High-level expression of human lactoferrin in the milk of goats by using replication-defective adenoviral vectors.

abstract：:The immunosuppressive drug FK506 binds its targets FK506-binding protein (FKBP) family and modulates cellular processes. Recent studies demonstrated that FK506 shows anti-malaria effects. Newly identified FK506-binding protein 35 from Plasmodium falciparum (PfFKBP35) is assumed to be the molecular target of FK506 in t...

journal_title：Protein expression and purification

authors： Yoon HR,Kang CB,Chia J,Tang K,Yoon HS

更新日期：2007-05-01 00:00:00

abstract：:Ketol-acid reductoisomerase (EC 1.1.1.86) catalyzes the conversion of 2-aceto-2-hydroxyacids to 2-keto-3-hydroxyacids and their subsequent reduction by NADPH to 2,3-dihydroxyacids. The gene encoding the Escherichia coli enzyme was cloned and expressed as a hexahistidine-tagged fusion protein and the recombinant enzyme...

journal_title：Protein expression and purification

authors： Hill CM,Duggleby RG

更新日期：1999-02-01 00:00:00

abstract：:Streptomyces coelicolor is a soil-dwelling bacterium that undergoes an intricate, saprophytic lifecycle. The bacterium takes up exogenous nucleosides for nucleic acid synthesis or use as carbon and energy sources. However, nucleosides must pass through the membrane with the help of transporters. In the present work, t...

journal_title：Protein expression and purification

authors： Li F,Liang J,Wang W,Zhou X,Deng Z,Wang Z

更新日期：2015-05-01 00:00:00

abstract：:A purification scheme based on hydrophobic interaction chromatography was developed to separate inactivated foot-and-mouth disease virus (FMDV) from crude supernatant. About 92% recovery and 8.8-fold purification were achieved on Butyl Sepharose 4 FF. Further purification on Superdex 200 resulted in another 29-fold pu...

journal_title：Protein expression and purification

authors： Li H,Yang Y,Zhang Y,Zhang S,Zhao Q,Zhu Y,Zou X,Yu M,Ma G,Su Z

更新日期：2015-09-01 00:00:00

abstract：:Infections caused by Acinetobacter baumannii have emerged as a significant clinical problem due to the increase in infections caused by antibiotic resistant strains. A. baumannii OmpA is a highly conserved membrane protein that has multiple roles in interacting with the host during infection, and thus represents an at...

journal_title：Protein expression and purification

authors： McConnell MJ,Pachón J

更新日期：2011-05-01 00:00:00

abstract：:The gene that was inferred to encode the TyrR protein of Haemophilus influenzae Rd was synthesized by polymerase chain reaction and inserted into a T7-based expression vector. Methods were developed to overexpress the TyrR protein of H. influenzae in Escherichia coli and to purify the protein on a large scale. Both in...

journal_title：Protein expression and purification

authors： Zhu Q,Zhao S,Somerville RL

更新日期：1997-07-01 00:00:00

abstract：:Splicing factor proline- and glutamine-rich (SFPQ) is an RNA-binding protein, playing significant roles in gene regulation and subnuclear body formation. Our recent serendipitous discovery showed that SFPQ binds zinc directly and forms an infinite polymer that is induced by zinc binding to the protein. The zinc-induce...

journal_title：Protein expression and purification

authors： Lim YW,Lee M

更新日期：2020-07-01 00:00:00

abstract：:Listeriolysin O (LLO) is a cholesterol-binding sulfhydryl-activated hemolysin encoded by Listeria monocytogenes hlyA gene. After analyzing the nucleotide coding sequence of this gene from the ATCC 9525 L. monocytogenes strain, we cloned it in a pET vector for expression in Escherichia coli. Thanks to the optimization ...

journal_title：Protein expression and purification

authors： Giammarini C,Andreoni F,Amagliani G,Casiere A,Barocci S,Magnani M

更新日期：2003-03-01 00:00:00

abstract：:A full-length hexokinase cDNA was cloned from Solanum chacoense, a wild relative of the cultivated potato. Analysis of the predicted primary sequence suggested that the protein product, ScHK2, may be targeted to the secretory pathway and inserted in the plant plasma membrane, facing the cytosol. ScHK2 was expressed as...

journal_title：Protein expression and purification

authors： Claeyssen E,Wally O,Matton DP,Morse D,Rivoal J

更新日期：2006-05-01 00:00:00

abstract：:Human granulocyte-macrophage colony-stimulating factor (GM-CSF), a hemopoietic growth factor, was produced and secreted from tobacco cell suspensions. The GM-CSF cDNA was carried by a binary vector under the control of the CaMV 35S promoter and the T7 terminator. In addition, a 5'-nontranslated region from the tobacco...

journal_title：Protein expression and purification

authors： James EA,Wang C,Wang Z,Reeves R,Shin JH,Magnuson NS,Lee JM

更新日期：2000-06-01 00:00:00

abstract：:Dehydrodolichyl diphosphate synthase (DDPPs) catalyzes the sequential condensation of isopentenyl diphosphate with farnesyl diphosphate to synthesize long-chain dehydrodolichyl diphosphate, which serves as a precursor of glycosyl carrier in glycoprotein biosynthesis in eukaryotes. To perform kinetic and structural stu...

journal_title：Protein expression and purification

authors： Chang SY,Tsai PC,Tseng CS,Liang PH

更新日期：2001-12-01 00:00:00

abstract：:The catalase gene of Psychrobacter sp. T-3 was cloned, and the gene product (PktA) was overexpressed in Escherichia coli. The specific activity of the purified PktA was slightly lower than that of the native purified enzyme obtained from Psychrobacter sp. T-3. Spectrophotometric measurements of the purified enzymes su...

journal_title：Protein expression and purification

authors： Kimoto H,Matsuyama H,Yumoto I,Yoshimune K

更新日期：2008-06-01 00:00:00

abstract：:The basidiomycete Phanerochaete chrysosporium produces several isoforms of lignin peroxidase, which catalyzes the oxidative depolymerization of lignin To date, ion-exchange chromatography and preparative isoelectric focusing (IEF) have been commonly used for isolation of lignin peroxidase isoenzymes. In this work we h...

journal_title：Protein expression and purification

authors： Ollikka P,Leppänen VM,Anttila T,Suominen I

更新日期：1995-06-01 00:00:00

abstract：:An active form of a single-chain antibody fragment (scFv) from the murine monoclonal antibody ABL-1, which is specific for B-cell-activating factor of the TNF family, was produced in Escherichia coli. The complementary DNAs encoding the variable regions of the heavy chain (VH) and light chain (VL) were connected by a ...

journal_title：Protein expression and purification

authors： Cao P,Tang XM,Guan ZB,Diao ZY,Zhang SQ

更新日期：2005-10-01 00:00:00

abstract：:General procedures for the rapid, large-scale purification of recombinant Lactobacillus casei thymidylate synthase and its mutants have been established. An effective method employs sequential phosphocellulose and hydroxylapatite chromatography. Crude cell extracts are directly applied to phosphocellulose, and the enz...

journal_title：Protein expression and purification

authors： Kealey JT,Santi DV

更新日期：1992-10-01 00:00:00

abstract：:The complement regulatory protein (CRP) of Trypanosoma cruzi is a developmentally regulated glycosylphosphatidylinositol (GPI)-anchored membrane protein that protects the parasite from complement-mediated killing, and is an important virulence determinant of the microorganism. CRP binds human complement components C3b...

journal_title：Protein expression and purification

authors： Beucher M,Meira WS,Zegarra V,Galvão LM,Chiari E,Norris KA

更新日期：2003-01-01 00:00:00

abstract：:The role of the termini of protein sequences is often perturbed by remnant amino acids after the specific protease cleavage of the affinity tags and/or by the amino acids encoded by the plasmid at/around the restriction enzyme sites used to insert the genes. Here we describe a method for affinity purification of a met...

journal_title：Protein expression and purification

authors： Abd Elhameed HAH,Hajdu B,Balogh RK,Hermann E,Hunyadi-Gulyás É,Gyurcsik B

更新日期：2019-07-01 00:00:00

abstract：:Pseudolysin is the extracellular elastase of Pseudomonas aeruginosa and belongs to the thermolysin-like family of metallopeptidases. Pseudolysin has been identified as a robust drug target and a biotechnologically important enzyme in the tanning industry. Previous attempts to purify active pseudolysin from P. aerugino...

journal_title：Protein expression and purification

authors： Odunuga OO,Adekoya OA,Sylte I

更新日期：2015-09-01 00:00:00

abstract：:The transcription factor Sp1 is a regulator of TATA-less genes. It belongs to the Cys₂-His₂ zinc finger domain-containing family. A zebrafish cDNA encoding a peptide homologous to mammalian Sp1 was cloned and inserted into a pET43.1a vector and expressed in Escherichia coli Rosetta (DE3) cells as a Nus-His-tag fusion ...

journal_title：Protein expression and purification

authors： Lin CJ,Hsiao TH,Chung YS,Chang WN,Yeh TM,Chen BH,Fu TF

更新日期：2011-03-01 00:00:00

abstract：:A maize dehydrin with an apparent molecular weight of 20 kDa was purified from whole kernels of maize inbred line G50. Kernels were ground in a seed mill, stirred overnight in extraction buffer, and centrifuged to extract soluble proteins. The sample was heated to 89 degrees C and centrifuged to remove heat-insoluble ...

journal_title：Protein expression and purification

authors： Ceccardi TL,Meyer NC,Close TJ

更新日期：1994-06-01 00:00:00

abstract：:Human small nuclear (sn) RNA genes are transcribed by either RNA polymerase II or III depending upon the arrangement of their core promoter elements. Regardless of polymerase specificity, these genes share a requirement for a general transcription factor called the snRNA activating protein complex or SNAP(C). This mul...

journal_title：Protein expression and purification

authors： Hanzlowsky A,Jelencic B,Jawdekar G,Hinkley CS,Geiger JH,Henry RW

更新日期：2006-08-01 00:00:00

abstract：:The recently classified group III trypsins include members like Atlantic cod (Gadus morhua) trypsin Y as well as seven analogues from other cold-adapted fish species. The eight group III trypsins have been characterized from their cDNAs and deduced amino acid sequences but none of the enzymes have been isolated from t...

journal_title：Protein expression and purification

authors： Pálsdóttir HM,Gudmundsdóttir A

更新日期：2007-02-01 00:00:00

abstract：:Proteomics has become a major focus as researchers attempt to understand the vast amount of genomic information. Protein complexity makes identifying and understanding gene function inherently difficult. The challenge of studying proteins in a global way is driving the development of new technologies for systematic an...

journal_title：Protein expression and purification

authors： Lesley SA

更新日期：2001-07-01 00:00:00

abstract：:The A(280)/A(260) ratio of a purified protein is frequently used as an indication of the purity of the preparation with respect to nucleic acids. We show here that for low-molecular-weight recombinant proteins purified from Escherichia coli, a low A(280)/A(260) ratio can also result from contamination with UDP-linked ...

journal_title：Protein expression and purification

authors： Ram MK,Andrade LJ,Phillips TB,van Schravendijk MR

更新日期：1999-11-01 00:00:00

abstract：:Alphavirus nonstructural protein nsP1 possesses distinct methyltransferase (MTase) and guanylyltransferase (GTase) activities involved in the capping of viral RNAs. In alphaviruses, the methylation of GTP occurs before RNA transguanylation and nsP1 forms a covalent complex with m(7)GMP unlike the host mRNA guanylyltra...

journal_title：Protein expression and purification

authors： Tomar S,Narwal M,Harms E,Smith JL,Kuhn RJ

更新日期：2011-10-01 00:00:00

abstract：:p53 protein is an important regulation factor that can bind to p53 mRNA to regulate its translation in human and murine. To determine if a similar interaction exists in zebrafish and if the interaction affects zebrafish development, we cloned and expressed p53 protein from zebrafish in Escherichia coli. Soluble p53 pr...

journal_title：Protein expression and purification

authors： Zhao X,Liu M,Wu N,Ding L,Liu H,Lin X

更新日期：2010-08-01 00:00:00

abstract：:The production of a single-chain variable fragment (scFv) antibody against bovine ribonuclease A in the cytoplasm of Escherichia coli trxB/gor double mutant was investigated. Previous reports have shown that the thioredoxin (Trx) protein fusion strategy is useful for the correct folding of scFvs and that the expressio...

journal_title：Protein expression and purification

authors： Sonoda H,Kumada Y,Katsuda T,Yamaji H

更新日期：2010-04-01 00:00:00

abstract：:The alpha-subunit of eukaryotic initiation factor eIF2 (eIF2alpha) plays an important role in the regulation of mRNA translation through modulation of the interaction of eIF2 and a second initiation factor, eIF2B. The interaction of the two proteins is regulated in vivo by phosphorylation of eIF2alpha at Ser51. In the...

journal_title：Protein expression and purification

authors： Kimball SR,Horetsky RL,Jagus R,Jefferson LS

更新日期：1998-04-01 00:00:00

abstract：:Na+, K+-ATPase beta2 subunit (NKA1b2) is not only a regulator of Na+, K+-ATPase, but also functions in the interaction between neuron and glia cells as a Ca2+-dependent adhesion molecule. To further study the function of NKA1b2, the anti-NKA1b2 polyclonal antibody was prepared to recognize the outer-membrane carboxyl ...

journal_title：Protein expression and purification

authors： Chen TF,Zhang YL,Xu WL,Li ZQ,Hou B,Wang CL,Fan M,Qian LJ,Zhou RP,Zhang CG

更新日期：2004-09-01 00:00:00

abstract：:The cyclin-dependent kinase-activating kinase (CAK) catalyzes the phosphorylation of the cyclin-dependent protein kinases (CDKs) on a threonine residue (Thr160 in human CDK2). The reaction is an obligatory step in the activation of the CDKs. In higher eukaryotes, the CAK complex has been characterized in two forms. Th...

journal_title：Protein expression and purification

authors： Lawrie AM,Tito P,Hernandez H,Brown NR,Robinson CV,Endicott JA,Noble ME,Johnson LN

更新日期：2001-11-01 00:00:00