Expression of biologically active recombinant pokeweed antiviral protein in methylotrophic yeast Pichia pastoris.

abstract：:A method is presented to produce large amounts of Bcl-2 and Bcl-x(L), two anti-apoptotic proteins of considerable biomedical interest. Expression constructs were prepared in which the Escherichia coli protein TolAIII, known to promote over expression of soluble product, was added to the N-terminus of Bcl-2 or Bcl-x(L)...

journal_title：Protein expression and purification

authors： Nedelkina S,Gokce I,Ridley H,Weckerle C,Magnin T,Vallette F,Pattus F,Lakey JH,Bechinger B

更新日期：2008-08-01 00:00:00

abstract：:NIP1, the product of the avirulence gene AvrRrs1 from Rhynchosporium secalis, a fungal pathogen of barley, is a small secreted cysteine-rich protein. This protein is essential for the specific recognition of the fungus by host plants carrying the complementary resistance gene Rrs1. Different heterologous expression sy...

journal_title：Protein expression and purification

authors： Gierlich A,van 't Slot KA,Li VM,Marie C,Hermann H,Knogge W

更新日期：1999-10-01 00:00:00

abstract：:Infections caused by Acinetobacter baumannii have emerged as a significant clinical problem due to the increase in infections caused by antibiotic resistant strains. A. baumannii OmpA is a highly conserved membrane protein that has multiple roles in interacting with the host during infection, and thus represents an at...

journal_title：Protein expression and purification

authors： McConnell MJ,Pachón J

更新日期：2011-05-01 00:00:00

abstract：:The major capsid protein L1 of human papillomavirus (HPV) contains the immunodominant neutralization epitopes of the virus and can auto-assembles to form virus-like particles (VLPs). Therefore, HPV L1 capsid proteins have been well investigated as potential vaccine candidates. To express large quantities of human papi...

journal_title：Protein expression and purification

authors： Ma Z,Chen B,Zhang F,Yu M,Liu T,Liu L

更新日期：2007-11-01 00:00:00

abstract：:Human β-glucuronidase (GUS; EC 3.2.1.31) is a lysosomal enzyme that catalyzes the hydrolysis of β-d-glucuronic acid residues from the non-reducing termini of glycosaminoglycans. Impairment in GUS function leads to the metabolic disorder mucopolysaccharidosis type VII, also known as Sly syndrome. We produced GUS from a...

journal_title：Protein expression and purification

authors： Fratz-Berilla EJ,Ketcham SA,Parhiz H,Ashraf M,Madhavarao CN

更新日期：2017-12-01 00:00:00

abstract：:BTK-2, a 32 residue scorpion toxin initially identified in the venom of red Indian scorpion Mesobuthus tamulus was cloned, overexpressed and purified using Cytochrome b(5) fusion protein system developed in our laboratory. The synthetic gene coding for the peptide was designed taking into account optimal codon usage b...

journal_title：Protein expression and purification

authors： Kumar GS,Sarma SP

更新日期：2010-04-01 00:00:00

abstract：:The ELR-CXC chemokines are important to neutrophil inflammation in many acute and chronic diseases. Among them, CXCL8 (interleukin-8, IL-8), binds to both the CXCR1 and CXCR2 receptors with high affinity and the expression levels of CXCL8 are elevated in many inflammatory diseases. Recently, an analogue of human CXCL8...

journal_title：Protein expression and purification

authors： Cheng HT,Huang KC,Yu HY,Gao KJ,Zhao X,Li F,Town J,Gordon JR,Cheng JW

更新日期：2008-09-01 00:00:00

abstract：:Keratinocyte growth factor (KGF) is a member of the fibroblast growth factor family that acts specifically on epithelial cells in a paracrine mode. We employed a mammalian expression system to synthesize recombinant human KGF and isolated two preparations, KGF-a and KGF-b, from medium conditioned by Chinese hamster ov...

journal_title：Protein expression and purification

authors： Hsu YR,Hsu EW,Katta V,Brankow D,Tseng J,Hu S,Morris CF,Kenney WC,Lu HS

更新日期：1998-03-01 00:00:00

abstract：:Novel GSH-AP (phenoxyl agarose coated gigaporous polystyrene, Agap-co-PSt) microspheres were successfully prepared by introducing GSH ligand into hydrophilic AP microspheres pre-activated with 1,4-butanediol diglycidyl ether. The gigaporous structure and chromatographic properties of GSH-AP medium were evaluated and c...

journal_title：Protein expression and purification

authors： Huang Y,Zhang R,Li J,Li Q,Su Z,Ma G

更新日期：2014-03-01 00:00:00

abstract：:Pseudolysin is the extracellular elastase of Pseudomonas aeruginosa and belongs to the thermolysin-like family of metallopeptidases. Pseudolysin has been identified as a robust drug target and a biotechnologically important enzyme in the tanning industry. Previous attempts to purify active pseudolysin from P. aerugino...

journal_title：Protein expression and purification

authors： Odunuga OO,Adekoya OA,Sylte I

更新日期：2015-09-01 00:00:00

abstract：:The HIV-1 Gag precursor protein, Pr55(Gag), is a multi-domain polyprotein that drives HIV-1 assembly. The morphological features of HIV-1 suggested Pr55(Gag) assumes a variety of different conformations during virion assembly and maturation, yet structural determination of HIV-1 Pr55(Gag) has not been possible due to ...

journal_title：Protein expression and purification

authors： McKinstry WJ,Hijnen M,Tanwar HS,Sparrow LG,Nagarajan S,Pham ST,Mak J

更新日期：2014-08-01 00:00:00

abstract：:This article reviews the development of immobilized-metal affinity chromatography (IMAC) and describes its most important applications. We provide an overview on the use of IMAC in protein fractionation and proteomics, in protein immobilization and detection, and on some special applications such as purification of im...

journal_title：Protein expression and purification

authors： Block H,Maertens B,Spriestersbach A,Brinker N,Kubicek J,Fabis R,Labahn J,Schäfer F

更新日期：2011-09-03 00:00:00

abstract：:Our earlier studies have demonstrated that the 35 kDa isoform of Translocated promoter region protein (Tpr) of Rattus norvegicus was able to augment c-jun transcription efficiently. Identification of direct targets that may in part downregulate c-jun transcription might prove to be an ideal target to curtail the proli...

journal_title：Protein expression and purification

authors： Agarwal S,Yadav SK,Dixit A

更新日期：2011-05-01 00:00:00

abstract：:The gram-positive bacterium Corynebacterium pseudotuberculosis is the causative agent of different diseases that cause dramatically reduced yields of wool and milk, and results in weight loss, carcass condemnation and also death mainly in sheep, equids, cattle and goats and therefore globally results in considerable e...

journal_title：Protein expression and purification

authors： Lindae A,Eberle RJ,Caruso IP,Coronado MA,de Moraes FR,Azevedo V,Arni RK

更新日期：2015-08-01 00:00:00

abstract：:Recombinant cytochrome P450 (CYP or P450) enzymes are useful for drug metabolism research and thereby many expression and purification systems have been developed. Here, we provide a method for the purification of human P450s 3A4 and 1A2 expressed in Escherichia coli using mixed micelles containing anionic phospholipi...

journal_title：Protein expression and purification

authors： Ahn T,Bae CS,Yun CH

更新日期：2014-09-01 00:00:00

abstract：:In Pisum sativum, the short-chain alcohol dehydrogenase-like protein (SAD) gene family consists of at least three members (SAD-A, -B, and -C). Expression of two of these genes (SAD-A and -C) in Escherichia coli or Pichia pastoris resulted in full-length soluble proteins. Purified SAD-A was used as antigen for antibody...

journal_title：Protein expression and purification

authors： Scherbak N,Brosché M,Ala-Häivälä A,Strid H,Ohrfelt A,Nilsson F,Strid A

更新日期：2009-01-01 00:00:00

abstract：:The present report documents an improved enzyme assay method for the mammalian L-alanine:4,5-dioxovalerate transaminase which is of significant utility in work with crude tissue homogenates, cell cultures, or purified enzyme preparations. We also describe a new and rapid purification procedure for this enzyme from rat...

journal_title：Protein expression and purification

authors： Tyagi RK,Datta K

更新日期：1994-12-01 00:00:00

abstract：:Periplasmic expression of recombinant proteins ensures the production of biologically active proteins in a correctly folded state with several key advantages. This research focused on the in-frame cloning of rhIL-15 in pET-20 (+) vector with pelB-leader sequence to direct the protein to the bacterial periplasm. The ta...

journal_title：Protein expression and purification

authors： Ahmed N,Afroze B,Abbas R,Khan MA,Akram M,Tahir S,Bakht S,Munir A,Shahid AA

更新日期：2021-01-01 00:00:00

abstract：:The Human Secretome Project aims to produce and purify all human secreted proteins as full-length. In order to enable this, a robust, gentle and effective purification process is needed, where multiple proteins can be purified in parallel. For this reason, a purification system based on a Protein C-tag and the HPC4 an...

journal_title：Protein expression and purification

authors： Kanje S,Enstedt H,Dannemeyer M,Uhlén M,Hober S,Tegel H

更新日期：2020-11-01 00:00:00

abstract：:The assembly pathway of the insect cell Spodoptera frugiperda (Sf-9) was engineered to include expression of the murine chaperone immunoglobulin heavy chain binding protein (BiP) using the baculovirus vector. The impact of BiP coexpression on the production and secretion of functional and soluble recombinant immunoglo...

journal_title：Protein expression and purification

authors： Hsu TA,Eiden JJ,Bourgarel P,Meo T,Betenbaugh MJ

更新日期：1994-12-01 00:00:00

abstract：:A simple and efficient method for hyperexpression in Escherichia coli and purification of capsid protein, p24, of human immunodeficiency virus type 1 (HIV-1) of both B- and C-subtypes is described. DNA-encoding p24 of C-subtype was cloned from C-subtype gag sequence which was obtained by PCR amplification using DNA ex...

journal_title：Protein expression and purification

authors： Gupta S,Arora K,Gupta A,Chaudhary VK

更新日期：2000-08-01 00:00:00

abstract：:The supply of many valuable proteins that have potential clinical or industrial use is often limited by their low natural availability. With the modern advances in genomics, proteomics and bioinformatics, the number of proteins being produced using recombinant techniques is exponentially increasing and seems to guaran...

journal_title：Protein expression and purification

authors： Papaneophytou CP,Kontopidis G

更新日期：2014-02-01 00:00:00

abstract：:Human granulocyte-macrophage colony-stimulating factor (GM-CSF), a hemopoietic growth factor, was produced and secreted from tobacco cell suspensions. The GM-CSF cDNA was carried by a binary vector under the control of the CaMV 35S promoter and the T7 terminator. In addition, a 5'-nontranslated region from the tobacco...

journal_title：Protein expression and purification

authors： James EA,Wang C,Wang Z,Reeves R,Shin JH,Magnuson NS,Lee JM

更新日期：2000-06-01 00:00:00

abstract：:Nedd4 is an E3 ubiquitin ligase that has received increased attention due to its role in the maintenance of proteostasis and in cellular stress responses. Investigation of Nedd4 enzymology has revealed a complex enzymatic mechanism that involves intermolecular interactions with upstream E2 conjugating enzymes and with...

journal_title：Protein expression and purification

authors： Hatstat AK,McCafferty DG

更新日期：2020-09-01 00:00:00

abstract：:A new method was developed to purify the streptococcal hyaluronate synthase in active form to electrophoretic homogeneity. The method is based on the extraction of protoplast membranes with digitonin and a phase separation into an aqueous and a detergent phase induced by addition of polyethylene glycol 6000 at 0 degre...

journal_title：Protein expression and purification

authors： Prehm S,Nickel V,Prehm P

更新日期：1996-06-01 00:00:00

abstract：:Rat granulocyte macrophage colony-stimulating factor (rGM-CSF) cDNA was amplified and cloned, and recombinant-rGM-CSF (R-rGM-CSF) was expressed and isolated from Escherichia coli. The synthesis of R-rGM-CSF was directed by a modified, inducible maltose binding protein (MBP) gene fusion expression vector, pMTR-23, and ...

journal_title：Protein expression and purification

authors： Holowachuk EW,Ruhoff MS

更新日期：1995-10-01 00:00:00

abstract：:Enteropeptidase (synonym: enterokinase, EC 3.4.21.9) is a heterodimeric serine protease of the intestinal brush border that activates trypsinogen by highly specific cleavage of the trypsinogen activation peptide following the sequence (Asp)(4)-Lys. It has also great biotechnological interest because of the unique subs...

journal_title：Protein expression and purification

authors： Gasparian ME,Bychkov ML,Dolgikh DA,Kirpichnikov MP

更新日期：2011-10-01 00:00:00

abstract：:Plants possess very large numbers of biosynthetic cytochrome P450 enzymes. In spite of the importance of these enzymes for the synthesis of bioactive plant secondary metabolites, only two plant P450 structures has been obtained to date. Isoflavone synthase (IFS) is a membrane-associated cytochrome P450 enzyme catalyzi...

journal_title：Protein expression and purification

authors： Chang Z,Wang X,Wei R,Liu Z,Shan H,Fan G,Hu H

更新日期：2010-12-04 00:00:00

abstract：:Escherichia coli tyrosine kinase (Etk) is a membrane bound kinase in gram-negative bacteria that regulates the export of capsular polysaccharides (CPS). The molecular mechanism behind CPS regulation remains unclear, despite access to a crystal structure of the cytoplasmic kinase domain of Etk. In this study, an effici...

journal_title：Protein expression and purification

authors： Chesterman C,Jia Z

更新日期：2016-09-01 00:00:00

abstract：:The high specific lysyl endopeptidase (Lys-C; EC 3.4.21.50) is often used for the initial fragmentation of polypeptide chains during protein sequence analysis. However, due to its specificity it could be a useful tool for the production of tailor-made protein hydrolysates with for example bioactive or techno functiona...

journal_title：Protein expression and purification

authors： Stressler T,Eisele T,Meyer S,Wangler J,Hug T,Lutz-Wahl S,Fischer L

更新日期：2016-02-01 00:00:00