Abstract:
:Conkunitzin-S1 from the cone snail Conus striatus is the first member of a new neurotoxin family with a canonical Kunitz domain fold. Conk-S1 is 60 amino acids long and lacks one of the three conserved disulfide bonds typically found in Kunitz domain modules. It binds specifically to voltage activated potassium channels of the Shaker family. The peptide was expressed in insoluble form in fusion with an N-terminal intein. Refolding in the presence of glutathione followed by pH shift-induced cleavage of the fusion protein resulted in a functional toxin as demonstrated by voltage-clamp measurements.
journal_name
Protein Expr Purifjournal_title
Protein expression and purificationauthors
Bayrhuber M,Graf R,Ferber M,Zweckstetter M,Imperial J,Garrett JE,Olivera BM,Terlau H,Becker Sdoi
10.1016/j.pep.2006.01.019keywords:
subject
Has Abstractpub_date
2006-06-01 00:00:00pages
640-4issue
2eissn
1046-5928issn
1096-0279pii
S1046-5928(06)00039-8journal_volume
47pub_type
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