Abstract:
:For affinity-chromatography-based purification of proteins that are prone to abnormal termination of translation or that may not be modified at their N-termini, affinity tags are needed which can be fused to the C-terminus. In this publication we describe that maltose binding protein (MBP) fused to the C-terminus of the plant photoreceptor phytochrome B allows purification of the fusion protein via amylose affinity chromatography. After overexpression in yeast a 125-fold enrichment could be achieved. The spectral properties of phytochrome B were not impaired by the fusion and purification. These results demonstrate that not only the widely used N-terminal fusions of MBP but also C-terminal fusions can be employed for protein purification.
journal_name
Protein Expr Purifjournal_title
Protein expression and purificationauthors
Hennig L,Schäfer Edoi
10.1006/prep.1998.0969subject
Has Abstractpub_date
1998-12-01 00:00:00pages
367-70issue
3eissn
1046-5928issn
1096-0279pii
S1046-5928(98)90969-Xjournal_volume
14pub_type
杂志文章abstract::To understand the key processes of cell-free protein synthesis, the synthesis of adipose-type fatty acid binding protein (A-FABP) by a rapid translation system was examined under various conditions. The synthesis of A-FABP was achieved by using an expression vector of A-FABP containing a T7 promoter. However, synthesi...
journal_title:Protein expression and purification
pub_type: 杂志文章
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更新日期:2002-03-01 00:00:00
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journal_title:Protein expression and purification
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journal_title:Protein expression and purification
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abstract::Insulin-like growth factor binding proteins (IGFBPs) display many functions in humans including regulation of the insulin-like growth factor (IGF) signaling pathway. The various roles of human IGFBPs make them attractive protein candidates in drug discovery. Structural and functional knowledge on human proteins with t...
journal_title:Protein expression and purification
pub_type: 杂志文章
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abstract::HtrA2 is an apoptosis-activating protein to enhance the apoptotic process by preventing the formation of the IAP-caspase complex, thus freeing caspase to trigger the apoptosis pathway. Here, we presented the full-length sequence of HtrA2 from the black tiger shrimp (PmHtrA2). The full-length PmHtrA2 transcript was 140...
journal_title:Protein expression and purification
pub_type: 杂志文章
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journal_title:Protein expression and purification
pub_type: 杂志文章
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journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1016/j.pep.2005.06.002
更新日期:2006-01-01 00:00:00
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journal_title:Protein expression and purification
pub_type: 杂志文章
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journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1006/prep.2001.1586
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journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1016/j.pep.2011.09.001
更新日期:2012-01-01 00:00:00
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journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1016/j.pep.2014.01.012
更新日期:2014-04-01 00:00:00
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journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1016/j.pep.2008.10.023
更新日期:2009-04-01 00:00:00
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journal_title:Protein expression and purification
pub_type: 杂志文章
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journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1016/j.pep.2006.05.010
更新日期:2006-10-01 00:00:00
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journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1006/prep.1998.0910
更新日期:1998-08-01 00:00:00
abstract::A genomic DNA of 1416 bp containing an open reading frame encoding a manganese superoxide dismutase (Mn-SOD) from Tatumella ptyseos ct was cloned. Sequence analysis of this new gene revealed that it translates 205 amino acid residues. The deduced amino acid sequence showed variable identities (41-91%) with sequences o...
journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1016/j.pep.2004.10.003
更新日期:2005-03-01 00:00:00
abstract::Peptide-based affinity tags are commonly used in recombinant production/purification of proteins, and are often preceded or followed by a protease recognition sequence to allow tag removal. We describe a rat monoclonal antibody 2H5 recognizing an undecapeptide tag called "eTev", which contains a recognition sequence f...
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pub_type: 杂志文章
doi:10.1016/j.pep.2018.03.004
更新日期:2018-07-01 00:00:00
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journal_title:Protein expression and purification
pub_type: 杂志文章
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journal_title:Protein expression and purification
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journal_title:Protein expression and purification
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journal_title:Protein expression and purification
pub_type: 杂志文章
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journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1016/j.pep.2008.08.009
更新日期:2009-01-01 00:00:00
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journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1016/j.pep.2008.03.016
更新日期:2008-06-01 00:00:00
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journal_title:Protein expression and purification
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更新日期:2019-04-01 00:00:00
abstract::The sequence of the gene from Nocardia sp. NRRL 5646 encoding GTP cyclohydrolase I (GCH), gch, and its adjacent regions was determined. The open reading frame of Nocardia gch contains 684 nucleotides, and the deduced amino acid sequence represents a protein of 227 amino acid residues with a calculated molecular mass o...
journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1016/j.pep.2004.02.008
更新日期:2004-06-01 00:00:00
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journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1016/j.pep.2008.08.004
更新日期:2008-12-01 00:00:00
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journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1016/j.pep.2007.02.015
更新日期:2007-07-01 00:00:00
abstract::Homocysteine alpha,gamma-lyase from the anaerobic protozoan parasite Trichomonas vaginalis has been cloned from genomic DNA using PCR methods and expressed in Escherichia coli with a vector containing the T7 promoter. The recombinant homocysteine alpha,gamma-lyase (rHCYase) is expressed as the major protein in the hos...
journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1006/prep.1998.0955
更新日期:1998-11-01 00:00:00
abstract::Molecular-level investigation of proteins is increasingly important to researchers trying to understand the mechanisms of signal transmission. Heterotrimeric G proteins control the activation of many critical signal transmission cascades and are also implicated in numerous diseases. As part of a longer-term investigat...
journal_title:Protein expression and purification
pub_type: 杂志文章
doi:10.1016/j.pep.2012.06.003
更新日期:2012-08-01 00:00:00