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Expression and purification of functional human glycogen synthase-1 (hGYS1) in insect cells.

Abstract：

:We have successfully expressed and purified active human glycogen synthase-1 (hGYS1). Successful production of the recombinant hGYS1 protein was achieved by co-expression of hGYS1 and rabbit glycogenin (rGYG1) using the MultiBac baculovirus expression system (BEVS). Functional measurements of activity ratios of hGYS1 in the absence and presence of glucose-6-phosphate and treatment with phosphatase indicate that the expressed protein is heavily phosphorylated. We used mass spectrometry to further characterize the sites of phosphorylation, which include most of the known regulatory phosphorylation sites, as well as several sites unique to the insect cell over-expression. Obtaining large quantities of functional hGYS1 will be invaluable for future structural studies as well as detailed studies on the effects on specific sites of phosphorylation.

journal_name

Protein Expr Purif

journal_title

Protein expression and purification

authors

Khanna M,Imasaki T,Chikwana VM,Perez-Miller S,Hunter GO,Mosley A,Takagi Y,Hurley TD

doi

10.1016/j.pep.2013.05.007

subject

Has Abstract

pub_date

2013-08-01 00:00:00

pages

78-83

issue

2

eissn

1046-5928

issn

1096-0279

pii

S1046-5928(13)00093-4

journal_volume

90

pub_type

杂志文章

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