Yeast chromatin reconstitution system using purified yeast core histones and yeast nucleosome assembly protein-1.

abstract：:Antibodies specific to β-Glucocerebrosidase were selected from phage displayed naïve scFv libraries. Biopannings were performed against recombinant human protein β-Glucocerebrosidase immobilized on polystyrene surface, specific phages were eluted with 50% ethylene glycol in citrate buffer (pH 6.0). Several specific bi...

journal_title：Protein expression and purification

authors： Anisimov RL,Ershova OA,Ershov AV,Filatova MA,Katorkin SA,Simonov VM

更新日期：2020-06-01 00:00:00

abstract：:Endostatin, a 20kDa C-terminal fragment of collagen XVIII, is a potent anti-angiogenic protein and inhibitor of tumor growth. Recombinant endostatin was prepared from Escherichia coli deposited as insoluble, inactive inclusion bodies. In the present study, we produced soluble and biologically active recombinant human ...

journal_title：Protein expression and purification

authors： Xu HM,Zhang GY,Ji XD,Cao L,Shu L,Hua ZC

更新日期：2005-06-01 00:00:00

abstract：:The hepatitis E virus (HEV) capsid antigen has been proposed as a candidate subunit vaccine for the prevention of hepatitis E. The full-length HEV ORF2 protein product is predicted to contain 660 amino acids and to weigh 72,000 daltons. Expression of the HEV ORF2 capsid gene from recombinant baculoviruses in insect ce...

journal_title：Protein expression and purification

authors： Robinson RA,Burgess WH,Emerson SU,Leibowitz RS,Sosnovtseva SA,Tsarev S,Purcell RH

更新日期：1998-02-01 00:00:00

abstract：:Alphavirus nonstructural protein nsP1 possesses distinct methyltransferase (MTase) and guanylyltransferase (GTase) activities involved in the capping of viral RNAs. In alphaviruses, the methylation of GTP occurs before RNA transguanylation and nsP1 forms a covalent complex with m(7)GMP unlike the host mRNA guanylyltra...

journal_title：Protein expression and purification

authors： Tomar S,Narwal M,Harms E,Smith JL,Kuhn RJ

更新日期：2011-10-01 00:00:00

abstract：:Transmembrane and coiled-coil domains 1 (TMCO1) has a highly conserved amino acid sequence among species, indicating a critical role of TMCO1 in cell physiology. The deficiency of TMCO1 in humans is associated with cerebrofaciothoracic dysplasia (CFTD), glaucoma, osteogenesis and the occurrence of cancer. TMCO1 was re...

journal_title：Protein expression and purification

authors： Zhang N,Tang M,Wen M,Cao Y,OuYang B

更新日期：2021-03-01 00:00:00

abstract：:The methylotrophic yeasts Pichia pastoris and Pichia angusta (Hansenula polymorpha) were used for the comparative heterologous production of two model mammalian proteins of pharmaceutical interest, the NK1-fragment (22 kDa) of human hepatocyte growth factor and the extracellular domain (28 kDa) of mouse tissue factor ...

journal_title：Protein expression and purification

authors： Mack M,Wannemacher M,Hobl B,Pietschmann P,Hock B

更新日期：2009-08-01 00:00:00

abstract：:We have cloned, from total human liver RNA, the cDNA encoding apolipoprotein E3 (apoE3). Site-directed mutagenesis was used to obtain the cDNA encoding the apoE4 isoform, a major variant of this apolipoprotein in man. These two cDNAs were subcloned into the procaryotic expression vector pAHRS. A polyhistidine tag was ...

journal_title：Protein expression and purification

authors： Pillot T,Barbier A,Visvikis A,Lozac'h K,Rosseneu M,Vandekerckhove J,Siest G

更新日期：1996-06-01 00:00:00

abstract：:The human iron-binding protein lactoferrin (hLf) has been implicated in a number of important physiological pathways, including those regulating immune function and tumor growth. In an effort to develop an efficient system for production of recombinant hLf (rhLf) that is structurally and functionally equivalent to the...

journal_title：Protein expression and purification

authors： Tutykhina IL,Bezborodova OA,Shmarov MM,Logunov DY,Neugodova GL,Nemtsova ER,Naroditsky BS,Yakubovskaya RI,Gintsburg AL

更新日期：2009-05-01 00:00:00

abstract：:Structural studies of human peptide hormone somatostatin 14 (SS14) require high amounts of isotopically labelled SS14 to be produced. Here we report a method for effective production of isotopically labelled SS14. SS14 was expressed as a fusion protein with thioredoxin in Escherichia coli. Co-expression of a longer po...

journal_title：Protein expression and purification

authors： Nespovitaya N,Barylyuk K,Eichmann C,Zenobi R,Riek R

更新日期：2014-07-01 00:00:00

abstract：:Trichoderma reesei tyrosinase TYR2 has been demonstrated to be able to oxidize various phenolic compounds and also peptide and protein bound tyrosine, and thus is of great interest for different biotechnological applications. In order to understand the reaction mechanism of the enzyme it would be essential to solve it...

journal_title：Protein expression and purification

authors： Westerholm-Parvinen A,Selinheimo E,Boer H,Kalkkinen N,Mattinen M,Saloheimo M

更新日期：2007-09-01 00:00:00

abstract：:The covalent addition of 4-amino-4-deoxy-L-arabinose (L-Ara4N) groups to lipid A, which resides in the outer membranes of bacteria such as Salmonella typhimurium and Escherichia coli, is the final step in the polymyxin-resistance pathway in these organisms. This modification is catalyzed by the inner membrane protein ...

journal_title：Protein expression and purification

authors： Bretscher LE,Morrell MT,Funk AL,Klug CS

更新日期：2006-03-01 00:00:00

abstract：:Production of antibody fragments in heterologous hosts such as Escherichiacoli provides a unique and cost-effective method to develop engineered vectors for tumor targeting. A single-chain Fragment variable (scFv) of the murine monoclonal antibody MAb-B43.13 targeting CA125 in epithelial ovarian cancer was previously ...

journal_title：Protein expression and purification

authors： Sharma SK,Suresh MR,Wuest FR

更新日期：2014-10-01 00:00:00

abstract：:Pectate lyase (EC 4.2.2.2) gene from Bacillus subtilis RCK was cloned and expressed in Escherichia coli to maximize its production. In addition to soluble fraction, bioactive pectate lyase was also obtained from inclusion body aggregates by urea solubilization and refolding under in vitro conditions. Enzyme with speci...

journal_title：Protein expression and purification

authors： Kumar S,Jain KK,Singh A,Panda AK,Kuhad RC

更新日期：2015-06-01 00:00:00

abstract：:Aquaporins are integral membrane channel proteins found in all kingdoms of life. The Escherichia coli aquaporin Z (AqpZ) has been shown to solely conduct water at high permeability. Functional AqpZ is generally purified from the membrane fraction. However, the quantity of the purified protein is limited. In this study...

journal_title：Protein expression and purification

authors： Wang L,Zhou H,Li Z,Lim TK,Lim XS,Lin Q

更新日期：2015-11-01 00:00:00

abstract：:High affinity interaction between S-protein and S-peptide fragments of bovine pancreatic RNase A has been recently used for construction of molecular vehicles for targeted drug delivery. The vehicle is assembled as a complex of drug carrier conjugated S-protein with S-peptide-tagged targeting protein. To avoid random ...

journal_title：Protein expression and purification

authors： Backer MV,Gaynutdinov TI,Aloise R,Przekop K,Backer JM

更新日期：2002-12-01 00:00:00

abstract：:The UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase from Escherichia coli, an enzyme involved in the biosynthesis of the bacterial peptidoglycan monomer unit, was overproduced and purified to homogeneity on a large scale, yielding 4 mg of protein per liter of bacterial culture. Crystals of the complex with the subst...

journal_title：Protein expression and purification

authors： Auger G,Martin L,Bertrand J,Ferrari P,Fanchon E,Vaganay S,Pétillot Y,van Heijenoort J,Blanot D,Dideberg O

更新日期：1998-06-01 00:00:00

abstract：:A method was developed for the affinity purification of human complement properdin. The preparation is part of an integrated scheme in which over 20 human plasma proteins can be recovered in a highly purified form. The yield of properdin was 5.9 mg from 3 liters of plasma, amounting to a 28% recovery. The crucial step...

journal_title：Protein expression and purification

authors： DiScipio RG

更新日期：1994-04-01 00:00:00

abstract：:Enzymatic hydrolysis of the N-iminylamide was investigated in this study. An enzyme possessing N-iminylamidase activity from pig liver was purified to electrophoretic homogeneity. This enzyme was also active, however, with imides and appears to be identical to pig liver imidase. The identification was confirmed by cop...

journal_title：Protein expression and purification

authors： Huang CY,Yang YS

更新日期：2005-03-01 00:00:00

abstract：:We have successfully expressed and purified active human glycogen synthase-1 (hGYS1). Successful production of the recombinant hGYS1 protein was achieved by co-expression of hGYS1 and rabbit glycogenin (rGYG1) using the MultiBac baculovirus expression system (BEVS). Functional measurements of activity ratios of hGYS1 ...

journal_title：Protein expression and purification

authors： Khanna M,Imasaki T,Chikwana VM,Perez-Miller S,Hunter GO,Mosley A,Takagi Y,Hurley TD

更新日期：2013-08-01 00:00:00

abstract：:Cysteine-rich intestinal protein (CRIP) is a small, 8.5-kDa protein with one double zinc-finger motif called a LIM domain. It is very abundant in intestine and some immune cells in rodents, and expression is influenced by development and the immune response. We have cloned a human CRIP cDNA from human small intestine ...

journal_title：Protein expression and purification

authors： Khoo C,Blanchard RK,Sullivan VK,Cousins RJ

更新日期：1997-04-01 00:00:00

abstract：:Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), which is capable of selectively inducing apoptosis of cancer cells, is a potential targeted drug for cancer therapy. The TRAIL protein induces apoptosis only in trimeric form. However, the recombinant soluble TRAIL (sTRAIL) trimer has low stability and a...

journal_title：Protein expression and purification

authors： Wang B,Wang Z,Yan J,Wang L,Wang Z,Wu J,Zhang H,Wu H,Kong W,Yu B,Yu X

更新日期：2015-11-01 00:00:00

abstract：:Escherichia coli glutamic acid decarboxylase is a pyridoxal phosphate-dependent enzyme that catalyzes the alpha-decarboxylation of glutamate to yield 4-amino-butyrate and CO2. The E. coli chromosome contains two genes encoding for this enzyme, gadA and gadB, which map at distinct loci. Their protein products differ in...

journal_title：Protein expression and purification

authors： De Biase D,Tramonti A,John RA,Bossa F

更新日期：1996-12-01 00:00:00

abstract：:Pullulanases are well-known starch-debranching enzymes that are widely used for hydrolysis of a-1,6-glycosidic linkages in starch, pullulan, amylopectin, and other oligosaccharides. Escherichia coli is a popular heterologous expression host for generating target enzymes. However, cells have to be disrupted to obtain t...

journal_title：Protein expression and purification

authors： Wang X,Chen Y,Nie Y,Xu Y

更新日期：2019-03-01 00:00:00

abstract：:Amidase signature (AS) family amidases are known to exhibit broad substrate specificity. According to the available genome sequence data, a novel AS family amidase, Pl-Ami, was identified and cloned from the genome of Parvibaculum lavamentivorans ZJB14001. The recombinant amidase was overexpressed in Escherichia coli ...

journal_title：Protein expression and purification

authors： Wu ZM,Zheng RC,Zheng YG

更新日期：2017-01-01 00:00:00

abstract：:The presence of antibiotic resistance genes in genetically modified bacteria raises a regulatory concern in the production of therapeutic proteins and additionally reduces the number of plasmids available for propagation in a cell. Cre recombinase from bacteriophage P1, involved in Cre/loxP mechanism is one of the wid...

journal_title：Protein expression and purification

authors： D S,Shyam Mohan AH,Rao SN

更新日期：2020-03-01 00:00:00

abstract：:For affinity-chromatography-based purification of proteins that are prone to abnormal termination of translation or that may not be modified at their N-termini, affinity tags are needed which can be fused to the C-terminus. In this publication we describe that maltose binding protein (MBP) fused to the C-terminus of t...

journal_title：Protein expression and purification

authors： Hennig L,Schäfer E

更新日期：1998-12-01 00:00:00

abstract：:α-N-Acetylgalactosaminidase (α-GalNAc-ase; EC.3.2.1.49) is an exoglycosidase specific for the hydrolysis of terminal α-linked N-acetylgalactosamine in various sugar chains. The cDNA corresponding to the α-GalNAc-ase gene was cloned from Aspergillus niger, sequenced, and expressed in the yeast Saccharomyces cerevisiae....

journal_title：Protein expression and purification

authors： Mrázek H,Benada O,Man P,Vaněk O,Křen V,Bezouška K,Weignerová L

更新日期：2012-01-01 00:00:00

abstract：:Using a codon-optimized gene fragment, we report remarkable yields for extracellular domain of human NK cell receptor (NKp30ex) when produced on M9 minimal medium, even with low (2g/L) glucose concentration. The yields were identical using media containing (15)NH(4)Cl or (15)NH(4)Cl in combination with all-(13)C-d-glu...

journal_title：Protein expression and purification

authors： Grave L,Tůmová L,Mrázek H,Kavan D,Chmelík J,Vaněk O,Novák P,Bezouška K

更新日期：2012-12-01 00:00:00

abstract：:A stable mammalian cell line expressing highly active bovine interferon-gamma (BoIFN-γ) was generated using Flp recombinase-mediated integration. This recombinant 293 cell line (B1) efficiently secreted FLAG-tagged BoIFN-γ protein into the culture supernatant, as determined by ELISA and Western blot. The recombinant B...

journal_title：Protein expression and purification

authors： Xu Z,Shan F,Shan F,Meng C,Zhou X,Zhang X,Chen X,Jiao X

更新日期：2014-07-01 00:00:00

abstract：:Expressed protein libraries are becoming a critical tool for new target discovery in the pharmaceutical industry. In order to get the most meaningful and comprehensive results from protein library screens, it is essential to have library proteins in their native conformation with proper post-translation modifications....

journal_title：Protein expression and purification

authors： Panavas T,Lu J,Liu X,Winkis AM,Powers G,Naso MF,Amegadzie B

更新日期：2011-09-01 00:00:00