Human müllerian inhibiting substance: enhanced purification imparts biochemical stability and restores antiproliferative effects.

abstract：:The recombinant truncated endolysin LysK consisting of two catalytic domains, N-terminal CHAP and amidase-2 (LysKCA) was overexpressed in E. coli in the form of inclusion bodies (IBs). These IBs were dissolved in 6 M solution of urea followed by the refolding process. The refolding efficacy of the dilution and matrix-...

journal_title：Protein expression and purification

authors： Alaksandr Ž,Sergey G,Maksim P,Sergey K,Niyaz S,Uladzimir P,Mikhail S

更新日期：2020-10-01 00:00:00

abstract：:A new procedure for the large-scale purification of the recombinant thermostable chitinase (Chi40) cloned from Streptomyces thermoviolaceus in various expression vectors in Escherichia coli is described. Chi40 was overproduced in the cytosolic and secreted forms. The cytosolic form (Chi40c) was highly overproduced and...

journal_title：Protein expression and purification

authors： Christodoulou E,Duffner F,Vorgias CE

更新日期：2001-10-01 00:00:00

abstract：:There is no high-resolution structure for the membrane domain of the human erythrocyte anion exchanger, AE1 (Band 3). In this report, we have developed an expression and purification strategy for AE1 to be used in crystallization trials. Saccharomyces cerevisiae strain BJ5457 was transformed with an expression vector ...

journal_title：Protein expression and purification

authors： Bonar P,Casey JR

更新日期：2010-11-01 00:00:00

abstract：:Proteomics has become a major focus as researchers attempt to understand the vast amount of genomic information. Protein complexity makes identifying and understanding gene function inherently difficult. The challenge of studying proteins in a global way is driving the development of new technologies for systematic an...

journal_title：Protein expression and purification

authors： Lesley SA

更新日期：2001-07-01 00:00:00

abstract：:Human granulocyte-macrophage colony-stimulating factor (GM-CSF), a hemopoietic growth factor, was produced and secreted from tobacco cell suspensions. The GM-CSF cDNA was carried by a binary vector under the control of the CaMV 35S promoter and the T7 terminator. In addition, a 5'-nontranslated region from the tobacco...

journal_title：Protein expression and purification

authors： James EA,Wang C,Wang Z,Reeves R,Shin JH,Magnuson NS,Lee JM

更新日期：2000-06-01 00:00:00

abstract：:3-Deoxy-d-manno-octulosonate 8-phosphate synthase (KDO8PS) [EC 4.1.2.16] is the first and rate-limiting enzyme in the 3-deoxy-d-manno-octulosonate (KDO) biosynthetic pathway. The enzyme is widely expressed in bacteria and plants. Their well conserved protein sequences imply a similar oligomeric arrangement. However, t...

journal_title：Protein expression and purification

authors： Zhang F,Xu Y,Zhang Z

更新日期：2014-09-01 00:00:00

abstract：:The XRCC1 DNA repair protein contains two regions of approximately 100 amino acids each that share homology with the BRCT (BRCA1 carboxyl terminus) domain superfamily. These two regions of XRCC1 have been shown to interact independently with DNA ligase III and poly(ADP-ribose)polymerase as part of a mechanism involved...

journal_title：Protein expression and purification

authors： Thornton K,Forstner M,Shen MR,West MG,Rupp B,Thelen MP

更新日期：1999-07-01 00:00:00

abstract：:A gene encoding a glutamate-specific endopeptidase (GSE) from Bacillus licheniformis (BL) has been cloned in Escherichia coli cells. The recombinant protein was expressed as cytoplasmic insoluble inclusion bodies. Immobilized metal affinity chromatography was employed to purify the protein, and then a 27-kDa GSE inter...

journal_title：Protein expression and purification

authors： Ye W,Liu J,Wang H,Wang J,Wang X

更新日期：2012-03-01 00:00:00

abstract：:The aim of this study was to introduce a simple, reproducible, and less expensive method for isolation of alpha-lactalbumin, beta-lactoglobulin, and bovine serum albumin from cow's milk while retaining their antigenicity. Whey (lactoserum) was obtained by isolating casein from defatted milk using hydrochloric acid. Gl...

journal_title：Protein expression and purification

authors： Neyestani TR,Djalali M,Pezeshki M

更新日期：2003-06-01 00:00:00

abstract：:Ciliary neurotrophic factor (CNTF) is a promising candidate for the treatment of neurodegenerative or metabolic diseases, but suffers rapid clearance in body. Herein we constructed a new long-acting recombinant human CNTF (rhCNTF) by genetic fusion with an albumin-binding domain (ABD) through a flexible peptide linker...

journal_title：Protein expression and purification

authors： Xu L,Zhang C,Liu L,Zhang Y,Wang Q,Wang J,Liu Y,Su Z

更新日期：2017-11-01 00:00:00

abstract：:Pseudolysin is the extracellular elastase of Pseudomonas aeruginosa and belongs to the thermolysin-like family of metallopeptidases. Pseudolysin has been identified as a robust drug target and a biotechnologically important enzyme in the tanning industry. Previous attempts to purify active pseudolysin from P. aerugino...

journal_title：Protein expression and purification

authors： Odunuga OO,Adekoya OA,Sylte I

更新日期：2015-09-01 00:00:00

abstract：:Nedd4 is an E3 ubiquitin ligase that has received increased attention due to its role in the maintenance of proteostasis and in cellular stress responses. Investigation of Nedd4 enzymology has revealed a complex enzymatic mechanism that involves intermolecular interactions with upstream E2 conjugating enzymes and with...

journal_title：Protein expression and purification

authors： Hatstat AK,McCafferty DG

更新日期：2020-09-01 00:00:00

abstract：:The purpose of this work was to characterize the functions of two sesquiterpene synthase genes from moso bamboo (Phyllostachys edulis). Two novel sesquiterpene synthase genes, belonging to the Tpsa subfamily, were isolated from moso bamboo. MoTPS2 was 1641 bp in length and encoded a protein of 63 kDa, whereas MoTPS6 w...

journal_title：Protein expression and purification

authors： Chen X,Wang Y,Sun J,Wang J,Xun H,Tang F

更新日期：2016-04-01 00:00:00

abstract：:The HIV-1 Gag precursor protein, Pr55(Gag), is a multi-domain polyprotein that drives HIV-1 assembly. The morphological features of HIV-1 suggested Pr55(Gag) assumes a variety of different conformations during virion assembly and maturation, yet structural determination of HIV-1 Pr55(Gag) has not been possible due to ...

journal_title：Protein expression and purification

authors： McKinstry WJ,Hijnen M,Tanwar HS,Sparrow LG,Nagarajan S,Pham ST,Mak J

更新日期：2014-08-01 00:00:00

abstract：:A method is presented to produce large amounts of Bcl-2 and Bcl-x(L), two anti-apoptotic proteins of considerable biomedical interest. Expression constructs were prepared in which the Escherichia coli protein TolAIII, known to promote over expression of soluble product, was added to the N-terminus of Bcl-2 or Bcl-x(L)...

journal_title：Protein expression and purification

authors： Nedelkina S,Gokce I,Ridley H,Weckerle C,Magnin T,Vallette F,Pattus F,Lakey JH,Bechinger B

更新日期：2008-08-01 00:00:00

abstract：:A putative laccase gene (lacG) from Geobacillus sp. JS12 was cloned and expressed as a fusion protein with six histidine residues in Escherichia coli BL21 (DE3) cells, and the protein was primarily found in inclusion bodies. The resulting insoluble proteins were solubilized with 6 M guanidine HCl and refolded using an...

journal_title：Protein expression and purification

authors： Jeon SJ,Park JH

更新日期：2020-09-01 00:00:00

abstract：:Soluble guanylate cyclase (sGC) has been purified from 100 L cell culture infected by baculovirus using the newer and highly effective titerless infected-cells preservation and scale-up (TIPS) method. Successive passage of the enzyme through DEAE, Ni(2+)-NTA, and POROS Q columns obtained approximately 100mg of protein...

journal_title：Protein expression and purification

authors： Emmons TL,Mathis KJ,Shuck ME,Reitz BA,Curran DF,Walker MC,Leone JW,Day JE,Bienkowski MJ,Fischer HD,Tomasselli AG

更新日期：2009-06-01 00:00:00

abstract：:Rotavirus VP8* subunit is the minor trypsin cleavage product of the spike protein VP4, which is the major determinant of the viral infectivity and neutralization. To study the structure-function relationship of this fragment and to obtain type-specific reagents, substantial amounts of this protein are needed. Thus, fu...

journal_title：Protein expression and purification

authors： Favacho AR,Kurtenbach E,Sardi SI,Gouvea VS

更新日期：2006-04-01 00:00:00

abstract：:Dengue virus causes serious diseases affecting people in tropical and sub-tropical regions. The nonstructural (NS) protein 2B is an integral membrane protein and important for the regulation of viral protease NS3, which is significant for virus replication. The NS2B-NS3 complex is an important drug target for treating...

journal_title：Protein expression and purification

authors： Huang Q,Chen AS,Li Q,Kang C

更新日期：2011-12-01 00:00:00

abstract：:Cyclophilins are highly conserved proteins associated with peptidyl-prolyl cis-trans isomerase activity (PPIase). The present study was designed to analyze the biological activity of recombinant cyclophilin from the marine red algae Pyropia yezoensis (PyCyp). The cyclophilin gene from P. yezoensis was cloned into the ...

journal_title：Protein expression and purification

authors： Ulagesan S,Choi JW,Nam TJ,Choi YH

更新日期：2020-08-01 00:00:00

abstract：:Hydrophobic interaction high-performance liquid chromatography (HIC-HPLC) was utilized for the separation of native human antithrombin (AT) and a partially denaturated form of AT, known as the latent form (L-AT). The AT used in this study is commercially available (Atenativ, Pharmacia & Upjohn, Sweden) and contains al...

journal_title：Protein expression and purification

authors： Karlsson G,Winge S

更新日期：2001-02-01 00:00:00

abstract：:High affinity interaction between S-protein and S-peptide fragments of bovine pancreatic RNase A has been recently used for construction of molecular vehicles for targeted drug delivery. The vehicle is assembled as a complex of drug carrier conjugated S-protein with S-peptide-tagged targeting protein. To avoid random ...

journal_title：Protein expression and purification

authors： Backer MV,Gaynutdinov TI,Aloise R,Przekop K,Backer JM

更新日期：2002-12-01 00:00:00

abstract：:A high-expression plasmid, pKSGHE3-1, containing the salt-tolerant glutaminase (EC 3.5.1.2) from marine bacterium Micrococcus luteus K-3 was constructed. pKSGHE3-1 was made by inserting the DNA fragment (1.43 kb) containing the structural gene synthesized by polymerase chain reaction into the downstream region of the ...

journal_title：Protein expression and purification

authors： Nandakumar R,Wakayama M,Nagano Y,Kawamura T,Sakai K,Moriguchi M

更新日期：1999-03-01 00:00:00

abstract：:Our earlier studies have demonstrated that the 35 kDa isoform of Translocated promoter region protein (Tpr) of Rattus norvegicus was able to augment c-jun transcription efficiently. Identification of direct targets that may in part downregulate c-jun transcription might prove to be an ideal target to curtail the proli...

journal_title：Protein expression and purification

authors： Agarwal S,Yadav SK,Dixit A

更新日期：2011-05-01 00:00:00

abstract：:Lactobacillus β-galactosidases are mostly heterodimeric proteins, which are encoded by the two overlapping genes, lacL and lacM, and produced in recombinant prokaryotic systems for higher yield. This is the first report on the expression of a heterodimeric β-galactosidase from Lactobacillus crispatus B470 in Pichia pa...

journal_title：Protein expression and purification

authors： Nie C,Liu B,Zhang Y,Zhao G,Fan X,Ning X,Zhang W

更新日期：2013-11-01 00:00:00

abstract：:The gene that was inferred to encode the TyrR protein of Haemophilus influenzae Rd was synthesized by polymerase chain reaction and inserted into a T7-based expression vector. Methods were developed to overexpress the TyrR protein of H. influenzae in Escherichia coli and to purify the protein on a large scale. Both in...

journal_title：Protein expression and purification

authors： Zhu Q,Zhao S,Somerville RL

更新日期：1997-07-01 00:00:00

abstract：:The full-length human acetyl-CoA carboxylase 1 (ACC1) was expressed and purified to homogeneity by two separate groups (Y.G. Gu, M. Weitzberg, R.F. Clark, X. Xu, Q. Li, T. Zhang, T.M. Hansen, G. Liu, Z. Xin, X. Wang, T. McNally, H. Camp, B.A. Beutel, H.I. Sham, Synthesis and structure-activity relationships of N-{3-[2...

journal_title：Protein expression and purification

authors： Kim KW,Yamane H,Zondlo J,Busby J,Wang M

更新日期：2007-05-01 00:00:00

abstract：:Human extracellular superoxide dismutase (hEC-SOD) is an enzyme that scavenges reactive oxygen species (ROS). Because of its antioxidant activity, hEC-SOD has been used as a therapeutic protein to treat skin disease and arthritis in mammalian systems. In this study, codon-optimized hEC-SOD was expressed in tobacco (Ni...

journal_title：Protein expression and purification

authors： Park KY,Kim EY,Lee W,Kim TY,Kim WT

更新日期：2016-03-01 00:00:00

abstract：:Recombinant barley high pI alpha-glucosidase was produced by high cell-density fermentation of Pichia pastoris expressing the cloned full-length gene. The gene was amplified from a genomic clone and exons (coding regions) were assembled by overlap PCR. The resulting cDNA was expressed under control of the alcohol oxid...

journal_title：Protein expression and purification

authors： Naested H,Kramhøft B,Lok F,Bojsen K,Yu S,Svensson B

更新日期：2006-03-01 00:00:00

abstract：:Characterizing protein complexes and identifying their subunits promote our understanding of the machinery involved in many in vivo processes. Proteomic studies can identify a protein's binding partners, and this can provide insight into how protein complexes function and how they are regulated. In addition, the compo...

journal_title：Protein expression and purification

authors： Banks CA,Kong SE,Washburn MP

更新日期：2012-12-01 00:00:00