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Expression and bioactivity analysis of Staphylococcal enterotoxin M and N.

Abstract：

:Staphylococcal enterotoxins (SEs) are powerful superantigens that stimulate non-specific T-cell proliferation produced by Staphylococcus aureus and draw considerable attention as ideal drugs for cancer therapy. The filtrate of S. aureus culture has been used as ampul named Staphylococcal enterotoxin C injection in clinic for 10 years in China and proved to be effective. The superantigen SEC claimed to be the only active component without certifiable evidences. For further investigations of the active components of this injection and establishment of foundations for the development of novel anti-cancer drugs, in this research we extracted total DNA from S. aureus (FRI 1230), cloned, expressed and purified recombinant proteins of Staphylococcal enterotoxin M and N (rSEM and rSEN). The MTT assay of the purified rSEM and rSEN demonstrated that their abilities of stimulating T cells and inhibiting the proliferation of K562-ADM cells and B16 cells were equivalent to that of purified SEC2 in vitro. These findings suggested that SEC was not the only active component of Staphylococcal enterotoxin C injection and the effective procedure of expression and purification may be useful for mass productions of these therapeutically important proteins.

journal_name

Protein Expr Purif

journal_title

Protein expression and purification

authors

Pan YQ,Ding D,Li DX,Chen SQ

doi

10.1016/j.pep.2007.08.005

subject

Has Abstract

pub_date

2007-12-01 00:00:00

pages

286-92

issue

2

eissn

1046-5928

issn

1096-0279

pii

S1046-5928(07)00197-0

journal_volume

56

pub_type

杂志文章

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