当前位置： SCI文献检索 > PROTEIN EXPRESSION AND PURIFICATION期刊下所有文献 > Improving purification of recombinant human interferon gamma expressed in Escherichia coli; effect of removal of impurity on the process yield.

Improving purification of recombinant human interferon gamma expressed in Escherichia coli; effect of removal of impurity on the process yield.

Abstract：

:Process development and optimization studies were performed in order to improve the purification process of (rhIFN-gamma). The objective was to generate material with higher purity and quantity. An in-process control screening was developed to obtain the optimal condition for column chromatographic purification by measuring LPS, nucleic acids, rhIFN- gamma, monomer and its covalent dimers. A new resin screening method was applied to select optimal resin for each of the chromatographic columns. The resulting process used Butyl and Q-Sepharose, refolding and SP-Sepharose for purification of IFN-gamma. Effects of different process conditions such as cell lysis, removal of impurity and oxygen concentration were evaluated. Removal of impurities was evaluated by washing of inclusion bodies with 1% Triton X-100 and 3M urea and different chromatography steps. The results reveal that Triton removed about 43% of the LPS but urea had no effect on removal of nucleic acids and LPS. Further analysis show that removal of impurities by column chromatography decreases aggregation and increases the process yield. Oxygen concentration was identified as parameter that could have a significant impact on covalent dimers formation, as an unacceptable pharmaceutical form of rhIFN-gamma. On the basis of small-scale studies, optimum operating conditions were chosen and the purification process was successfully scaled-up to a pilot scale process with step yield and product quality that were better than previous reports.

journal_name

Protein Expr Purif

journal_title

Protein expression and purification

authors

Mohammadian-Mosaabadi J,Naderi-Manesh H,Maghsoudi N,Nassiri-Khalili MA,Masoumian MR,Malek-Sabet N

doi

10.1016/j.pep.2006.07.002

subject

Has Abstract

pub_date

2007-02-01 00:00:00

pages

147-56

issue

2

eissn

1046-5928

issn

1096-0279

pii

S1046-5928(06)00200-2

journal_volume

51

pub_type

杂志文章

相关文献

PROTEIN EXPRESSION AND PURIFICATION文献大全
  • Expression, refolding and purification of Ov-GRN-1, a granulin-like growth factor from the carcinogenic liver fluke, that causes proliferation of mammalian host cells.

    abstract：:Granulins (GRNs) are potent growth factors that are upregulated in many aggressive cancers from a wide range of organs. GRNs form tight, disulphide bonded, beta hairpin stacks, making them difficult to express in recombinant form. We recently described Ov-GRN-1, a GRN family member secreted by the carcinogenic liver f...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2011.06.018

    authors： Smout MJ,Mulvenna JP,Jones MK,Loukas A

    更新日期：2011-10-01 00:00:00

  • Novel self-cleavage activity of Staphylokinase fusion proteins: An interesting finding and its possible applications.

    abstract：:Staphylokinase (SAK) is reported to have a serine protease domain with no proteolytic activity unlike other plasminogen activators like tissue plasminogen activator (t-PA) and urokinase. A unique protease property of Staphylokinase was observed when SAK was expressed as a fusion protein in inducible Escherichia coli e...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2009.07.011

    authors： Prasad B,Salunkhe SS,Padmanabhan S

    更新日期：2010-02-01 00:00:00

  • Improved soluble expression and characterization of the Hc domain of Clostridium botulinum neurotoxin serotype A in Escherichia coli by using a PCR-synthesized gene and a Trx co-expression strain.

    abstract：:Botulinum neurotoxin serotype A (BoNT/A) is an extremely potent bacterial protein toxin. The Hc fragment of BoNT/A (AHc) was shown to be non-toxic, antigenic, and capable of eliciting a protective immunity in animals challenged with homologous BoNT. In this study, we synthesized AHc gene by using T4 DNA ligase and PCR...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2009.11.007

    authors： Chen R,Shi J,Cai K,Tu W,Hou X,Liu H,Xiao L,Wang Q,Tang Y,Wang H

    更新日期：2010-05-01 00:00:00

  • Purification and characterization of the hexokinase from Schistosoma mansoni, expressed in Escherichia coli.

    abstract：:The hexokinase (ATP;D-hexose 6-phosphotransferase, EC 2.7.1.1) of Schistosoma mansoni has been expressed in Escherichia coli as a fusion protein including an N-terminal polyhistidine tag and enterokinase cleavage site. The enzyme was purified by metal chelate chromatography to > 95% homogeneity, based on analysis by S...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1006/prep.1996.0113

    authors： Armstrong RL,Wilson JE,Shoemaker CB

    更新日期：1996-11-01 00:00:00

  • Production of human pancreatic ribonuclease in Saccharomyces cerevisiae and Escherichia coli.

    abstract：:Human pancreatic ribonuclease (HP-RNase) has considerable promise as a therapeutic agent. Structure-function analyses of HP-RNase have been impeded by the difficulty of obtaining the enzyme from its host. Here, a gene encoding HP-RNase was designed, synthesized, and inserted into two expression vectors that then direc...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1006/prep.1996.0036

    authors： Ribó M,delCardayré SB,Raines RT,de Llorens R,Cuchillo CM

    更新日期：1996-05-01 00:00:00

  • Over-expression, purification, and characterization of recombinant NAD-malic enzyme from Escherichia coli K12.

    abstract：:NAD(+)-dependent malic enzyme (NAD-ME) gene from Escherichia coli K12 was inserted into an expression vector pET24b(+) and transformed into E. coli BL21 (DE3). Recombinant NAD-ME was expressed upon IPTG induction, purified with affinity chromatography, and biochemically characterized. The results showed that recombina...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2006.11.017

    authors： Wang J,Tan H,Zhao ZK

    更新日期：2007-05-01 00:00:00

  • Expression of the Trichoderma reesei tyrosinase 2 in Pichia pastoris: isotopic labeling and physicochemical characterization.

    abstract：:Trichoderma reesei tyrosinase TYR2 has been demonstrated to be able to oxidize various phenolic compounds and also peptide and protein bound tyrosine, and thus is of great interest for different biotechnological applications. In order to understand the reaction mechanism of the enzyme it would be essential to solve it...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2007.04.014

    authors： Westerholm-Parvinen A,Selinheimo E,Boer H,Kalkkinen N,Mattinen M,Saloheimo M

    更新日期：2007-09-01 00:00:00

  • Cloning, expression, purification, and characterization of a glutamate-specific endopeptidase from Bacillus licheniformis.

    abstract：:A gene encoding a glutamate-specific endopeptidase (GSE) from Bacillus licheniformis (BL) has been cloned in Escherichia coli cells. The recombinant protein was expressed as cytoplasmic insoluble inclusion bodies. Immobilized metal affinity chromatography was employed to purify the protein, and then a 27-kDa GSE inter...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2011.12.001

    authors： Ye W,Liu J,Wang H,Wang J,Wang X

    更新日期：2012-03-01 00:00:00

  • Expression of soluble and active interferon consensus in SUMO fusion expression system in E. coli.

    abstract：:Protein production can be improved if methods for soluble protein expression are developed. Interferon consensus (IFN-con) is used to treat hepatitis C. IFN-con has superior activity compared to other clinically used interferon α subtypes. However IFN-con is a challenging protein to produce in a soluble form using an ...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2014.03.009

    authors： Peciak K,Tommasi R,Choi JW,Brocchini S,Laurine E

    更新日期：2014-07-01 00:00:00

  • Arabidopsis thaliana plants expressing Rift Valley fever virus antigens: Mice exhibit systemic immune responses as the result of oral administration of the transgenic plants.

    abstract：:The zoonotic Rift Valley fever virus affects livestock and humans in Africa and on the Arabian Peninsula. The economic impact of this pathogen due to livestock losses, as well as its relevance to public health, underscores the importance of developing effective and easily distributed vaccines. Vaccines that can be del...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2016.07.003

    authors： Kalbina I,Lagerqvist N,Moiane B,Ahlm C,Andersson S,Strid Å,Falk KI

    更新日期：2016-11-01 00:00:00

  • A Protein Structure Initiative approach to expression, purification, and in situ delivery of human cytochrome b5 to membrane vesicles.

    abstract：:A specialized vector backbone from the Protein Structure Initiative was used to express full-length human cytochrome b5 as a C-terminal fusion to His8-maltose binding protein in Escherichia coli. The fusion protein could be completely cleaved by tobacco etch virus protease, and a yield of approximately 18 mg of purifi...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2007.11.018

    authors： Sobrado P,Goren MA,James D,Amundson CK,Fox BG

    更新日期：2008-04-01 00:00:00

  • Cloning, expression, isotope labeling, and purification of human antimicrobial peptide LL-37 in Escherichia coli for NMR studies.

    abstract：:Antimicrobial peptide LL-37 plays an important role in human body's first line of defense against infection. To better understand the mechanism of action, it is critical to elucidate the three-dimensional structure of LL-37 in complex with bacterial membranes. We present a bacterial expression system that allows the i...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2005.10.022

    authors： Li Y,Li X,Wang G

    更新日期：2006-06-01 00:00:00

  • Expression and biochemical characterization of a type I methionine aminopeptidase of Plasmodium vivax.

    abstract：:Methionine aminopeptidases (MetAPs), ubiquitous enzymes that play an important role in nascent protein maturation, have been recognized as attractive targets for the development of drugs against pathogenic protozoa including Plasmodium spp. Here, we characterized partial biochemical properties of a type I MetAP of Pla...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2015.01.003

    authors： Kang JM,Ju JW,Kim JY,Ju HL,Lee J,Lee KH,Lee WJ,Sohn WM,Kim TS,Na BK

    更新日期：2015-04-01 00:00:00

  • Expression of a soluble and activatable form of bovine procarboxypeptidase A in Escherichia coli.

    abstract：:Bovine pancreatic procarboxypeptidase A has been overexpressed in a soluble and activatable form in Escherichia coli. When the protein was expressed under the control of bacteriophage T7 promoter in E. coli ADA494 (a thioredoxin reductase deficient bacteria), a thioredoxin fusion protein was produced at relatively hig...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/s1046-5928(02)00573-9

    authors： Seddi R,Chaix JC,Puigserver A,Guo XJ

    更新日期：2003-02-01 00:00:00

  • Purification and characterization of a cold-adapted κ-carrageenase from Pseudoalteromonas sp. ZDY3.

    abstract：:κ-Carrageenase (EC3.2.1.83) is a class of glycoside hydrolase, which can be used for hydrolysis of κ-carrageenan to κ-carrageenan oligosaccharides. In this study, a bacterium, identified as Pseudoalteromonas sp. ZDY3 isolated from rotten algae, was capable to degrade κ-carrageenan. The κ-carrageenase produced by Pseud...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2020.105768

    authors： Zhao D,Jiang B,Zhang Y,Sun W,Pu Z,Bao Y

    更新日期：2021-02-01 00:00:00

  • Expression and characterization of Pen b 26 allergen of Penicillium brevicompactum in Escherichia coli.

    abstract：:Pen b 26 is one of the allergens produced by Penicillium brevicompactum which is one of the most prevalent in door airborne fungi and a major source of respiratory problems, including asthma. Pen b 26 wa scloned and expressed as an N-terminal as well as a C-terminal His6 tagged fusion protein in Escherichia coli. This...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2009.01.004

    authors： Sevinc MS,Kumar V,Abebe M,Mohottalage S,Kumarathasan P,Vincent R,Vijay HM

    更新日期：2009-05-01 00:00:00

  • One-step isolation of alpha 1-acid glycoprotein.

    abstract：:alpha 1-Acid glycoprotein could be isolated by a one-step extraction method from human sera and plasma. Protein recovered in the water phase after extraction with phenol at 70 degrees C for 20 min was verified as human alpha 1-acid glycoprotein when it was compared with the reference standard human alpha 1-acid glycop...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/1046-5928(91)90006-5

    authors： Chan J,Yu D

    更新日期：1991-02-01 00:00:00

  • Purification and characterization of recombinant rabbit cytosolic serine hydroxymethyltransferase.

    abstract：:A rabbit liver cDNA library in phage lambdagt10 was screened using the coding cDNA for human cytosolic serine hydroxymethyltransferase. A clone of 1754 bp was isolated and the nucleotide sequence showed an open reading frame of 1455 bp, which coded for rabbit cytosolic serine hydroxymethyltransferase and was flanked b...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1006/prep.1998.0890

    authors： di Salvo ML,Delle Fratte S,De Biase D,Bossa F,Schirch V

    更新日期：1998-07-01 00:00:00

  • Expression in Trichoderma reesei and characterisation of a thermostable family 3 beta-glucosidase from the moderately thermophilic fungus Talaromyces emersonii.

    abstract：:The gene encoding a thermostable beta-glucosidase (cel3a) was isolated from the thermophilic fungus Talalaromyces emersonii by degenerate PCR and expressed in the filamentous fungus Trichoderma reesei. The cel3a gene encodes an 857 amino acid long protein with a calculated molecular weight of 90.59 kDa. Tal. emersonii...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2004.08.006

    authors： Murray P,Aro N,Collins C,Grassick A,Penttilä M,Saloheimo M,Tuohy M

    更新日期：2004-12-01 00:00:00

  • Delandin, a chitinase-like protein with antifungal, HIV-1 reverse transcriptase inhibitory and mitogenic activities from the rice bean Delandia umbellata.

    abstract：:An antifungal protein with a chitinase-like N-terminal sequence, designated delandin, was isolated from the rice bean. The protein exhibited a molecular weight of 28 kDa and was adsorbed on both blue Affi-Gel and SP-Toyopearl. It exerted antifungal action toward Mycosphaerella arachidicola, Botrytis cinerea, Fu- sariu...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1006/prep.2001.1596

    authors： Ye XY,Ng TB

    更新日期：2002-04-01 00:00:00

  • Zebrafish Sp1-like protein is structurally and functionally comparable to human Sp1.

    abstract：:The transcription factor Sp1 is a regulator of TATA-less genes. It belongs to the Cys₂-His₂ zinc finger domain-containing family. A zebrafish cDNA encoding a peptide homologous to mammalian Sp1 was cloned and inserted into a pET43.1a vector and expressed in Escherichia coli Rosetta (DE3) cells as a Nus-His-tag fusion ...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2010.10.010

    authors： Lin CJ,Hsiao TH,Chung YS,Chang WN,Yeh TM,Chen BH,Fu TF

    更新日期：2011-03-01 00:00:00

  • Improved expression and characterization of Ca2+-ATPase and phospholamban in High-Five cells.

    abstract：:The Ca2+-ATPase accounts for the majority of Ca2+ removed from the cytoplasm during cardiac muscle relaxation. The Ca2+-ATPase is regulated by phospholamban, a 52 amino acid phosphoprotein, which inhibits Ca2+-ATPase activity by decreasing the apparent affinity of the ATPase for Ca2+. To study the physical mechanism o...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2003.11.005

    authors： Waggoner JR,Huffman J,Griffith BN,Jones LR,Mahaney JE

    更新日期：2004-03-01 00:00:00

  • Affinity purification of protein complexes for analysis by multidimensional protein identification technology.

    abstract：:Characterizing protein complexes and identifying their subunits promote our understanding of the machinery involved in many in vivo processes. Proteomic studies can identify a protein's binding partners, and this can provide insight into how protein complexes function and how they are regulated. In addition, the compo...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2012.09.007

    authors： Banks CA,Kong SE,Washburn MP

    更新日期：2012-12-01 00:00:00

  • A new protocol for high-yield purification of recombinant human CXCL8((3-72))K11R/G31P expressed in Escherichia coli.

    abstract：:The ELR-CXC chemokines are important to neutrophil inflammation in many acute and chronic diseases. Among them, CXCL8 (interleukin-8, IL-8), binds to both the CXCR1 and CXCR2 receptors with high affinity and the expression levels of CXCL8 are elevated in many inflammatory diseases. Recently, an analogue of human CXCL8...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2008.04.007

    authors： Cheng HT,Huang KC,Yu HY,Gao KJ,Zhao X,Li F,Town J,Gordon JR,Cheng JW

    更新日期：2008-09-01 00:00:00

  • Binding studies using Pichia pastoris expressed human aryl hydrocarbon receptor and aryl hydrocarbon receptor nuclear translocator proteins.

    abstract：:The aryl hydrocarbon receptor (AHR) is a transcription factor which activates gene transcription by binding to its corresponding enhancer as the heterodimer, which is consisted of AHR and the aryl hydrocarbon receptor nuclear translocator (ARNT). Human AHR can be rather difficult to study, when compared among the AHR ...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2016.02.011

    authors： Zheng Y,Xie J,Huang X,Dong J,Park MS,Chan WK

    更新日期：2016-06-01 00:00:00

  • Biotechnological applications of elastin-like polypeptides and the inverse transition cycle in the pharmaceutical industry.

    abstract：:Proteins are essential throughout the biological and biomedical sciences and the purification strategies of proteins of interest have advanced over centuries. Elastin-like polypeptides (ELPs) are compound polymers that have recently been highlighted for their sharp and reversible phase transition property when heated ...

    journal_title：Protein expression and purification

    pub_type： 杂志文章,评审

    doi：10.1016/j.pep.2018.09.006

    authors： Fletcher EE,Yan D,Kosiba AA,Zhou Y,Shi H

    更新日期：2019-01-01 00:00:00

  • Remedial strategies in structural proteomics: expression, purification, and crystallization of the Vav1/Rac1 complex.

    abstract：:The signal transduction pathway involving the Vav1 guanine nucleotide exchange factor (GEF) and the Rac1 GTPase plays several key roles in the immune response mediated by the T cell receptor. Vav1 is also a unique member of the GEF family in that it contains a cysteine-rich domain (CRD) that is critical for Rac1 bindi...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2006.10.027

    authors： Brooun A,Foster SA,Chrencik JE,Chien EY,Kolatkar AR,Streiff M,Ramage P,Widmer H,Weckbecker G,Kuhn P

    更新日期：2007-05-01 00:00:00

  • Large-scale expression and purification of a soluble form of the pleckstrin homology domain of the human protooncogenic serine/threonine protein kinase PKB (c-akt) in Escherichia coli.

    abstract：:The protooncogenic serine/threonine protein kinase PKB contains an amino-terminal pleckstrin homology (PH) domain which binds phosphatidylinositides. The PH domain, composed of approximately 100 loosely conserved amino acids, is found in many proteins, including kinases, phospholipases C, GTPases, GTPase-activating pr...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1006/prep.1999.1120

    authors： Ingley E,Hemmings BA

    更新日期：1999-11-01 00:00:00

  • Rapid purification of recombinant betaB2-crystallin using hydrophobic interaction chromatography.

    abstract：:BetaB2-crystallin, the major subunit of beta-crystallins, is difficult to purify either from lens homogenate or from betaH-or betaL-crystallins. It has been prepared by heterologous expression in Escherichia coli. Most often, the methods used for purifying a recombinant globular protein employ the combination of ion-e...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/s1046-5928(02)00675-7

    authors： Jobby MK,Sharma Y

    更新日期：2003-03-01 00:00:00

  • Heterologous expression, purification, and characterization of human triacylglycerol hydrolase.

    abstract：:Triacylglycerol hydrolase mobilizes stored triacylglycerol some of which is used for very-low-density lipoprotein assembly in the liver. A full-length cDNA coding for a human triacylglycerol hydrolase (hTGH) was isolated from a human liver cDNA library. The cDNA has an open reading frame of 576 amino acids with a clea...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1006/prep.2001.1553

    authors： Alam M,Ho S,Vance DE,Lehner R

    更新日期：2002-02-01 00:00:00