Purification of functional human Cl(-)/HCO(3)(-) exchanger, AE1, over-expressed in Saccharomyces cerevisiae.

abstract：:The DEX gene encoding an extracellular dextranase from Lipomyces starkeyi was cloned into vector pPIC9k-His6 and was expressed in Pichia pastoris GS115 strain under the control of AOX1 promoter. After 107 h of the 5L-scaled fermentation, wet cells weight of the recombinant P. pastoris Mut(+) strain reached to 588.6g/L...

journal_title：Protein expression and purification

authors： Chen L,Zhou X,Fan W,Zhang Y

更新日期：2008-03-01 00:00:00

abstract：:Escherichia coli glutamic acid decarboxylase is a pyridoxal phosphate-dependent enzyme that catalyzes the alpha-decarboxylation of glutamate to yield 4-amino-butyrate and CO2. The E. coli chromosome contains two genes encoding for this enzyme, gadA and gadB, which map at distinct loci. Their protein products differ in...

journal_title：Protein expression and purification

authors： De Biase D,Tramonti A,John RA,Bossa F

更新日期：1996-12-01 00:00:00

abstract：:Expressed protein libraries are becoming a critical tool for new target discovery in the pharmaceutical industry. In order to get the most meaningful and comprehensive results from protein library screens, it is essential to have library proteins in their native conformation with proper post-translation modifications....

journal_title：Protein expression and purification

authors： Panavas T,Lu J,Liu X,Winkis AM,Powers G,Naso MF,Amegadzie B

更新日期：2011-09-01 00:00:00

abstract：:Lactobacillus β-galactosidases are mostly heterodimeric proteins, which are encoded by the two overlapping genes, lacL and lacM, and produced in recombinant prokaryotic systems for higher yield. This is the first report on the expression of a heterodimeric β-galactosidase from Lactobacillus crispatus B470 in Pichia pa...

journal_title：Protein expression and purification

authors： Nie C,Liu B,Zhang Y,Zhao G,Fan X,Ning X,Zhang W

更新日期：2013-11-01 00:00:00

abstract：:Enteropeptidase (synonym:enterokinase, EC 3.4.21.9) is a heterodimeric serine protease of the intestinal brush border that activates trypsinogen by highly specific cleavage of the trypsinogen activation peptide following the sequence (Asp)(4)-Lys. The DNA sequence encoding the light chain (catalytic subunit) of human ...

journal_title：Protein expression and purification

authors： Gasparian ME,Ostapchenko VG,Schulga AA,Dolgikh DA,Kirpichnikov MP

更新日期：2003-09-01 00:00:00

abstract：:A simple scheme for the rapid and efficient isolation of rat pro-atrial natriuretic factor (pro-ANF) has been developed. An isolated rat adrenal cell bioassay for ANF was established to optimize heart tissue extraction and chromatography conditions. This assay is based on the ability of ANF to inhibit angiotensin II-s...

journal_title：Protein expression and purification

authors： Belcourt D,Varma DR,Toney K,Bennett HP

更新日期：1990-09-01 00:00:00

abstract：:An unknown d-tagatose 3-epimerase (DTE) containing a IoIE domain was identified and cloned from Escherichia coli. This gene was subcloned into the prokaryotic expression vector pET-15b, and induced by IPTG in E. coli BL21 expression system. Through His-select gel column purification and fast-protein liquid chromatogra...

journal_title：Protein expression and purification

authors： He X,Zhou X,Yang Z,Xu L,Yu Y,Jia L,Li G

更新日期：2015-10-01 00:00:00

abstract：:We have cloned, from total human liver RNA, the cDNA encoding apolipoprotein E3 (apoE3). Site-directed mutagenesis was used to obtain the cDNA encoding the apoE4 isoform, a major variant of this apolipoprotein in man. These two cDNAs were subcloned into the procaryotic expression vector pAHRS. A polyhistidine tag was ...

journal_title：Protein expression and purification

authors： Pillot T,Barbier A,Visvikis A,Lozac'h K,Rosseneu M,Vandekerckhove J,Siest G

更新日期：1996-06-01 00:00:00

abstract：:Human prolactin was expressed in insect culture cells by recombinant baculoviruses carrying prolactin gene cDNA placed under the transcriptional control of polyhedrin gene promoter of Autographa californica nuclear polyhedrosis virus. Preliminary results of recombinant human prolactin expression as extracellular as we...

journal_title：Protein expression and purification

authors： Strokovskaya L,Bartoszewicz Z,Szolajska E,Kikhno I,Solomko A,Michalik J

更新日期：2001-07-01 00:00:00

abstract：:The role of the termini of protein sequences is often perturbed by remnant amino acids after the specific protease cleavage of the affinity tags and/or by the amino acids encoded by the plasmid at/around the restriction enzyme sites used to insert the genes. Here we describe a method for affinity purification of a met...

journal_title：Protein expression and purification

authors： Abd Elhameed HAH,Hajdu B,Balogh RK,Hermann E,Hunyadi-Gulyás É,Gyurcsik B

更新日期：2019-07-01 00:00:00

abstract：:Novel GSH-AP (phenoxyl agarose coated gigaporous polystyrene, Agap-co-PSt) microspheres were successfully prepared by introducing GSH ligand into hydrophilic AP microspheres pre-activated with 1,4-butanediol diglycidyl ether. The gigaporous structure and chromatographic properties of GSH-AP medium were evaluated and c...

journal_title：Protein expression and purification

authors： Huang Y,Zhang R,Li J,Li Q,Su Z,Ma G

更新日期：2014-03-01 00:00:00

abstract：:Botulinum neurotoxin light chain (BoNT LC, 50 kDa) is responsible for the zinc endopeptidase activity specific for proteins of neuroexocytosis apparatus. We describe the expression of recombinant type A BoNT LC in Escherichia coli as well as the purification and characterization of the recombinant protein. A high leve...

journal_title：Protein expression and purification

authors： Li L,Singh BR

更新日期：1999-12-01 00:00:00

abstract：:The introduction of an affinity tag offers an attractive approach to isolation of membrane proteins. The type of affinity tag and its positioning in the protein is determined by the desired subsequent experimental uses of the isolated protein. To minimize the risk of interference, membrane proteins may preferentially ...

journal_title：Protein expression and purification

authors： Hamann T,Laursen T,Møller BL

更新日期：2009-11-01 00:00:00

abstract：:An alpha-amylase has been purified from the thermophilic fungus Scytalidium thermophilum. A ninefold purification was achieved in a single step using fluidized bed chromatography wherein alginate was used as the affinity matrix. There are at least two isoenzymes as shown by concanavalin A (Con A)-agarose column chroma...

journal_title：Protein expression and purification

authors： Roy I,Sastry MS,Johri BN,Gupta MN

更新日期：2000-11-01 00:00:00

abstract：:l-glutamate oxidase (GLOD), encoded by the gox gene, catalyses the transformation of l-glutamic acid into α-ketoglutaric acid (α-KG). In the present study, Pichia pastoris was used for heterologous production of GLOD following optimization of the gox coding sequence for expression in the yeast host. A series of constr...

journal_title：Protein expression and purification

authors： YaPing W,Ben R,Hong Y,Rui H,Li L,Ping'an L,Lixin M

更新日期：2017-01-01 00:00:00

abstract：:The iron-sulfur protein subunit, known as the Rieske protein, is one of the central components of the cytochrome b(6)f complex residing in chloroplast and cyanobacterial thylakoid membranes. We have constructed plasmids for overexpression in Escherichia coli of full-length and truncated Rieske (PetC) proteins from the...

journal_title：Protein expression and purification

authors： Gubernator B,Seidler A,Rögner M,Szczepaniak A

更新日期：2003-05-01 00:00:00

abstract：:Telomerase is a specialized reverse transcriptase that catalyzes the addition of telomeric repeats, TTAGGG in all vertebrates, to the ends of chromosomes. The lack of recombinant purified human telomerase reverse transcriptase (hTERT) has hampered biochemical and structural studies. The primary problem in generating a...

journal_title：Protein expression and purification

authors： Wu CK,Gousset K,Hughes SH

更新日期：2007-11-01 00:00:00

abstract：:Ketol-acid reductoisomerase (EC 1.1.1.86) catalyzes the conversion of 2-aceto-2-hydroxyacids to 2-keto-3-hydroxyacids and their subsequent reduction by NADPH to 2,3-dihydroxyacids. The gene encoding the Escherichia coli enzyme was cloned and expressed as a hexahistidine-tagged fusion protein and the recombinant enzyme...

journal_title：Protein expression and purification

authors： Hill CM,Duggleby RG

更新日期：1999-02-01 00:00:00

abstract：:Large amounts of soluble fibronectin were easily purified from cryoprecipitated or fresh citrated human blood plasma by a three-step combination of gelatin and heparin-cellufine affinity chromatography. The elution conditions were optimized to obtain a homogeneous fraction on SDS-PAGE and Western blot under reducing c...

journal_title：Protein expression and purification

authors： Poulouin L,Gallet O,Rouahi M,Imhoff JM

更新日期：1999-10-01 00:00:00

abstract：:To facilitate studies of multicomponent protein complexes, I have developed an Escherichia coli expression system which coexpresses up to four polypeptides from a single plasmid. The modular nature of the system enables efficient subcloning of a gene into each of the 4 cassettes in the polycistronic expression vector....

journal_title：Protein expression and purification

authors： Tan S

更新日期：2001-02-01 00:00:00

abstract：:Chinese hamster dihydrofolate reductase (ch-DHFR) was overexpressed in Escherichia coli DH5 alpha under the transcriptional control of PRPL promoters regulated by temperature-sensitive repressors. The desired recombinant product is soluble and constitutes about 30% of the total soluble proteins of the bacterial cell. ...

journal_title：Protein expression and purification

authors： Fan YX,Wang B,Jing GZ

更新日期：1997-03-01 00:00:00

abstract：:Recombinant murine MRP14 (mMRP14) was produced in Escherichia coli using the pGEX expression system. The mass of fusion protein, by electrospray ionization-mass spectrometry (ESI/MS), was 39,213 Da which compares well with the theoretical mass (39,210.4 Da). Thrombin digestion of fusion protein was expected at a clone...

journal_title：Protein expression and purification

authors： Raftery MJ,Collinson L,Geczy CL

更新日期：1999-03-01 00:00:00

abstract：:In this report, we describe an optimized system for the efficient overexpression, purification, and refolding of secreted bacterial proteins. Candidate secreted proteins were produced recombinantly in Escherichia coli as Tobacco Etch Virus protease-cleavable hexahistidine-c-myc eptiope fusion proteins. Without regard ...

journal_title：Protein expression and purification

authors： Geisbrecht BV,Bouyain S,Pop M

更新日期：2006-03-01 00:00:00

abstract：:Endostatin, a carboxy-terminal fragment of collagen XVIII, has been shown to act as an anti-angiogenic agent that specifically inhibits proliferation of endothelial cells and growth of various primary tumors. Here, we describe the expression by Chinese hamster ovary (CHO) cells of murine endostatin and of a tagged-fus...

journal_title：Protein expression and purification

authors： Chura-Chambi RM,Tornieri PH,Spencer PJ,Nascimento PA,Mathor MB,Morganti L

更新日期：2004-05-01 00:00:00

abstract：:Endo-1,4-β-mannanase is an enzyme that can catalyze the random hydrolysis of β-1,4-mannosidic linkages in the main chain of mannans, glucomannans and galactomannans and offers many applications in different biotechnology industries. Purification and kinetic properties of the endo-1,4-β-mannanase from recombinant Esche...

journal_title：Protein expression and purification

authors： Tuntrakool P,Keawsompong S

更新日期：2018-06-01 00:00:00

abstract：:Human lysosomal beta-hexosaminidase exists in two major forms: the A isoform is composed of both alpha and beta chains, while the B form is a homopolymer of beta chains. Deficiency of beta-hexosaminidase underlies the GM2 gangliosidoses. We have produced active beta-hexosaminidase B in cultured insect (Sf9) cells by i...

journal_title：Protein expression and purification

authors： Boose JA,Tifft CJ,Proia RL,Myerowitz R

更新日期：1990-11-01 00:00:00

abstract：:An efficient expression system for a low-molecular mass xylanase in Escherichia coli has been developed. A gene encoding the mature Bacillus circulans (Bc) xylanase was designed to imitate the frequency of degenerate codons used in E. coli. Appropriate degenerate codons were used to create multiple unique restriction ...

journal_title：Protein expression and purification

authors： Sung WL,Luk CK,Zahab DM,Wakarchuk W

更新日期：1993-06-01 00:00:00

abstract：:Apolipoprotein A-I (apoA-I) serves critical functions in plasma lipoprotein metabolism as a structural component of high density lipoprotein, activator of lecithin:cholesterol acyltransferase, and acceptor of cellular cholesterol as part of the reverse cholesterol transport pathway. In an effort to facilitate structur...

journal_title：Protein expression and purification

authors： Ryan RO,Forte TM,Oda MN

更新日期：2003-01-01 00:00:00

abstract：:To understand the key processes of cell-free protein synthesis, the synthesis of adipose-type fatty acid binding protein (A-FABP) by a rapid translation system was examined under various conditions. The synthesis of A-FABP was achieved by using an expression vector of A-FABP containing a T7 promoter. However, synthesi...

journal_title：Protein expression and purification

authors： Hino M,Shinohara Y,Kajimoto K,Terada H,Baba Y

更新日期：2002-03-01 00:00:00

abstract：:Vibriolysin, an extracellular protease of Vibrio proteolyticus, is synthesized as a preproenzyme. The N-terminal propeptide functions as an intramolecular chaperone and an inhibitor of the mature enzyme. Extracellular production of recombinant vibriolysin has been achieved in Bacillus subtilis, but not in Escherichia ...

journal_title：Protein expression and purification

authors： Sonoda H,Sugimura A

更新日期：2008-12-01 00:00:00