Human cell lines: A promising alternative for recombinant FIX production.

abstract：:The coding region for arginyl-tRNA synthetase from jack bean (Canavalia ensiformis) has been sequenced and cloned into the bacterial expression vector pET32a. Transformation of BL21 cells and induction with IPTG results in the high level expression of the protein fused N-terminally with thioredoxin and bearing a His-t...

journal_title：Protein expression and purification

authors： Hogg J,Schiefermayr E,Schiltz E,Igloi GL

更新日期：2008-10-01 00:00:00

abstract：:The hexokinase (ATP;D-hexose 6-phosphotransferase, EC 2.7.1.1) of Schistosoma mansoni has been expressed in Escherichia coli as a fusion protein including an N-terminal polyhistidine tag and enterokinase cleavage site. The enzyme was purified by metal chelate chromatography to > 95% homogeneity, based on analysis by S...

journal_title：Protein expression and purification

authors： Armstrong RL,Wilson JE,Shoemaker CB

更新日期：1996-11-01 00:00:00

abstract：:Trichoderma reesei tyrosinase TYR2 has been demonstrated to be able to oxidize various phenolic compounds and also peptide and protein bound tyrosine, and thus is of great interest for different biotechnological applications. In order to understand the reaction mechanism of the enzyme it would be essential to solve it...

journal_title：Protein expression and purification

authors： Westerholm-Parvinen A,Selinheimo E,Boer H,Kalkkinen N,Mattinen M,Saloheimo M

更新日期：2007-09-01 00:00:00

abstract：:Virus-like particles (VLPs) of the recombinant hepatitis B virus (HBV) core protein (HBc) are routinely used in HBV diagnostics worldwide and are of potential interest as carriers of foreign peptides (e.g., immunological epitopes and targeting addresses, and/or as vessels for packaged diagnostic and therapeutic nanoma...

journal_title：Protein expression and purification

authors： Freivalds J,Dislers A,Ose V,Pumpens P,Tars K,Kazaks A

更新日期：2011-02-01 00:00:00

abstract：:Lactase deficiency problem discourages many adults from consuming milk as a major source of micro- and macronutrients. Enzymatic hydrolysis of lactose is an ideal solution for this problem but such processing adds significant costs. In this study, a cold active β-galactosidase from Planococcus sp-L4 (bgal) was optimiz...

journal_title：Protein expression and purification

authors： Mahdian SM,Karimi E,Tanipour MH,Parizadeh SM,Ghayour-Mobarhan M,Bazaz MM,Mashkani B

更新日期：2016-09-01 00:00:00

abstract：:In order to produce sufficient quantities of fibroblast growth factor-saporin (rFGF-2-SAP) mitotoxin for preclinical evaluation in models of diseases such as cancer and restenosis, we have undertaken the large-scale expression, purification, and characterization of the recombinant molecule. The fusion gene encoding rF...

journal_title：Protein expression and purification

authors： McDonald JR,Ong M,Shen C,Parandoosh Z,Sosnowski B,Bussell S,Houston LL

更新日期：1996-08-01 00:00:00

abstract：:To obtain strong bacterial expression of proteins that seem to be hard to express in bacteria or are highly toxic for bacteria, it is possible to create a palette of similar constructs, differing only by several nucleotides, gradually deleted from the full-length clone by exonuclease III. When a construct is equipped ...

journal_title：Protein expression and purification

authors： Pecenková T,Filigarová M,Cerovská N

更新日期：2005-05-01 00:00:00

abstract：:A cysteine-rich (approximately 20%), low molecular weight (MW 6 kDa) polypeptide has been isolated from the Korean blood-sucking leech, Hirudo nipponia. From its amino acid composition and N-terminal amino acid sequence analysis, the new protein is similar to granulin (or epithelin), and so it has been named leech gra...

journal_title：Protein expression and purification

authors： Hong SJ,Kang KW

更新日期：1999-07-01 00:00:00

abstract：:Rat granulocyte macrophage colony-stimulating factor (rGM-CSF) cDNA was amplified and cloned, and recombinant-rGM-CSF (R-rGM-CSF) was expressed and isolated from Escherichia coli. The synthesis of R-rGM-CSF was directed by a modified, inducible maltose binding protein (MBP) gene fusion expression vector, pMTR-23, and ...

journal_title：Protein expression and purification

authors： Holowachuk EW,Ruhoff MS

更新日期：1995-10-01 00:00:00

abstract：:Endostatin, a 20kDa C-terminal fragment of collagen XVIII, is a potent anti-angiogenic protein and inhibitor of tumor growth. Recombinant endostatin was prepared from Escherichia coli deposited as insoluble, inactive inclusion bodies. In the present study, we produced soluble and biologically active recombinant human ...

journal_title：Protein expression and purification

authors： Xu HM,Zhang GY,Ji XD,Cao L,Shu L,Hua ZC

更新日期：2005-06-01 00:00:00

abstract：:Heparinase I (HepA) was originally isolated from Flavobacterium heparinum (F. heparinum) and specifically cleaves heparin/heparan sulfate in a site-dependent manner, showing great promise for producing low molecular weight heparin (LMWH). However, expressing recombinant HepA is extremely difficult in Escherichia coli ...

journal_title：Protein expression and purification

authors： Huang J,Cao L,Guo W,Yuan R,Jia Z,Huang K

更新日期：2012-06-01 00:00:00

abstract：:Pigment epithelium-derived factor (PEDF) is a neurotrophic protein and a member of the serine protease inhibitor superfamily. Here we describe the identification of PEDF in bovine eyes and optimization of its purification from this natural source. We have developed a polyclonal antibody to recombinant human PEDF, Ab-r...

journal_title：Protein expression and purification

authors： Wu YQ,Notario V,Chader GJ,Becerra SP

更新日期：1995-08-01 00:00:00

abstract：:Interferon α-2a (IFNA2) is a member of the Type I interferon cytokine family, known for its antiviral and anti-proliferative functions. The role of this family in the innate immune response makes it an attractive candidate for the treatment of many viral and chronic immune-compromised diseases. Recombinant IFNA2 is cl...

journal_title：Protein expression and purification

authors： Bis RL,Stauffer TM,Singh SM,Lavoie TB,Mallela KM

更新日期：2014-07-01 00:00:00

abstract：:Hydrophobic interaction high-performance liquid chromatography (HIC-HPLC) was utilized for the separation of native human antithrombin (AT) and a partially denaturated form of AT, known as the latent form (L-AT). The AT used in this study is commercially available (Atenativ, Pharmacia & Upjohn, Sweden) and contains al...

journal_title：Protein expression and purification

authors： Karlsson G,Winge S

更新日期：2001-02-01 00:00:00

abstract：:The A(280)/A(260) ratio of a purified protein is frequently used as an indication of the purity of the preparation with respect to nucleic acids. We show here that for low-molecular-weight recombinant proteins purified from Escherichia coli, a low A(280)/A(260) ratio can also result from contamination with UDP-linked ...

journal_title：Protein expression and purification

authors： Ram MK,Andrade LJ,Phillips TB,van Schravendijk MR

更新日期：1999-11-01 00:00:00

abstract：:Cytosine-specific DNA methyltransferases are important enzymes in most living organisms. In prokaryotes, most DNA methyltransferases are members of the type II restriction-modification system where they methylate host DNA, thereby protecting it from digestion by the accompanying restriction endonucleases. DNA methyltr...

journal_title：Protein expression and purification

authors： Grgic M,Williamson A,Kjæreng Bjerga GE,Altermark B,Leiros I

更新日期：2018-10-01 00:00:00

abstract：:The XRCC1 DNA repair protein contains two regions of approximately 100 amino acids each that share homology with the BRCT (BRCA1 carboxyl terminus) domain superfamily. These two regions of XRCC1 have been shown to interact independently with DNA ligase III and poly(ADP-ribose)polymerase as part of a mechanism involved...

journal_title：Protein expression and purification

authors： Thornton K,Forstner M,Shen MR,West MG,Rupp B,Thelen MP

更新日期：1999-07-01 00:00:00

abstract：:The murine monoclonal antibody 125E11 is an IgG which recognizes PreS1(21-47) fragment of large hepatitis B surface antigen. It has been successfully used for clinical detection of HBV virion in serum of hepatitis B patients. In present study, the genes of variable region in heavy chain (VH) and light chain (VL) of 12...

journal_title：Protein expression and purification

authors： Yang X,Hu W,Li F,Xia H,Zhang Z

更新日期：2005-06-01 00:00:00

abstract：:The H-NS protein is one of the major constituents of the nucleoid structure that has been implicated in the DNA packaging and in the global regulation of gene expression. The study of this transcriptional regulator is an effort to fight Xylella fastidiosa, a citrus pathogen responsible for a range of economically impo...

journal_title：Protein expression and purification

authors： Paula DP,Azzoni AR,Siqueira SF,Catani CF,Rosselli LK,de Souza AP

更新日期：2003-11-01 00:00:00

abstract：:The role of the termini of protein sequences is often perturbed by remnant amino acids after the specific protease cleavage of the affinity tags and/or by the amino acids encoded by the plasmid at/around the restriction enzyme sites used to insert the genes. Here we describe a method for affinity purification of a met...

journal_title：Protein expression and purification

authors： Abd Elhameed HAH,Hajdu B,Balogh RK,Hermann E,Hunyadi-Gulyás É,Gyurcsik B

更新日期：2019-07-01 00:00:00

abstract：:Malassezia globosa is pathogenic fungus that causes skin disorders including dandruff in humans. Many yeast cytochrome CYP enzymes are involved in the biosynthesis of sterols and are considered major targets of azole antifungal agents. Here, we report on the expression and characterization of the MGL_0310 gene product...

journal_title：Protein expression and purification

authors： Ohk SO,Park HG,Lee H,Kwon YJ,Kim BJ,Kim D,Chun YJ

更新日期：2015-10-01 00:00:00

abstract：:As an insect-selective neurotoxin, scorpion long-chain BjαIT is a promising prospect for insecticidal application; however, the difficulty of obtaining natural BjαIT represents the major obstacle preventing analysis of its insecticidal activity against agricultural insect pests. Here, we screened recombinant Pichia pa...

journal_title：Protein expression and purification

authors： Li H,Xia Y

更新日期：2018-12-01 00:00:00

abstract：:An alpha-amylase has been purified from the thermophilic fungus Scytalidium thermophilum. A ninefold purification was achieved in a single step using fluidized bed chromatography wherein alginate was used as the affinity matrix. There are at least two isoenzymes as shown by concanavalin A (Con A)-agarose column chroma...

journal_title：Protein expression and purification

authors： Roy I,Sastry MS,Johri BN,Gupta MN

更新日期：2000-11-01 00:00:00

abstract：:Recombinant expression of human membrane proteins in large quantities remains a major challenge. Expression host is an important variable to screen for high-level production of membrane proteins. Using the green fluorescent protein (GFP) as a reporter, we screened the expression of a human multi-pass membrane protein ...

journal_title：Protein expression and purification

authors： Cai H,Yao H,Li T,Tang Y,Li D

更新日期：2019-12-01 00:00:00

abstract：:Homocysteine alpha,gamma-lyase from the anaerobic protozoan parasite Trichomonas vaginalis has been cloned from genomic DNA using PCR methods and expressed in Escherichia coli with a vector containing the T7 promoter. The recombinant homocysteine alpha,gamma-lyase (rHCYase) is expressed as the major protein in the hos...

journal_title：Protein expression and purification

authors： Han Q,Lenz M,Tan Y,Xu M,Sun X,Tan X,Tan X,Tang L,Miljkovic D,Hoffman RM

更新日期：1998-11-01 00:00:00

abstract：:The gene for the extremely thermophilic and thermostable 5'-methylthioadenosine phosphorylase from the archaeon Sulfolobus solfataricus was expressed at a high level in Escherichia coli thus providing a basis for detailed structural and functional studies of the enzyme. The recombinant enzyme was purified to homogenei...

journal_title：Protein expression and purification

authors： Cacciapuoti G,Fusco S,Caiazzo N,Zappia V,Porcelli M

更新日期：1999-06-01 00:00:00

abstract：:Envelope glycoprotein E of the dengue virus, which plays a crucial role in its entry into host cells, has an immunogenic domain III (EIII, amino acids 297-394), which is capable of inducing neutralizing antibodies. However, mice immunized with EIII protein without adjuvant elicited low immune responses. To improve low...

journal_title：Protein expression and purification

authors： Kim TG,Kim MY,Yang MS

更新日期：2010-12-01 00:00:00

abstract：:The gene from Aeromonas veronii bv. sobria encoding the metallo-beta-lactamase ImiS was subcloned into pET-26b, and ImiS was over-expressed in BL21(DE3) Escherichia coli and purified using SP-Sepharose chromatography. This protocol yielded over 5 mg of ImiS per liter of growth culture under optimum conditions. The bio...

journal_title：Protein expression and purification

authors： Crawford PA,Sharma N,Chandrasekar S,Sigdel T,Walsh TR,Spencer J,Crowder MW

更新日期：2004-08-01 00:00:00

abstract：:Mammalian target of rapamycin (TOR) controls cell growth and metabolism in response to the availability of nutrients, growth factors, and the cellular energy status. Misregulation of TOR can result in a pathogenic increase or decrease in organ size and in cancer. TOR can be inhibited by binding of a complex of rapamyc...

journal_title：Protein expression and purification

authors： Dames SA

更新日期：2008-05-01 00:00:00

abstract：:Pullulanase is crucial to the specific hydrolysis of branch points in amylopectin and is generally employed as an important enzyme in the starch-processing industry. Recombinant Bacillus subtilis that employs an inducible promoter would be a suitable candidate for pullulanase expression because of its safety and contr...

journal_title：Protein expression and purification

authors： Deng Y,Nie Y,Zhang Y,Wang Y,Xu Y

更新日期：2018-08-01 00:00:00