxygen species (ROS). Because of its antioxidant activity, hEC-SOD has been used as a therapeutic protein to treat skin disease and arthritis in mammalian systems. In this study, codon-optimized hEC-SOD was expressed in tobacco (Ni...

journal_title：Protein expression and purification

authors： Park KY,Kim EY,Lee W,Kim TY,Kim WT

更新日期：2016-03-01 00:00:00

abstract：:The expression of recombinant proteins in bacterial hosts may alter the biophysical properties of the protein of interest as a result of differences in post-translational processing from that observed when produced in the native cell. For example, recombinant human interleukin-1beta (IL-1beta) is produced as three iso...

journal_title：Protein expression and purification

authors： Covalt JC Jr,Cao TB,Magdaroag JR,Gross LA,Jennings PA

更新日期：2005-05-01 00:00:00

abstract：:The HIV-1 Gag precursor protein, Pr55(Gag), is a multi-domain polyprotein that drives HIV-1 assembly. The morphological features of HIV-1 suggested Pr55(Gag) assumes a variety of different conformations during virion assembly and maturation, yet structural determination of HIV-1 Pr55(Gag) has not been possible due to ...

journal_title：Protein expression and purification

authors： McKinstry WJ,Hijnen M,Tanwar HS,Sparrow LG,Nagarajan S,Pham ST,Mak J

更新日期：2014-08-01 00:00:00

abstract：:Periplasmic expression of recombinant proteins ensures the production of biologically active proteins in a correctly folded state with several key advantages. This research focused on the in-frame cloning of rhIL-15 in pET-20 (+) vector with pelB-leader sequence to direct the protein to the bacterial periplasm. The ta...

journal_title：Protein expression and purification

authors： Ahmed N,Afroze B,Abbas R,Khan MA,Akram M,Tahir S,Bakht S,Munir A,Shahid AA

更新日期：2021-01-01 00:00:00

abstract：:Aminopeptidase B (Ap-B) is a ubiquitous enzyme and its physiological function still remains an open question. This Zn2+ -exopeptidase catalyzes the amino-terminal cleavage of basic residues of peptide or protein substrates, indicating a role in precursor processing. In addition, the enzyme exhibits a residual capacity...

journal_title：Protein expression and purification

authors： Cadel S,Gouzy-Darmon C,Petres S,Piesse C,Pham VL,Beinfeld MC,Cohen P,Foulon T

更新日期：2004-07-01 00:00:00