Abstract:
:Kynurenine 3-monooxygenase (KMO) is an enzyme central to the kynurenine pathway of tryptophan metabolism. KMO has been implicated as a therapeutic target in several disease states, including Huntington's disease. Recombinant human KMO protein production is challenging due to the presence of transmembrane domains, which localise KMO to the outer mitochondrial membrane and render KMO insoluble in many in vitro expression systems. Efficient bacterial expression of human KMO would accelerate drug development of KMO inhibitors but until now this has not been achieved. Here we report the first successful bacterial (Escherichia coli) expression of active FLAG™-tagged human KMO enzyme expressed in the soluble fraction and progress towards its purification.
journal_name
Protein Expr Purifjournal_title
Protein expression and purificationauthors
Wilson K,Mole DJ,Binnie M,Homer NZ,Zheng X,Yard BA,Iredale JP,Auer M,Webster SPdoi
10.1016/j.pep.2013.11.015subject
Has Abstractpub_date
2014-03-01 00:00:00pages
96-103eissn
1046-5928issn
1096-0279pii
S1046-5928(13)00259-3journal_volume
95pub_type
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