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Isolation, purification and characterization of a novel solvent stable lipase from Pseudomonas reinekei.

Abstract：

:The Pseudomonas sp. have been long recognized for their exogenous lipolytic activities yet the genus still contains a lot of unexplored strains. Due to the versatile metabolic machinery and their potential for adaptation to fluctuating environmental conditions Pseudomonas sp. are of great interest for biotechnological applications. In this study, a new extracellularly produced lipolytic enzyme from Pseudomonas sp. (P. reinekei) was purified and characterized. The production of lipase from P. reinekei (H1) was enhanced 10-fold by optimizing the nitrogen source. The 50 kDa H1 lipase was purified using negative and positive mode anion exchange chromatography. The purified lipase was active over a broad pH range (5.0-9.0) and was stable for 24 h at 40 °C. The lipase showed significant stability, and indeed activation, in the presence of organic solvents with log P ≥ 2.0. These features render this lipase of interest as a biocatalyst for applications such as biodiesel production, detergent formulations and biodegradation of oil in the environment.

journal_name

Protein Expr Purif

journal_title

Protein expression and purification

authors

Priyanka P,Kinsella G,Henehan GT,Ryan BJ

doi

10.1016/j.pep.2018.08.007

subject

Has Abstract

pub_date

2019-01-01 00:00:00

pages

121-130

eissn

1046-5928

issn

1096-0279

pii

S1046-5928(18)30349-8

journal_volume

153

pub_type

杂志文章

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