Abstract:
:Human β-glucuronidase (GUS; EC 3.2.1.31) is a lysosomal enzyme that catalyzes the hydrolysis of β-d-glucuronic acid residues from the non-reducing termini of glycosaminoglycans. Impairment in GUS function leads to the metabolic disorder mucopolysaccharidosis type VII, also known as Sly syndrome. We produced GUS from a CHO cell line grown in suspension in a 15 L perfused bioreactor and developed a three step purification procedure that yields ∼99% pure enzyme with a recovery of more than 40%. The method can be completed in two days and has the potential to be integrated into a continuous manufacturing scheme.
journal_name
Protein Expr Purifjournal_title
Protein expression and purificationauthors
Fratz-Berilla EJ,Ketcham SA,Parhiz H,Ashraf M,Madhavarao CNdoi
10.1016/j.pep.2017.07.011subject
Has Abstractpub_date
2017-12-01 00:00:00pages
28-35eissn
1046-5928issn
1096-0279pii
S1046-5928(17)30134-1journal_volume
140pub_type
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