Abstract:
:A cysteine-rich (approximately 20%), low molecular weight (MW 6 kDa) polypeptide has been isolated from the Korean blood-sucking leech, Hirudo nipponia. From its amino acid composition and N-terminal amino acid sequence analysis, the new protein is similar to granulin (or epithelin), and so it has been named leech granulin. The leech granulin behaved as a thrombin inhibitor in the purification steps of size-exclusion, ion-exchange, chromatofocusing, and reverse-phase high-performance liquid chromatography. The leech granulin is an acidic peptide (pI 3.75) containing high cysteine residues with a unique sequence similar to granulins or epithelins isolated from other organisms. For the first time, a granulin-like polypeptide having thrombin inhibitory activity has been isolated from a leech species.
journal_name
Protein Expr Purifjournal_title
Protein expression and purificationauthors
Hong SJ,Kang KWdoi
10.1006/prep.1999.1077keywords:
subject
Has Abstractpub_date
1999-07-01 00:00:00pages
340-6issue
2eissn
1046-5928issn
1096-0279pii
S104659289991077Xjournal_volume
16pub_type
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