Delandin, a chitinase-like protein with antifungal, HIV-1 reverse transcriptase inhibitory and mitogenic activities from the rice bean Delandia umbellata.

abstract：:A recombinant H(C) fragment of botulinum neurotoxin, serotype A (rBoNTA(H(C))), has been successfully expressed in a Mut(+) strain of the methylotrophic yeast Pichia pastoris for use as an antigen in a proposed human vaccine. Fermentation employed glycerol batch, glycerol-fed batch, and methanol-fed batch phases to ac...

journal_title：Protein expression and purification

authors： Potter KJ,Zhang W,Smith LA,Meagher MM

更新日期：2000-08-01 00:00:00

abstract：:A fairly rapid and improved method for producing large amounts of highly pure apoaequorin, the apoprotein of aequorin which emits light on binding Ca2+, is described. The method consists of fusing the gene of the outer membrane protein A (ompA) secretion signal peptide of Escherichia coli to the apoaequorin gene and e...

journal_title：Protein expression and purification

authors： Inouye S,Zenno S,Sakaki Y,Tsuji FI

更新日期：1991-04-01 00:00:00

abstract：:The coding region for arginyl-tRNA synthetase from jack bean (Canavalia ensiformis) has been sequenced and cloned into the bacterial expression vector pET32a. Transformation of BL21 cells and induction with IPTG results in the high level expression of the protein fused N-terminally with thioredoxin and bearing a His-t...

journal_title：Protein expression and purification

authors： Hogg J,Schiefermayr E,Schiltz E,Igloi GL

更新日期：2008-10-01 00:00:00

abstract：:Arginase (EC 3.5.3.1; L-arginine amidinohydrolase) is a key enzyme of the urea cycle that catalyses the conversion of arginine to ornithine and urea, which is the final cytosolic reaction of urea formation in the mammalian liver. The recombinant strain of the yeast Saccharomyces cerevisiae that is capable of overprodu...

journal_title：Protein expression and purification

authors： Zakalskiy AE,Zakalska OM,Rzhepetskyy YA,Potocka N,Stasyk OV,Horak D,Gonchar MV

更新日期：2012-01-01 00:00:00

abstract：:Magnolia officinalis Rehder et Wilson is a traditional Chinese herbal medicine that is used to treat various diseases such as neurosis, anxiety, and stroke. The main secondary metabolites in magnolia bark are phenolic compounds and terpenoids. Squalene synthase plays a significant role in catalyzing two farnesyl dipho...

journal_title：Protein expression and purification

authors： Zha L,Liu S,Su P,Yuan Y,Huang L

更新日期：2016-04-01 00:00:00

abstract：:Escherichia coli cells were transformed with an expression vector constructed by inserting a DNA fragment encoding a Kazal-type trypsin inhibitor from mouse seminal vesicle into pGEX-2. The cloned cells were able to produce a high yield of a chimeric polypeptide made by fusing the trypsin inhibitor to glutathione S-tr...

journal_title：Protein expression and purification

authors： Lai ML,Li SH,Chen YH

更新日期：1994-02-01 00:00:00

abstract：:Phenylalanine hydroxylase, important in phenylalanine metabolism in mammals, is regulated through short-term (activation) and long-term (induction) mechanisms. To help elucidate the structure-function relationships involved in the activation of this enzyme, we have isolated and characterized full-length cDNA clones to...

journal_title：Protein expression and purification

authors： Citron BA,Davis MD,Kaufman S

更新日期：1992-04-01 00:00:00

abstract：:Separation of copurifying protease activity from recombinant human Müllerian inhibiting substance (rhMIS) bound to a monoclonal antibody immunoaffinity column by a high-salt wash results in cleaner preparations of rhMIS resistant to cleavage upon storage. In addition, an inhibitor of rhMIS antiproliferative activity i...

journal_title：Protein expression and purification

authors： Ragin RC,Donahoe PK,Kenneally MK,Ahmad MF,MacLaughlin DT

更新日期：1992-06-01 00:00:00

abstract：:Chan Su is a traditional medicine prepared from toxic secretions from the auricular and skin glands of Chinese toads. Previous studies show that active components in Chan Su can inhibit the proliferation of tumor cells. To study the effect of Chan Su peptides on angiogenesis, fresh Chan Su was collected and its compon...

journal_title：Protein expression and purification

authors： Xia F,Gao F,Yao H,Zhang G,Gao B,Lu Y,Wang X,Qian Y

更新日期：2019-11-01 00:00:00

abstract：:The covalent addition of 4-amino-4-deoxy-L-arabinose (L-Ara4N) groups to lipid A, which resides in the outer membranes of bacteria such as Salmonella typhimurium and Escherichia coli, is the final step in the polymyxin-resistance pathway in these organisms. This modification is catalyzed by the inner membrane protein ...

journal_title：Protein expression and purification

authors： Bretscher LE,Morrell MT,Funk AL,Klug CS

更新日期：2006-03-01 00:00:00

abstract：:In order to produce sufficient quantities of fibroblast growth factor-saporin (rFGF-2-SAP) mitotoxin for preclinical evaluation in models of diseases such as cancer and restenosis, we have undertaken the large-scale expression, purification, and characterization of the recombinant molecule. The fusion gene encoding rF...

journal_title：Protein expression and purification

authors： McDonald JR,Ong M,Shen C,Parandoosh Z,Sosnowski B,Bussell S,Houston LL

更新日期：1996-08-01 00:00:00

abstract：:The sequence and structure of the target protein exert a marked effect on its soluble expression in Escherichia coli. The effects of the mutation of an amylase isolated from Bacillus licheniformis (BLA) on its soluble expression in E. coli were investigated. A random mutation library of BLA was constructed to screen f...

journal_title：Protein expression and purification

authors： Wang P,Qin W,Xu J,Yan Y,Tian J,Wu N,Yao B

更新日期：2016-04-01 00:00:00

abstract：:Pseudolysin is the extracellular elastase of Pseudomonas aeruginosa and belongs to the thermolysin-like family of metallopeptidases. Pseudolysin has been identified as a robust drug target and a biotechnologically important enzyme in the tanning industry. Previous attempts to purify active pseudolysin from P. aerugino...

journal_title：Protein expression and purification

authors： Odunuga OO,Adekoya OA,Sylte I

更新日期：2015-09-01 00:00:00

abstract：:The aim of this study was to evaluate the parameters that affect the production of the recombinant 10 kDa culture filtrate protein (CFP10), a promising reagent of high specificity for intradermoreaction and other antigen-based methods used in the diagnosis of tuberculosis. Conditions of Escherichia coli growth tempera...

journal_title：Protein expression and purification

authors： Dela Coletta Troiano Araújo L,Wibrantz M,Rodríguez-Fernández DE,Karp SG,Talevi AC,Maltempi de Souza E,Soccol CR,Thomaz-Soccol V

更新日期：2019-02-01 00:00:00

abstract：:S-locus protein kinase (SRK) is a receptor kinase that plays a critical role in self-recognition in the Brassicaceae self-incompatibility (SI) response. SRK is activated by binding of its ligand S-locus protein 11 (SP11) and subsequently induced phosphorylation of the intracellular kinase domain. However, a detailed a...

journal_title：Protein expression and purification

authors： Murase K,Hirano Y,Takayama S,Hakoshima T

更新日期：2017-03-01 00:00:00

abstract：:l-glutamate oxidase (GLOD), encoded by the gox gene, catalyses the transformation of l-glutamic acid into α-ketoglutaric acid (α-KG). In the present study, Pichia pastoris was used for heterologous production of GLOD following optimization of the gox coding sequence for expression in the yeast host. A series of constr...

journal_title：Protein expression and purification

authors： YaPing W,Ben R,Hong Y,Rui H,Li L,Ping'an L,Lixin M

更新日期：2017-01-01 00:00:00

abstract：:Autophagy is the process of degradation of intracellular proteins through the lysosome. Members of the tripartite motif (TRIM) proteins have shown to directly recognize autophagic cargo and also to act as a hub for the phagophore nucleation complex. The TRIM proteins are classically characterized by the presence of an...

journal_title：Protein expression and purification

authors： Guimarães DS,Gomes MD

更新日期：2018-03-01 00:00:00

abstract：:The human melanocortin 4 receptor (MC4r) was successfully expressed in Sf9 cells using the baculovirus infection system. N- and C-terminally His-tagged receptors generated B(max) values of 14 and 23 pmol receptor/mg membrane protein, respectively. The highest expression level obtained with the C-terminally His-tagged ...

journal_title：Protein expression and purification

authors： Dolby V,Collén A,Lundqvist A,Cronet P

更新日期：2004-10-01 00:00:00

abstract：:Fatty acid desaturases are enzymes that introduce double bonds into fatty acyl chains, among which stearoyl-acyl carrier protein desaturase (S-ACP-DES) was widely distributed in the plant kingdom. We cloned the cDNA coding for fab2/ssi2, an S-ACP-DES from Arabidopsis thaliana, into the vector pET30a and heterologously...

journal_title：Protein expression and purification

authors： Cao Y,Xian M,Yang J,Xu X,Liu W,Li L

更新日期：2010-02-01 00:00:00

abstract：:Molecular chaperones are integral components of the cellular machinery involved in ensuring correct protein folding and the continued maintenance of protein structure. An understanding of these ubiquitous molecules is key to finding cures to protein misfolding diseases such as Alzheimer's and Creutzfeldt-Jacob disease...

journal_title：Protein expression and purification

authors： Nicoll WS,Boshoff A,Ludewig MH,Hennessy F,Jung M,Blatch GL

更新日期：2006-03-01 00:00:00

abstract：:Human HEK293 cells that stably express the Epstein Barr nuclear antigen 1 (EBNA1) support the episomal replication of plasmids containing the Epstein Barr virus origin of replication (EBV oriP). A 293EBNA (293E) cell line expressing the human corticotropin-releasing hormone receptor subtype I (CRHR1) from an episomal ...

journal_title：Protein expression and purification

authors： Horlick RA,Sperle K,Breth LA,Reid CC,Shen ES,Robbins AK,Cooke GM,Largent BL

更新日期：1997-04-01 00:00:00

abstract：:Organophosphorus hydrolase (OPH) is a ∼38kDa enzyme encoded by opd gene of Flavobacterium sp. The enzyme can hydrolyze and inactivate variety of organophosphate (OP)-compounds, including chemical warfare nerve agents. Thus, OPH is a strong candidate for the development of therapeutic intervention against OP-poisoning ...

journal_title：Protein expression and purification

authors： Satvik Iyengar AR,Tripathy RK,Bajaj P,Pande AH

更新日期：2015-07-01 00:00:00

abstract：:Production of antibody fragments in heterologous hosts such as Escherichiacoli provides a unique and cost-effective method to develop engineered vectors for tumor targeting. A single-chain Fragment variable (scFv) of the murine monoclonal antibody MAb-B43.13 targeting CA125 in epithelial ovarian cancer was previously ...

journal_title：Protein expression and purification

authors： Sharma SK,Suresh MR,Wuest FR

更新日期：2014-10-01 00:00:00

abstract：:The recombinant truncated endolysin LysK consisting of two catalytic domains, N-terminal CHAP and amidase-2 (LysKCA) was overexpressed in E. coli in the form of inclusion bodies (IBs). These IBs were dissolved in 6 M solution of urea followed by the refolding process. The refolding efficacy of the dilution and matrix-...

journal_title：Protein expression and purification

authors： Alaksandr Ž,Sergey G,Maksim P,Sergey K,Niyaz S,Uladzimir P,Mikhail S

更新日期：2020-10-01 00:00:00

abstract：:The erm proteins confer resistance to the MLS (macrolide-lincosamide-streptogramin B) antibiotics in various microorganisms, including pathogens, through dimethylation of a single adenine residue (A2085: Bacillus subtilis coordinate) of the 23S rRNA to reduce the affinity of antibiotics, thereby enabling the cells to ...

journal_title：Protein expression and purification

authors： Jin HJ,Yang YD

更新日期：2002-06-01 00:00:00

abstract：:Receptor tyrosine kinase like orphan receptor 2 (ROR2) is a co-receptor for some Wnt proteins including Wnt5a that activate the noncanonical Wnt/planar cell polarity (PCP) signaling pathway. Upregulation of ROR2 is associated with several cancer forms. The extracellular region of ROR2, which contains an immunoglobulin...

journal_title：Protein expression and purification

authors： Li Y,Han X,Xu W,Rao Z,Li X

更新日期：2019-06-01 00:00:00

abstract：:The cDNA for the alpha i1 protein that had undergone site-directed mutagenesis to change glycine-2 to alanine was ligated into a baculovirus transfer vector. A recombinant virus was obtained by transfecting Sf9 cells with both the wild-type baculovirus DNA and the transfer vector and screening for recombinant plaques....

journal_title：Protein expression and purification

authors： Jones TL,Woodard C,Spiegel AM

更新日期：1993-02-01 00:00:00

abstract：:A method for purifying acylation stimulating protein (ASP) from porcine serum is described. The mRNA encoding ASP was cloned by reverse transcriptase-polymerase chain reaction which predicted a 76 residue peptide. Based on this sequence, we generated antisera to a C-terminal peptide (ASP(1-20)) which aided ASP purific...

journal_title：Protein expression and purification

authors： Zhang H,Jacobi SK,Toombs CF,Cianflone KH,Nersesian N,Sarath G,Miner JL

更新日期：2002-07-01 00:00:00

abstract：:The production of a single-chain variable fragment (scFv) antibody against bovine ribonuclease A in the cytoplasm of Escherichia coli trxB/gor double mutant was investigated. Previous reports have shown that the thioredoxin (Trx) protein fusion strategy is useful for the correct folding of scFvs and that the expressio...

journal_title：Protein expression and purification

authors： Sonoda H,Kumada Y,Katsuda T,Yamaji H

更新日期：2010-04-01 00:00:00

abstract：:The transcription factor Sp1 is a regulator of TATA-less genes. It belongs to the Cys₂-His₂ zinc finger domain-containing family. A zebrafish cDNA encoding a peptide homologous to mammalian Sp1 was cloned and inserted into a pET43.1a vector and expressed in Escherichia coli Rosetta (DE3) cells as a Nus-His-tag fusion ...

journal_title：Protein expression and purification

authors： Lin CJ,Hsiao TH,Chung YS,Chang WN,Yeh TM,Chen BH,Fu TF

更新日期：2011-03-01 00:00:00