Reprint of: Immobilized-Metal Affinity Chromatography (IMAC): A Review.

abstract：:A cDNA clone, lambda GTHP1del, encoding glutathione transferase (GST) P1-1, was isolated from a human K562 erythroleukemia cell line cDNA library. The coding sequence was lacking the codons for the N-terminal 34 amino acids. A DNA segment was designed in order to obtain the missing portion and a structure representing...

journal_title：Protein expression and purification

authors： Kolm RH,Stenberg G,Widersten M,Mannervik B

更新日期：1995-06-01 00:00:00

abstract：:DNA gyrase is a type IIA topoisomerase found in bacteria but not in humans. The enzyme is required for bacterial DNA replication and transcription, and is an important antibacterial target that is sensitive to the widely-used fluoroquinolone drugs. Due to the emergence of fluoroquinolone resistance, the discovery of n...

journal_title：Protein expression and purification

authors： Aung HL,Samaranayaka CU,Enright R,Beggs KT,Monk BC

更新日期：2015-03-01 00:00:00

abstract：:NAD(+)-dependent malic enzyme (NAD-ME) gene from Escherichia coli K12 was inserted into an expression vector pET24b(+) and transformed into E. coli BL21 (DE3). Recombinant NAD-ME was expressed upon IPTG induction, purified with affinity chromatography, and biochemically characterized. The results showed that recombina...

journal_title：Protein expression and purification

authors： Wang J,Tan H,Zhao ZK

更新日期：2007-05-01 00:00:00

abstract：:The catalase gene of Psychrobacter sp. T-3 was cloned, and the gene product (PktA) was overexpressed in Escherichia coli. The specific activity of the purified PktA was slightly lower than that of the native purified enzyme obtained from Psychrobacter sp. T-3. Spectrophotometric measurements of the purified enzymes su...

journal_title：Protein expression and purification

authors： Kimoto H,Matsuyama H,Yumoto I,Yoshimune K

更新日期：2008-06-01 00:00:00

abstract：:While well established in bacterial hosts, the effect of coding sequence variation on protein expression in mammalian systems is poorly characterized outside of viral proteins or proteins from distant phylogenetic families. The potential impact is substantial given the extensive use of mammalian expression systems in ...

journal_title：Protein expression and purification

authors： Carton JM,Sauerwald T,Hawley-Nelson P,Morse B,Peffer N,Beck H,Lu J,Cotty A,Amegadzie B,Sweet R

更新日期：2007-10-01 00:00:00

abstract：:Peptide deformylases (PDFs) catalyze the removal of the formyl group from the N-terminal methionine residue in nascent polypeptide chains in prokaryotes. Its deformylation activity makes PDF an attractive candidate for the biocatalytic deprotection of formylated peptides that are used in chemoenzymatic peptide synthes...

journal_title：Protein expression and purification

authors： Di Toma C,Sonke T,Quaedflieg PJ,Volker Wagner AF,Janssen DB

更新日期：2013-05-01 00:00:00

abstract：:Recombinant OSU VP4 protein, and outer capsid antigen of porcine rotavirus, was purified to a high level from the spent broth of baculovirus-infected Spodoptera frugiperda insect cells. Initial clarification of the broth with a 0-60% ammonium sulfate cut retained 93% of the total VP4. Q-sepharose ion exchange chromato...

journal_title：Protein expression and purification

authors： Juarbe-Osorio LG,Gorziglia M,Betenbaugh MJ

更新日期：1993-06-01 00:00:00

abstract：:The ELR-CXC chemokines are important to neutrophil inflammation in many acute and chronic diseases. Among them, CXCL8 (interleukin-8, IL-8), binds to both the CXCR1 and CXCR2 receptors with high affinity and the expression levels of CXCL8 are elevated in many inflammatory diseases. Recently, an analogue of human CXCL8...

journal_title：Protein expression and purification

authors： Cheng HT,Huang KC,Yu HY,Gao KJ,Zhao X,Li F,Town J,Gordon JR,Cheng JW

更新日期：2008-09-01 00:00:00

abstract：:Characterizing protein complexes and identifying their subunits promote our understanding of the machinery involved in many in vivo processes. Proteomic studies can identify a protein's binding partners, and this can provide insight into how protein complexes function and how they are regulated. In addition, the compo...

journal_title：Protein expression and purification

authors： Banks CA,Kong SE,Washburn MP

更新日期：2012-12-01 00:00:00

abstract：:Glucose-6-phosphate dehydrogenase was purified from human placenta using DEAE-Sepharose fast flow, 2',5'-ADP Sepharose 4B column chromatography, and chromatofocusing on PBE 94 with PB 74. The enzyme was purified with 62% yield and had a specific activity of 87 units per milligram protein. The pH optimum was determined...

journal_title：Protein expression and purification

authors： Aksoy Y,Ogüs IH,Oauzer N

更新日期：2001-03-01 00:00:00

abstract：:The transcription factor IIIC (TFIIIC) is a multisubunit DNA-binding factor required for promoter recognition and TFIIIB assembly on tRNA genes transcribed by RNA polymerase III. Yeast TFIIIC consists of six subunits, organized in the two globular subcomplexes tauA and tauB, which recognize two internal tDNA promoter ...

journal_title：Protein expression and purification

authors： Mylona A,Acker J,Fernández-Tornero C,Sentenac A,Müller CW

更新日期：2006-02-01 00:00:00

abstract：:We present a straightforward, versatile method for expressing and purifying β-amyloid (Aβ40) and transmembrane peptides derived from β-amyloid precursor protein (Aβ55). In principle, these methods should be applicable to other types of strongly aggregating peptides. We start with a DNA plasmid encoding a HexaHis tag w...

journal_title：Protein expression and purification

authors： Zerweck J,Venkata BS,Pittman JM,Srivastava AK,Moore PC,Sachleben JR,Thinakaran G,Meredith SC

更新日期：2019-10-01 00:00:00

abstract：:Taxadiene synthase catalyzes the conversion of the universal precursor of diterpenoids, geranylgeranyl diphosphate, to taxadiene, a key intermediate in Taxol (paclitaxel) biosynthesis. The gene encoding taxadiene synthase was cloned recently. Here we report a method for the heterologous overexpression of cDNA encoding...

journal_title：Protein expression and purification

authors： Huang KX,Huang QL,Wildung MR,Croteau R,Scott AI

更新日期：1998-06-01 00:00:00

abstract：:Chemokine receptors are a specific class of G-protein-coupled receptors (GPCRs) that control cell migration associated with routine immune surveillance, inflammation and development. In addition to their roles in normal physiology, these receptors and their ligands are involved in a large number of inflammatory diseas...

journal_title：Protein expression and purification

authors： Allen SJ,Ribeiro S,Horuk R,Handel TM

更新日期：2009-07-01 00:00:00

abstract：:An alpha-amylase has been purified from the thermophilic fungus Scytalidium thermophilum. A ninefold purification was achieved in a single step using fluidized bed chromatography wherein alginate was used as the affinity matrix. There are at least two isoenzymes as shown by concanavalin A (Con A)-agarose column chroma...

journal_title：Protein expression and purification

authors： Roy I,Sastry MS,Johri BN,Gupta MN

更新日期：2000-11-01 00:00:00

abstract：:Serine hydroxymethyltransferase (SHMT) plays a key role in cell physiology as it participates in the different interconversion pathway of folate coenzymes, provides almost exclusively folate one carbon fragments for the biosynthesis of a variety of end products. For the first time, Mycobacterium leprae glyA gene, enco...

journal_title：Protein expression and purification

authors： Sharma S,Bhakuni V

更新日期：2007-09-01 00:00:00

abstract：:In this work we communicate the heterologous expression of a laccase from Coriolopsis gallica in Pichia pastoris. This enzyme has been reported to efficiently degrade a variety of pollutants such as industrial dyes. The expression strategy included using a previously reported modified α-factor preproleader for enhance...

journal_title：Protein expression and purification

authors： Avelar M,Olvera C,Aceves-Zamudio D,Folch JL,Ayala M

更新日期：2017-08-01 00:00:00

abstract：:We have cloned ansB (YPTB1411) gene from Yersinia pseudotuberculosis Q66CJ2 and constructed stable inducible expression system that overproduce L-asparaginase from Y. pseudotuberculosis (YpA) in Escherichiacoli BL21 (DE3) cells. For purification of YpA we used Q-Sepharose and DEAE-Toyopearl column chromatography. We e...

journal_title：Protein expression and purification

authors： Pokrovskaya MV,Aleksandrova SS,Pokrovsky VS,Omeljanjuk NM,Borisova AA,Anisimova NY,Sokolov NN

更新日期：2012-03-01 00:00:00

abstract：:Bacteriocin, which is produced by lactic acid bacteria (LAB), has the potential to act as natural preservatives in the food industry. To develop strategies to overproduce such peptides, plantaricin NC8, a class IIb LAB bacteriocin that consists of two peptides, PLNC8α and PLNC8β, was successfully heterologously expres...

journal_title：Protein expression and purification

authors： Jiang H,Li P,Gu Q

更新日期：2016-11-01 00:00:00

abstract：:The gene coding for phosphoglucomutase (PGM) from Oryctolagus cuniculus (rabbit) has been expressed in Escherichia coli under a T7 expression system with a His-tag. About half of the expressed PGM protein was present in inclusion bodies, but this protein was inactive when solubilized. The protein in the soluble cell f...

journal_title：Protein expression and purification

authors： Chae YK,Markley JL

更新日期：2000-10-01 00:00:00

abstract：:Failure to express soluble proteins in bacteria is mainly attributed to the properties of the target protein itself, as well as the choice of the vector, the purification tag and the linker between the tag and protein, and codon usage. The expression of proteins with fusion tags to facilitate subsequent purification s...

journal_title：Protein expression and purification

authors： Kurz M,Cowieson NP,Robin G,Hume DA,Martin JL,Kobe B,Listwan P

更新日期：2006-11-01 00:00:00

abstract：:We examined the feasibility of high-level production of recombinant human prolactin, a multifunctional protein hormone, in insect cells using a baculovirus expression system. The human prolactin cDNA with and without the secretory signal sequence was cloned into pFastBac1 baculovirus vector under the control of polyhe...

journal_title：Protein expression and purification

authors： Das T,Johns PW,Goffin V,Kelly P,Kelder B,Kopchick J,Buxton K,Mukerji P

更新日期：2000-11-01 00:00:00

abstract：:Refolding of proteins from inclusion bodies is affected by several factors, including solubilization of inclusion bodies by denaturants, removal of the denaturant, and assistance of refolding by small molecule additives. We will review key parameters associated with (1) conformation of the protein solubilized from inc...

journal_title：Protein expression and purification

authors： Tsumoto K,Ejima D,Kumagai I,Arakawa T

更新日期：2003-03-01 00:00:00

abstract：:The sequence and structure of the target protein exert a marked effect on its soluble expression in Escherichia coli. The effects of the mutation of an amylase isolated from Bacillus licheniformis (BLA) on its soluble expression in E. coli were investigated. A random mutation library of BLA was constructed to screen f...

journal_title：Protein expression and purification

authors： Wang P,Qin W,Xu J,Yan Y,Tian J,Wu N,Yao B

更新日期：2016-04-01 00:00:00

abstract：:Receptor activator of NF-kappaB ligand (RANKL) is a type II transmembrane protein found on osteoblasts which functions as a major determinant of osteoclast differentiation and activation. RANKL mediates bone homeostasis through binding to the cognate ligand on osteoclasts, RANK, and a soluble decoy receptor, osteoprot...

journal_title：Protein expression and purification

authors： Willard D,Chen WJ,Barrett G,Blackburn K,Bynum J,Consler T,Hoffman C,Horne E,Iannone MA,Kadwell S,Parham J,Ellis B

更新日期：2000-10-01 00:00:00

abstract：:There is no high-resolution structure for the membrane domain of the human erythrocyte anion exchanger, AE1 (Band 3). In this report, we have developed an expression and purification strategy for AE1 to be used in crystallization trials. Saccharomyces cerevisiae strain BJ5457 was transformed with an expression vector ...

journal_title：Protein expression and purification

authors： Bonar P,Casey JR

更新日期：2010-11-01 00:00:00

abstract：:Plants possess very large numbers of biosynthetic cytochrome P450 enzymes. In spite of the importance of these enzymes for the synthesis of bioactive plant secondary metabolites, only two plant P450 structures has been obtained to date. Isoflavone synthase (IFS) is a membrane-associated cytochrome P450 enzyme catalyzi...

journal_title：Protein expression and purification

authors： Chang Z,Wang X,Wei R,Liu Z,Shan H,Fan G,Hu H

更新日期：2010-12-04 00:00:00

abstract：:To study the subunit structure and the active site of human immunodeficiency virus reverse transcriptase (RT), the enzyme was expressed in E. coli and purified to homogeneity in large quantities. The recombinant enzyme consists of two major polypeptides of 66,000 and 53,000 Da in equimolar amounts and a minor species ...

journal_title：Protein expression and purification

authors： Fischer M,Lifshitz R,Katz T,Liefer I,Ben-Artzi H,Gorecki M,Panet A,Zeelon E

更新日期：1992-08-01 00:00:00

abstract：:The Ig-binding properties of protein L from Peptostreptococcus magnus and protein G from Streptococcus have been successfully combined through the construction of a novel hybrid protein, consisting of a single Ig-binding domain from each protein. The biophysical and biochemical properties of this construct have been c...

journal_title：Protein expression and purification

authors： Harrison SL,Housden NG,Bottomley SP,Cossins AJ,Gore MG

更新日期：2008-03-01 00:00:00

abstract：:Novel GSH-AP (phenoxyl agarose coated gigaporous polystyrene, Agap-co-PSt) microspheres were successfully prepared by introducing GSH ligand into hydrophilic AP microspheres pre-activated with 1,4-butanediol diglycidyl ether. The gigaporous structure and chromatographic properties of GSH-AP medium were evaluated and c...

journal_title：Protein expression and purification

authors： Huang Y,Zhang R,Li J,Li Q,Su Z,Ma G

更新日期：2014-03-01 00:00:00