Abstract：
:In this work we communicate the heterologous expression of a laccase from Coriolopsis gallica in Pichia pastoris. This enzyme has been reported to efficiently degrade a variety of pollutants such as industrial dyes. The expression strategy included using a previously reported modified α-factor preproleader for enhanced secretion and pAOX1, a methanol-responsive promoter. Methanol concentration, copper salts concentration and temperature were varied in order to enhance laccase expression in this heterologous system. A volumetric activity of 250 U/L was achieved after 12-day culture in Fernbach flasks. The protein was recovered from the supernatant and purified, obtaining a preparation with 90% electrophoretic purity. The catalytic constants of the recombinant enzyme are almost identical to the fungal enzyme, thus rendering this system a useful tool for protein engineering of laccase from C. gallica.
journal_name
Protein Expr Purifjournal_title
Protein expression and purificationauthors
Avelar M,Olvera C,Aceves-Zamudio D,Folch JL,Ayala Mdoi
10.1016/j.pep.2017.06.001subject
Has Abstractpub_date
2017-08-01 00:00:00pages
14-19eissn
1046-5928issn
1096-0279pii
S1046-5928(17)30095-5journal_volume
136pub_type
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