Purification, characterization, and crystallization of human pyrroline-5-carboxylate reductase.

abstract：:Lectins are a class of proteins with specific carbohydrate-binding properties found in a wide variety of plants and animals. Gramineae lectins are presumably defense-related proteins in plants that exert their effect by binding to N-acetylglucosamine. Barley lectin is a vacuolar protein synthesized with an amino-termi...

journal_title：Protein expression and purification

authors： Schroeder MR,Raikhel NV

更新日期：1992-12-01 00:00:00

abstract：:High-level constitutive expression of xylanase GH11 from Penicillium occitanis Pol6 termed PoXyn2 was achieved using the methylotrophic yeast Pichia pastoris. The PoXyn2 cDNA encoding for a mature xylanase of 320 amino acids was subcloned into the pGAPZαA vector, to construct recombinant xylanse with six histidine res...

journal_title：Protein expression and purification

authors： Driss D,Bhiri F,Ghorbel R,Chaabouni SE

更新日期：2012-05-01 00:00:00

abstract：:The mRNA encoding the 51-kDa subunit of 6-phosphogluconate dehydrogenase (6PGDH) from sheep liver was reverse-transcribed and amplified. The resulting cDNA was reamplified in N-terminal and C-terminal segments and spliced to generate a full-length clone, and an internal cDNA fragment was also amplified. The full-lengt...

journal_title：Protein expression and purification

authors： Chooback L,Price NE,Karsten WE,Nelson J,Sundstrom P,Cook PF

更新日期：1998-07-01 00:00:00

abstract：:Cell extracts of Clostridium kluyveri grown on ethanol plus succinate contained a NAD(H) dependent 4-hydroxybutanoate dehydrogenase (EC 1.1.1.61) at 66 U/mg. This enzyme was purified 42-fold under anaerobic conditions to homogeneity. Heat treatment, ion exchange chromatography on DEAE-cellulose, nondenaturing polyacry...

journal_title：Protein expression and purification

authors： Wolff RA,Kenealy WR

更新日期：1995-04-01 00:00:00

abstract：:Borneol is a bicyclic plant monoterpene. It can be degraded by soil microorganisms through the conversion of borneol dehydrogenase (BDH) and a known camphor degradation pathway. Recombinant BDH from Pseudomonas sp. TCU-HL1 was produced in the form of inclusion body. The refolded BDH1 tends to precipitate. Insoluble re...

journal_title：Protein expression and purification

authors： Khine AA,Lu PC,Ko TP,Huang KF,Chen HP

更新日期：2020-11-01 00:00:00

abstract：:The high level expression and purification of rat monoamine oxidase B (rMAOB) in the methylotrophic yeast Pichia pastoris is reported. Nearly 100 mg of purified rMAOB is obtained from 130 g (wet weight) of cells (0.5 L of culture). The MALDI-TOF mass spectrum of the purified protein shows a single species with a molec...

journal_title：Protein expression and purification

authors： Upadhyay AK,Edmondson DE

更新日期：2008-06-01 00:00:00

abstract：:The present report documents an improved enzyme assay method for the mammalian L-alanine:4,5-dioxovalerate transaminase which is of significant utility in work with crude tissue homogenates, cell cultures, or purified enzyme preparations. We also describe a new and rapid purification procedure for this enzyme from rat...

journal_title：Protein expression and purification

authors： Tyagi RK,Datta K

更新日期：1994-12-01 00:00:00

abstract：:Staphylococcal enterotoxins (SEs) are powerful superantigens that stimulate non-specific T-cell proliferation produced by Staphylococcus aureus and draw considerable attention as ideal drugs for cancer therapy. The filtrate of S. aureus culture has been used as ampul named Staphylococcal enterotoxin C injection in cli...

journal_title：Protein expression and purification

authors： Pan YQ,Ding D,Li DX,Chen SQ

更新日期：2007-12-01 00:00:00

abstract：:Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit epsilon N-methyltransferase (Protein methylase III, Rubisco LSMT, EC 2.1.1.43) catalyzes methylation of the epsilon-amino group of Lys-14 in the large subunit of Rubisco. In this paper, an affinity purification procedure for pea (Pisum sativum L. cv Laxton'...

journal_title：Protein expression and purification

authors： Wang P,Royer M,Houtz RL

更新日期：1995-08-01 00:00:00

abstract：:Escherichia coli oligoribonuclease (EcoORN), encoded by the orn gene, is a 3'-5' exonuclease that degrades short single-stranded oligoribonucleotides to rNMPs in the final step of RNA degradation. The orn gene is essential in E. coli, but not in higher organisms, and close homologues are present in other genomes from ...

journal_title：Protein expression and purification

authors： Young Park A,Elvin CM,Hamdan SM,Wood RJ,Liyou NE,Hamwood TE,Jennings PA,Dixon NE

更新日期：2008-02-01 00:00:00

abstract：:The TCL1 gene, which is located on chromosome 14, plays a major role in human hematopoietic malignancies and encodes a 14-kDa protein whose function has not been determined. This gene is expressed in pre-B cells, in immature thymocytes, and, at low levels, in activated T cells but not in peripheral mature B cells and ...

journal_title：Protein expression and purification

authors： Du Bois GC,Song SP,Kulikovskaya I,Rothstein JL,Germann MW,Croce CM

更新日期：2000-04-01 00:00:00

abstract：:We have developed a family of cloning vectors that direct expression of fusion proteins that mimic aggregated immunoglobulin (IgG) (AIG) and immune complex function with respect to their interactions with FcgammaR and that allow for the inclusion and targeting of a second protein domain to cells expressing FcgammaR. T...

journal_title：Protein expression and purification

authors： White DM,Jensen MA,Shi X,Qu Zx,Arnason BG

更新日期：2001-04-01 00:00:00

abstract：:Influenza A virus displays one of the highest infection rates of all human viruses and therefore represents a severe human health threat associated with an important economical challenge. Influenza matrix protein 2 (M2) is a membrane protein of the viral envelope that forms a proton selective ion channel. Here we repo...

journal_title：Protein expression and purification

authors： Desuzinges Mandon E,Traversier A,Champagne A,Benier L,Audebert S,Balme S,Dejean E,Rosa Calatrava M,Jawhari A

更新日期：2017-03-01 00:00:00

abstract：:Plantaricin JK (PlnJK) is a Class IIb LAB bacteriocin that includes two peptides; i.e., PlnJ and PlnK, which can synergistically halt many types of gram-positive bacteria, including food spoilage organisms. Purification of these peptides from natural lactic acid bacteria is difficult therefore, their application remai...

journal_title：Protein expression and purification

authors： Xu Y,Yang L,Li P,Gu Q

更新日期：2019-07-01 00:00:00

abstract：:Arginine has been effectively used in various column chromatographies for improving recovery and resolution, and suppressing aggregation. Here, we have tested the effectiveness of arginine as an eluent in dye-affinity column chromatography using Blue-Sepharose, which binds enzymes requiring adenyl-containing cofactors...

journal_title：Protein expression and purification

authors： Arakawa T,Ejima D,Tsumoto K,Ishibashi M,Tokunaga M

更新日期：2007-04-01 00:00:00

abstract：:Huperzine A (HupA) is a drug used for the treatment of Alzheimer's disease. However, the biosynthesis of this medicinally important compound is not well understood. The HupA biosynthetic pathway is thought to be initiated by the decarboxylation of lysine to form cadaverine, which is then converted to 5-aminopentanal b...

journal_title：Protein expression and purification

authors： Yang H,Peng S,Zhang Z,Yan R,Wang Y,Zhan J,Zhu D

更新日期：2016-12-01 00:00:00

abstract：:The recently classified group III trypsins include members like Atlantic cod (Gadus morhua) trypsin Y as well as seven analogues from other cold-adapted fish species. The eight group III trypsins have been characterized from their cDNAs and deduced amino acid sequences but none of the enzymes have been isolated from t...

journal_title：Protein expression and purification

authors： Pálsdóttir HM,Gudmundsdóttir A

更新日期：2007-02-01 00:00:00

abstract：:Expression and purification of human beta-secretase (BACE1) in bacteria have been plagued with issues concerning solubility, inhomogeneous N-terminus, and lack of enzymic activity. Several forms of the mature human BACE1 have been expressed in Escherichia coli with different N-terminal extensions and without the C-ter...

journal_title：Protein expression and purification

authors： Sardana V,Xu B,Zugay-Murphy J,Chen Z,Sardana M,Darke PL,Munshi S,Kuo LC

更新日期：2004-04-01 00:00:00

abstract：:Optimization of coding sequences to maximize protein expression yield is often outsourced to external service providers during commercial gene synthesis and thus unfortunately remains a black box for many researchers. The presented software program "CodonWizard" offers scientists a powerful but easy-to-use tool for cu...

journal_title：Protein expression and purification

authors： Rehbein P,Berz J,Kreisel P,Schwalbe H

更新日期：2019-08-01 00:00:00

abstract：:Manganese superoxide dismutase (Mn-SOD) is one of the major enzymes responsible for the defense against oxidative damage due to reactive oxygen species (ROS) in the mitochondria. The present study aimed to produce and evaluate the genetically engineered manganese superoxide dismutase protein. A recombinant plasmid con...

journal_title：Protein expression and purification

authors： Feng W,Mei S,Wenjie Y,Luyuan H

更新日期：2011-05-01 00:00:00

abstract：:The cyclin-dependent kinase-activating kinase (CAK) catalyzes the phosphorylation of the cyclin-dependent protein kinases (CDKs) on a threonine residue (Thr160 in human CDK2). The reaction is an obligatory step in the activation of the CDKs. In higher eukaryotes, the CAK complex has been characterized in two forms. Th...

journal_title：Protein expression and purification

authors： Lawrie AM,Tito P,Hernandez H,Brown NR,Robinson CV,Endicott JA,Noble ME,Johnson LN

更新日期：2001-11-01 00:00:00

abstract：:Disulfide bonds are normally formed after a polypeptide has been exported from the reducing environment of the cytoplasm into a more oxidizing compartment, such as the bacterial periplasm. Recently, we showed that in Escherichia coli trxB gor mutants, in which the reduction of thioredoxin and glutathione is impaired, ...

journal_title：Protein expression and purification

authors： Levy R,Weiss R,Chen G,Iverson BL,Georgiou G

更新日期：2001-11-01 00:00:00

abstract：:The Pseudomonas sp. have been long recognized for their exogenous lipolytic activities yet the genus still contains a lot of unexplored strains. Due to the versatile metabolic machinery and their potential for adaptation to fluctuating environmental conditions Pseudomonas sp. are of great interest for biotechnological...

journal_title：Protein expression and purification

authors： Priyanka P,Kinsella G,Henehan GT,Ryan BJ

更新日期：2019-01-01 00:00:00

abstract：:For affinity-chromatography-based purification of proteins that are prone to abnormal termination of translation or that may not be modified at their N-termini, affinity tags are needed which can be fused to the C-terminus. In this publication we describe that maltose binding protein (MBP) fused to the C-terminus of t...

journal_title：Protein expression and purification

authors： Hennig L,Schäfer E

更新日期：1998-12-01 00:00:00

abstract：:The high specific lysyl endopeptidase (Lys-C; EC 3.4.21.50) is often used for the initial fragmentation of polypeptide chains during protein sequence analysis. However, due to its specificity it could be a useful tool for the production of tailor-made protein hydrolysates with for example bioactive or techno functiona...

journal_title：Protein expression and purification

authors： Stressler T,Eisele T,Meyer S,Wangler J,Hug T,Lutz-Wahl S,Fischer L

更新日期：2016-02-01 00:00:00

abstract：:Stem cell factor (SCF) known as the c-kit ligand is a two disulfide bridge-containing cytokine in the regulation of the development and function of hematopoietic cell lineages and other cells such as mast cells, germ cells, and melanocytes. The secreted soluble form of SCF exists as noncovalently associated homodimer ...

journal_title：Protein expression and purification

authors： Akuta T,Kikuchi-Ueda T,Imaizumi K,Oshikane H,Nakaki T,Okada Y,Sultana S,Kobayashi K,Kiyokawa N,Ono Y

更新日期：2015-01-01 00:00:00

abstract：:The human melanocortin 4 receptor (MC4r) was successfully expressed in Sf9 cells using the baculovirus infection system. N- and C-terminally His-tagged receptors generated B(max) values of 14 and 23 pmol receptor/mg membrane protein, respectively. The highest expression level obtained with the C-terminally His-tagged ...

journal_title：Protein expression and purification

authors： Dolby V,Collén A,Lundqvist A,Cronet P

更新日期：2004-10-01 00:00:00

abstract：:Receptor tyrosine kinase like orphan receptor 2 (ROR2) is a co-receptor for some Wnt proteins including Wnt5a that activate the noncanonical Wnt/planar cell polarity (PCP) signaling pathway. Upregulation of ROR2 is associated with several cancer forms. The extracellular region of ROR2, which contains an immunoglobulin...

journal_title：Protein expression and purification

authors： Li Y,Han X,Xu W,Rao Z,Li X

更新日期：2019-06-01 00:00:00

abstract：:Protein convertase 1/3 is a serine endoproteinase present in the regulated secretory pathway of endocrine and neuroendocrine cells. It is responsible for the processing of numerous prohormones and proneuropeptides into their biologically active moieties, often following cleavage at pairs of basic residues. The determi...

journal_title：Protein expression and purification

authors： Rabah N,Gauthier DJ,Gauthier D,Lazure C

更新日期：2004-10-01 00:00:00

abstract：:Single-stranded DNA-binding proteins (SSBs) play essential roles in DNA replication, recombination, and repair in bacteria, archaea, and eukarya. We report here the identification, expression, and purification of the SSB-like proteins of the thermophilic bacteria Thermus thermophilus and T. aquaticus. The nucleotide (...

journal_title：Protein expression and purification

authors： Dabrowski S,Olszewski M,Piatek R,Kur J

更新日期：2002-10-01 00:00:00