A modular polycistronic expression system for overexpressing protein complexes in Escherichia coli.

abstract：:The recently classified group III trypsins include members like Atlantic cod (Gadus morhua) trypsin Y as well as seven analogues from other cold-adapted fish species. The eight group III trypsins have been characterized from their cDNAs and deduced amino acid sequences but none of the enzymes have been isolated from t...

journal_title：Protein expression and purification

authors： Pálsdóttir HM,Gudmundsdóttir A

更新日期：2007-02-01 00:00:00

abstract：:Folding and assembly studies with alpha-helical membrane proteins are often hampered by the absence of high-level expression systems as well as by missing suitable in vitro refolding procedures. Experimental constraints and requirements for heterologous expression and in vitro assembly of cytochrome b6 have been exami...

journal_title：Protein expression and purification

authors： Prodöhl A,Dreher C,Hielscher R,Hellwig P,Schneider D

更新日期：2007-12-01 00:00:00

abstract：:The ELR-CXC chemokines are important to neutrophil inflammation in many acute and chronic diseases. Among them, CXCL8 (interleukin-8, IL-8), binds to both the CXCR1 and CXCR2 receptors with high affinity and the expression levels of CXCL8 are elevated in many inflammatory diseases. Recently, an analogue of human CXCL8...

journal_title：Protein expression and purification

authors： Cheng HT,Huang KC,Yu HY,Gao KJ,Zhao X,Li F,Town J,Gordon JR,Cheng JW

更新日期：2008-09-01 00:00:00

abstract：:Whey acidic proteins (WAP) belong to a large gene family of antibacterial peptides that perform critical immune system functions. The function of human epididymis protein 4 (HE4), a 124-amino acid long polypeptide that has two whey acidic protein four-disulfide core (WFDC) domains, is not well studied. Here, a fusion ...

journal_title：Protein expression and purification

authors： Hua L,Liu Y,Zhen S,Wan D,Cao J,Gao X

更新日期：2014-10-01 00:00:00

abstract：:The main principles of higher-order protein oligomerization are elucidated by many structural and biophysical studies. An astonishing number of proteins self-associate to form dimers or higher-order quaternary structures which further interact with other biomolecules to elicit complex cellular responses. In this study...

journal_title：Protein expression and purification

authors： Pandhare A,Stuebler AG,Pirayesh E,Jansen M

更新日期：2019-01-01 00:00:00

abstract：:α-N-Acetylgalactosaminidase (α-GalNAc-ase; EC.3.2.1.49) is an exoglycosidase specific for the hydrolysis of terminal α-linked N-acetylgalactosamine in various sugar chains. The cDNA corresponding to the α-GalNAc-ase gene was cloned from Aspergillus niger, sequenced, and expressed in the yeast Saccharomyces cerevisiae....

journal_title：Protein expression and purification

authors： Mrázek H,Benada O,Man P,Vaněk O,Křen V,Bezouška K,Weignerová L

更新日期：2012-01-01 00:00:00

abstract：:Cysteine-rich intestinal protein (CRIP) is a small, 8.5-kDa protein with one double zinc-finger motif called a LIM domain. It is very abundant in intestine and some immune cells in rodents, and expression is influenced by development and the immune response. We have cloned a human CRIP cDNA from human small intestine ...

journal_title：Protein expression and purification

authors： Khoo C,Blanchard RK,Sullivan VK,Cousins RJ

更新日期：1997-04-01 00:00:00

abstract：:NF-kappaB is a pleiotropic transcriptional activator originally identified by its ability to regulate immunoglogulin kappa light chain expression. Purification of this DNA-binding complex demonstrated that NF-kappaB is a heterodimer composed of two subunits, NFKB1 and RelA. Previous studies have shown that truncated v...

journal_title：Protein expression and purification

authors： Coleman TA,Huddleston KA,Ruben SM,Rosen CA,Gentz R

更新日期：1997-02-01 00:00:00

abstract：:Antifreeze proteins (AFPs) enable organisms to survive under freezing or sub-freezing conditions. AFPs have a great potential in the low temperature storage of cells, tissues, organs, and foods. This process will require a large number of recombinant AFPs. In the present study, the recombinant carrot AFP was highly ex...

journal_title：Protein expression and purification

authors： Zhang DQ,Liu B,Feng DR,He YM,Wang JF

更新日期：2004-06-01 00:00:00

abstract：:Granulocyte-macrophage colony-stimulating factor (GM-CSF) regulates proliferation, differentiation, and function of hematopoietic progenitor cells. Aside from expansion of hematopoietic cells, GM-CSF has shown efficacy in other diseases, including Crohn's disease. While GM-CSF being clinically used in humans, the abil...

journal_title：Protein expression and purification

authors： Sainathan SK,Tu L,Bishnupuri KS,Han M,Li A,Newberry RD,McDonald KG,Crimmins DL,Houchen C,Anant S,Dieckgraefe BK

更新日期：2005-12-01 00:00:00

abstract：:A method was developed for the affinity purification of human complement properdin. The preparation is part of an integrated scheme in which over 20 human plasma proteins can be recovered in a highly purified form. The yield of properdin was 5.9 mg from 3 liters of plasma, amounting to a 28% recovery. The crucial step...

journal_title：Protein expression and purification

authors： DiScipio RG

更新日期：1994-04-01 00:00:00

abstract：:Human paraoxonase (hPON3) is a high density lipoprotein-related glycoprotein with multi-enzymatic properties and antioxidant activity which is proposed to participate in the prevention of low density lipoprotein (LDL) oxidation. In this study, hPON3 gene was amplified from Human Fetal Liver Marathon-Ready cDNA and exp...

journal_title：Protein expression and purification

authors： Lu H,Zhu J,Zang Y,Ze Y,Qin J

更新日期：2006-03-01 00:00:00

abstract：:Pullulanases are well-known starch-debranching enzymes that are widely used for hydrolysis of a-1,6-glycosidic linkages in starch, pullulan, amylopectin, and other oligosaccharides. Escherichia coli is a popular heterologous expression host for generating target enzymes. However, cells have to be disrupted to obtain t...

journal_title：Protein expression and purification

authors： Wang X,Chen Y,Nie Y,Xu Y

更新日期：2019-03-01 00:00:00

abstract：:Splicing factor proline- and glutamine-rich (SFPQ) is an RNA-binding protein, playing significant roles in gene regulation and subnuclear body formation. Our recent serendipitous discovery showed that SFPQ binds zinc directly and forms an infinite polymer that is induced by zinc binding to the protein. The zinc-induce...

journal_title：Protein expression and purification

authors： Lim YW,Lee M

更新日期：2020-07-01 00:00:00

abstract：:Preventing protein aggregation is crucial for various protein studies, and has a large potential for remedy of protein misfolding or aggregates-linked diseases. In this study, we demonstrated the hyper-acidic protein fusion partners, which were previously reported to enhance the soluble expression of aggregation-prone...

journal_title：Protein expression and purification

authors： Zou Z,Fan Y,Zhang C

更新日期：2011-11-01 00:00:00

abstract：:Keratinocyte growth factor (KGF) is a member of the fibroblast growth factor family that acts specifically on epithelial cells in a paracrine mode. We employed a mammalian expression system to synthesize recombinant human KGF and isolated two preparations, KGF-a and KGF-b, from medium conditioned by Chinese hamster ov...

journal_title：Protein expression and purification

authors： Hsu YR,Hsu EW,Katta V,Brankow D,Tseng J,Hu S,Morris CF,Kenney WC,Lu HS

更新日期：1998-03-01 00:00:00

abstract：:In plant, the first and the third steps of the synthesis of methionine and threonine are catalyzed by a bifunctional enzyme, aspartate kinase-homoserine dehydrogenase (AK-HSDH). In this study, we report the first purification and characterization of a highly active threonine-sensitive AK-HSDH from plants (Arabidopsis ...

journal_title：Protein expression and purification

authors： Paris S,Wessel PM,Dumas R

更新日期：2002-02-01 00:00:00

abstract：:Chinese hamster dihydrofolate reductase (ch-DHFR) was overexpressed in Escherichia coli DH5 alpha under the transcriptional control of PRPL promoters regulated by temperature-sensitive repressors. The desired recombinant product is soluble and constitutes about 30% of the total soluble proteins of the bacterial cell. ...

journal_title：Protein expression and purification

authors： Fan YX,Wang B,Jing GZ

更新日期：1997-03-01 00:00:00

abstract：:Glycolate oxidase is a flavin-dependent enzyme in the photorespiratory pathway in plants. Here we report the heterologous expression of glycolate oxidase in Escherichia coli and an isolation procedure which results in 4 mg pure protein per gram cell paste in only 1.5 days. This corresponds to a more than 50-fold impro...

journal_title：Protein expression and purification

authors： Stenberg K,Lindqvist Y

更新日期：1996-11-01 00:00:00

abstract：:Dengue virus causes serious diseases affecting people in tropical and sub-tropical regions. The nonstructural (NS) protein 2B is an integral membrane protein and important for the regulation of viral protease NS3, which is significant for virus replication. The NS2B-NS3 complex is an important drug target for treating...

journal_title：Protein expression and purification

authors： Huang Q,Chen AS,Li Q,Kang C

更新日期：2011-12-01 00:00:00

abstract：:An internal fragment (978 bp) corresponding to the dog zona pellucida glycoprotein-3 (DZP3), excluding the N-terminal signal sequence and the C-terminal transmembrane-like domain, was amplified by polymerase chain reaction from a full-length cDNA clone. The amplified SacI and PstI restricted fragment was cloned in-fra...

journal_title：Protein expression and purification

authors： Santhanam R,Panda AK,Kumar VS,Gupta SK

更新日期：1998-04-01 00:00:00

abstract：:The Integrator Complex (INT) is a large multi-subunit protein complex, containing at least 14 subunits and a host of associated factors. These protein components have been established through pulldowns of overexpressed epitope tagged subunits or by using antibodies raised against specific subunits. Here, we utilize CR...

journal_title：Protein expression and purification

authors： Baillat D,Russell WK,Wagner EJ

更新日期：2016-12-01 00:00:00

abstract：:During the screening for tyrosine phenol-lyase-producing thermophiles, we isolated an obligatory symbiotic thermophile, Symbiobacterium sp. SC-1, which grew only in coculture with Bacillus sp. SK-1. A gene encoding thermostable tyrosine phenol-lyase (TPL) was cloned from the genomic DNA of the Symbiobacterium sp. SC-1...

journal_title：Protein expression and purification

authors： Lee SG,Hong SP,Choi YH,Chung YJ,Sung MH

更新日期：1997-12-01 00:00:00

abstract：:Chan Su is a traditional medicine prepared from toxic secretions from the auricular and skin glands of Chinese toads. Previous studies show that active components in Chan Su can inhibit the proliferation of tumor cells. To study the effect of Chan Su peptides on angiogenesis, fresh Chan Su was collected and its compon...

journal_title：Protein expression and purification

authors： Xia F,Gao F,Yao H,Zhang G,Gao B,Lu Y,Wang X,Qian Y

更新日期：2019-11-01 00:00:00

abstract：:Notch signaling plays a key role in cell differentiation and is very well conserved from Drosophila to humans. Ligands of Notch receptors are type I, membrane spanning proteins composed of a large extracellular region and a 100-150 residue cytoplasmic tail. We report here, for the first time, the expression, purificat...

journal_title：Protein expression and purification

authors： Popovic M,Coglievina M,Guarnaccia C,Verdone G,Esposito G,Pintar A,Pongor S

更新日期：2006-06-01 00:00:00

abstract：:An expression library was generated from a partial NcoI and HindIII digest of genomic DNA from the thermophilic bacterium, Bacillus stearothermophilus P1. The DNA fragments were cloned into the expression vector pQE-60 and transformed into Escherichia coli M15[EP4]. Sequence analysis of a lipase gene showed an open re...

journal_title：Protein expression and purification

authors： Sinchaikul S,Sookkheo B,Phutrakul S,Pan FM,Chen ST

更新日期：2001-08-01 00:00:00

abstract：:Procedures are described for the preparation of highly purified thymidylate synthases from Escherichia coli and Bacillus subtilis. The yields in each case are quite high with about 350 mg of pure protein obtained from 1 liter of cells. Basically all that is required to obtain pure enzyme is an induction step from a hi...

journal_title：Protein expression and purification

authors： Changchien LM,Garibian A,Frasca V,Lobo A,Maley GF,Maley F

更新日期：2000-07-01 00:00:00

abstract：:Influenza A virus displays one of the highest infection rates of all human viruses and therefore represents a severe human health threat associated with an important economical challenge. Influenza matrix protein 2 (M2) is a membrane protein of the viral envelope that forms a proton selective ion channel. Here we repo...

journal_title：Protein expression and purification

authors： Desuzinges Mandon E,Traversier A,Champagne A,Benier L,Audebert S,Balme S,Dejean E,Rosa Calatrava M,Jawhari A

更新日期：2017-03-01 00:00:00

abstract：:The role of FIC (Filamentation induced by cAMP)(2) domain containing proteins in the regulation of many vital pathways, mostly through the transfer of NMPs from NTPs to specific target proteins (NMPylation), in microorganisms, higher eukaryotes, and plants is emerging. The identity and function of FIC domain containin...

journal_title：Protein expression and purification

authors： Mishra S,Bhagavat R,Chandra N,Vijayarangan N,Rajeswari H,Ajitkumar P

更新日期：2012-11-01 00:00:00

abstract：:Human Fas ligand is a medically important membrane glycoprotein that induces the apoptosis of harmful cells. A new secretory expression and purification method was devised for the production of a large amount of recombinant human Fas ligand extracellular domain (hFasLECD) by Pichia pastoris. The expression plasmid con...

journal_title：Protein expression and purification

authors： Muraki M

更新日期：2006-12-01 00:00:00