Characterization of recombinant pectate lyase refolded from inclusion bodies generated in E. coli BL21(DE3).

abstract：:Vascular leak syndrome (VLS) is the major dose-limiting toxicity of immunotoxin therapy. In our previous study, a modified PE38KDEL, denoted PE38KDELKQK, was engineered to eliminate VLS. The PE38KDELKQK-based immunotoxin has been proved to retain potent anti-tumor activity but with a remarkable attenuation in VLS. In ...

journal_title：Protein expression and purification

authors： Wang H,Dai J,Li B,Fan K,Peng L,Zhang D,Cao Z,Qian W,Wang H,Zhao J,Guo Y

更新日期：2008-03-01 00:00:00

abstract：:Baculovirus mediated gene transduction of mammalian cells (BacMam) is an emerging technique for rapid recombinant protein expression in mammalian cells. We constructed two baculovirus transfer vectors that incorporate several mammalian transcriptional regulatory elements necessary for high-level protein expression in ...

journal_title：Protein expression and purification

authors： Dukkipati A,Park HH,Waghray D,Fischer S,Garcia KC

更新日期：2008-12-01 00:00:00

abstract：:Mitochondrial 2,4-dienoyl-CoA reductase is a key enzyme for the beta-oxidation of unsaturated fatty acids. The cDNA of the full-length human mitochondrial 2,4-dienoyl-CoA reductase was previously cloned as pUC18::DECR. PCR methodologies were used to subclone the genes encoding various truncated human mitochondrial 2,4...

journal_title：Protein expression and purification

authors： Chu X,Yu W,Chen G,Li D

更新日期：2003-10-01 00:00:00

abstract：:Dengue virus causes serious diseases affecting people in tropical and sub-tropical regions. The nonstructural (NS) protein 2B is an integral membrane protein and important for the regulation of viral protease NS3, which is significant for virus replication. The NS2B-NS3 complex is an important drug target for treating...

journal_title：Protein expression and purification

authors： Huang Q,Chen AS,Li Q,Kang C

更新日期：2011-12-01 00:00:00

abstract：:Resistance to Inhibitors of Cholinesterase-8 (Ric-8) proteins are molecular chaperones that fold heterotrimeric G protein α subunits shortly after biosynthesis. Ric-8 proteins also act as test tube guanine nucleotide exchange factors (GEF) that promote Gα subunit GDP for GTP exchange. The GEF and chaperoning activitie...

journal_title：Protein expression and purification

authors： Yu W,Yu M,Papasergi-Scott MM,Tall GG

更新日期：2019-02-01 00:00:00

abstract：:The human iron-binding protein lactoferrin (hLf) has been implicated in a number of important physiological pathways, including those regulating immune function and tumor growth. In an effort to develop an efficient system for production of recombinant hLf (rhLf) that is structurally and functionally equivalent to the...

journal_title：Protein expression and purification

authors： Tutykhina IL,Bezborodova OA,Shmarov MM,Logunov DY,Neugodova GL,Nemtsova ER,Naroditsky BS,Yakubovskaya RI,Gintsburg AL

更新日期：2009-05-01 00:00:00

abstract：:An unknown d-tagatose 3-epimerase (DTE) containing a IoIE domain was identified and cloned from Escherichia coli. This gene was subcloned into the prokaryotic expression vector pET-15b, and induced by IPTG in E. coli BL21 expression system. Through His-select gel column purification and fast-protein liquid chromatogra...

journal_title：Protein expression and purification

authors： He X,Zhou X,Yang Z,Xu L,Yu Y,Jia L,Li G

更新日期：2015-10-01 00:00:00

abstract：:The whole encoding sequence for Yersinia pestis LcrV antigen was cloned into pET-32a(+) and expressed in Escherichia coli BL21 (DE3). The LcrV was high level expressed in the E. coli cytoplasm in a completely soluble form. Recombinant LcrV could be purified from the supernatant of the bacteria lysate after chromatogra...

journal_title：Protein expression and purification

authors： li J,Li B,Li G,Ren J,Zhang J,Xu C,Yang X,Liu S,Fu L,Chen W

更新日期：2008-02-01 00:00:00

abstract：:Plantaricin JK (PlnJK) is a Class IIb LAB bacteriocin that includes two peptides; i.e., PlnJ and PlnK, which can synergistically halt many types of gram-positive bacteria, including food spoilage organisms. Purification of these peptides from natural lactic acid bacteria is difficult therefore, their application remai...

journal_title：Protein expression and purification

authors： Xu Y,Yang L,Li P,Gu Q

更新日期：2019-07-01 00:00:00

abstract：:17β-hydroxysteroid dehydrogenase type 1 (17β-HSD1) mainly catalyzes the reduction of estrone into estradiol. The enzymatic conversion is a critical step in estradiol accumulation in breast tissue, which is a valuable prognosis index of breast cancer disease. However, the source of 17β-HSD1 for inhibitor design is limi...

journal_title：Protein expression and purification

authors： Chen J,Feng W,Zhao Y

更新日期：2017-09-01 00:00:00

abstract：:An antifungal protein with a chitinase-like N-terminal sequence, designated delandin, was isolated from the rice bean. The protein exhibited a molecular weight of 28 kDa and was adsorbed on both blue Affi-Gel and SP-Toyopearl. It exerted antifungal action toward Mycosphaerella arachidicola, Botrytis cinerea, Fu- sariu...

journal_title：Protein expression and purification

authors： Ye XY,Ng TB

更新日期：2002-04-01 00:00:00

abstract：:We present a straightforward, versatile method for expressing and purifying β-amyloid (Aβ40) and transmembrane peptides derived from β-amyloid precursor protein (Aβ55). In principle, these methods should be applicable to other types of strongly aggregating peptides. We start with a DNA plasmid encoding a HexaHis tag w...

journal_title：Protein expression and purification

authors： Zerweck J,Venkata BS,Pittman JM,Srivastava AK,Moore PC,Sachleben JR,Thinakaran G,Meredith SC

更新日期：2019-10-01 00:00:00

abstract：:Structural studies of human peptide hormone somatostatin 14 (SS14) require high amounts of isotopically labelled SS14 to be produced. Here we report a method for effective production of isotopically labelled SS14. SS14 was expressed as a fusion protein with thioredoxin in Escherichia coli. Co-expression of a longer po...

journal_title：Protein expression and purification

authors： Nespovitaya N,Barylyuk K,Eichmann C,Zenobi R,Riek R

更新日期：2014-07-01 00:00:00

abstract：:Bikunin is a proteoglycan exhibiting broad-spectrum inhibitory activity against serine proteases and could potentially suppress tumor cell invasion and metastasis. Here, we have successfully expressed recombinant human bikunin (rh-bikunin) in the methylotrophic yeast Pichia pastoris and established the purification pr...

journal_title：Protein expression and purification

authors： Jian-qiu W,Feng-qin Y,Dou-dou W,Lei B,Nan S,Cong-yan L,Ting Z,Wei-qun Y

更新日期：2008-08-01 00:00:00

abstract：:The expression of recombinant proteins in bacterial hosts may alter the biophysical properties of the protein of interest as a result of differences in post-translational processing from that observed when produced in the native cell. For example, recombinant human interleukin-1beta (IL-1beta) is produced as three iso...

journal_title：Protein expression and purification

authors： Covalt JC Jr,Cao TB,Magdaroag JR,Gross LA,Jennings PA

更新日期：2005-05-01 00:00:00

abstract：:In microorganisms and plants, mevalonate kinase is involved in the biosynthesis of isoprenoid derivatives, one of the largest groups of natural products. We subcloned the gene of mevalonate kinase from Methanococcus jannaschii into a bacterial expression vector pLM1 with six continuous histidine codons attached to the...

journal_title：Protein expression and purification

authors： Chu X,Liu X,Yau M,Leung YC,Li D

更新日期：2003-08-01 00:00:00

abstract：:A hexahistidine tag was fused to the N-terminus of apoaequorin. A suitable vector encoding the fusion protein was constructed and used for transformation of Escherichia coli JM109 cells. Apoaequorin was overexpressed under the control of tac promoter. It was found, however, that most of the protein existed in the form...

journal_title：Protein expression and purification

authors： Glynou K,Ioannou PC,Christopoulos TK

更新日期：2003-02-01 00:00:00

abstract：:Recombinant human interleukin-5 (rhIL-5) was expressed in baculovirus-infected insect cells and purified to homogeneity from the culture medium in a single chromatographic step. Beginning with a cDNA encoding the full-length precursor form of human IL-5, including the authentic secretory leader sequence, recombinant b...

journal_title：Protein expression and purification

authors： Brown PM,Scheid MP,O'Neill GP,Tagari PC,Nicholson DW

更新日期：1995-02-01 00:00:00

abstract：:The XRCC1 DNA repair protein contains two regions of approximately 100 amino acids each that share homology with the BRCT (BRCA1 carboxyl terminus) domain superfamily. These two regions of XRCC1 have been shown to interact independently with DNA ligase III and poly(ADP-ribose)polymerase as part of a mechanism involved...

journal_title：Protein expression and purification

authors： Thornton K,Forstner M,Shen MR,West MG,Rupp B,Thelen MP

更新日期：1999-07-01 00:00:00

abstract：:Na+, K+-ATPase beta2 subunit (NKA1b2) is not only a regulator of Na+, K+-ATPase, but also functions in the interaction between neuron and glia cells as a Ca2+-dependent adhesion molecule. To further study the function of NKA1b2, the anti-NKA1b2 polyclonal antibody was prepared to recognize the outer-membrane carboxyl ...

journal_title：Protein expression and purification

authors： Chen TF,Zhang YL,Xu WL,Li ZQ,Hou B,Wang CL,Fan M,Qian LJ,Zhou RP,Zhang CG

更新日期：2004-09-01 00:00:00

abstract：:We present a new streptavidin-binding peptide for both the purification and the detection of recombinant proteins. The peptide possesses nanomolar-affinity for streptavidin and therefore was termed Nano-tag. The Nano-tag(15) is 15 amino acids long and binds to streptavidin with a dissociation constant of 4 nM and the ...

journal_title：Protein expression and purification

authors： Lamla T,Erdmann VA

更新日期：2004-01-01 00:00:00

abstract：:L1 is a human cell adhesion glycoprotein involved in the development of the central nervous system that comprises six immunoglobulin-like domains (Ig1-Ig6), five fibronectin-type III (FN1-FN5) domains, a single transmembrane region and a cytoplasmic domain. It contains 20 potential N-glycosylation sites and is heavily...

journal_title：Protein expression and purification

authors： Gouveia RM,Morais VA,Peixoto C,Sousa M,Regalla M,Alves PM,Costa J

更新日期：2007-03-01 00:00:00

abstract：:2-Deoxy-d-ribose-5-phosphate aldolase (DERA) catalyzes the aldol reaction between two aldehydes and is thought to be a potential biocatalyst for the production of a variety of stereo-specific materials. A gene encoding DERA from the extreme halophilic archaeon, Haloarcula japonica, was overexpressed in Escherichia col...

journal_title：Protein expression and purification

authors： Ohshida T,Hayashi J,Satomura T,Kawakami R,Ohshima T,Sakuraba H

更新日期：2016-10-01 00:00:00

abstract：:Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit epsilon N-methyltransferase (Protein methylase III, Rubisco LSMT, EC 2.1.1.43) catalyzes methylation of the epsilon-amino group of Lys-14 in the large subunit of Rubisco. In this paper, an affinity purification procedure for pea (Pisum sativum L. cv Laxton'...

journal_title：Protein expression and purification

authors： Wang P,Royer M,Houtz RL

更新日期：1995-08-01 00:00:00

abstract：:This article reviews the development of immobilized-metal affinity chromatography (IMAC) and describes its most important applications. We provide an overview on the use of IMAC in protein fractionation and proteomics, in protein immobilization and detection, and on some special applications such as purification of im...

journal_title：Protein expression and purification

authors： Block H,Maertens B,Spriestersbach A,Brinker N,Kubicek J,Fabis R,Labahn J,Schäfer F

更新日期：2011-09-03 00:00:00

abstract：:The catalase gene of Psychrobacter sp. T-3 was cloned, and the gene product (PktA) was overexpressed in Escherichia coli. The specific activity of the purified PktA was slightly lower than that of the native purified enzyme obtained from Psychrobacter sp. T-3. Spectrophotometric measurements of the purified enzymes su...

journal_title：Protein expression and purification

authors： Kimoto H,Matsuyama H,Yumoto I,Yoshimune K

更新日期：2008-06-01 00:00:00

abstract：:To expand the application of the streptavidin-biotin technology for reversible affinity purification of biotinylated proteins, a novel form of monomeric streptavidin was engineered and produced using Bacillus subtilis as the expression host. By changing as little as two amino acid residues (T90 and D128) to alanine, t...

journal_title：Protein expression and purification

authors： Qureshi MH,Wong SL

更新日期：2002-08-01 00:00:00

abstract：:L-Threonine dehydrogenase was purified 10,000-fold to a specific activity approximately 300 mumol.min-1.mg-1 protein from porcine liver mitochondria. Purification to apparent homogeneity was achieved by sequential chromatography on DEAE Sepharose FF, Affi-Gel Blue, Sephacryl S-200, Matrex Gel Red A, and Matrex Gel Gre...

journal_title：Protein expression and purification

authors： Kao YC,Davis L

更新日期：1994-10-01 00:00:00

abstract：:Our earlier studies have demonstrated that the 35 kDa isoform of Translocated promoter region protein (Tpr) of Rattus norvegicus was able to augment c-jun transcription efficiently. Identification of direct targets that may in part downregulate c-jun transcription might prove to be an ideal target to curtail the proli...

journal_title：Protein expression and purification

authors： Agarwal S,Yadav SK,Dixit A

更新日期：2011-05-01 00:00:00

abstract：:Hsp47 is regarded as a collagen-specific chaperone with several suggested roles in collagen biosynthesis under normal and disease conditions. We describe here a procedure for the expression and purification of Hsp47 in Escherichia coli using the IMPACT expression system (New England Biolabs) where the guest gene is fu...

journal_title：Protein expression and purification

authors： Thomson CA,Ananthanarayanan VS

更新日期：2001-10-01 00:00:00