Abstract:
:The transcription factor IIIC (TFIIIC) is a multisubunit DNA-binding factor required for promoter recognition and TFIIIB assembly on tRNA genes transcribed by RNA polymerase III. Yeast TFIIIC consists of six subunits, organized in the two globular subcomplexes tauA and tauB, which recognize two internal tDNA promoter elements, the A and the B block, respectively. As a first step toward a detailed structural analysis of TFIIIC, we report here the expression, proteolytic analysis, reconstitution, and crystallization of the complex between yeast TFIIIC subunits tau91 and tau60. Proteolysis provided an insight into the domain structure of tau60 and tau91. Both the proteins form a stable complex that does not require an N-terminal, protease-sensitive extension of tau91. Crystals diffracting beyond 3.2 A were obtained from a complex formed by full-length tau60 and the N-terminally truncated form of tau91 lacking this extension.
journal_name
Protein Expr Purifjournal_title
Protein expression and purificationauthors
Mylona A,Acker J,Fernández-Tornero C,Sentenac A,Müller CWdoi
10.1016/j.pep.2005.06.013keywords:
subject
Has Abstractpub_date
2006-02-01 00:00:00pages
255-61issue
2eissn
1046-5928issn
1096-0279pii
S1046-5928(05)00226-3journal_volume
45pub_type
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