Abstract：
:Arginine has been effectively used in various column chromatographies for improving recovery and resolution, and suppressing aggregation. Here, we have tested the effectiveness of arginine as an eluent in dye-affinity column chromatography using Blue-Sepharose, which binds enzymes requiring adenyl-containing cofactors (e.g., NAD). A common eluent, NaCl, showed a broad elution peak with low recovery of lactate dehydrogenase, at most approximately 60% using 2M salt. The recovery decreased as the NaCl concentration was either decreased or increased; i.e., the recovery was maximum at 2M. On the contrary, addition of arginine to the eluent resulted in more than 80% recovery above 0.5M and the recovery was nearly independent of the arginine concentration. The elution peak was much sharper with arginine, leading to elution of more concentrated protein solution. Successful elution of proteins bound to the ATP-agarose resins by arginine was also described.
journal_name
Protein Expr Purifjournal_title
Protein expression and purificationauthors
Arakawa T,Ejima D,Tsumoto K,Ishibashi M,Tokunaga Mdoi
10.1016/j.pep.2006.10.005subject
Has Abstractpub_date
2007-04-01 00:00:00pages
410-4issue
2eissn
1046-5928issn
1096-0279pii
S1046-5928(06)00318-4journal_volume
52pub_type
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journal_title：Protein expression and purification
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journal_title：Protein expression and purification
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journal_title：Protein expression and purification
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journal_title：Protein expression and purification
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journal_title：Protein expression and purification
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