The heterologous expression strategies of antimicrobial peptides in microbial systems.

Abstract:

:Antimicrobial peptides (AMPs) consist of molecules acting on the defense systems of numerous organisms toward tumor and multiple pathogens, such as bacteria, fungi, viruses, and parasites. Compared to traditional antibiotics, AMPs are more stable and have lower propensity for developing resistance through functioning in the innate immune system, thus having important applications in the fields of medicine, food and so on. However, despite of their high economic values, the low yield and the cumbersome extraction process in AMPs production are problems that limit their industrial application and scientific research. To conquer these obstacles, optimized heterologous expression technologies were developed that could provide effective ways to increase the yield of AMPs. In this review, the research progress on heterologous expression of AMPs using Escherichia coli, Bacillus subtilis, Pichia pastoris and Saccharomyces cerevisiae as host cells was mainly summarized, which might guide the expression strategies of AMPs in these cells.

journal_name

Protein Expr Purif

authors

Deng T,Ge H,He H,Liu Y,Zhai C,Feng L,Yi L

doi

10.1016/j.pep.2017.08.003

subject

Has Abstract

pub_date

2017-12-01 00:00:00

pages

52-59

eissn

1046-5928

issn

1096-0279

pii

S1046-5928(17)30419-9

journal_volume

140

pub_type

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