Cloning and structural analysis of Mycobacterium leprae serine hydroxymethyltransferase.

abstract：:Lactase deficiency problem discourages many adults from consuming milk as a major source of micro- and macronutrients. Enzymatic hydrolysis of lactose is an ideal solution for this problem but such processing adds significant costs. In this study, a cold active β-galactosidase from Planococcus sp-L4 (bgal) was optimiz...

journal_title：Protein expression and purification

authors： Mahdian SM,Karimi E,Tanipour MH,Parizadeh SM,Ghayour-Mobarhan M,Bazaz MM,Mashkani B

更新日期：2016-09-01 00:00:00

abstract：:A soluble N-terminal domain of the Escherichia coli adenylyl transferase (ATase) is responsible for deadenylylation activity of the intact enzyme. Previous studies of the deadenylylation activity have involved a fragment, AT-N423 (residues 1 to 423), which was extended by 17 amino acids to give AT-N440. This new domai...

journal_title：Protein expression and purification

authors： Xu Y,Wen D,Clancy P,Carr PD,Ollis DL,Vasudevan SG

更新日期：2004-03-01 00:00:00

abstract：:A stable mammalian cell line expressing highly active bovine interferon-gamma (BoIFN-γ) was generated using Flp recombinase-mediated integration. This recombinant 293 cell line (B1) efficiently secreted FLAG-tagged BoIFN-γ protein into the culture supernatant, as determined by ELISA and Western blot. The recombinant B...

journal_title：Protein expression and purification

authors： Xu Z,Shan F,Shan F,Meng C,Zhou X,Zhang X,Chen X,Jiao X

更新日期：2014-07-01 00:00:00

abstract：:The sequence and structure of the target protein exert a marked effect on its soluble expression in Escherichia coli. The effects of the mutation of an amylase isolated from Bacillus licheniformis (BLA) on its soluble expression in E. coli were investigated. A random mutation library of BLA was constructed to screen f...

journal_title：Protein expression and purification

authors： Wang P,Qin W,Xu J,Yan Y,Tian J,Wu N,Yao B

更新日期：2016-04-01 00:00:00

abstract：:A growing number of studies require the purification of multiple proteins simultaneously and the development of simple economical high-throughput purification methods is essential. We have tested the purification of two related proteins in a variety of conditions to benchmark the semi-automated affinity chromatography...

journal_title：Protein expression and purification

authors： McGraw J,Tatipelli VK,Feyijinmi O,Traore MC,Eangoor P,Lane S,Stollar EJ

更新日期：2014-04-01 00:00:00

abstract：:L1 is a human cell adhesion glycoprotein involved in the development of the central nervous system that comprises six immunoglobulin-like domains (Ig1-Ig6), five fibronectin-type III (FN1-FN5) domains, a single transmembrane region and a cytoplasmic domain. It contains 20 potential N-glycosylation sites and is heavily...

journal_title：Protein expression and purification

authors： Gouveia RM,Morais VA,Peixoto C,Sousa M,Regalla M,Alves PM,Costa J

更新日期：2007-03-01 00:00:00

abstract：:The catalase gene of Psychrobacter sp. T-3 was cloned, and the gene product (PktA) was overexpressed in Escherichia coli. The specific activity of the purified PktA was slightly lower than that of the native purified enzyme obtained from Psychrobacter sp. T-3. Spectrophotometric measurements of the purified enzymes su...

journal_title：Protein expression and purification

authors： Kimoto H,Matsuyama H,Yumoto I,Yoshimune K

更新日期：2008-06-01 00:00:00

abstract：:Periplasmic expression of recombinant proteins ensures the production of biologically active proteins in a correctly folded state with several key advantages. This research focused on the in-frame cloning of rhIL-15 in pET-20 (+) vector with pelB-leader sequence to direct the protein to the bacterial periplasm. The ta...

journal_title：Protein expression and purification

authors： Ahmed N,Afroze B,Abbas R,Khan MA,Akram M,Tahir S,Bakht S,Munir A,Shahid AA

更新日期：2021-01-01 00:00:00

abstract：:Recombinant baculoviruses have proved to be a very useful means to express many proteins over the last 20 years. Since their introduction, there have been a number of significant improvements that have simplified and speeded up the construction of baculoviruses. One of the most commonly used methods relies upon recomb...

journal_title：Protein expression and purification

authors： McCall EJ,Danielsson A,Hardern IM,Dartsch C,Hicks R,Wahlberg JM,Abbott WM

更新日期：2005-07-01 00:00:00

abstract：:Process development and optimization studies were performed in order to improve the purification process of (rhIFN-gamma). The objective was to generate material with higher purity and quantity. An in-process control screening was developed to obtain the optimal condition for column chromatographic purification by mea...

journal_title：Protein expression and purification

authors： Mohammadian-Mosaabadi J,Naderi-Manesh H,Maghsoudi N,Nassiri-Khalili MA,Masoumian MR,Malek-Sabet N

更新日期：2007-02-01 00:00:00

abstract：:The mRNA encoding the 51-kDa subunit of 6-phosphogluconate dehydrogenase (6PGDH) from sheep liver was reverse-transcribed and amplified. The resulting cDNA was reamplified in N-terminal and C-terminal segments and spliced to generate a full-length clone, and an internal cDNA fragment was also amplified. The full-lengt...

journal_title：Protein expression and purification

authors： Chooback L,Price NE,Karsten WE,Nelson J,Sundstrom P,Cook PF

更新日期：1998-07-01 00:00:00

abstract：:CD23, a 45-kDa type II membrane glycoprotein present on B cells, monocytes, and other human immune cells, is a low-affinity receptor for IgE. The extracellular region of the membrane-bound human CD23 is processed into at least four soluble (s) CD23 forms, with apparent molecular masses of 37, 33, 29, and 25 kDa. High ...

journal_title：Protein expression and purification

authors： Khandekar SS,Mayer RJ,Cusimano DM,Katchur SR,Appelbaum ER

更新日期：2001-07-01 00:00:00

abstract：:Human interleukin-7 (IL-7) is a member of the interleukin family. Numerous studies have demonstrated IL-7's effect on B- and T-cell development as well as its potential in various clinical applications. Previously, a study reported that IL-7 could be purified from inclusion bodies using a prokaryotic system, however, ...

journal_title：Protein expression and purification

authors： Luo Y,Kong X,Xu A,Jin S,Wu D

更新日期：2009-01-01 00:00:00

abstract：:An unknown d-tagatose 3-epimerase (DTE) containing a IoIE domain was identified and cloned from Escherichia coli. This gene was subcloned into the prokaryotic expression vector pET-15b, and induced by IPTG in E. coli BL21 expression system. Through His-select gel column purification and fast-protein liquid chromatogra...

journal_title：Protein expression and purification

authors： He X,Zhou X,Yang Z,Xu L,Yu Y,Jia L,Li G

更新日期：2015-10-01 00:00:00

abstract：:(S)-Hydroxynitrile lyase (Hnl) from the tropical rubber tree Hevea brasiliensis catalyzes the formation of (S)-cyanohydrins from hydrocyanic acid and aldehydes or ketones. This enzyme accepts aliphatic, aromatic, and heterocyclic carbonyl compounds as substrates and is therefore considered a potent biocatalyst for the...

journal_title：Protein expression and purification

authors： Hasslacher M,Schall M,Hayn M,Bona R,Rumbold K,Lückl J,Griengl H,Kohlwein SD,Schwab H

更新日期：1997-10-01 00:00:00

abstract：:Lactobacillus β-galactosidases are mostly heterodimeric proteins, which are encoded by the two overlapping genes, lacL and lacM, and produced in recombinant prokaryotic systems for higher yield. This is the first report on the expression of a heterodimeric β-galactosidase from Lactobacillus crispatus B470 in Pichia pa...

journal_title：Protein expression and purification

authors： Nie C,Liu B,Zhang Y,Zhao G,Fan X,Ning X,Zhang W

更新日期：2013-11-01 00:00:00

abstract：:Recombinant OSU VP4 protein, and outer capsid antigen of porcine rotavirus, was purified to a high level from the spent broth of baculovirus-infected Spodoptera frugiperda insect cells. Initial clarification of the broth with a 0-60% ammonium sulfate cut retained 93% of the total VP4. Q-sepharose ion exchange chromato...

journal_title：Protein expression and purification

authors： Juarbe-Osorio LG,Gorziglia M,Betenbaugh MJ

更新日期：1993-06-01 00:00:00

abstract：:Acyl carrier protein (ACP) was purified from Euglena gracilis variety bacillaris in yields of about 1 mg/100 g (wet wt) of cells. Antibodies against the purified protein were raised in hens and isolated from eggs. Antibodies raised against Euglena ACP inhibited the Euglena chloroplast nonaggregated fatty acid syntheta...

journal_title：Protein expression and purification

authors： Williams SG,Worsham LM,Ernst-Fonberg ML

更新日期：1991-04-01 00:00:00

abstract：:The main principles of higher-order protein oligomerization are elucidated by many structural and biophysical studies. An astonishing number of proteins self-associate to form dimers or higher-order quaternary structures which further interact with other biomolecules to elicit complex cellular responses. In this study...

journal_title：Protein expression and purification

authors： Pandhare A,Stuebler AG,Pirayesh E,Jansen M

更新日期：2019-01-01 00:00:00

abstract：:Cell extracts of Clostridium kluyveri grown on ethanol plus succinate contained a NAD(H) dependent 4-hydroxybutanoate dehydrogenase (EC 1.1.1.61) at 66 U/mg. This enzyme was purified 42-fold under anaerobic conditions to homogeneity. Heat treatment, ion exchange chromatography on DEAE-cellulose, nondenaturing polyacry...

journal_title：Protein expression and purification

authors： Wolff RA,Kenealy WR

更新日期：1995-04-01 00:00:00

abstract：:The covalent addition of 4-amino-4-deoxy-L-arabinose (L-Ara4N) groups to lipid A, which resides in the outer membranes of bacteria such as Salmonella typhimurium and Escherichia coli, is the final step in the polymyxin-resistance pathway in these organisms. This modification is catalyzed by the inner membrane protein ...

journal_title：Protein expression and purification

authors： Bretscher LE,Morrell MT,Funk AL,Klug CS

更新日期：2006-03-01 00:00:00

abstract：:Procedures are described for the preparation of highly purified thymidylate synthases from Escherichia coli and Bacillus subtilis. The yields in each case are quite high with about 350 mg of pure protein obtained from 1 liter of cells. Basically all that is required to obtain pure enzyme is an induction step from a hi...

journal_title：Protein expression and purification

authors： Changchien LM,Garibian A,Frasca V,Lobo A,Maley GF,Maley F

更新日期：2000-07-01 00:00:00

abstract：:The zoonotic Rift Valley fever virus affects livestock and humans in Africa and on the Arabian Peninsula. The economic impact of this pathogen due to livestock losses, as well as its relevance to public health, underscores the importance of developing effective and easily distributed vaccines. Vaccines that can be del...

journal_title：Protein expression and purification

authors： Kalbina I,Lagerqvist N,Moiane B,Ahlm C,Andersson S,Strid Å,Falk KI

更新日期：2016-11-01 00:00:00

abstract：:The transcription factor Sp1 is a regulator of TATA-less genes. It belongs to the Cys₂-His₂ zinc finger domain-containing family. A zebrafish cDNA encoding a peptide homologous to mammalian Sp1 was cloned and inserted into a pET43.1a vector and expressed in Escherichia coli Rosetta (DE3) cells as a Nus-His-tag fusion ...

journal_title：Protein expression and purification

authors： Lin CJ,Hsiao TH,Chung YS,Chang WN,Yeh TM,Chen BH,Fu TF

更新日期：2011-03-01 00:00:00

abstract：:G-protein coupled receptors (GPCRs) are seven transmembrane helical proteins involved in cell signaling and response. They are targets for many existing therapeutic agents, and numerous drug discovery efforts. Production of large quantities of these receptors for drug screening and structural biology remains challengi...

journal_title：Protein expression and purification

authors： Bane SE,Velasquez JE,Robinson AS

更新日期：2007-04-01 00:00:00

abstract：:Cyclohexanone monooxygenase (CMO) is a soluble flavoenzyme originally isolated from Acinetobacter spp. which carries out Baeyer-Villiger reactions with cyclic ketone substrates. In the present study we cloned the Acinetobacter CMO gene and modified it for facile purification from heterologous expression systems by inc...

journal_title：Protein expression and purification

authors： Cheesman MJ,Kneller MB,Kelly EJ,Thompson SJ,Yeung CK,Eaton DL,Rettie AE

更新日期：2001-02-01 00:00:00

abstract：:A recombinant H(C) fragment of botulinum neurotoxin, serotype A (rBoNTA(H(C))), has been successfully expressed in a Mut(+) strain of the methylotrophic yeast Pichia pastoris for use as an antigen in a proposed human vaccine. Fermentation employed glycerol batch, glycerol-fed batch, and methanol-fed batch phases to ac...

journal_title：Protein expression and purification

authors： Potter KJ,Zhang W,Smith LA,Meagher MM

更新日期：2000-08-01 00:00:00

abstract：:The full-length cDNA that encodes the hemolytic toxin Avt-I, with 226 amino acids, from the venomous sea anemone Actineria villosa has been cloned using the oligo-capping method. The cDNA contains 681bp open reading frame and its predicted amino acid sequences revealed that Avt-I was basic polypeptides without cystein...

journal_title：Protein expression and purification

authors： Uechi G,Toma H,Arakawa T,Sato Y

更新日期：2005-04-01 00:00:00

abstract：:Methionine aminopeptidases (MetAPs), ubiquitous enzymes that play an important role in nascent protein maturation, have been recognized as attractive targets for the development of drugs against pathogenic protozoa including Plasmodium spp. Here, we characterized partial biochemical properties of a type I MetAP of Pla...

journal_title：Protein expression and purification

authors： Kang JM,Ju JW,Kim JY,Ju HL,Lee J,Lee KH,Lee WJ,Sohn WM,Kim TS,Na BK

更新日期：2015-04-01 00:00:00

abstract：:The assembly pathway of the insect cell Spodoptera frugiperda (Sf-9) was engineered to include expression of the murine chaperone immunoglobulin heavy chain binding protein (BiP) using the baculovirus vector. The impact of BiP coexpression on the production and secretion of functional and soluble recombinant immunoglo...

journal_title：Protein expression and purification

authors： Hsu TA,Eiden JJ,Bourgarel P,Meo T,Betenbaugh MJ

更新日期：1994-12-01 00:00:00