Expression of Sonic hedgehog-Fc fusion protein in Pichia pastoris. Identification and control of post-translational, chemical, and proteolytic modifications.

abstract：:Pyrroline-5-carboxylate reductase (P5CR) catalyzes the reduction of Delta1-pyrroline-5-carboxylate (P5C) to proline with concomitant oxidation of NAD(P)H to NAD(P)(+). The enzymatic cycle between P5C and proline is very important in many physiological and pathological processes. Human P5CR was over-expressed in Escher...

journal_title：Protein expression and purification

authors： Meng Z,Lou Z,Liu Z,Hui D,Bartlam M,Rao Z

更新日期：2006-09-01 00:00:00

abstract：:A stable mammalian cell line expressing highly active bovine interferon-gamma (BoIFN-γ) was generated using Flp recombinase-mediated integration. This recombinant 293 cell line (B1) efficiently secreted FLAG-tagged BoIFN-γ protein into the culture supernatant, as determined by ELISA and Western blot. The recombinant B...

journal_title：Protein expression and purification

authors： Xu Z,Shan F,Shan F,Meng C,Zhou X,Zhang X,Chen X,Jiao X

更新日期：2014-07-01 00:00:00

abstract：:The Integrator Complex (INT) is a large multi-subunit protein complex, containing at least 14 subunits and a host of associated factors. These protein components have been established through pulldowns of overexpressed epitope tagged subunits or by using antibodies raised against specific subunits. Here, we utilize CR...

journal_title：Protein expression and purification

authors： Baillat D,Russell WK,Wagner EJ

更新日期：2016-12-01 00:00:00

abstract：:A maize dehydrin with an apparent molecular weight of 20 kDa was purified from whole kernels of maize inbred line G50. Kernels were ground in a seed mill, stirred overnight in extraction buffer, and centrifuged to extract soluble proteins. The sample was heated to 89 degrees C and centrifuged to remove heat-insoluble ...

journal_title：Protein expression and purification

authors： Ceccardi TL,Meyer NC,Close TJ

更新日期：1994-06-01 00:00:00

abstract：:Dengue virus causes serious diseases affecting people in tropical and sub-tropical regions. The nonstructural (NS) protein 2B is an integral membrane protein and important for the regulation of viral protease NS3, which is significant for virus replication. The NS2B-NS3 complex is an important drug target for treating...

journal_title：Protein expression and purification

authors： Huang Q,Chen AS,Li Q,Kang C

更新日期：2011-12-01 00:00:00

abstract：:HtrA2 is an apoptosis-activating protein to enhance the apoptotic process by preventing the formation of the IAP-caspase complex, thus freeing caspase to trigger the apoptosis pathway. Here, we presented the full-length sequence of HtrA2 from the black tiger shrimp (PmHtrA2). The full-length PmHtrA2 transcript was 140...

journal_title：Protein expression and purification

authors： Suwannaboon R,Phiwsaiya K,Senapin S,Khunrae P,Rattanarojpong T

更新日期：2013-12-01 00:00:00

abstract：:Fab (fragment that having the antigen binding site) of a monoclonal antibody (mAb) is widely required in biopharmaceutical research and development. At Centocor, two routes of Fab production and purification were used to enable a variety of research and development efforts, particularly, crystallographic studies of an...

journal_title：Protein expression and purification

authors： Zhao Y,Gutshall L,Jiang H,Baker A,Beil E,Obmolova G,Carton J,Taudte S,Amegadzie B

更新日期：2009-10-01 00:00:00

abstract：:Receptor activator of NF-kappaB ligand (RANKL) is a type II transmembrane protein found on osteoblasts which functions as a major determinant of osteoclast differentiation and activation. RANKL mediates bone homeostasis through binding to the cognate ligand on osteoclasts, RANK, and a soluble decoy receptor, osteoprot...

journal_title：Protein expression and purification

authors： Willard D,Chen WJ,Barrett G,Blackburn K,Bynum J,Consler T,Hoffman C,Horne E,Iannone MA,Kadwell S,Parham J,Ellis B

更新日期：2000-10-01 00:00:00

abstract：:The cDNA for the alpha i1 protein that had undergone site-directed mutagenesis to change glycine-2 to alanine was ligated into a baculovirus transfer vector. A recombinant virus was obtained by transfecting Sf9 cells with both the wild-type baculovirus DNA and the transfer vector and screening for recombinant plaques....

journal_title：Protein expression and purification

authors： Jones TL,Woodard C,Spiegel AM

更新日期：1993-02-01 00:00:00

abstract：:Triacylglycerol hydrolase mobilizes stored triacylglycerol some of which is used for very-low-density lipoprotein assembly in the liver. A full-length cDNA coding for a human triacylglycerol hydrolase (hTGH) was isolated from a human liver cDNA library. The cDNA has an open reading frame of 576 amino acids with a clea...

journal_title：Protein expression and purification

authors： Alam M,Ho S,Vance DE,Lehner R

更新日期：2002-02-01 00:00:00

abstract：:The role of the termini of protein sequences is often perturbed by remnant amino acids after the specific protease cleavage of the affinity tags and/or by the amino acids encoded by the plasmid at/around the restriction enzyme sites used to insert the genes. Here we describe a method for affinity purification of a met...

journal_title：Protein expression and purification

authors： Abd Elhameed HAH,Hajdu B,Balogh RK,Hermann E,Hunyadi-Gulyás É,Gyurcsik B

更新日期：2019-07-01 00:00:00

abstract：:Human prolactin was expressed in insect culture cells by recombinant baculoviruses carrying prolactin gene cDNA placed under the transcriptional control of polyhedrin gene promoter of Autographa californica nuclear polyhedrosis virus. Preliminary results of recombinant human prolactin expression as extracellular as we...

journal_title：Protein expression and purification

authors： Strokovskaya L,Bartoszewicz Z,Szolajska E,Kikhno I,Solomko A,Michalik J

更新日期：2001-07-01 00:00:00

abstract：:Folding and assembly studies with alpha-helical membrane proteins are often hampered by the absence of high-level expression systems as well as by missing suitable in vitro refolding procedures. Experimental constraints and requirements for heterologous expression and in vitro assembly of cytochrome b6 have been exami...

journal_title：Protein expression and purification

authors： Prodöhl A,Dreher C,Hielscher R,Hellwig P,Schneider D

更新日期：2007-12-01 00:00:00

abstract：:Bikunin is a proteoglycan exhibiting broad-spectrum inhibitory activity against serine proteases and could potentially suppress tumor cell invasion and metastasis. Here, we have successfully expressed recombinant human bikunin (rh-bikunin) in the methylotrophic yeast Pichia pastoris and established the purification pr...

journal_title：Protein expression and purification

authors： Jian-qiu W,Feng-qin Y,Dou-dou W,Lei B,Nan S,Cong-yan L,Ting Z,Wei-qun Y

更新日期：2008-08-01 00:00:00

abstract：:The expression of human lactoferrin in the mammary gland is an attractive approach to diminish its current production cost. Previous attempts to produce lactorferrin in the milk of transgenic animals resulted in very high cost and uncertain results. In this paper, we have directly infused replication-defective adenovi...

journal_title：Protein expression and purification

authors： Han ZS,Li QW,Zhang ZY,Xiao B,Gao DW,Wu SY,Li J,Zhao HW,Jiang ZL,Hu JH

更新日期：2007-05-01 00:00:00

abstract：:Developmentally regulated G-proteins (DRGs) are a highly conserved family of GTP-binding proteins found in archaea, plants, fungi and animals, indicating important roles in fundamental pathways. Their function is poorly understood, but they have been implicated in cell division, proliferation, and growth, as well as s...

journal_title：Protein expression and purification

authors： O'Connell A,Robin G,Kobe B,Botella JR

更新日期：2009-10-01 00:00:00

abstract：:Sphingomyelinase C (SMC) of the actinomycete, Streptomycesgriseocarneus NBRC13471, was constitutively expressed to high levels using Streptomyces lividans host and thereafter was extracellularly secreted into the cell culture. Purified SMC had a high specific activity (approximately 550-950 U/mg) and was obtained in h...

journal_title：Protein expression and purification

authors： Sugimori D,Matsumoto Y,Tomita Y,Murayama K,Ogino C

更新日期：2012-02-01 00:00:00

abstract：:We have previously reported that a variety of solid human tumor cell lines express a large number of receptors for interleukin-13 (IL-13). These receptors could be targeted with a chimeric fusion protein consisting of human IL-13 and a truncated form of Pseudomonas exotoxin (PE). We describe here optimization of criti...

journal_title：Protein expression and purification

authors： Joshi BH,Puri RK

更新日期：2005-02-01 00:00:00

abstract：:The plant protein production system is a platform that can not only reduce production costs but also produce monoclonal antibodies that do not have the risk of residual proteins from the host. However, due to the difference between post-translational processes in plants and animals, there may be a modification in the ...

journal_title：Protein expression and purification

authors： Jin N,Lee JW,Heo W,Ryu MY,So MK,Ko BJ,Kim HY,Yoon SM,Lee J,Kim JY,Kim WT

更新日期：2019-07-01 00:00:00

abstract：:Alphavirus nonstructural protein nsP1 possesses distinct methyltransferase (MTase) and guanylyltransferase (GTase) activities involved in the capping of viral RNAs. In alphaviruses, the methylation of GTP occurs before RNA transguanylation and nsP1 forms a covalent complex with m(7)GMP unlike the host mRNA guanylyltra...

journal_title：Protein expression and purification

authors： Tomar S,Narwal M,Harms E,Smith JL,Kuhn RJ

更新日期：2011-10-01 00:00:00

abstract：:An active form of a single-chain antibody fragment (scFv) from the murine monoclonal antibody ABL-1, which is specific for B-cell-activating factor of the TNF family, was produced in Escherichia coli. The complementary DNAs encoding the variable regions of the heavy chain (VH) and light chain (VL) were connected by a ...

journal_title：Protein expression and purification

authors： Cao P,Tang XM,Guan ZB,Diao ZY,Zhang SQ

更新日期：2005-10-01 00:00:00

abstract：:Periplasmic expression of recombinant proteins ensures the production of biologically active proteins in a correctly folded state with several key advantages. This research focused on the in-frame cloning of rhIL-15 in pET-20 (+) vector with pelB-leader sequence to direct the protein to the bacterial periplasm. The ta...

journal_title：Protein expression and purification

authors： Ahmed N,Afroze B,Abbas R,Khan MA,Akram M,Tahir S,Bakht S,Munir A,Shahid AA

更新日期：2021-01-01 00:00:00

abstract：:Acyl carrier protein (ACP) was purified from Euglena gracilis variety bacillaris in yields of about 1 mg/100 g (wet wt) of cells. Antibodies against the purified protein were raised in hens and isolated from eggs. Antibodies raised against Euglena ACP inhibited the Euglena chloroplast nonaggregated fatty acid syntheta...

journal_title：Protein expression and purification

authors： Williams SG,Worsham LM,Ernst-Fonberg ML

更新日期：1991-04-01 00:00:00

abstract：:The aim of this study was to introduce a simple, reproducible, and less expensive method for isolation of alpha-lactalbumin, beta-lactoglobulin, and bovine serum albumin from cow's milk while retaining their antigenicity. Whey (lactoserum) was obtained by isolating casein from defatted milk using hydrochloric acid. Gl...

journal_title：Protein expression and purification

authors： Neyestani TR,Djalali M,Pezeshki M

更新日期：2003-06-01 00:00:00

abstract：:The bivalent anti-human anti-T cell immunotoxin A-dmDT390-bisFv(G(4)S) was developed for treatment of T cell leukemia, autoimmune diseases, and tolerance induction for transplantation. The multi-domain structure of the bivalent immunotoxin hinders efficient production in Escherichia coli and most eukaryotes are sensit...

journal_title：Protein expression and purification

authors： Woo JH,Liu YY,Mathias A,Stavrou S,Wang Z,Thompson J,Neville DM Jr

更新日期：2002-07-01 00:00:00

abstract：:The basidiomycete Phanerochaete chrysosporium produces several isoforms of lignin peroxidase, which catalyzes the oxidative depolymerization of lignin To date, ion-exchange chromatography and preparative isoelectric focusing (IEF) have been commonly used for isolation of lignin peroxidase isoenzymes. In this work we h...

journal_title：Protein expression and purification

authors： Ollikka P,Leppänen VM,Anttila T,Suominen I

更新日期：1995-06-01 00:00:00

abstract：:Large amounts of soluble fibronectin were easily purified from cryoprecipitated or fresh citrated human blood plasma by a three-step combination of gelatin and heparin-cellufine affinity chromatography. The elution conditions were optimized to obtain a homogeneous fraction on SDS-PAGE and Western blot under reducing c...

journal_title：Protein expression and purification

authors： Poulouin L,Gallet O,Rouahi M,Imhoff JM

更新日期：1999-10-01 00:00:00

abstract：:The Plasmodium falciparum cysteine protease falcipain-2 is a potential new target for antimalarial chemotherapy. In order to obtain large quantities of active falcipain-2 for biochemical and structural analysis, a systematic assessment of optimal parameters for the expression and refolding of the protease was carried ...

journal_title：Protein expression and purification

authors： Sijwali PS,Brinen LS,Rosenthal PJ

更新日期：2001-06-01 00:00:00

abstract：:We describe an efficient strategy to produce high-quality proteins by using a single large IMAC chromatography column and enzymatic His-tag removal via the TAGZyme system in pilot scale. Numerous quality assays demonstrated a high purity of the final product, the human cytokine Interleukin-1beta (IL-1beta). The protei...

journal_title：Protein expression and purification

authors： Block H,Kubicek J,Labahn J,Roth U,Schäfer F

更新日期：2008-02-01 00:00:00

abstract：:Silent information regulator 2 (Sir2) proteins are a class of protein deacetylase enzymes that play key roles in transcriptional gene silencing, DNA repair, and aging. Here, we describe the high-level bacterial expression and purification of a human SirT2 construct that yields high resolution NMR spectra. By removing ...

journal_title：Protein expression and purification

authors： Sun C,Song D,Marcotte PA,Richardson PL,Hajduk PJ

更新日期：2006-07-01 00:00:00