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Expression, purification and characterization of cold shock protein A of Corynebacterium pseudotuberculosis.

Abstract：

:The gram-positive bacterium Corynebacterium pseudotuberculosis is the causative agent of different diseases that cause dramatically reduced yields of wool and milk, and results in weight loss, carcass condemnation and also death mainly in sheep, equids, cattle and goats and therefore globally results in considerable economical loss. Cold shock proteins are conserved in many bacteria and eukaryotic cells and they help to restore normal cell functions after cold shock in which some appear to have specific functions at normal growth temperature as well. Cold shock protein A from C. pseudotuberculosis was expressed in Escherichia coli and purified. The thermal unfolding/refolding process characterized by circular dichroism, differential scanning calorimetry and NMR spectroscopy techniques indicated that the refolding process was almost completely reversible.

journal_name

Protein Expr Purif

journal_title

Protein expression and purification

authors

Lindae A,Eberle RJ,Caruso IP,Coronado MA,de Moraes FR,Azevedo V,Arni RK

doi

10.1016/j.pep.2015.04.006

subject

Has Abstract

pub_date

2015-08-01 00:00:00

pages

15-20

eissn

1046-5928

issn

1096-0279

pii

S1046-5928(15)00086-8

journal_volume

112

pub_type

杂志文章

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