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Expression, purification, and characterization of the human receptor activator of NF-kappaB ligand (RANKL) extracellular domain.

Abstract：

:Receptor activator of NF-kappaB ligand (RANKL) is a type II transmembrane protein found on osteoblasts which functions as a major determinant of osteoclast differentiation and activation. RANKL mediates bone homeostasis through binding to the cognate ligand on osteoclasts, RANK, and a soluble decoy receptor, osteoprotegerin (OPG). We designed a construct encoding the extracellular domain of human RANKL that conformed to reports of native processing. To encourage folding and posttranslational modification of a normally membrane-inserted moiety, we expressed the RANKL truncate as a secreted protein using the signal sequence from OPG in a Trichoplusia ni cell line using a baculovirus expression vector. RANKL was purified by a three-step process including an OPG-Fc affinity column. SDS-PAGE and mass spectral analysis indicated that the protein was >99% pure and glycosylated. Circular dichroism spectra revealed that the protein exhibited structural elements similar to tumor necrosis factor-alpha. By BIAcore analysis, RANKL bound to OPG with an affinity of 6.7 nM. Sedimentation equilibrium analytical ultracentrifugation analyses established that our protein existed as a trimer. We conclude that our expressed human RANKL truncate is folded, is functional, and exhibits self-association consistent with other family members.

journal_name

Protein Expr Purif

journal_title

Protein expression and purification

authors

Willard D,Chen WJ,Barrett G,Blackburn K,Bynum J,Consler T,Hoffman C,Horne E,Iannone MA,Kadwell S,Parham J,Ellis B

doi

10.1006/prep.2000.1278

keywords:

subject

Has Abstract

pub_date

2000-10-01 00:00:00

pages

48-57

issue

1

eissn

1046-5928

issn

1096-0279

pii

S1046-5928(00)91278-6

journal_volume

20

pub_type

杂志文章

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