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Expression of Pisum sativum SAD polypeptides in production hosts and in planta: tetrameric organization of the protein.

Abstract：

:In Pisum sativum, the short-chain alcohol dehydrogenase-like protein (SAD) gene family consists of at least three members (SAD-A, -B, and -C). Expression of two of these genes (SAD-A and -C) in Escherichia coli or Pichia pastoris resulted in full-length soluble proteins. Purified SAD-A was used as antigen for antibody production in rabbits. With these antibodies the recombinant SAD-C protein (which was most highly expressed of the two isoforms) was shown to be a tetramer consisting of a dimer of dimers. The SAD genes are transiently expressed in plants by short exposures to ultraviolet-B radiation (UV-B), as judged by northern blotting. In turn, mRNA accumulation leads to formation of SAD protein in leaf and stem tissue upon prolonged UV-B irradiation.

journal_name

Protein Expr Purif

journal_title

Protein expression and purification

authors

Scherbak N,Brosché M,Ala-Häivälä A,Strid H,Ohrfelt A,Nilsson F,Strid A

doi

10.1016/j.pep.2008.09.004

subject

Has Abstract

pub_date

2009-01-01 00:00:00

pages

18-25

issue

1

eissn

1046-5928

issn

1096-0279

pii

S1046-5928(08)00230-1

journal_volume

63

pub_type

杂志文章

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