Abstract：
:Streptococcal protein G has been used extensively for the purification of antibodies using the interaction of the Fc region with protein G. Many antibodies also interact with protein G through a low-affinity binding site for the Fab region. The exploitation of this low-affinity interaction for the purification of Fab' fragments is described here. Chimeric mouse-human B72.3 Fab' and F(ab')2 fragments were expressed by CHO cells and purified from CHO cell supernatant using protein G-Sepharose. Since chimeric B72.3 Fab' bound weakly to the protein G-Sepharose it could be separated from F(ab')2 and eluted with a pH 7 wash whereas B72.3 F(ab')2 required elution at pH 2. Both Fab' and F(ab')2 were recovered with full immunoreactivity and could be further purified using gel-filtration chromatography to greater than 99% purity. This method allows the simple purification of directly expressed Fab' or F(ab')2 fragments from CHO cell supernatant.
journal_name
Protein Expr Purifjournal_title
Protein expression and purificationauthors
Proudfoot KA,Torrance C,Lawson AD,King DJdoi
10.1016/s1046-5928(05)80037-3keywords:
subject
Has Abstractpub_date
1992-10-01 00:00:00pages
368-73issue
5eissn
1046-5928issn
1096-0279pii
S1046-5928(05)80037-3journal_volume
3pub_type
杂志文章abstract：:The basidiomycete Phanerochaete chrysosporium produces several isoforms of lignin peroxidase, which catalyzes the oxidative depolymerization of lignin To date, ion-exchange chromatography and preparative isoelectric focusing (IEF) have been commonly used for isolation of lignin peroxidase isoenzymes. In this work we h...
journal_title：Protein expression and purification
pub_type： 杂志文章
doi：10.1006/prep.1995.1044
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abstract：:An alpha-amylase has been purified from the thermophilic fungus Scytalidium thermophilum. A ninefold purification was achieved in a single step using fluidized bed chromatography wherein alginate was used as the affinity matrix. There are at least two isoenzymes as shown by concanavalin A (Con A)-agarose column chroma...
journal_title：Protein expression and purification
pub_type： 杂志文章
doi：10.1006/prep.2000.1308
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journal_title：Protein expression and purification
pub_type： 杂志文章
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journal_title：Protein expression and purification
pub_type： 杂志文章
doi：10.1006/prep.1997.0840
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journal_title：Protein expression and purification
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doi：10.1016/j.pep.2004.07.019
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abstract：:Cyclophilins are highly conserved proteins associated with peptidyl-prolyl cis-trans isomerase activity (PPIase). The present study was designed to analyze the biological activity of recombinant cyclophilin from the marine red algae Pyropia yezoensis (PyCyp). The cyclophilin gene from P. yezoensis was cloned into the ...
journal_title：Protein expression and purification
pub_type： 杂志文章
doi：10.1016/j.pep.2020.105636
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journal_title：Protein expression and purification
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journal_title：Protein expression and purification
pub_type： 杂志文章
doi：10.1016/j.pep.2010.11.019
更新日期：2011-05-01 00:00:00
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journal_title：Protein expression and purification
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doi：10.1016/j.pep.2014.12.016
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journal_title：Protein expression and purification
pub_type： 杂志文章
doi：10.1016/s1046-5928(02)00035-9
更新日期：2002-08-01 00:00:00
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journal_title：Protein expression and purification
pub_type： 杂志文章
doi：10.1016/j.pep.2005.04.011
更新日期：2005-08-01 00:00:00
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journal_title：Protein expression and purification
pub_type： 杂志文章
doi：10.1016/j.pep.2008.12.012
更新日期：2009-05-01 00:00:00
abstract：:PLD's (Phospholipases D) are ubiquitously expressed proteins involved in many transphosphatidylation reactions. They have a bi-lobed structure composed by two similar domains which at their interface reconstitute the catalytic site through the association of the two conserved HxKx(4)Dx(6)GSxN motifs. PLD1 interacts wi...
journal_title：Protein expression and purification
pub_type： 杂志文章
doi：10.1016/j.pep.2008.02.012
更新日期：2008-06-01 00:00:00
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journal_title：Protein expression and purification
pub_type： 杂志文章
doi：10.1006/prep.2001.1582
更新日期：2002-04-01 00:00:00
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journal_title：Protein expression and purification
pub_type： 杂志文章
doi：10.1006/prep.1996.0087
更新日期：1996-09-01 00:00:00
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journal_title：Protein expression and purification
pub_type： 杂志文章
doi：10.1016/j.pep.2014.03.011
更新日期：2014-07-01 00:00:00
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journal_title：Protein expression and purification
pub_type： 杂志文章
doi：10.1006/prep.1993.1070
更新日期：1993-12-01 00:00:00
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journal_title：Protein expression and purification
pub_type： 杂志文章
doi：10.1016/j.pep.2006.06.011
更新日期：2006-11-01 00:00:00
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journal_title：Protein expression and purification
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更新日期：2014-10-01 00:00:00
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journal_title：Protein expression and purification
pub_type： 杂志文章
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journal_title：Protein expression and purification
pub_type： 杂志文章
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journal_title：Protein expression and purification
pub_type： 杂志文章
doi：10.1016/j.pep.2013.07.016
更新日期：2013-10-01 00:00:00
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journal_title：Protein expression and purification
pub_type： 杂志文章
doi：10.1016/j.pep.2017.04.011
更新日期：2017-07-01 00:00:00
abstract：:The present report documents an improved enzyme assay method for the mammalian L-alanine:4,5-dioxovalerate transaminase which is of significant utility in work with crude tissue homogenates, cell cultures, or purified enzyme preparations. We also describe a new and rapid purification procedure for this enzyme from rat...
journal_title：Protein expression and purification
pub_type： 杂志文章
doi：10.1006/prep.1994.1072
更新日期：1994-12-01 00:00:00
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journal_title：Protein expression and purification
pub_type： 杂志文章
doi：10.1016/j.pep.2015.12.010
更新日期：2016-04-01 00:00:00
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journal_title：Protein expression and purification
pub_type： 杂志文章
doi：10.1006/prep.1996.0079
更新日期：1996-08-01 00:00:00
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journal_title：Protein expression and purification
pub_type： 杂志文章
doi：10.1006/prep.2001.1490
更新日期：2001-10-01 00:00:00
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journal_title：Protein expression and purification
pub_type： 杂志文章
doi：10.1016/j.pep.2014.02.015
更新日期：2014-06-01 00:00:00
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journal_title：Protein expression and purification
pub_type： 杂志文章
doi：10.1006/prep.1996.0103
更新日期：1996-11-01 00:00:00
abstract：:Sarcotoxin IA is an antibacterial peptide that is secreted by a meat-fly Sarcophaga peregrina larva in response to a hypodermic injury or bacterial infection. This peptide is highly toxic against a broad spectrum of both Gram-positive and Gram-negative bacteria and lethal to microbes even at nanomolar concentrations. ...
journal_title：Protein expression and purification
pub_type： 杂志文章
doi：10.1016/s1046-5928(02)00697-6
更新日期：2003-04-01 00:00:00
