Overexpression of the Bacillus subtilis and circulans xylanases in Escherichia coli.

abstract：:A tremendous increase has taken place over the last decades in the biochemical and clinical use of antibodies. Unfortunately, the constantly growing demand has not been matched by a corresponding easy access to pure immunoglobulin, as purification procedures tend to be either laborious, expensive, or inefficient. We p...

journal_title：Protein expression and purification

authors： Osmark P,Cedervall T,Pieters K,Akerström B

更新日期：2003-08-01 00:00:00

abstract：:Human granulocyte-macrophage colony-stimulating factor (GM-CSF), a hemopoietic growth factor, was produced and secreted from tobacco cell suspensions. The GM-CSF cDNA was carried by a binary vector under the control of the CaMV 35S promoter and the T7 terminator. In addition, a 5'-nontranslated region from the tobacco...

journal_title：Protein expression and purification

authors： James EA,Wang C,Wang Z,Reeves R,Shin JH,Magnuson NS,Lee JM

更新日期：2000-06-01 00:00:00

abstract：:Human granulocyte-macrophage colony stimulating factor (hGM-CSF) is a hematopoietic growth factor. It is widely employed as a therapeutic agent targeting neutropenia in cancer patients undergoing chemotherapy and in patients with AIDS or after bone marrow transplantation. In this study, we constructed the recombinant ...

journal_title：Protein expression and purification

authors： Kinoshita Y,Xu J,Masuda A,Minamihata K,Kamiya N,Mon H,Fujita R,Kusakabe T,Lee JM

更新日期：2019-07-01 00:00:00

abstract：:There is no high-resolution structure for the membrane domain of the human erythrocyte anion exchanger, AE1 (Band 3). In this report, we have developed an expression and purification strategy for AE1 to be used in crystallization trials. Saccharomyces cerevisiae strain BJ5457 was transformed with an expression vector ...

journal_title：Protein expression and purification

authors： Bonar P,Casey JR

更新日期：2010-11-01 00:00:00

abstract：:Most parasitic protozoa lack the de novo purine biosynthetic pathway and rely exclusively on the salvage pathway for their purine nucleotide requirements. Enzymes of the salvage pathway are, therefore, candidate drug targets. We have cloned the Plasmodium falciparum adenylosuccinate synthetase gene. In the parasite, a...

journal_title：Protein expression and purification

authors： Jayalakshmi R,Sumathy K,Balaram H

更新日期：2002-06-01 00:00:00

abstract：:A strategy for the purification and cleavage of chimeric recombinant proteins based on a genetically engineered metal-binding peptide and a human renin cleavage site is described. Vectors were constructed to direct the synthesis of chimeric human immunodeficiency virus (HIV) reverse transcriptase (RT) or beta-galactos...

journal_title：Protein expression and purification

authors： Evans DB,Tarpley WG,Sharma SK

更新日期：1991-04-01 00:00:00

abstract：:A recombinant Hc fragment of botulinum neurotoxin, serotype B (rBoNTB(Hc)), has been successfully expressed in a Mut+ strain of the methylotrophic yeast Pichia pastoris for use as an antigen in a proposed human vaccine. The fermentation process consisted of batch phase on glycerol, followed by glycerol and methanol fe...

journal_title：Protein expression and purification

authors： Potter KJ,Bevins MA,Vassilieva EV,Chiruvolu VR,Smith T,Smith LA,Meagher MM

更新日期：1998-08-01 00:00:00

abstract：:High-level constitutive expression of xylanase GH11 from Penicillium occitanis Pol6 termed PoXyn2 was achieved using the methylotrophic yeast Pichia pastoris. The PoXyn2 cDNA encoding for a mature xylanase of 320 amino acids was subcloned into the pGAPZαA vector, to construct recombinant xylanse with six histidine res...

journal_title：Protein expression and purification

authors： Driss D,Bhiri F,Ghorbel R,Chaabouni SE

更新日期：2012-05-01 00:00:00

abstract：:The second enzyme of the naphthalene degradation pathway in Pseudomonas putida G7 is NahB, a dehydrogenase that converts cis-1,2-dihydroxy-1,2-dihydronaphthalene to 1,2-dihydroxynaphthalene. We report the cloning, optimization of expression, purification, kinetic studies and preliminary structural characterization of ...

journal_title：Protein expression and purification

authors： Costa DMA,Costa MAF,Guimarães SL,Coitinho JB,Gómez SV,Brandão TADS,Nagem RAP

更新日期：2017-04-01 00:00:00

abstract：:Der p 2, a major allergen derived from the house dust mite Dermatophagoides pteronyssinus, is one of the most clinically relevant allergens worldwide. Recombinant Der p 2 (rDer p 2) is useful in clinical diagnosis and disease-specific immunotherapy. However, previous studies showed that Der p 2 can only be expressed i...

journal_title：Protein expression and purification

authors： Cao T,Zhang Z,Liu ZG,Dou X,Zhang J,Zhang W,Wu B,Yu ZD,Wei Z,Yu B

更新日期：2016-05-01 00:00:00

abstract：:The trematode Schistosoma mansoni Sm14 antigen was expressed in the yeast Pichia pastoris and secreted into the culture medium at yields of approximately 250 mg L-1. Sm14 belongs to a family of fatty-acid binding proteins and appears to play an important role in uptake, transport, and compartmentalization of lipids in...

journal_title：Protein expression and purification

authors： Damasceno L,Ritter G,Batt CA

更新日期：2017-06-01 00:00:00

abstract：:Trichoderma reesei tyrosinase TYR2 has been demonstrated to be able to oxidize various phenolic compounds and also peptide and protein bound tyrosine, and thus is of great interest for different biotechnological applications. In order to understand the reaction mechanism of the enzyme it would be essential to solve it...

journal_title：Protein expression and purification

authors： Westerholm-Parvinen A,Selinheimo E,Boer H,Kalkkinen N,Mattinen M,Saloheimo M

更新日期：2007-09-01 00:00:00

abstract：:Formation of biominerals often involves specific proteins that modulate the process of matrix assembly, nucleation, and crystal growth. AP7 is an aragonite-associated protein of 7 kDa and is intrinsically disordered. The structural disorder of AP7 makes it very difficult to express in Escherchiacoli. In this work, we ...

journal_title：Protein expression and purification

authors： Huang YC,Chang HH,Mou Y,Chi P,Chan JC,Luo SC

更新日期：2014-08-01 00:00:00

abstract：:Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), which is capable of selectively inducing apoptosis of cancer cells, is a potential targeted drug for cancer therapy. The TRAIL protein induces apoptosis only in trimeric form. However, the recombinant soluble TRAIL (sTRAIL) trimer has low stability and a...

journal_title：Protein expression and purification

authors： Wang B,Wang Z,Yan J,Wang L,Wang Z,Wu J,Zhang H,Wu H,Kong W,Yu B,Yu X

更新日期：2015-11-01 00:00:00

abstract：:Haloalkane dehalogenase from Xanthobacter autotrophicus was efficiently expressed in Escherichia coli BL21 (DE3) and E. coli JM101. After introduction of restriction sites by PCR the haloalkane dehalogenase gene (dhlA) was translationally fused behind the T7 (phi 10), trc, and tac promoters. This resulted in expressio...

journal_title：Protein expression and purification

authors： Schanstra JP,Rink R,Pries F,Janssen DB

更新日期：1993-10-01 00:00:00

abstract：:Ciliary neurotrophic factor (CNTF) is characterized as a neuropoietic cytokine for a broad spectrum of neurons, leading to its evaluation in humans suffering from neurodegenerative diseases. Due to its wide range of biological applications, high yield production of soluble biologically active recombinant human CNTF (r...

journal_title：Protein expression and purification

authors： Wang K,Zhou F,Zhu L,Zhu X,Zhang K,Zhu L

更新日期：2014-04-01 00:00:00

abstract：:Amidase signature (AS) family amidases are known to exhibit broad substrate specificity. According to the available genome sequence data, a novel AS family amidase, Pl-Ami, was identified and cloned from the genome of Parvibaculum lavamentivorans ZJB14001. The recombinant amidase was overexpressed in Escherichia coli ...

journal_title：Protein expression and purification

authors： Wu ZM,Zheng RC,Zheng YG

更新日期：2017-01-01 00:00:00

abstract：:Hsp47 is regarded as a collagen-specific chaperone with several suggested roles in collagen biosynthesis under normal and disease conditions. We describe here a procedure for the expression and purification of Hsp47 in Escherichia coli using the IMPACT expression system (New England Biolabs) where the guest gene is fu...

journal_title：Protein expression and purification

authors： Thomson CA,Ananthanarayanan VS

更新日期：2001-10-01 00:00:00

abstract：:The role of FIC (Filamentation induced by cAMP)(2) domain containing proteins in the regulation of many vital pathways, mostly through the transfer of NMPs from NTPs to specific target proteins (NMPylation), in microorganisms, higher eukaryotes, and plants is emerging. The identity and function of FIC domain containin...

journal_title：Protein expression and purification

authors： Mishra S,Bhagavat R,Chandra N,Vijayarangan N,Rajeswari H,Ajitkumar P

更新日期：2012-11-01 00:00:00

abstract：:Receptor activator of NF-kappaB ligand (RANKL) is a type II transmembrane protein found on osteoblasts which functions as a major determinant of osteoclast differentiation and activation. RANKL mediates bone homeostasis through binding to the cognate ligand on osteoclasts, RANK, and a soluble decoy receptor, osteoprot...

journal_title：Protein expression and purification

authors： Willard D,Chen WJ,Barrett G,Blackburn K,Bynum J,Consler T,Hoffman C,Horne E,Iannone MA,Kadwell S,Parham J,Ellis B

更新日期：2000-10-01 00:00:00

abstract：:Human small nuclear (sn) RNA genes are transcribed by either RNA polymerase II or III depending upon the arrangement of their core promoter elements. Regardless of polymerase specificity, these genes share a requirement for a general transcription factor called the snRNA activating protein complex or SNAP(C). This mul...

journal_title：Protein expression and purification

authors： Hanzlowsky A,Jelencic B,Jawdekar G,Hinkley CS,Geiger JH,Henry RW

更新日期：2006-08-01 00:00:00

abstract：:Reticulons are a large family of integral membrane proteins that are ubiquitous in eukaryotes and play a key role in functional remodelling of the endoplasmic reticulum membrane. The reticulon family is especially large in plants, with the Arabidopsis thaliana genome containing twenty-one isoforms. Reticulons vary in ...

journal_title：Protein expression and purification

authors： Chow M,Sklepari M,Frigerio L,Dixon AM

更新日期：2018-12-01 00:00:00

abstract：:(S)-Hydroxynitrile lyase (Hnl) from the tropical rubber tree Hevea brasiliensis catalyzes the formation of (S)-cyanohydrins from hydrocyanic acid and aldehydes or ketones. This enzyme accepts aliphatic, aromatic, and heterocyclic carbonyl compounds as substrates and is therefore considered a potent biocatalyst for the...

journal_title：Protein expression and purification

authors： Hasslacher M,Schall M,Hayn M,Bona R,Rumbold K,Lückl J,Griengl H,Kohlwein SD,Schwab H

更新日期：1997-10-01 00:00:00

abstract：:The human PRODH gene has been shown to have unique roles in regulating cell survival and apoptotic pathways and it has been related to velocardiofacial syndrome/DiGeorge syndrome and increased susceptibility to schizophrenia. It encodes for the flavoprotein proline oxidase (PO), which catalyzes the conversion of l-pro...

journal_title：Protein expression and purification

authors： Tallarita E,Pollegioni L,Servi S,Molla G

更新日期：2012-04-01 00:00:00

abstract：:Human paraoxonase (hPON3) is a high density lipoprotein-related glycoprotein with multi-enzymatic properties and antioxidant activity which is proposed to participate in the prevention of low density lipoprotein (LDL) oxidation. In this study, hPON3 gene was amplified from Human Fetal Liver Marathon-Ready cDNA and exp...

journal_title：Protein expression and purification

authors： Lu H,Zhu J,Zang Y,Ze Y,Qin J

更新日期：2006-03-01 00:00:00

abstract：:The erm proteins confer resistance to the MLS (macrolide-lincosamide-streptogramin B) antibiotics in various microorganisms, including pathogens, through dimethylation of a single adenine residue (A2085: Bacillus subtilis coordinate) of the 23S rRNA to reduce the affinity of antibiotics, thereby enabling the cells to ...

journal_title：Protein expression and purification

authors： Jin HJ,Yang YD

更新日期：2002-06-01 00:00:00

abstract：:Antibacterial peptides from various sources express different antibacterial activity. In order to obtain a high activity antibacterial peptide, the sequences of four antimicrobial peptides--Protegrin-1, 4 kDa Scorpion Defensin, Metalnikowin-2A and Sheep Myeloid Antibacterial Peptide SMAP-29--were exploited to generate...

journal_title：Protein expression and purification

authors： Niu M,Li X,Wei J,Cao R,Zhou B,Chen P

更新日期：2008-01-01 00:00:00

abstract：:Disulfide bonds are normally formed after a polypeptide has been exported from the reducing environment of the cytoplasm into a more oxidizing compartment, such as the bacterial periplasm. Recently, we showed that in Escherichia coli trxB gor mutants, in which the reduction of thioredoxin and glutathione is impaired, ...

journal_title：Protein expression and purification

authors： Levy R,Weiss R,Chen G,Iverson BL,Georgiou G

更新日期：2001-11-01 00:00:00

abstract：:High-level extracellular production of Fusarium solani cutinase was achieved using a Pichia pastoris expression system. The cutinase-encoding gene was cloned into pPICZalphaA with the Saccharomyces cerevisiae alpha-factor signal sequence and methanol-inducible alcohol oxidase promoter by two different ways. The additi...

journal_title：Protein expression and purification

authors： Kwon MA,Kim HS,Yang TH,Song BK,Song JK

更新日期：2009-11-01 00:00:00

abstract：:Advances in expressed protein ligation (EPL) methods that permit specific introduction of unique modifications into proteins have facilitated protein engineering, structure-function and protein interaction studies. An EPL-generated hybrid exchangeable apolipoprotein has been constructed from recombinant fragments of a...

journal_title：Protein expression and purification

authors： Hauser PS,Ryan RO

更新日期：2007-08-01 00:00:00