EcoliOverExpressionDB: a database of recombinant protein overexpression in E. coli.

abstract：:The cofactor heme (Fe-protoporphyrin IX) plays many important roles in biology. Identification of novel proteins for the transport, chaperoning and delivery of heme in cells is of widespread interest. Here, we describe the use of heme conjugated magnetic beads for the isolation of heme-binding proteins from complex pr...

journal_title：Protein expression and purification

authors： Jones R,Allen JW

更新日期：2011-03-01 00:00:00

abstract：:Apolipoprotein A-I (apoA-I) serves critical functions in plasma lipoprotein metabolism as a structural component of high density lipoprotein, activator of lecithin:cholesterol acyltransferase, and acceptor of cellular cholesterol as part of the reverse cholesterol transport pathway. In an effort to facilitate structur...

journal_title：Protein expression and purification

authors： Ryan RO,Forte TM,Oda MN

更新日期：2003-01-01 00:00:00

abstract：:An antifungal protein with a chitinase-like N-terminal sequence, designated delandin, was isolated from the rice bean. The protein exhibited a molecular weight of 28 kDa and was adsorbed on both blue Affi-Gel and SP-Toyopearl. It exerted antifungal action toward Mycosphaerella arachidicola, Botrytis cinerea, Fu- sariu...

journal_title：Protein expression and purification

authors： Ye XY,Ng TB

更新日期：2002-04-01 00:00:00

abstract：:Pokeweed antiviral protein (PAP)-I from the spring leaves of Phytolacca americana is a naturally occurring RNA-depurinating enzyme with broad-spectrum antiviral activity. Interest in PAP is growing due to its use as a potential anti-HIV agent. However, the clinical use of native PAP is limited due to inherent difficul...

journal_title：Protein expression and purification

authors： Rajamohan F,Doumbia SO,Engstrom CR,Pendergras SL,Maher DL,Uckun FM

更新日期：2000-03-01 00:00:00

abstract：:The UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase from Escherichia coli, an enzyme involved in the biosynthesis of the bacterial peptidoglycan monomer unit, was overproduced and purified to homogeneity on a large scale, yielding 4 mg of protein per liter of bacterial culture. Crystals of the complex with the subst...

journal_title：Protein expression and purification

authors： Auger G,Martin L,Bertrand J,Ferrari P,Fanchon E,Vaganay S,Pétillot Y,van Heijenoort J,Blanot D,Dideberg O

更新日期：1998-06-01 00:00:00

abstract：:Molecular-level investigation of proteins is increasingly important to researchers trying to understand the mechanisms of signal transmission. Heterotrimeric G proteins control the activation of many critical signal transmission cascades and are also implicated in numerous diseases. As part of a longer-term investigat...

journal_title：Protein expression and purification

authors： Maly J,Crowhurst KA

更新日期：2012-08-01 00:00:00

abstract：:Escherichia coli glutamic acid decarboxylase is a pyridoxal phosphate-dependent enzyme that catalyzes the alpha-decarboxylation of glutamate to yield 4-amino-butyrate and CO2. The E. coli chromosome contains two genes encoding for this enzyme, gadA and gadB, which map at distinct loci. Their protein products differ in...

journal_title：Protein expression and purification

authors： De Biase D,Tramonti A,John RA,Bossa F

更新日期：1996-12-01 00:00:00

abstract：:An Escherichia coli expression system that exploits the bacterial alkaline phosphatase (PhoA) signal sequence to translocate recombinant human epidermal growth factor (hEGF) to the periplasm was used to evaluate how changes in the composition and sequence of amino acids near the PhoA-hEGF junction influence the peripl...

journal_title：Protein expression and purification

authors： Campion SR,Elsasser E,Chung R

更新日期：1997-08-01 00:00:00

abstract：:The human PRODH gene has been shown to have unique roles in regulating cell survival and apoptotic pathways and it has been related to velocardiofacial syndrome/DiGeorge syndrome and increased susceptibility to schizophrenia. It encodes for the flavoprotein proline oxidase (PO), which catalyzes the conversion of l-pro...

journal_title：Protein expression and purification

authors： Tallarita E,Pollegioni L,Servi S,Molla G

更新日期：2012-04-01 00:00:00

abstract：:Stromal-cell-derived factor-1 (SDF-1alpha) is an 8-kDa chemokine that is constitutively expressed in bone-marrow-derived stromal cells and has been identified as a ligand for the CXCR4 receptor. We produced the chemokine recombinantly as methionine-SDF-1alpha in Escherichia coli without the leader peptide sequence. Th...

journal_title：Protein expression and purification

authors： Holmes WD,Consler TG,Dallas WS,Rocque WJ,Willard DH

更新日期：2001-04-01 00:00:00

abstract：:Separation of copurifying protease activity from recombinant human Müllerian inhibiting substance (rhMIS) bound to a monoclonal antibody immunoaffinity column by a high-salt wash results in cleaner preparations of rhMIS resistant to cleavage upon storage. In addition, an inhibitor of rhMIS antiproliferative activity i...

journal_title：Protein expression and purification

authors： Ragin RC,Donahoe PK,Kenneally MK,Ahmad MF,MacLaughlin DT

更新日期：1992-06-01 00:00:00

abstract：:Der p 2, a major allergen derived from the house dust mite Dermatophagoides pteronyssinus, is one of the most clinically relevant allergens worldwide. Recombinant Der p 2 (rDer p 2) is useful in clinical diagnosis and disease-specific immunotherapy. However, previous studies showed that Der p 2 can only be expressed i...

journal_title：Protein expression and purification

authors： Cao T,Zhang Z,Liu ZG,Dou X,Zhang J,Zhang W,Wu B,Yu ZD,Wei Z,Yu B

更新日期：2016-05-01 00:00:00

abstract：:A new method for the purification of manganese superoxide dismutase from human liver is described. The procedure involves essentially three steps: DEAE-cellulose, hydroxylapatite, and butyl-Toyopearl chromatographies. The method has several advantages: (i) its simplicity and rapidity (it takes less than 3 days), (ii) ...

journal_title：Protein expression and purification

authors： Matsuda Y,Suzuki K,Ookawara T,Nakata T,Seo HG,Kawata S,Tarui S,Deutsch HF,Taniguchi N

更新日期：1991-04-01 00:00:00

abstract：:Human GRO alpha, GRO beta, and GRO gamma are neutrophil chemoattractants structurally related to IL-8 and compete with IL-8 for binding to IL-8 receptors on neutrophils. These proteins are part of a large superfamily of chemotactic cytokines, the "chemokines," members of which share striking structural similarities. W...

journal_title：Protein expression and purification

authors： Zagorski J,DeLarco JE

更新日期：1994-08-01 00:00:00

abstract：:Trichoderma reesei tyrosinase TYR2 has been demonstrated to be able to oxidize various phenolic compounds and also peptide and protein bound tyrosine, and thus is of great interest for different biotechnological applications. In order to understand the reaction mechanism of the enzyme it would be essential to solve it...

journal_title：Protein expression and purification

authors： Westerholm-Parvinen A,Selinheimo E,Boer H,Kalkkinen N,Mattinen M,Saloheimo M

更新日期：2007-09-01 00:00:00

abstract：:The gram-positive bacterium Corynebacterium pseudotuberculosis is the causative agent of different diseases that cause dramatically reduced yields of wool and milk, and results in weight loss, carcass condemnation and also death mainly in sheep, equids, cattle and goats and therefore globally results in considerable e...

journal_title：Protein expression and purification

authors： Lindae A,Eberle RJ,Caruso IP,Coronado MA,de Moraes FR,Azevedo V,Arni RK

更新日期：2015-08-01 00:00:00

abstract：:Amyloid-beta (Aβ) peptide mediates several neurodegenerative diseases. The 42 amino acid (Aβ1-42) is the predominant form of peptide found in the neuritic plaques and has been demonstrated to be neurotoxic in vivo and in vitro. The availability of large quantities of Aβ peptide will help in several biochemical and bio...

journal_title：Protein expression and purification

authors： Chhetri G,Pandey T,Chinta R,Kumar A,Tripathi T

更新日期：2015-10-01 00:00:00

abstract：:Plasmids pQE-60 and pQE-30 containing 6 x His-tag sequence were used for expression of fragments of HCV structural and non-structural proteins in Escherichia coli (E. coli). The following fragments were used: core (1-98 aa), NS3 (202-482 aa), and tetramer of hypervariable region 1 (HVR1) of E2 protein. The constructed...

journal_title：Protein expression and purification

authors： Mihailova M,Fiedler M,Boos M,Petrovskis I,Sominskaya I,Roggendorf M,Viazov S,Pumpens P

更新日期：2006-11-01 00:00:00

abstract：:Insoluble expression of intrinsically soluble proteins with native activity is potentially a promising alternative to soluble expression of folded protein or insoluble expression of unfolded protein requiring refolding. Here, we attempted to express highly soluble halophilic His-rich metal binding protein (HP) as inso...

journal_title：Protein expression and purification

authors： Tokunaga M,Arakawa T,Tokunaga Y,Sugimoto Y,Ishibashi M

更新日期：2019-04-01 00:00:00

abstract：:Acyl carrier protein (ACP) was purified from Euglena gracilis variety bacillaris in yields of about 1 mg/100 g (wet wt) of cells. Antibodies against the purified protein were raised in hens and isolated from eggs. Antibodies raised against Euglena ACP inhibited the Euglena chloroplast nonaggregated fatty acid syntheta...

journal_title：Protein expression and purification

authors： Williams SG,Worsham LM,Ernst-Fonberg ML

更新日期：1991-04-01 00:00:00

abstract：:Fatty acid desaturases are enzymes that introduce double bonds into fatty acyl chains, among which stearoyl-acyl carrier protein desaturase (S-ACP-DES) was widely distributed in the plant kingdom. We cloned the cDNA coding for fab2/ssi2, an S-ACP-DES from Arabidopsis thaliana, into the vector pET30a and heterologously...

journal_title：Protein expression and purification

authors： Cao Y,Xian M,Yang J,Xu X,Liu W,Li L

更新日期：2010-02-01 00:00:00

abstract：:Homocysteine alpha,gamma-lyase from the anaerobic protozoan parasite Trichomonas vaginalis has been cloned from genomic DNA using PCR methods and expressed in Escherichia coli with a vector containing the T7 promoter. The recombinant homocysteine alpha,gamma-lyase (rHCYase) is expressed as the major protein in the hos...

journal_title：Protein expression and purification

authors： Han Q,Lenz M,Tan Y,Xu M,Sun X,Tan X,Tan X,Tang L,Miljkovic D,Hoffman RM

更新日期：1998-11-01 00:00:00

abstract：:The entire extracellular domain of the human heat-stable enterotoxin (ST) receptor as well as a truncated N-terminal domain were cloned as glutathione S-transferase fusion proteins and expressed in Escherichia coli. The recombinant fusion proteins were purified from both the cytosol and the inclusion body fractions by...

journal_title：Protein expression and purification

authors： Nandi A,Mathew R,Visweswariah SS

更新日期：1996-09-01 00:00:00

abstract：:The recombinant truncated endolysin LysK consisting of two catalytic domains, N-terminal CHAP and amidase-2 (LysKCA) was overexpressed in E. coli in the form of inclusion bodies (IBs). These IBs were dissolved in 6 M solution of urea followed by the refolding process. The refolding efficacy of the dilution and matrix-...

journal_title：Protein expression and purification

authors： Alaksandr Ž,Sergey G,Maksim P,Sergey K,Niyaz S,Uladzimir P,Mikhail S

更新日期：2020-10-01 00:00:00

abstract：:Anthrax toxin consists of three separate proteins, protective antigen (PA), lethal factor (LF), and edema factor (EF). PA binds to the receptor on mammalian cells and facilitates translocation of EF or LF into the cytosol. PA is the primary component of several anthrax vaccines. In this study we expressed and purified...

journal_title：Protein expression and purification

authors： Sharma M,Swain PK,Chopra AP,Chaudhary VK,Singh Y

更新日期：1996-02-01 00:00:00

abstract：:The entire stx2 region from Escherichia coli O157:H7, containing two open reading frames (stx2a and stx2b), was cloned into pET-32a with a single promoter, and transformed into E. coli BL21 (DE3) pLysS. We used two methods of IPTG induction using LB medium and auto-induction using ZYM-5052 medium to express recombinan...

journal_title：Protein expression and purification

authors： Tu W,Cai K,Gao X,Xiao L,Chen R,Shi J,Liu H,Hou X,Wang Q,Wang H

更新日期：2009-10-01 00:00:00

abstract：:A high-expression plasmid, pKSGHE3-1, containing the salt-tolerant glutaminase (EC 3.5.1.2) from marine bacterium Micrococcus luteus K-3 was constructed. pKSGHE3-1 was made by inserting the DNA fragment (1.43 kb) containing the structural gene synthesized by polymerase chain reaction into the downstream region of the ...

journal_title：Protein expression and purification

authors： Nandakumar R,Wakayama M,Nagano Y,Kawamura T,Sakai K,Moriguchi M

更新日期：1999-03-01 00:00:00

abstract：:Expression and purification of human beta-secretase (BACE1) in bacteria have been plagued with issues concerning solubility, inhomogeneous N-terminus, and lack of enzymic activity. Several forms of the mature human BACE1 have been expressed in Escherichia coli with different N-terminal extensions and without the C-ter...

journal_title：Protein expression and purification

authors： Sardana V,Xu B,Zugay-Murphy J,Chen Z,Sardana M,Darke PL,Munshi S,Kuo LC

更新日期：2004-04-01 00:00:00

abstract：:Mutant analogues of recombinant human immune interferon (IFN-gamma) with higher stability and biological activity were prepared. Depending on the analogue, protein structure modification might involve introduction of an intramonomer disulfide bond (through replacements of Glu7Cys and Ser69Cys), C-terminal shortening b...

journal_title：Protein expression and purification

authors： Pechenov SE,Tikhonov RV,Shingarova LN,Korobko VG,Yakimov SA,Klyushnichenko VE,Babajantz AA,Beliaev DL,Kuznetzov VP,Shvetz VI,Wulfson AN

更新日期：2002-03-01 00:00:00

abstract：:A full-length cDNA sequence of plant type CRY (designated Hae-P-CRY) was cloned from the green alga Haematococcus pluvialis. The cDNA sequence was 3608 base pairs (bp) in length, which contained a 2988-bp open reading frame encoding 995 amino acids with molecular mass of 107.7 kDa and isoelectric point of 6.19. Multip...

journal_title：Protein expression and purification

authors： Hang W,Gujar A,Zhang H,Xu W,Zhao C,Zhu X,Xue J,Zhang C,Ji C,Qin S,Li R,Cui H

更新日期：2020-08-01 00:00:00