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Production and single-step purification of EGFP and a biotinylated version of the Human Rhinovirus 14 3C protease.

Abstract：

:The fluorescent reporter enhanced Green Fluorescent Protein (EGFP) has been used for assaying a wide range of biological activities ranging from gene expression, or localization of target proteins through to intermolecular interactions. However, over-production of this protein in Escherichia coli has resulted in the presence of inclusion bodies, which complicates recovery of the protein in significant quantities. In this paper, we describe a single-step method for isolating the protein from a Glutathione-S-Transferase (GST) fusion protein, release of the EGFP protein from the fusion was demonstrated using a biotinylated variant of Human Rhinovirus 14 3C protease that we have also constructed. We also suggest the potential uses of the biotinylated protease for bionanotechnology and synthetic biology.

journal_name

Protein Expr Purif

journal_title

Protein expression and purification

authors

Youell J,Fordham D,Firman K

doi

10.1016/j.pep.2011.05.003

subject

Has Abstract

pub_date

2011-10-01 00:00:00

pages

258-62

issue

2

eissn

1046-5928

issn

1096-0279

pii

S1046-5928(11)00117-3

journal_volume

79

pub_type

杂志文章

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