Expression and properties of arginyl-tRNA synthetase from jack bean (Canavalia ensiformis).

abstract：:The sequence of the gene from Nocardia sp. NRRL 5646 encoding GTP cyclohydrolase I (GCH), gch, and its adjacent regions was determined. The open reading frame of Nocardia gch contains 684 nucleotides, and the deduced amino acid sequence represents a protein of 227 amino acid residues with a calculated molecular mass o...

journal_title：Protein expression and purification

authors： He A,Simpson DR,Daniels L,Rosazza JP

更新日期：2004-06-01 00:00:00

abstract：:The gram-positive bacterium Corynebacterium pseudotuberculosis is the causative agent of different diseases that cause dramatically reduced yields of wool and milk, and results in weight loss, carcass condemnation and also death mainly in sheep, equids, cattle and goats and therefore globally results in considerable e...

journal_title：Protein expression and purification

authors： Lindae A,Eberle RJ,Caruso IP,Coronado MA,de Moraes FR,Azevedo V,Arni RK

更新日期：2015-08-01 00:00:00

abstract：:DNA gyrase is a type IIA topoisomerase found in bacteria but not in humans. The enzyme is required for bacterial DNA replication and transcription, and is an important antibacterial target that is sensitive to the widely-used fluoroquinolone drugs. Due to the emergence of fluoroquinolone resistance, the discovery of n...

journal_title：Protein expression and purification

authors： Aung HL,Samaranayaka CU,Enright R,Beggs KT,Monk BC

更新日期：2015-03-01 00:00:00

abstract：:κ-Carrageenase (EC3.2.1.83) is a class of glycoside hydrolase, which can be used for hydrolysis of κ-carrageenan to κ-carrageenan oligosaccharides. In this study, a bacterium, identified as Pseudoalteromonas sp. ZDY3 isolated from rotten algae, was capable to degrade κ-carrageenan. The κ-carrageenase produced by Pseud...

journal_title：Protein expression and purification

authors： Zhao D,Jiang B,Zhang Y,Sun W,Pu Z,Bao Y

更新日期：2021-02-01 00:00:00

abstract：:(S)-Hydroxynitrile lyase (Hnl) from the tropical rubber tree Hevea brasiliensis catalyzes the formation of (S)-cyanohydrins from hydrocyanic acid and aldehydes or ketones. This enzyme accepts aliphatic, aromatic, and heterocyclic carbonyl compounds as substrates and is therefore considered a potent biocatalyst for the...

journal_title：Protein expression and purification

authors： Hasslacher M,Schall M,Hayn M,Bona R,Rumbold K,Lückl J,Griengl H,Kohlwein SD,Schwab H

更新日期：1997-10-01 00:00:00

abstract：:Plantaricin JK (PlnJK) is a Class IIb LAB bacteriocin that includes two peptides; i.e., PlnJ and PlnK, which can synergistically halt many types of gram-positive bacteria, including food spoilage organisms. Purification of these peptides from natural lactic acid bacteria is difficult therefore, their application remai...

journal_title：Protein expression and purification

authors： Xu Y,Yang L,Li P,Gu Q

更新日期：2019-07-01 00:00:00

abstract：:Heparinase I (HepA) was originally isolated from Flavobacterium heparinum (F. heparinum) and specifically cleaves heparin/heparan sulfate in a site-dependent manner, showing great promise for producing low molecular weight heparin (LMWH). However, expressing recombinant HepA is extremely difficult in Escherichia coli ...

journal_title：Protein expression and purification

authors： Huang J,Cao L,Guo W,Yuan R,Jia Z,Huang K

更新日期：2012-06-01 00:00:00

abstract：:Streptococcal protein G has been used extensively for the purification of antibodies using the interaction of the Fc region with protein G. Many antibodies also interact with protein G through a low-affinity binding site for the Fab region. The exploitation of this low-affinity interaction for the purification of Fab'...

journal_title：Protein expression and purification

authors： Proudfoot KA,Torrance C,Lawson AD,King DJ

更新日期：1992-10-01 00:00:00

abstract：:Rat granulocyte macrophage colony-stimulating factor (rGM-CSF) cDNA was amplified and cloned, and recombinant-rGM-CSF (R-rGM-CSF) was expressed and isolated from Escherichia coli. The synthesis of R-rGM-CSF was directed by a modified, inducible maltose binding protein (MBP) gene fusion expression vector, pMTR-23, and ...

journal_title：Protein expression and purification

authors： Holowachuk EW,Ruhoff MS

更新日期：1995-10-01 00:00:00

abstract：:We have used Ni(2+)-affinity chromatography as a rapid and efficient method to purify a sensory rhodopsin I (SR-I) derivative containing six consecutive histidine residues at its C-terminus (His-tagged SR-I). The protein was expressed in Halobacterium salinarium by integrating the corresponding gene at the chromosomal...

journal_title：Protein expression and purification

authors： Krebs MP,Spudich EN,Spudich JL

更新日期：1995-12-01 00:00:00

abstract：:Expression of recombinant proteins is an important step towards elucidating the functions of many genes discovered through genomic sequencing projects. It is also critical for validating gene targets and for developing effective therapies for many diseases. Here we describe a novel method to express recombinant protei...

journal_title：Protein expression and purification

authors： Wang A,Clapper J,Guderian JA,Foy TM,Fanger GR,Retter MW,Skeiky YA

更新日期：2003-07-01 00:00:00

abstract：:A cDNA clone, lambda GTHP1del, encoding glutathione transferase (GST) P1-1, was isolated from a human K562 erythroleukemia cell line cDNA library. The coding sequence was lacking the codons for the N-terminal 34 amino acids. A DNA segment was designed in order to obtain the missing portion and a structure representing...

journal_title：Protein expression and purification

authors： Kolm RH,Stenberg G,Widersten M,Mannervik B

更新日期：1995-06-01 00:00:00

abstract：:HtrA2 is an apoptosis-activating protein to enhance the apoptotic process by preventing the formation of the IAP-caspase complex, thus freeing caspase to trigger the apoptosis pathway. Here, we presented the full-length sequence of HtrA2 from the black tiger shrimp (PmHtrA2). The full-length PmHtrA2 transcript was 140...

journal_title：Protein expression and purification

authors： Suwannaboon R,Phiwsaiya K,Senapin S,Khunrae P,Rattanarojpong T

更新日期：2013-12-01 00:00:00

abstract：:Staphylokinase (SAK) is reported to have a serine protease domain with no proteolytic activity unlike other plasminogen activators like tissue plasminogen activator (t-PA) and urokinase. A unique protease property of Staphylokinase was observed when SAK was expressed as a fusion protein in inducible Escherichia coli e...

journal_title：Protein expression and purification

authors： Prasad B,Salunkhe SS,Padmanabhan S

更新日期：2010-02-01 00:00:00

abstract：:A full-length cDNA sequence of plant type CRY (designated Hae-P-CRY) was cloned from the green alga Haematococcus pluvialis. The cDNA sequence was 3608 base pairs (bp) in length, which contained a 2988-bp open reading frame encoding 995 amino acids with molecular mass of 107.7 kDa and isoelectric point of 6.19. Multip...

journal_title：Protein expression and purification

authors： Hang W,Gujar A,Zhang H,Xu W,Zhao C,Zhu X,Xue J,Zhang C,Ji C,Qin S,Li R,Cui H

更新日期：2020-08-01 00:00:00

abstract：:For affinity-chromatography-based purification of proteins that are prone to abnormal termination of translation or that may not be modified at their N-termini, affinity tags are needed which can be fused to the C-terminus. In this publication we describe that maltose binding protein (MBP) fused to the C-terminus of t...

journal_title：Protein expression and purification

authors： Hennig L,Schäfer E

更新日期：1998-12-01 00:00:00

abstract：:Huperzine A (HupA) is a drug used for the treatment of Alzheimer's disease. However, the biosynthesis of this medicinally important compound is not well understood. The HupA biosynthetic pathway is thought to be initiated by the decarboxylation of lysine to form cadaverine, which is then converted to 5-aminopentanal b...

journal_title：Protein expression and purification

authors： Yang H,Peng S,Zhang Z,Yan R,Wang Y,Zhan J,Zhu D

更新日期：2016-12-01 00:00:00

abstract：:Enzymatic hydrolysis of the N-iminylamide was investigated in this study. An enzyme possessing N-iminylamidase activity from pig liver was purified to electrophoretic homogeneity. This enzyme was also active, however, with imides and appears to be identical to pig liver imidase. The identification was confirmed by cop...

journal_title：Protein expression and purification

authors： Huang CY,Yang YS

更新日期：2005-03-01 00:00:00

abstract：:Adrenal cytochrome b561 (cyt b561) is the prototypical member of an emerging family of proteins that are distributed widely in vertebrate, invertebrate and plant tissues. The adrenal cytochrome is an integral membrane protein with two b-type hemes and six predicted transmembrane helices. Adrenal cyt b561 is involved i...

journal_title：Protein expression and purification

authors： Liu W,Rogge CE,Kamensky Y,Tsai AL,Kulmacz RJ

更新日期：2007-12-01 00:00:00

abstract：:Darbepoetin alfa is an engineered and hyperglycosylated analog of recombinant human erythropoietin (EPO) which is used as a drug in treating anemia in patients with chronic kidney failure and cancer. This study desribes the secretory expression of a codon-optimized recombinant form of darbepoetin alfa in Leishmania ta...

journal_title：Protein expression and purification

authors： Kianmehr A,Golavar R,Rouintan M,Mahrooz A,Fard-Esfahani P,Oladnabi M,Khajeniazi S,Mostafavi SS,Omidinia E

更新日期：2016-02-01 00:00:00

abstract：:Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), which is capable of selectively inducing apoptosis of cancer cells, is a potential targeted drug for cancer therapy. The TRAIL protein induces apoptosis only in trimeric form. However, the recombinant soluble TRAIL (sTRAIL) trimer has low stability and a...

journal_title：Protein expression and purification

authors： Wang B,Wang Z,Yan J,Wang L,Wang Z,Wu J,Zhang H,Wu H,Kong W,Yu B,Yu X

更新日期：2015-11-01 00:00:00

abstract：:Kynurenine 3-monooxygenase (KMO) is an enzyme central to the kynurenine pathway of tryptophan metabolism. KMO has been implicated as a therapeutic target in several disease states, including Huntington's disease. Recombinant human KMO protein production is challenging due to the presence of transmembrane domains, whic...

journal_title：Protein expression and purification

authors： Wilson K,Mole DJ,Binnie M,Homer NZ,Zheng X,Yard BA,Iredale JP,Auer M,Webster SP

更新日期：2014-03-01 00:00:00

abstract：:The superoxide dismutases (EC 1.15.1.1) are a family of enzymes that catalyze the dismutation of superoxide radical anion to dioxygen and hydrogen peroxide. The active site contains a critical metal ion such as manganese, iron, or copper. The copper-containing protein also has one zinc ion bound per subunit. The stand...

journal_title：Protein expression and purification

authors： Sutter B,Bounds PL,Koppenol WH

更新日期：2000-06-01 00:00:00

abstract：:Antibacterial peptides from various sources express different antibacterial activity. In order to obtain a high activity antibacterial peptide, the sequences of four antimicrobial peptides--Protegrin-1, 4 kDa Scorpion Defensin, Metalnikowin-2A and Sheep Myeloid Antibacterial Peptide SMAP-29--were exploited to generate...

journal_title：Protein expression and purification

authors： Niu M,Li X,Wei J,Cao R,Zhou B,Chen P

更新日期：2008-01-01 00:00:00

abstract：:Periplasmic expression of recombinant proteins ensures the production of biologically active proteins in a correctly folded state with several key advantages. This research focused on the in-frame cloning of rhIL-15 in pET-20 (+) vector with pelB-leader sequence to direct the protein to the bacterial periplasm. The ta...

journal_title：Protein expression and purification

authors： Ahmed N,Afroze B,Abbas R,Khan MA,Akram M,Tahir S,Bakht S,Munir A,Shahid AA

更新日期：2021-01-01 00:00:00

abstract：:We have investigated the suitability of Pichia pastoris as an expression system for the candidate therapeutic protein, Sonic hedgehog fused to an immunoglobulin Fc domain (Shh-Fc). Sonic hedgehog is a morphogen protein involved in the patterning of a wide range of tissues during animal embryogenesis. The presence of S...

journal_title：Protein expression and purification

authors： Shapiro RI,Wen D,Levesque M,Hronowski X,Gill A,Garber EA,Galdes A,Strauch KL,Taylor FR

更新日期：2003-06-01 00:00:00

abstract：:Mitochondrial 2,4-dienoyl-CoA reductase is a key enzyme for the beta-oxidation of unsaturated fatty acids. The cDNA of the full-length human mitochondrial 2,4-dienoyl-CoA reductase was previously cloned as pUC18::DECR. PCR methodologies were used to subclone the genes encoding various truncated human mitochondrial 2,4...

journal_title：Protein expression and purification

authors： Chu X,Yu W,Chen G,Li D

更新日期：2003-10-01 00:00:00

abstract：:Arginine has been effectively used in various column chromatographies for improving recovery and resolution, and suppressing aggregation. Here, we have tested the effectiveness of arginine as an eluent in dye-affinity column chromatography using Blue-Sepharose, which binds enzymes requiring adenyl-containing cofactors...

journal_title：Protein expression and purification

authors： Arakawa T,Ejima D,Tsumoto K,Ishibashi M,Tokunaga M

更新日期：2007-04-01 00:00:00

abstract：:We describe a single method to purify milligram amounts of authentic wild-type and mutant HIV-1 and HIV-2 Tat proteins overexpressed in Escherichia coli. This method takes advantage of the highly basic, positively charged RNA binding domain present in both HIV-1 and HIV-2 Tat, which also facilitated purification of HI...

journal_title：Protein expression and purification

authors： Orsini MJ,García-Martínez LF,Mavankal G,Gaynor RB,Debouck CM

更新日期：1996-09-01 00:00:00

abstract：:Five variants of human β-defensins (HBDs) were expressed in Escherichia coli using two vector systems (pET28a(+) and pQE30) with inducible expression by IPTG. The last vector has not been previously reported as an expression system for HBDs. The recombinant peptides were different in their lengths and overall charge. ...

journal_title：Protein expression and purification

authors： Corrales-Garcia L,Ortiz E,Castañeda-Delgado J,Rivas-Santiago B,Corzo G

更新日期：2013-05-01 00:00:00