The heterologous expression strategies of antimicrobial peptides in microbial systems.


:Antimicrobial peptides (AMPs) consist of molecules acting on the defense systems of numerous organisms toward tumor and multiple pathogens, such as bacteria, fungi, viruses, and parasites. Compared to traditional antibiotics, AMPs are more stable and have lower propensity for developing resistance through functioning in the innate immune system, thus having important applications in the fields of medicine, food and so on. However, despite of their high economic values, the low yield and the cumbersome extraction process in AMPs production are problems that limit their industrial application and scientific research. To conquer these obstacles, optimized heterologous expression technologies were developed that could provide effective ways to increase the yield of AMPs. In this review, the research progress on heterologous expression of AMPs using Escherichia coli, Bacillus subtilis, Pichia pastoris and Saccharomyces cerevisiae as host cells was mainly summarized, which might guide the expression strategies of AMPs in these cells.


Protein Expr Purif


Deng T,Ge H,He H,Liu Y,Zhai C,Feng L,Yi L




Has Abstract


2017-12-01 00:00:00












  • Purification, characterization, and crystallization of the distal BRCT domain of the human XRCC1 DNA repair protein.

    abstract::The XRCC1 DNA repair protein contains two regions of approximately 100 amino acids each that share homology with the BRCT (BRCA1 carboxyl terminus) domain superfamily. These two regions of XRCC1 have been shown to interact independently with DNA ligase III and poly(ADP-ribose)polymerase as part of a mechanism involved...

    journal_title:Protein expression and purification

    pub_type: 杂志文章


    authors: Thornton K,Forstner M,Shen MR,West MG,Rupp B,Thelen MP

    更新日期:1999-07-01 00:00:00

  • Expression and refolding of bioactive α-bungarotoxin V31 in E. coli.

    abstract::In order to obtain bioactive α-bungarotoxin (αBtx) using recombinant protein technique, a codon-optimized synthetic gene was expressed in fusion with the N-terminal 10-His-tag and C-terminal Strep-tag in Escherichia coli. Further optimization through site-directed mutagenesis enabled moderate expression of the protein...

    journal_title:Protein expression and purification

    pub_type: 杂志文章


    authors: Xu J,Li J,Wu X,Song C,Lin Y,Shen Y,Ye W,Sun C,Wang X,Li Z,Liu Y,Wei L,Li Z,Xu Z

    更新日期:2015-06-01 00:00:00

  • Cloning, expression and functional characterization of two sesquiterpene synthase genes from moso bamboo (Phyllostachys edulis).

    abstract::The purpose of this work was to characterize the functions of two sesquiterpene synthase genes from moso bamboo (Phyllostachys edulis). Two novel sesquiterpene synthase genes, belonging to the Tpsa subfamily, were isolated from moso bamboo. MoTPS2 was 1641 bp in length and encoded a protein of 63 kDa, whereas MoTPS6 w...

    journal_title:Protein expression and purification

    pub_type: 杂志文章


    authors: Chen X,Wang Y,Sun J,Wang J,Xun H,Tang F

    更新日期:2016-04-01 00:00:00

  • Overexpression and reconstitution of a Rieske iron-sulfur protein from the higher plant.

    abstract::The iron-sulfur protein subunit, known as the Rieske protein, is one of the central components of the cytochrome b(6)f complex residing in chloroplast and cyanobacterial thylakoid membranes. We have constructed plasmids for overexpression in Escherichia coli of full-length and truncated Rieske (PetC) proteins from the...

    journal_title:Protein expression and purification

    pub_type: 杂志文章


    authors: Gubernator B,Seidler A,Rögner M,Szczepaniak A

    更新日期:2003-05-01 00:00:00

  • Expression of active human GRO beta and GRO gamma neutrophil chemotactic proteins in E. coli.

    abstract::Human GRO alpha, GRO beta, and GRO gamma are neutrophil chemoattractants structurally related to IL-8 and compete with IL-8 for binding to IL-8 receptors on neutrophils. These proteins are part of a large superfamily of chemotactic cytokines, the "chemokines," members of which share striking structural similarities. W...

    journal_title:Protein expression and purification

    pub_type: 杂志文章


    authors: Zagorski J,DeLarco JE

    更新日期:1994-08-01 00:00:00

  • High-level expression of pseudolysin, the extracellular elastase of Pseudomonas aeruginosa, in Escherichia coli and its purification.

    abstract::Pseudolysin is the extracellular elastase of Pseudomonas aeruginosa and belongs to the thermolysin-like family of metallopeptidases. Pseudolysin has been identified as a robust drug target and a biotechnologically important enzyme in the tanning industry. Previous attempts to purify active pseudolysin from P. aerugino...

    journal_title:Protein expression and purification

    pub_type: 杂志文章


    authors: Odunuga OO,Adekoya OA,Sylte I

    更新日期:2015-09-01 00:00:00

  • Expression in Escherichia coli of the elongation factor 1beta gene and its nucleotide T160C mutant from the archaeon Sulfolobus solfataricus.

    abstract::The guanine nucleotide exchange factor EF-1beta gene from the thermoacidophilic archaeon Sulfolobus solfataricus (SsEF-1beta) was amplified by PCR and cloned into the pT7-7 expression vector. One of four selected clones harbored the T160C nucleotide substitution leading to the Y54H amino acid change in a hydrophobic r...

    journal_title:Protein expression and purification

    pub_type: 杂志文章


    authors: Ianniciello G,Masullo M,Raimo G,Arcari P,Bocchini V

    更新日期:1998-02-01 00:00:00

  • A screening strategy for heterologous protein expression in Escherichia coli with the highest return of investment.

    abstract::Heterologous protein expression in Escherichia coli is commonly used to obtain recombinant proteins for a variety of downstream applications. However, many proteins are not, or are only poorly, expressed in soluble form. High level expression often leads to the formation of inclusion bodies and an inactive product tha...

    journal_title:Protein expression and purification

    pub_type: 杂志文章


    authors: Pacheco B,Crombet L,Loppnau P,Cossar D

    更新日期:2012-01-01 00:00:00

  • High-level synthesis of recombinant murine endostatin in Chinese hamster ovary cells.

    abstract::Endostatin, a carboxy-terminal fragment of collagen XVIII, has been shown to act as an anti-angiogenic agent that specifically inhibits proliferation of endothelial cells and growth of various primary tumors. Here, we describe the expression by Chinese hamster ovary (CHO) cells of murine endostatin and of a tagged-fus...

    journal_title:Protein expression and purification

    pub_type: 杂志文章


    authors: Chura-Chambi RM,Tornieri PH,Spencer PJ,Nascimento PA,Mathor MB,Morganti L

    更新日期:2004-05-01 00:00:00

  • Production of recombinant human lactoferrin in the allantoic fluid of embryonated chicken eggs and its characteristics.

    abstract::The human iron-binding protein lactoferrin (hLf) has been implicated in a number of important physiological pathways, including those regulating immune function and tumor growth. In an effort to develop an efficient system for production of recombinant hLf (rhLf) that is structurally and functionally equivalent to the...

    journal_title:Protein expression and purification

    pub_type: 杂志文章


    authors: Tutykhina IL,Bezborodova OA,Shmarov MM,Logunov DY,Neugodova GL,Nemtsova ER,Naroditsky BS,Yakubovskaya RI,Gintsburg AL

    更新日期:2009-05-01 00:00:00

  • On-resin cleavage of bacterially expressed fusion proteins for purification of active recombinant peptides SK-29, KR-20, LL-29, and LL-23 from human sweat or skin.

    abstract::Post-translational processing of host defense cathelicidin peptide LL-37 in human sweat and skin generates new antimicrobial peptides. To understand structure and mechanism of action of these LL-37 derivatives, this article presents the cloning and expression of SK-29, KR-20, LL-29, and LL-23. We also provide a two-st...

    journal_title:Protein expression and purification

    pub_type: 杂志文章


    authors: Li Y,Li X,Wang G

    更新日期:2007-10-01 00:00:00

  • Expression of soluble, biologically active recombinant human endostatin in Escherichia coli.

    abstract::Endostatin, a 20kDa C-terminal fragment of collagen XVIII, is a potent anti-angiogenic protein and inhibitor of tumor growth. Recombinant endostatin was prepared from Escherichia coli deposited as insoluble, inactive inclusion bodies. In the present study, we produced soluble and biologically active recombinant human ...

    journal_title:Protein expression and purification

    pub_type: 杂志文章


    authors: Xu HM,Zhang GY,Ji XD,Cao L,Shu L,Hua ZC

    更新日期:2005-06-01 00:00:00

  • Improvement of extracellular secretion efficiency of Bacillus naganoensis pullulanase from recombinant Escherichia coli: Peptide fusion and cell wall modification.

    abstract::Pullulanases are well-known starch-debranching enzymes that are widely used for hydrolysis of a-1,6-glycosidic linkages in starch, pullulan, amylopectin, and other oligosaccharides. Escherichia coli is a popular heterologous expression host for generating target enzymes. However, cells have to be disrupted to obtain t...

    journal_title:Protein expression and purification

    pub_type: 杂志文章


    authors: Wang X,Chen Y,Nie Y,Xu Y

    更新日期:2019-03-01 00:00:00

  • Preventing protein aggregation by its hyper-acidic fusion cognates in Escherichia coli.

    abstract::Preventing protein aggregation is crucial for various protein studies, and has a large potential for remedy of protein misfolding or aggregates-linked diseases. In this study, we demonstrated the hyper-acidic protein fusion partners, which were previously reported to enhance the soluble expression of aggregation-prone...

    journal_title:Protein expression and purification

    pub_type: 杂志文章


    authors: Zou Z,Fan Y,Zhang C

    更新日期:2011-11-01 00:00:00

  • Unusual stability of manganese superoxide dismutase from a new species, Tatumella ptyseos ct: its gene structure, expression, and enzyme properties.

    abstract::A genomic DNA of 1416 bp containing an open reading frame encoding a manganese superoxide dismutase (Mn-SOD) from Tatumella ptyseos ct was cloned. Sequence analysis of this new gene revealed that it translates 205 amino acid residues. The deduced amino acid sequence showed variable identities (41-91%) with sequences o...

    journal_title:Protein expression and purification

    pub_type: 杂志文章


    authors: Ken CF,Lee CC,Duan KJ,Lin CT

    更新日期:2005-03-01 00:00:00

  • Periplasmic expression optimization of VEGFR2 D3 adopting response surface methodology: antiangiogenic activity study.

    abstract::Vascular endothelial growth factor (VEGF) is one of the most significant mediators of angiogenesis, which interacts with a specific membrane receptor: VEGF receptor 2 (VEGFR2). Studies elsewhere have shown that, a VEGF-blocker can regulate several vital processes of tumor promotion. However, there is no literature evi...

    journal_title:Protein expression and purification

    pub_type: 杂志文章


    authors: Cao W,Li H,Zhang J,Li D,Acheampong DO,Chen Z,Wang M

    更新日期:2013-08-01 00:00:00

  • High-throughput proteomics: protein expression and purification in the postgenomic world.

    abstract::Proteomics has become a major focus as researchers attempt to understand the vast amount of genomic information. Protein complexity makes identifying and understanding gene function inherently difficult. The challenge of studying proteins in a global way is driving the development of new technologies for systematic an...

    journal_title:Protein expression and purification

    pub_type: 杂志文章,评审


    authors: Lesley SA

    更新日期:2001-07-01 00:00:00

  • Increased peptide deformylase activity for N-formylmethionine processing of proteins overexpressed in Escherichia coli: application to homogeneous rubredoxin production.

    abstract::Deformylation of the initiator N-formylmethionine does not always proceed to completion for proteins overexpressed in Escherichia coli. To overcome this limitation, the def gene encoding the Escherichia coli peptide deformylase was cloned into the plysS plasmid under the tetracycline (Tc) promoter control. The efficie...

    journal_title:Protein expression and purification

    pub_type: 杂志文章


    authors: Tang J,Hernández G,LeMaster DM

    更新日期:2004-07-01 00:00:00

  • Expression of bioactive soluble human stem cell factor (SCF) from recombinant Escherichia coli by coproduction of thioredoxin and efficient purification using arginine in affinity chromatography.

    abstract::Stem cell factor (SCF) known as the c-kit ligand is a two disulfide bridge-containing cytokine in the regulation of the development and function of hematopoietic cell lineages and other cells such as mast cells, germ cells, and melanocytes. The secreted soluble form of SCF exists as noncovalently associated homodimer ...

    journal_title:Protein expression and purification

    pub_type: 杂志文章


    authors: Akuta T,Kikuchi-Ueda T,Imaizumi K,Oshikane H,Nakaki T,Okada Y,Sultana S,Kobayashi K,Kiyokawa N,Ono Y

    更新日期:2015-01-01 00:00:00

  • Requirement of continuous transcription for the synthesis of sufficient amounts of protein by a cell-free rapid translation system.

    abstract::To understand the key processes of cell-free protein synthesis, the synthesis of adipose-type fatty acid binding protein (A-FABP) by a rapid translation system was examined under various conditions. The synthesis of A-FABP was achieved by using an expression vector of A-FABP containing a T7 promoter. However, synthesi...

    journal_title:Protein expression and purification

    pub_type: 杂志文章


    authors: Hino M,Shinohara Y,Kajimoto K,Terada H,Baba Y

    更新日期:2002-03-01 00:00:00

  • Formation of well-defined soluble aggregates upon fusion to MBP is a generic property of E6 proteins from various human papillomavirus species.

    abstract::Protein aggregation is a main barrier hindering structural and functional studies of a number of interesting biological targets. The E6 oncoprotein of Human Papillomavirus strain 16 (E6(16)) is difficult to express under a native soluble form in bacteria. Produced as an unfused sequence, it forms inclusion bodies. Fus...

    journal_title:Protein expression and purification

    pub_type: 杂志文章


    authors: Zanier K,Nominé Y,Charbonnier S,Ruhlmann C,Schultz P,Schweizer J,Travé G

    更新日期:2007-01-01 00:00:00

  • High-cell density shake-flask expression and rapid purification of the large fragment of Thermus aquaticus DNA polymerase I using a new chemically and temperature inducible expression plasmid in Escherichia coli.

    abstract::We have developed a new expression vector, pcI(ts) ind(+), based upon the powerful rightward promoter of bacteriophage lambda, which is controlled by a temperature-sensitive and chemically-inducible version of the lambda repressor on the same plasmid. Locating the repressor gene on the plasmid makes this vector "porta...

    journal_title:Protein expression and purification

    pub_type: 杂志文章


    authors: Brandis JW,Johnson KA

    更新日期:2009-02-01 00:00:00

  • Expression and purification of a putative H-NS nucleoid-associated protein from the phytopathogen Xylella fastidiosa.

    abstract::The H-NS protein is one of the major constituents of the nucleoid structure that has been implicated in the DNA packaging and in the global regulation of gene expression. The study of this transcriptional regulator is an effort to fight Xylella fastidiosa, a citrus pathogen responsible for a range of economically impo...

    journal_title:Protein expression and purification

    pub_type: 杂志文章


    authors: Paula DP,Azzoni AR,Siqueira SF,Catani CF,Rosselli LK,de Souza AP

    更新日期:2003-11-01 00:00:00

  • Production and purification of the heavy-chain fragment C of botulinum neurotoxin, serotype B, expressed in the methylotrophic yeast Pichia pastoris.

    abstract::A recombinant Hc fragment of botulinum neurotoxin, serotype B (rBoNTB(Hc)), has been successfully expressed in a Mut+ strain of the methylotrophic yeast Pichia pastoris for use as an antigen in a proposed human vaccine. The fermentation process consisted of batch phase on glycerol, followed by glycerol and methanol fe...

    journal_title:Protein expression and purification

    pub_type: 杂志文章


    authors: Potter KJ,Bevins MA,Vassilieva EV,Chiruvolu VR,Smith T,Smith LA,Meagher MM

    更新日期:1998-08-01 00:00:00

  • Molecular cloning, expression, and functional analysis of the copper amine oxidase gene in the endophytic fungus Shiraia sp. Slf14 from Huperzia serrata.

    abstract::Huperzine A (HupA) is a drug used for the treatment of Alzheimer's disease. However, the biosynthesis of this medicinally important compound is not well understood. The HupA biosynthetic pathway is thought to be initiated by the decarboxylation of lysine to form cadaverine, which is then converted to 5-aminopentanal b...

    journal_title:Protein expression and purification

    pub_type: 杂志文章


    authors: Yang H,Peng S,Zhang Z,Yan R,Wang Y,Zhan J,Zhu D

    更新日期:2016-12-01 00:00:00

  • Xenopus phospho-CDK7/cyclin H expressed in baculoviral-infected insect cells.

    abstract::The cyclin-dependent kinase-activating kinase (CAK) catalyzes the phosphorylation of the cyclin-dependent protein kinases (CDKs) on a threonine residue (Thr160 in human CDK2). The reaction is an obligatory step in the activation of the CDKs. In higher eukaryotes, the CAK complex has been characterized in two forms. Th...

    journal_title:Protein expression and purification

    pub_type: 杂志文章


    authors: Lawrie AM,Tito P,Hernandez H,Brown NR,Robinson CV,Endicott JA,Noble ME,Johnson LN

    更新日期:2001-11-01 00:00:00

  • Cloning, expression, and purification of the Staphylococcus simulans lysostaphin using the intein-chitin-binding domain (CBD) system.

    abstract::The Staphylococcus simulans gene encoding lysostaphin has been PCR amplified from pRG5 recombinant plasmid (ATCC 67076) and cloned into Escherichia coli expression pTYB12 vector (IMPACT-CN System, New England BioLabs) which allows the overexpression of a target protein as a fusion to a self-cleavable affinity tag. The...

    journal_title:Protein expression and purification

    pub_type: 杂志文章


    authors: Szweda P,Pladzyk R,Kotlowski R,Kur J

    更新日期:2001-08-01 00:00:00

  • Statistical approaches to maximize recombinant protein expression in Escherichia coli: a general review.

    abstract::The supply of many valuable proteins that have potential clinical or industrial use is often limited by their low natural availability. With the modern advances in genomics, proteomics and bioinformatics, the number of proteins being produced using recombinant techniques is exponentially increasing and seems to guaran...

    journal_title:Protein expression and purification

    pub_type: 杂志文章,评审


    authors: Papaneophytou CP,Kontopidis G

    更新日期:2014-02-01 00:00:00

  • Purification and characterization of recombinant Plasmodium falciparum adenylosuccinate synthetase expressed in Escherichia coli.

    abstract::Most parasitic protozoa lack the de novo purine biosynthetic pathway and rely exclusively on the salvage pathway for their purine nucleotide requirements. Enzymes of the salvage pathway are, therefore, candidate drug targets. We have cloned the Plasmodium falciparum adenylosuccinate synthetase gene. In the parasite, a...

    journal_title:Protein expression and purification

    pub_type: 杂志文章


    authors: Jayalakshmi R,Sumathy K,Balaram H

    更新日期:2002-06-01 00:00:00

  • Production enhancement and refolding of caprine growth hormone expressed in Escherichia coli.

    abstract::This study describes comparison between IPTG and lactose induction on expression of caprine growth hormone (cGH), enhancing cell densities of Escherichia coli cultures and refolding the recombinant cGH, produced as inclusion bodies, to biologically active state. 2-3 times higher cell densities were obtained in shake f...

    journal_title:Protein expression and purification

    pub_type: 杂志文章


    authors: Khan MA,Sadaf S,Sajjad M,Waheed Akhtar M

    更新日期:2009-11-01 00:00:00