Isolation of alpha-lactalbumin, beta-lactoglobulin, and bovine serum albumin from cow's milk using gel filtration and anion-exchange chromatography including evaluation of their antigenicity.

abstract：:Bovine pancreatic procarboxypeptidase A has been overexpressed in a soluble and activatable form in Escherichia coli. When the protein was expressed under the control of bacteriophage T7 promoter in E. coli ADA494 (a thioredoxin reductase deficient bacteria), a thioredoxin fusion protein was produced at relatively hig...

journal_title：Protein expression and purification

authors： Seddi R,Chaix JC,Puigserver A,Guo XJ

更新日期：2003-02-01 00:00:00

abstract：:The guanine nucleotide exchange factor EF-1beta gene from the thermoacidophilic archaeon Sulfolobus solfataricus (SsEF-1beta) was amplified by PCR and cloned into the pT7-7 expression vector. One of four selected clones harbored the T160C nucleotide substitution leading to the Y54H amino acid change in a hydrophobic r...

journal_title：Protein expression and purification

authors： Ianniciello G,Masullo M,Raimo G,Arcari P,Bocchini V

更新日期：1998-02-01 00:00:00

abstract：:The complement regulatory protein (CRP) of Trypanosoma cruzi is a developmentally regulated glycosylphosphatidylinositol (GPI)-anchored membrane protein that protects the parasite from complement-mediated killing, and is an important virulence determinant of the microorganism. CRP binds human complement components C3b...

journal_title：Protein expression and purification

authors： Beucher M,Meira WS,Zegarra V,Galvão LM,Chiari E,Norris KA

更新日期：2003-01-01 00:00:00

abstract：:κ-Carrageenase (EC3.2.1.83) is a class of glycoside hydrolase, which can be used for hydrolysis of κ-carrageenan to κ-carrageenan oligosaccharides. In this study, a bacterium, identified as Pseudoalteromonas sp. ZDY3 isolated from rotten algae, was capable to degrade κ-carrageenan. The κ-carrageenase produced by Pseud...

journal_title：Protein expression and purification

authors： Zhao D,Jiang B,Zhang Y,Sun W,Pu Z,Bao Y

更新日期：2021-02-01 00:00:00

abstract：:The P(II) proteins comprise a family of widely distributed signal transduction proteins that integrate the signals of cellular nitrogen, carbon and energy status, and then regulate, by protein-protein interaction, the activity of a variety of target proteins including enzymes, transcriptional regulators and membrane t...

journal_title：Protein expression and purification

authors： Moure VR,Razzera G,Araújo LM,Oliveira MAS,Gerhardt ECM,Müller-Santos M,Almeida F,Pedrosa FO,Valente AP,Souza EM,Huergo LF

更新日期：2012-01-01 00:00:00

abstract：:The purpose of this work was to characterize the functions of two sesquiterpene synthase genes from moso bamboo (Phyllostachys edulis). Two novel sesquiterpene synthase genes, belonging to the Tpsa subfamily, were isolated from moso bamboo. MoTPS2 was 1641 bp in length and encoded a protein of 63 kDa, whereas MoTPS6 w...

journal_title：Protein expression and purification

authors： Chen X,Wang Y,Sun J,Wang J,Xun H,Tang F

更新日期：2016-04-01 00:00:00

abstract：:Soybean root nodules possess a developmentally regulated acid phosphatase (ACP) that exhibits the highest specificity for purine 5'-nucleoside monophosphates. The enzyme is a glycosylated dimer of 28- and 31-kDa subunits, which appear to be products of the same gene but differ in posttranslational modifications. In or...

journal_title：Protein expression and purification

authors： Penheiter AR,Klucas RV,Sarath G

更新日期：1998-10-01 00:00:00

abstract：:The catalase gene of Psychrobacter sp. T-3 was cloned, and the gene product (PktA) was overexpressed in Escherichia coli. The specific activity of the purified PktA was slightly lower than that of the native purified enzyme obtained from Psychrobacter sp. T-3. Spectrophotometric measurements of the purified enzymes su...

journal_title：Protein expression and purification

authors： Kimoto H,Matsuyama H,Yumoto I,Yoshimune K

更新日期：2008-06-01 00:00:00

abstract：:Escherichia coli cells were transformed with an expression vector constructed by inserting a DNA fragment encoding a Kazal-type trypsin inhibitor from mouse seminal vesicle into pGEX-2. The cloned cells were able to produce a high yield of a chimeric polypeptide made by fusing the trypsin inhibitor to glutathione S-tr...

journal_title：Protein expression and purification

authors： Lai ML,Li SH,Chen YH

更新日期：1994-02-01 00:00:00

abstract：:Recombinant murine MRP14 (mMRP14) was produced in Escherichia coli using the pGEX expression system. The mass of fusion protein, by electrospray ionization-mass spectrometry (ESI/MS), was 39,213 Da which compares well with the theoretical mass (39,210.4 Da). Thrombin digestion of fusion protein was expected at a clone...

journal_title：Protein expression and purification

authors： Raftery MJ,Collinson L,Geczy CL

更新日期：1999-03-01 00:00:00

abstract：:A TRAIL-CM4 fusion protein in soluble form with tumor selective apoptosis and antibacterial functions was expressed in the Escherichia coli expression system and isolated through dialysis refolding and histidine-tag Nickel-affinity purification. Fresh Jurkat cells were treated with the TRAIL-CM4 fusion protein. Trypan...

journal_title：Protein expression and purification

authors： Sang M,Zhang J,Li B,Chen Y

更新日期：2016-06-01 00:00:00

abstract：:Leishmaniasis is considered by the World Health Organization to be the second most important disease caused by a protozoan parasite. Biochemical and molecular biology studies can help in the understanding of the biology of the Leishmania parasite. All protozoan parasites, including Leishmania, are unable to synthesize...

journal_title：Protein expression and purification

authors： de Oliveira AH,Ruiz JC,Cruz AK,Greene LJ,Rosa JC,Ward RJ

更新日期：2006-10-01 00:00:00

abstract：:Ndt80 is a Saccharomyces cerevisiae meiosis-specific transcription factor responsible for promoting the stage-specific expression of a family of genes referred to as middle sporulation genes. Many members of this gene family are essential for the completion of meiotic chromosome segregation. Thus, Ndt80 is essential f...

journal_title：Protein expression and purification

authors： Sopko R,Stuart DT

更新日期：2004-01-01 00:00:00

abstract：:Acyl-acyl carrier protein synthase (Aas) is widely used to synthesize thioester adducts of fatty acids between 8 and 18 carbons in length enzymatically to the phosphopantetheine group of acyl carrier protein. The enzyme is an 80.6-kDa inner membrane protein that functions in vivo as a 2-acylglycerophosphoethanolamine ...

journal_title：Protein expression and purification

authors： Shanklin J

更新日期：2000-04-01 00:00:00

abstract：:Mammalian α-defensins contribute to innate immunity by exerting antimicrobial activity against various pathogens. To perform structural and functional analysis of α-defensins, large amounts of α-defensins are essential. Although many expression systems for the production of recombinant α-defensins have been developed,...

journal_title：Protein expression and purification

authors： Tomisawa S,Sato Y,Kamiya M,Kumaki Y,Kikukawa T,Kawano K,Demura M,Nakamura K,Ayabe T,Aizawa T

更新日期：2015-08-01 00:00:00

abstract：:Targeted anti-tumor toxins consist of a toxic functional moiety that is chemically linked or recombinantly fused to a cell-directing ligand. Ribosome-inactivating proteins (RIPs), especially type I RIPs such as saporin or dianthin, are commonly used as toxin components. Although expression of type I RIP-based fusion p...

journal_title：Protein expression and purification

authors： Gilabert-Oriol R,Thakur M,Weise C,Dernedde J,von Mallinckrodt B,Fuchs H,Weng A

更新日期：2013-09-01 00:00:00

abstract：:Darbepoetin alfa is an engineered and hyperglycosylated analog of recombinant human erythropoietin (EPO) which is used as a drug in treating anemia in patients with chronic kidney failure and cancer. This study desribes the secretory expression of a codon-optimized recombinant form of darbepoetin alfa in Leishmania ta...

journal_title：Protein expression and purification

authors： Kianmehr A,Golavar R,Rouintan M,Mahrooz A,Fard-Esfahani P,Oladnabi M,Khajeniazi S,Mostafavi SS,Omidinia E

更新日期：2016-02-01 00:00:00

abstract：:During the screening for tyrosine phenol-lyase-producing thermophiles, we isolated an obligatory symbiotic thermophile, Symbiobacterium sp. SC-1, which grew only in coculture with Bacillus sp. SK-1. A gene encoding thermostable tyrosine phenol-lyase (TPL) was cloned from the genomic DNA of the Symbiobacterium sp. SC-1...

journal_title：Protein expression and purification

authors： Lee SG,Hong SP,Choi YH,Chung YJ,Sung MH

更新日期：1997-12-01 00:00:00

abstract：:NAD(+)-dependent malic enzyme (NAD-ME) gene from Escherichia coli K12 was inserted into an expression vector pET24b(+) and transformed into E. coli BL21 (DE3). Recombinant NAD-ME was expressed upon IPTG induction, purified with affinity chromatography, and biochemically characterized. The results showed that recombina...

journal_title：Protein expression and purification

authors： Wang J,Tan H,Zhao ZK

更新日期：2007-05-01 00:00:00

abstract：:Stem cell factor (SCF) known as the c-kit ligand is a two disulfide bridge-containing cytokine in the regulation of the development and function of hematopoietic cell lineages and other cells such as mast cells, germ cells, and melanocytes. The secreted soluble form of SCF exists as noncovalently associated homodimer ...

journal_title：Protein expression and purification

authors： Akuta T,Kikuchi-Ueda T,Imaizumi K,Oshikane H,Nakaki T,Okada Y,Sultana S,Kobayashi K,Kiyokawa N,Ono Y

更新日期：2015-01-01 00:00:00

abstract：:Wild-type and deglycosylated forms of human prostate-specific antigen were expressed in Chinese hamster ovary (CHO) cells as zymogens. ProPSA was collected from conditioned medium and purified using a single cation-exchange chromatographic step for the deglycosylated form and cation-exchange followed by gel filtration...

journal_title：Protein expression and purification

authors： Bowman KK,Clark J,Yu L,Mortara K,Radika K,Wang J,Zhan H

更新日期：2000-12-01 00:00:00

abstract：:CD23, a 45-kDa type II membrane glycoprotein present on B cells, monocytes, and other human immune cells, is a low-affinity receptor for IgE. The extracellular region of the membrane-bound human CD23 is processed into at least four soluble (s) CD23 forms, with apparent molecular masses of 37, 33, 29, and 25 kDa. High ...

journal_title：Protein expression and purification

authors： Khandekar SS,Mayer RJ,Cusimano DM,Katchur SR,Appelbaum ER

更新日期：2001-07-01 00:00:00

abstract：:To expand the application of the streptavidin-biotin technology for reversible affinity purification of biotinylated proteins, a novel form of monomeric streptavidin was engineered and produced using Bacillus subtilis as the expression host. By changing as little as two amino acid residues (T90 and D128) to alanine, t...

journal_title：Protein expression and purification

authors： Qureshi MH,Wong SL

更新日期：2002-08-01 00:00:00

abstract：:Acyl carrier protein (ACP) was purified from Euglena gracilis variety bacillaris in yields of about 1 mg/100 g (wet wt) of cells. Antibodies against the purified protein were raised in hens and isolated from eggs. Antibodies raised against Euglena ACP inhibited the Euglena chloroplast nonaggregated fatty acid syntheta...

journal_title：Protein expression and purification

authors： Williams SG,Worsham LM,Ernst-Fonberg ML

更新日期：1991-04-01 00:00:00

abstract：:The human cyclin H, a protein normally associated with the cyclin-dependent kinase cdk7, was overexpressed in Escherichia coli using a T7 RNA polymerase expression system and further purified to apparent homogeneity. The purified recombinant cyclin H is similar to the endogenous one according to the following criteria...

journal_title：Protein expression and purification

authors： Poterszman A,Andersen G,Busso D,Rossignol M,Egly JM,Thierry JC

更新日期：1997-03-01 00:00:00

abstract：:The fluorescent reporter enhanced Green Fluorescent Protein (EGFP) has been used for assaying a wide range of biological activities ranging from gene expression, or localization of target proteins through to intermolecular interactions. However, over-production of this protein in Escherichia coli has resulted in the p...

journal_title：Protein expression and purification

authors： Youell J,Fordham D,Firman K

更新日期：2011-10-01 00:00:00

abstract：:Optimization of coding sequences to maximize protein expression yield is often outsourced to external service providers during commercial gene synthesis and thus unfortunately remains a black box for many researchers. The presented software program "CodonWizard" offers scientists a powerful but easy-to-use tool for cu...

journal_title：Protein expression and purification

authors： Rehbein P,Berz J,Kreisel P,Schwalbe H

更新日期：2019-08-01 00:00:00

abstract：:Molecular-level investigation of proteins is increasingly important to researchers trying to understand the mechanisms of signal transmission. Heterotrimeric G proteins control the activation of many critical signal transmission cascades and are also implicated in numerous diseases. As part of a longer-term investigat...

journal_title：Protein expression and purification

authors： Maly J,Crowhurst KA

更新日期：2012-08-01 00:00:00

abstract：:The availability of recombinant monomeric alkaline phosphatase (AP) is highly desirable in analytical applications involving AP fusion proteins. The cobalt-dependant alkaline phosphatase IV from Bacillus subtilis (BSAP), which was reported to be strongly monomeric, was overexpressed in Escherichia coli using pET autoi...

journal_title：Protein expression and purification

authors： Koksharov M,Lv C,Zhai X,Ugarova N,Huang E

更新日期：2013-08-01 00:00:00

abstract：:The Human Secretome Project aims to produce and purify all human secreted proteins as full-length. In order to enable this, a robust, gentle and effective purification process is needed, where multiple proteins can be purified in parallel. For this reason, a purification system based on a Protein C-tag and the HPC4 an...

journal_title：Protein expression and purification

authors： Kanje S,Enstedt H,Dannemeyer M,Uhlén M,Hober S,Tegel H

更新日期：2020-11-01 00:00:00