Abstract:
:A method for purifying acylation stimulating protein (ASP) from porcine serum is described. The mRNA encoding ASP was cloned by reverse transcriptase-polymerase chain reaction which predicted a 76 residue peptide. Based on this sequence, we generated antisera to a C-terminal peptide (ASP(1-20)) which aided ASP purification. Identity of the purified protein was verified by N-terminal sequencing. The molecular mass of porcine ASP is 8926. Porcine ASP stimulated esterification of fatty acid into triacylglycerol in cultured human cells with potency similar to that of human ASP (twofold at 5 microM). Based on this evidence that ASP exists in porcine blood, and that it has acylation stimulating activity, we propose that ASP may play a role in regulation of energy storage in adipose tissue in the pig.
journal_name
Protein Expr Purifjournal_title
Protein expression and purificationauthors
Zhang H,Jacobi SK,Toombs CF,Cianflone KH,Nersesian N,Sarath G,Miner JLdoi
10.1016/s1046-5928(02)00019-0keywords:
subject
Has Abstractpub_date
2002-07-01 00:00:00pages
348-52issue
2eissn
1046-5928issn
1096-0279pii
S1046592802000190journal_volume
25pub_type
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journal_title:Protein expression and purification
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journal_title:Protein expression and purification
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journal_title:Protein expression and purification
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journal_title:Protein expression and purification
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journal_title:Protein expression and purification
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