当前位置： SCI文献检索 > PROTEIN EXPRESSION AND PURIFICATION期刊下所有文献 > Cloning, expression and purification of d-tagatose 3-epimerase gene from Escherichia coli JM109.

Cloning, expression and purification of d-tagatose 3-epimerase gene from Escherichia coli JM109.

Abstract：

:An unknown d-tagatose 3-epimerase (DTE) containing a IoIE domain was identified and cloned from Escherichia coli. This gene was subcloned into the prokaryotic expression vector pET-15b, and induced by IPTG in E. coli BL21 expression system. Through His-select gel column purification and fast-protein liquid chromatography, highly purified and stable DTE protein was produced. The molecular weight of the DTE protein was estimated to be 29.8kDa. The latest 83 DTE sequences from public database were selected and analyzed by molecular clustering, multi-sequence alignment. DTEs were roughly divided into five categories.

journal_name

Protein Expr Purif

journal_title

Protein expression and purification

authors

He X,Zhou X,Yang Z,Xu L,Yu Y,Jia L,Li G

doi

10.1016/j.pep.2015.06.015

subject

Has Abstract

pub_date

2015-10-01 00:00:00

pages

77-81

eissn

1046-5928

issn

1096-0279

pii

S1046-5928(15)30003-6

journal_volume

114

pub_type

杂志文章

相关文献

PROTEIN EXPRESSION AND PURIFICATION文献大全
  • Purification of the medium-chain/long-chain (COT/CPT) carnitine acyltransferase of rat liver microsomes.

    abstract：:A procedure for the purification of the rat liver microsomal carnitine octanoyltransferase (COT) that catalyzes the reversible formation of medium-chain and long-chain acylcarnitines from acyl-coenzyme A is described. The K0.5 for L-carnitine is 0.6 mM and the K0.5 for both decanoyl-CoA and palmitoyl-CoA is 0.6 microM...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/1046-5928(92)90059-6

    authors： Murthy MS,Bieber LL

    更新日期：1992-02-01 00:00:00

  • Expression of the Trichoderma reesei tyrosinase 2 in Pichia pastoris: isotopic labeling and physicochemical characterization.

    abstract：:Trichoderma reesei tyrosinase TYR2 has been demonstrated to be able to oxidize various phenolic compounds and also peptide and protein bound tyrosine, and thus is of great interest for different biotechnological applications. In order to understand the reaction mechanism of the enzyme it would be essential to solve it...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2007.04.014

    authors： Westerholm-Parvinen A,Selinheimo E,Boer H,Kalkkinen N,Mattinen M,Saloheimo M

    更新日期：2007-09-01 00:00:00

  • Bacterial expression of human kynurenine 3-monooxygenase: solubility, activity, purification.

    abstract：:Kynurenine 3-monooxygenase (KMO) is an enzyme central to the kynurenine pathway of tryptophan metabolism. KMO has been implicated as a therapeutic target in several disease states, including Huntington's disease. Recombinant human KMO protein production is challenging due to the presence of transmembrane domains, whic...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2013.11.015

    authors： Wilson K,Mole DJ,Binnie M,Homer NZ,Zheng X,Yard BA,Iredale JP,Auer M,Webster SP

    更新日期：2014-03-01 00:00:00

  • Kinetic characterization of recombinant human cystathionine beta-synthase purified from E. coli.

    abstract：:Cystathionine beta-synthase (CBS) catalyzes the pyridoxal-50-phosphate-dependent condensation of L-serine and L-homocysteine to form L-cystathionine in the first step of the transsulfuration pathway. Although effective expression systems for recombinant human CBS (hCBS) have been developed, they require multiple chrom...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2008.10.012

    authors： Belew MS,Quazi FI,Willmore WG,Aitken SM

    更新日期：2009-04-01 00:00:00

  • Cloning, expression, isotope labeling, and purification of human antimicrobial peptide LL-37 in Escherichia coli for NMR studies.

    abstract：:Antimicrobial peptide LL-37 plays an important role in human body's first line of defense against infection. To better understand the mechanism of action, it is critical to elucidate the three-dimensional structure of LL-37 in complex with bacterial membranes. We present a bacterial expression system that allows the i...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2005.10.022

    authors： Li Y,Li X,Wang G

    更新日期：2006-06-01 00:00:00

  • Cloning, expression, and characterization of a novel plant type cryptochrome gene from the green alga Haematococcus pluvialis.

    abstract：:A full-length cDNA sequence of plant type CRY (designated Hae-P-CRY) was cloned from the green alga Haematococcus pluvialis. The cDNA sequence was 3608 base pairs (bp) in length, which contained a 2988-bp open reading frame encoding 995 amino acids with molecular mass of 107.7 kDa and isoelectric point of 6.19. Multip...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2020.105633

    authors： Hang W,Gujar A,Zhang H,Xu W,Zhao C,Zhu X,Xue J,Zhang C,Ji C,Qin S,Li R,Cui H

    更新日期：2020-08-01 00:00:00

  • Improved production of holotoxin Stx2 with biological activities by using a single-promoter vector and an auto-induction expression system.

    abstract：:The entire stx2 region from Escherichia coli O157:H7, containing two open reading frames (stx2a and stx2b), was cloned into pET-32a with a single promoter, and transformed into E. coli BL21 (DE3) pLysS. We used two methods of IPTG induction using LB medium and auto-induction using ZYM-5052 medium to express recombinan...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2009.05.005

    authors： Tu W,Cai K,Gao X,Xiao L,Chen R,Shi J,Liu H,Hou X,Wang Q,Wang H

    更新日期：2009-10-01 00:00:00

  • A new method for the purification of bioactive insulin-like growth factor-binding protein-3.

    abstract：:We present a novel efficient procedure for high level purification of human IGFBP-3. Insulin-like growth factor-binding proteins (IGFBPs) are key regulators of insulin-like growth factor mediated signal transduction and thereby can profoundly influence cellular phenotypes. Certain IGFBPs, including IGFBP-3, have also ...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2010.02.005

    authors： Pircher H,Matscheski A,Laich A,Hermann M,Moser B,Viertler HP,Micutkova L,Lindner H,Sarg B,Zwerschke W,Jansen-Dürr P

    更新日期：2010-06-01 00:00:00

  • Binding studies using Pichia pastoris expressed human aryl hydrocarbon receptor and aryl hydrocarbon receptor nuclear translocator proteins.

    abstract：:The aryl hydrocarbon receptor (AHR) is a transcription factor which activates gene transcription by binding to its corresponding enhancer as the heterodimer, which is consisted of AHR and the aryl hydrocarbon receptor nuclear translocator (ARNT). Human AHR can be rather difficult to study, when compared among the AHR ...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2016.02.011

    authors： Zheng Y,Xie J,Huang X,Dong J,Park MS,Chan WK

    更新日期：2016-06-01 00:00:00

  • Synthesis of a human lysosomal enzyme, beta-hexosaminidase B, using the baculovirus expression system.

    abstract：:Human lysosomal beta-hexosaminidase exists in two major forms: the A isoform is composed of both alpha and beta chains, while the B form is a homopolymer of beta chains. Deficiency of beta-hexosaminidase underlies the GM2 gangliosidoses. We have produced active beta-hexosaminidase B in cultured insect (Sf9) cells by i...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/1046-5928(90)90003-h

    authors： Boose JA,Tifft CJ,Proia RL,Myerowitz R

    更新日期：1990-11-01 00:00:00

  • Construction, expression and purification of a novel CadF-based multiepitope antigen and its immunogenic polyclonal antibody specific to Campylobacter jejuni and Campylobacter coli.

    abstract：:Campylobacteriosis is a disease in humans caused by the infection from Campylobacter spp. Human cases are mainly due to Campylobacter jejuni, although C. coli can cause gastroenteritis in humans as well. The bacteria are commensal in chicken tract and can be contaminated into chicken products during processing. Obviou...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2021.105818

    authors： Wenbap P,Seetang-Nun Y,Luangtongkum T,Khunrae P,Tuitemwong P,Rattanarojpong T

    更新日期：2021-04-01 00:00:00

  • Cholera toxin B subunit-domain III of dengue virus envelope glycoprotein E fusion protein production in transgenic plants.

    abstract：:Envelope glycoprotein E of the dengue virus, which plays a crucial role in its entry into host cells, has an immunogenic domain III (EIII, amino acids 297-394), which is capable of inducing neutralizing antibodies. However, mice immunized with EIII protein without adjuvant elicited low immune responses. To improve low...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2010.07.013

    authors： Kim TG,Kim MY,Yang MS

    更新日期：2010-12-01 00:00:00

  • Cloning, expression and purification of a glycosylated form of the DNA-binding protein Dps from Salmonella enterica Typhimurium.

    abstract：:Dps, found in many eubacterial and archaebacterial species, appears to protect cells from oxidative stress and/or nutrient-limited environment. Dps has been shown to accumulate during the stationary phase, to bind to DNA non-specifically, and to form a crystalline structure that compacts and protects the chromosome. O...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2008.01.015

    authors： Hanna ES,Roque-Barreira MC,Mendes GM,Soares SG,Brocchi M

    更新日期：2008-06-01 00:00:00

  • Frameshift events associated with the lysyl-tRNA and the rare arginine codon, AGA, in Escherichia coli: a case study involving the human Relaxin 2 protein.

    abstract：:Human Relaxin 2 is an insulin-related peptide hormone with a mass of 19,084 Da. The mRNA contains a number of arginine codons that are rarely used by Escherichia coli to produce highly expressed proteins. As a result, expressing this recombinant protein in E. coli is problematic. When human Relaxin 2 was expressed in ...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2008.02.016

    authors： Kerrigan JJ,McNulty DE,Burns M,Allen KE,Tang X,Lu Q,Trulli JM,Johanson KO,Kane JF

    更新日期：2008-08-01 00:00:00

  • Improved solubility of replication factor C (RFC) Walker A mutants.

    abstract：:Protein insolubility often poses a significant problem during purification protocols and in enzyme assays, especially for eukaryotic proteins expressed in a recombinant bacterial system. The limited solubility of replication factor C (RFC), the clamp loader complex from Saccharomyces cerevisiae, has been previously do...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2012.03.010

    authors： Marzahn MR,Bloom LB

    更新日期：2012-06-01 00:00:00

  • EcoliOverExpressionDB: a database of recombinant protein overexpression in E. coli.

    abstract：UNLABELLED:Recombinant protein production is a significant biotechnological process as it allows researchers to produce a specific protein in desired quantities. Escherichia coli (E. coli) is the most popular heterologous expression host for the production of recombinant proteins due to its advantages such as low cost,...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2013.11.014

    authors： Habibi N,Samian MR,Hashim SZ,Norouzi A

    更新日期：2014-03-01 00:00:00

  • Affinity chromatographic purification of a protein which binds specifically to the yeast leucine tRNA gene.

    abstract：:A crude cell extract from yeast Saccharomyces cerevisae was fractionated by affinity chromatography using the leucine tRNA gene as the recognition site. This approach enables the rapid purification of a protein, which retained its full DNA binding capacity during the enrichment procedure. The active fraction contains ...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/1046-5928(90)90015-q

    authors： Kaufmann E

    更新日期：1990-11-01 00:00:00

  • Heterologous expression of stearoyl-acyl carrier protein desaturase (S-ACP-DES) from Arabidopsis thaliana in Escherichia coli.

    abstract：:Fatty acid desaturases are enzymes that introduce double bonds into fatty acyl chains, among which stearoyl-acyl carrier protein desaturase (S-ACP-DES) was widely distributed in the plant kingdom. We cloned the cDNA coding for fab2/ssi2, an S-ACP-DES from Arabidopsis thaliana, into the vector pET30a and heterologously...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2009.08.011

    authors： Cao Y,Xian M,Yang J,Xu X,Liu W,Li L

    更新日期：2010-02-01 00:00:00

  • Production and single-step purification of EGFP and a biotinylated version of the Human Rhinovirus 14 3C protease.

    abstract：:The fluorescent reporter enhanced Green Fluorescent Protein (EGFP) has been used for assaying a wide range of biological activities ranging from gene expression, or localization of target proteins through to intermolecular interactions. However, over-production of this protein in Escherichia coli has resulted in the p...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2011.05.003

    authors： Youell J,Fordham D,Firman K

    更新日期：2011-10-01 00:00:00

  • Generation and evaluation of mammalian secreted and membrane protein expression libraries for high-throughput target discovery.

    abstract：:Expressed protein libraries are becoming a critical tool for new target discovery in the pharmaceutical industry. In order to get the most meaningful and comprehensive results from protein library screens, it is essential to have library proteins in their native conformation with proper post-translation modifications....

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2011.05.011

    authors： Panavas T,Lu J,Liu X,Winkis AM,Powers G,Naso MF,Amegadzie B

    更新日期：2011-09-01 00:00:00

  • Expression, purification, and characterization of human malonyl-CoA decarboxylase.

    abstract：:The recombinant human malonyl-CoA decarboxylase (hMCD) was overexpressed in Escherichia coli with and without the first 39 N-terminal amino acids via a cleavable MBP-fusion construct. Proteolytic digestion using genenase I to remove the MBP-fusion tag was optimized for both the full length and truncated hMCD. The apo-...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2003.11.023

    authors： Zhou D,Yuen P,Chu D,Thon V,McConnell S,Brown S,Tsang A,Pena M,Russell A,Cheng JF,Nadzan AM,Barbosa MS,Dyck JR,Lopaschuk GD,Yang G

    更新日期：2004-04-01 00:00:00

  • Refolding and one-step purification of recombinant human ARA70 over-expressed in Escherichia coli.

    abstract：:Androgen receptor (AR)-associated coregulator 70 (ARA70) is a cytoplasmic protein that has been characterized to have the ability to induce AR transcriptional activity in response to androgens and anti-androgens in prostate cancer cells. AR has been shown to have an important role in the progression of prostate cancer...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2004.11.002

    authors： Singh VK,Jia Z

    更新日期：2005-02-01 00:00:00

  • Purification and characterization of recombinant forms of murine Tcl1 proteins.

    abstract：:The TCL1 gene, which is located on chromosome 14, plays a major role in human hematopoietic malignancies and encodes a 14-kDa protein whose function has not been determined. This gene is expressed in pre-B cells, in immature thymocytes, and, at low levels, in activated T cells but not in peripheral mature B cells and ...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1006/prep.1999.1186

    authors： Du Bois GC,Song SP,Kulikovskaya I,Rothstein JL,Germann MW,Croce CM

    更新日期：2000-04-01 00:00:00

  • Expression and purification of biologically active human granulocyte-macrophage colony stimulating factor (hGM-CSF) using silkworm-baculovirus expression vector system.

    abstract：:Human granulocyte-macrophage colony stimulating factor (hGM-CSF) is a hematopoietic growth factor. It is widely employed as a therapeutic agent targeting neutropenia in cancer patients undergoing chemotherapy and in patients with AIDS or after bone marrow transplantation. In this study, we constructed the recombinant ...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2019.03.010

    authors： Kinoshita Y,Xu J,Masuda A,Minamihata K,Kamiya N,Mon H,Fujita R,Kusakabe T,Lee JM

    更新日期：2019-07-01 00:00:00

  • Gene optimization is necessary to express a bivalent anti-human anti-T cell immunotoxin in Pichia pastoris.

    abstract：:The bivalent anti-human anti-T cell immunotoxin A-dmDT390-bisFv(G(4)S) was developed for treatment of T cell leukemia, autoimmune diseases, and tolerance induction for transplantation. The multi-domain structure of the bivalent immunotoxin hinders efficient production in Escherichia coli and most eukaryotes are sensit...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/s1046-5928(02)00009-8

    authors： Woo JH,Liu YY,Mathias A,Stavrou S,Wang Z,Thompson J,Neville DM Jr

    更新日期：2002-07-01 00:00:00

  • Efficient secretory expression of functional barley limit dextrinase inhibitor by high cell-density fermentation of Pichia pastoris.

    abstract：:The limit dextrinase inhibitor (LDI) from barley seeds acts specifically on limit dextrinase (LD), an endogenous starch debranching enzyme. LDI is a 14 kDa hydrophobic protein containing four disulfide bonds and one unpaired thiol group previously found to be either glutathionylated or cysteinylated. It is a member of...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2011.04.009

    authors： Jensen JM,Vester-Christensen MB,Møller MS,Bønsager BC,Christensen HE,Hachem MA,Svensson B

    更新日期：2011-10-01 00:00:00

  • An improved method for high-level soluble expression and purification of recombinant amyloid-beta peptide for in vitro studies.

    abstract：:Amyloid-beta (Aβ) peptide mediates several neurodegenerative diseases. The 42 amino acid (Aβ1-42) is the predominant form of peptide found in the neuritic plaques and has been demonstrated to be neurotoxic in vivo and in vitro. The availability of large quantities of Aβ peptide will help in several biochemical and bio...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2015.05.015

    authors： Chhetri G,Pandey T,Chinta R,Kumar A,Tripathi T

    更新日期：2015-10-01 00:00:00

  • Thermostable tyrosine phenol-lyase of Symbiobacterium sp. SC-1: gene cloning, sequence determination, and overproduction in Escherichia coli.

    abstract：:During the screening for tyrosine phenol-lyase-producing thermophiles, we isolated an obligatory symbiotic thermophile, Symbiobacterium sp. SC-1, which grew only in coculture with Bacillus sp. SK-1. A gene encoding thermostable tyrosine phenol-lyase (TPL) was cloned from the genomic DNA of the Symbiobacterium sp. SC-1...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1006/prep.1997.0792

    authors： Lee SG,Hong SP,Choi YH,Chung YJ,Sung MH

    更新日期：1997-12-01 00:00:00

  • Remedial strategies in structural proteomics: expression, purification, and crystallization of the Vav1/Rac1 complex.

    abstract：:The signal transduction pathway involving the Vav1 guanine nucleotide exchange factor (GEF) and the Rac1 GTPase plays several key roles in the immune response mediated by the T cell receptor. Vav1 is also a unique member of the GEF family in that it contains a cysteine-rich domain (CRD) that is critical for Rac1 bindi...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2006.10.027

    authors： Brooun A,Foster SA,Chrencik JE,Chien EY,Kolatkar AR,Streiff M,Ramage P,Widmer H,Weckbecker G,Kuhn P

    更新日期：2007-05-01 00:00:00

  • Expression, purification and preliminary characterization of glucagon receptor extracellular domain.

    abstract：:Glucagon is a pancreatic hormone that plays pivotal roles in regulating glucose homeostasis and metabolism. Glucagon exerts its action by binding to its receptor, glucagon receptor (GCGR), one of class B G-protein coupled receptors (GPCRs). Diabetes is a bihormonal disease in which excessive glucagon secretion is a ma...

    journal_title：Protein expression and purification

    pub_type： 杂志文章

    doi：10.1016/j.pep.2013.04.004

    authors： Wu L,Zhai Y,Lu J,Wang Q,Sun F

    更新日期：2013-06-01 00:00:00