Rapid purification method for human SFPQ by implementing zinc-induced polymerization.

abstract：:The murine monoclonal antibody 125E11 is an IgG which recognizes PreS1(21-47) fragment of large hepatitis B surface antigen. It has been successfully used for clinical detection of HBV virion in serum of hepatitis B patients. In present study, the genes of variable region in heavy chain (VH) and light chain (VL) of 12...

journal_title：Protein expression and purification

authors： Yang X,Hu W,Li F,Xia H,Zhang Z

更新日期：2005-06-01 00:00:00

abstract：:Influenza A virus displays one of the highest infection rates of all human viruses and therefore represents a severe human health threat associated with an important economical challenge. Influenza matrix protein 2 (M2) is a membrane protein of the viral envelope that forms a proton selective ion channel. Here we repo...

journal_title：Protein expression and purification

authors： Desuzinges Mandon E,Traversier A,Champagne A,Benier L,Audebert S,Balme S,Dejean E,Rosa Calatrava M,Jawhari A

更新日期：2017-03-01 00:00:00

abstract：:Serine hydroxymethyltransferase (SHMT) plays a key role in cell physiology as it participates in the different interconversion pathway of folate coenzymes, provides almost exclusively folate one carbon fragments for the biosynthesis of a variety of end products. For the first time, Mycobacterium leprae glyA gene, enco...

journal_title：Protein expression and purification

authors： Sharma S,Bhakuni V

更新日期：2007-09-01 00:00:00

abstract：:A recombinant H(C) fragment of botulinum neurotoxin, serotype A (rBoNTA(H(C))), has been successfully expressed in a Mut(+) strain of the methylotrophic yeast Pichia pastoris for use as an antigen in a proposed human vaccine. Fermentation employed glycerol batch, glycerol-fed batch, and methanol-fed batch phases to ac...

journal_title：Protein expression and purification

authors： Potter KJ,Zhang W,Smith LA,Meagher MM

更新日期：2000-08-01 00:00:00

abstract：:We present a novel efficient procedure for high level purification of human IGFBP-3. Insulin-like growth factor-binding proteins (IGFBPs) are key regulators of insulin-like growth factor mediated signal transduction and thereby can profoundly influence cellular phenotypes. Certain IGFBPs, including IGFBP-3, have also ...

journal_title：Protein expression and purification

authors： Pircher H,Matscheski A,Laich A,Hermann M,Moser B,Viertler HP,Micutkova L,Lindner H,Sarg B,Zwerschke W,Jansen-Dürr P

更新日期：2010-06-01 00:00:00

abstract：:We have developed a family of cloning vectors that direct expression of fusion proteins that mimic aggregated immunoglobulin (IgG) (AIG) and immune complex function with respect to their interactions with FcgammaR and that allow for the inclusion and targeting of a second protein domain to cells expressing FcgammaR. T...

journal_title：Protein expression and purification

authors： White DM,Jensen MA,Shi X,Qu Zx,Arnason BG

更新日期：2001-04-01 00:00:00

abstract：:α-N-Acetylgalactosaminidase (α-GalNAc-ase; EC.3.2.1.49) is an exoglycosidase specific for the hydrolysis of terminal α-linked N-acetylgalactosamine in various sugar chains. The cDNA corresponding to the α-GalNAc-ase gene was cloned from Aspergillus niger, sequenced, and expressed in the yeast Saccharomyces cerevisiae....

journal_title：Protein expression and purification

authors： Mrázek H,Benada O,Man P,Vaněk O,Křen V,Bezouška K,Weignerová L

更新日期：2012-01-01 00:00:00

abstract：:The full-length cDNA that encodes the hemolytic toxin Avt-I, with 226 amino acids, from the venomous sea anemone Actineria villosa has been cloned using the oligo-capping method. The cDNA contains 681bp open reading frame and its predicted amino acid sequences revealed that Avt-I was basic polypeptides without cystein...

journal_title：Protein expression and purification

authors： Uechi G,Toma H,Arakawa T,Sato Y

更新日期：2005-04-01 00:00:00

abstract：:The metalloprotease PrtV from Vibrio cholerae serves an important function for the bacteria's ability to invade the mammalian host cell. The protein belongs to the family of M6 proteases, with a characteristic zinc ion in the catalytic active site. PrtV constitutes a 918 amino acids (102 kDa) multidomain pre-pro-prote...

journal_title：Protein expression and purification

authors： Edwin A,Grundström C,Wai SN,Ohman A,Stier G,Sauer-Eriksson AE

更新日期：2014-04-01 00:00:00

abstract：:L-Threonine dehydrogenase was purified 10,000-fold to a specific activity approximately 300 mumol.min-1.mg-1 protein from porcine liver mitochondria. Purification to apparent homogeneity was achieved by sequential chromatography on DEAE Sepharose FF, Affi-Gel Blue, Sephacryl S-200, Matrex Gel Red A, and Matrex Gel Gre...

journal_title：Protein expression and purification

authors： Kao YC,Davis L

更新日期：1994-10-01 00:00:00

abstract：:Molecular-level investigation of proteins is increasingly important to researchers trying to understand the mechanisms of signal transmission. Heterotrimeric G proteins control the activation of many critical signal transmission cascades and are also implicated in numerous diseases. As part of a longer-term investigat...

journal_title：Protein expression and purification

authors： Maly J,Crowhurst KA

更新日期：2012-08-01 00:00:00

abstract：:We have investigated the suitability of Pichia pastoris as an expression system for the candidate therapeutic protein, Sonic hedgehog fused to an immunoglobulin Fc domain (Shh-Fc). Sonic hedgehog is a morphogen protein involved in the patterning of a wide range of tissues during animal embryogenesis. The presence of S...

journal_title：Protein expression and purification

authors： Shapiro RI,Wen D,Levesque M,Hronowski X,Gill A,Garber EA,Galdes A,Strauch KL,Taylor FR

更新日期：2003-06-01 00:00:00

abstract：:Three human cytochrome P450 1A1 (CYP1A1) allelic variants, namely wild-type (CYP1A1.1), CYP1A1.2 (I462V), and CYP1A1.4 (T461N), were expressed as C-terminal His-tagged fusions including a thrombin cleavage site in Spodoptera frugiperda insect cells by baculovirus infection. The variants were expressed with 30-90 nmol ...

journal_title：Protein expression and purification

authors： Chernogolov A,Behlke J,Schunck WH,Roots I,Schwarz D

更新日期：2003-04-01 00:00:00

abstract：:A method is presented to produce large amounts of Bcl-2 and Bcl-x(L), two anti-apoptotic proteins of considerable biomedical interest. Expression constructs were prepared in which the Escherichia coli protein TolAIII, known to promote over expression of soluble product, was added to the N-terminus of Bcl-2 or Bcl-x(L)...

journal_title：Protein expression and purification

authors： Nedelkina S,Gokce I,Ridley H,Weckerle C,Magnin T,Vallette F,Pattus F,Lakey JH,Bechinger B

更新日期：2008-08-01 00:00:00

abstract：:Pokeweed antiviral protein (PAP)-I from the spring leaves of Phytolacca americana is a naturally occurring RNA-depurinating enzyme with broad-spectrum antiviral activity. Interest in PAP is growing due to its use as a potential anti-HIV agent. However, the clinical use of native PAP is limited due to inherent difficul...

journal_title：Protein expression and purification

authors： Rajamohan F,Doumbia SO,Engstrom CR,Pendergras SL,Maher DL,Uckun FM

更新日期：2000-03-01 00:00:00

abstract：:The recombinant human malonyl-CoA decarboxylase (hMCD) was overexpressed in Escherichia coli with and without the first 39 N-terminal amino acids via a cleavable MBP-fusion construct. Proteolytic digestion using genenase I to remove the MBP-fusion tag was optimized for both the full length and truncated hMCD. The apo-...

journal_title：Protein expression and purification

authors： Zhou D,Yuen P,Chu D,Thon V,McConnell S,Brown S,Tsang A,Pena M,Russell A,Cheng JF,Nadzan AM,Barbosa MS,Dyck JR,Lopaschuk GD,Yang G

更新日期：2004-04-01 00:00:00

abstract：:Botulinum neurotoxin light chain (BoNT LC, 50 kDa) is responsible for the zinc endopeptidase activity specific for proteins of neuroexocytosis apparatus. We describe the expression of recombinant type A BoNT LC in Escherichia coli as well as the purification and characterization of the recombinant protein. A high leve...

journal_title：Protein expression and purification

authors： Li L,Singh BR

更新日期：1999-12-01 00:00:00

abstract：:Conkunitzin-S1 from the cone snail Conus striatus is the first member of a new neurotoxin family with a canonical Kunitz domain fold. Conk-S1 is 60 amino acids long and lacks one of the three conserved disulfide bonds typically found in Kunitz domain modules. It binds specifically to voltage activated potassium channe...

journal_title：Protein expression and purification

authors： Bayrhuber M,Graf R,Ferber M,Zweckstetter M,Imperial J,Garrett JE,Olivera BM,Terlau H,Becker S

更新日期：2006-06-01 00:00:00

abstract：:A fairly rapid and improved method for producing large amounts of highly pure apoaequorin, the apoprotein of aequorin which emits light on binding Ca2+, is described. The method consists of fusing the gene of the outer membrane protein A (ompA) secretion signal peptide of Escherichia coli to the apoaequorin gene and e...

journal_title：Protein expression and purification

authors： Inouye S,Zenno S,Sakaki Y,Tsuji FI

更新日期：1991-04-01 00:00:00

abstract：:The covalent addition of 4-amino-4-deoxy-L-arabinose (L-Ara4N) groups to lipid A, which resides in the outer membranes of bacteria such as Salmonella typhimurium and Escherichia coli, is the final step in the polymyxin-resistance pathway in these organisms. This modification is catalyzed by the inner membrane protein ...

journal_title：Protein expression and purification

authors： Bretscher LE,Morrell MT,Funk AL,Klug CS

更新日期：2006-03-01 00:00:00

abstract：:The superoxide dismutases (EC 1.15.1.1) are a family of enzymes that catalyze the dismutation of superoxide radical anion to dioxygen and hydrogen peroxide. The active site contains a critical metal ion such as manganese, iron, or copper. The copper-containing protein also has one zinc ion bound per subunit. The stand...

journal_title：Protein expression and purification

authors： Sutter B,Bounds PL,Koppenol WH

更新日期：2000-06-01 00:00:00

abstract：:Transcription regulation in the cell occurs in the context of chromatin. It follows that a thorough investigation of the mechanism of transcription regulation must take into account the role of chromatin structure. Through classical and molecular genetic experiments in yeast, great strides have been made in understand...

journal_title：Protein expression and purification

authors： Pilon J,Terrell A,Laybourn PJ

更新日期：1997-06-01 00:00:00

abstract：:The fusion protein consisting of human Fas receptor extracellular domain and human IgG1 heavy chain Fc domain (hFasRECD-Fc) is a medically important protein that potentially has therapeutic uses. The fusion gene composed of a synthetic human Fas receptor extracellular domain gene and the cDNA encoding human IgG1 heavy...

journal_title：Protein expression and purification

authors： Muraki M,Honda S

更新日期：2010-10-01 00:00:00

abstract：:The B-subunits of replicative DNA polymerases belong to the superfamily of calcineurin-like phosphoesterases and are conserved from Archaea to humans. Recently we and others have shown that the B-subunit (DP1) of the archaeal family D DNA polymerase is responsible for proofreading 3'-5' exonuclease activity. The simil...

journal_title：Protein expression and purification

authors： Jokela M,Raki M,Heikkinen K,Sepponen K,Eskelinen A,Syväoja JE

更新日期：2005-09-01 00:00:00

abstract：:Hepatitis C virus encodes two enveloped glycoproteins, E1 and E2, which are involved in viral attachment and entry into target cells. We have obtained in insect cells infected by recombinant baculovirus a chimeric secreted recombinant protein, E1(341)E2(661,) containing the ectodomains of E1 and E2. The described proc...

journal_title：Protein expression and purification

authors： Tello D,Rodríguez-Rodríguez M,Yélamos B,Gómez-Gutiérrez J,Ortega S,Pacheco B,Peterson DL,Gavilanes F

更新日期：2010-06-01 00:00:00

abstract：:Lactase deficiency problem discourages many adults from consuming milk as a major source of micro- and macronutrients. Enzymatic hydrolysis of lactose is an ideal solution for this problem but such processing adds significant costs. In this study, a cold active β-galactosidase from Planococcus sp-L4 (bgal) was optimiz...

journal_title：Protein expression and purification

authors： Mahdian SM,Karimi E,Tanipour MH,Parizadeh SM,Ghayour-Mobarhan M,Bazaz MM,Mashkani B

更新日期：2016-09-01 00:00:00

abstract：:Recombinant human interleukin-5 (rhIL-5) was expressed in baculovirus-infected insect cells and purified to homogeneity from the culture medium in a single chromatographic step. Beginning with a cDNA encoding the full-length precursor form of human IL-5, including the authentic secretory leader sequence, recombinant b...

journal_title：Protein expression and purification

authors： Brown PM,Scheid MP,O'Neill GP,Tagari PC,Nicholson DW

更新日期：1995-02-01 00:00:00

abstract：:The erm proteins confer resistance to the MLS (macrolide-lincosamide-streptogramin B) antibiotics in various microorganisms, including pathogens, through dimethylation of a single adenine residue (A2085: Bacillus subtilis coordinate) of the 23S rRNA to reduce the affinity of antibiotics, thereby enabling the cells to ...

journal_title：Protein expression and purification

authors： Jin HJ,Yang YD

更新日期：2002-06-01 00:00:00

abstract：:Cysteine-rich intestinal protein (CRIP) is a small, 8.5-kDa protein with one double zinc-finger motif called a LIM domain. It is very abundant in intestine and some immune cells in rodents, and expression is influenced by development and the immune response. We have cloned a human CRIP cDNA from human small intestine ...

journal_title：Protein expression and purification

authors： Khoo C,Blanchard RK,Sullivan VK,Cousins RJ

更新日期：1997-04-01 00:00:00

abstract：:Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), which is capable of selectively inducing apoptosis of cancer cells, is a potential targeted drug for cancer therapy. The TRAIL protein induces apoptosis only in trimeric form. However, the recombinant soluble TRAIL (sTRAIL) trimer has low stability and a...

journal_title：Protein expression and purification

authors： Wang B,Wang Z,Yan J,Wang L,Wang Z,Wu J,Zhang H,Wu H,Kong W,Yu B,Yu X

更新日期：2015-11-01 00:00:00