A hydrophobic interaction chromatography strategy for purification of inactivated foot-and-mouth disease virus.

abstract：:Pokeweed antiviral protein (PAP)-I from the spring leaves of Phytolacca americana is a naturally occurring RNA-depurinating enzyme with broad-spectrum antiviral activity. Interest in PAP is growing due to its use as a potential anti-HIV agent. However, the clinical use of native PAP is limited due to inherent difficul...

journal_title：Protein expression and purification

authors： Rajamohan F,Doumbia SO,Engstrom CR,Pendergras SL,Maher DL,Uckun FM

更新日期：2000-03-01 00:00:00

abstract：:Hsp47 is regarded as a collagen-specific chaperone with several suggested roles in collagen biosynthesis under normal and disease conditions. We describe here a procedure for the expression and purification of Hsp47 in Escherichia coli using the IMPACT expression system (New England Biolabs) where the guest gene is fu...

journal_title：Protein expression and purification

authors： Thomson CA,Ananthanarayanan VS

更新日期：2001-10-01 00:00:00

abstract：:The TCL1 gene, which is located on chromosome 14, plays a major role in human hematopoietic malignancies and encodes a 14-kDa protein whose function has not been determined. This gene is expressed in pre-B cells, in immature thymocytes, and, at low levels, in activated T cells but not in peripheral mature B cells and ...

journal_title：Protein expression and purification

authors： Du Bois GC,Song SP,Kulikovskaya I,Rothstein JL,Germann MW,Croce CM

更新日期：2000-04-01 00:00:00

abstract：:The full-length human acetyl-CoA carboxylase 1 (ACC1) was expressed and purified to homogeneity by two separate groups (Y.G. Gu, M. Weitzberg, R.F. Clark, X. Xu, Q. Li, T. Zhang, T.M. Hansen, G. Liu, Z. Xin, X. Wang, T. McNally, H. Camp, B.A. Beutel, H.I. Sham, Synthesis and structure-activity relationships of N-{3-[2...

journal_title：Protein expression and purification

authors： Kim KW,Yamane H,Zondlo J,Busby J,Wang M

更新日期：2007-05-01 00:00:00

abstract：:The Ig-binding properties of protein L from Peptostreptococcus magnus and protein G from Streptococcus have been successfully combined through the construction of a novel hybrid protein, consisting of a single Ig-binding domain from each protein. The biophysical and biochemical properties of this construct have been c...

journal_title：Protein expression and purification

authors： Harrison SL,Housden NG,Bottomley SP,Cossins AJ,Gore MG

更新日期：2008-03-01 00:00:00

abstract：:Proteomics has become a major focus as researchers attempt to understand the vast amount of genomic information. Protein complexity makes identifying and understanding gene function inherently difficult. The challenge of studying proteins in a global way is driving the development of new technologies for systematic an...

journal_title：Protein expression and purification

authors： Lesley SA

更新日期：2001-07-01 00:00:00

abstract：:This is the first report of an insect esterase efficiently expressed in the methylotrophic yeast Pichia pastoris (so far insect esterases have been produced only in the baculovirus system). Having isolated a Tribolium castaneum carboxylesterase cDNA (TCE), we were initially unable to express it in Escherichia coli or ...

journal_title：Protein expression and purification

authors： Delroisse JM,Dannau M,Gilsoul JJ,El Mejdoub T,Destain J,Portetelle D,Thonart P,Haubruge E,Vandenbol M

更新日期：2005-08-01 00:00:00

abstract：:A method for the simplified, reproducible production of both normal and altered versions of human proinsulin has been developed. A polyhistidine/proinsulin fusion protein was expressed using a prokaryotic expression system and partially purified by affinity chromatography. Disulfide bonds within the polypeptide were f...

journal_title：Protein expression and purification

authors： Mackin RB

更新日期：1999-04-01 00:00:00

abstract：:Dps, found in many eubacterial and archaebacterial species, appears to protect cells from oxidative stress and/or nutrient-limited environment. Dps has been shown to accumulate during the stationary phase, to bind to DNA non-specifically, and to form a crystalline structure that compacts and protects the chromosome. O...

journal_title：Protein expression and purification

authors： Hanna ES,Roque-Barreira MC,Mendes GM,Soares SG,Brocchi M

更新日期：2008-06-01 00:00:00

abstract：:Plasmids pQE-60 and pQE-30 containing 6 x His-tag sequence were used for expression of fragments of HCV structural and non-structural proteins in Escherichia coli (E. coli). The following fragments were used: core (1-98 aa), NS3 (202-482 aa), and tetramer of hypervariable region 1 (HVR1) of E2 protein. The constructed...

journal_title：Protein expression and purification

authors： Mihailova M,Fiedler M,Boos M,Petrovskis I,Sominskaya I,Roggendorf M,Viazov S,Pumpens P

更新日期：2006-11-01 00:00:00

abstract：:Acyl-acyl carrier protein synthase (Aas) is widely used to synthesize thioester adducts of fatty acids between 8 and 18 carbons in length enzymatically to the phosphopantetheine group of acyl carrier protein. The enzyme is an 80.6-kDa inner membrane protein that functions in vivo as a 2-acylglycerophosphoethanolamine ...

journal_title：Protein expression and purification

authors： Shanklin J

更新日期：2000-04-01 00:00:00

abstract：:A novel and rapid procedure for the isolation of catalase from mouse liver, after prior treatment with the peroxisome proliferator perfluorooctanoic acid was developed using immobilized metal ion affinity chromatography involving chelation with zinc ions. The purification developed is simple, rapid (requiring 3 hours ...

journal_title：Protein expression and purification

authors： Yang Q,DePierre JW

更新日期：1998-03-01 00:00:00

abstract：:The promyelocytic leukemia (PML) gene is involved in the 15/17 chromosomal translocation of acute promyelocytic leukemia (APL). It encodes a nuclear phosphoprotein containing an alpha-helical coiled-coil domain with four heptad repeats. The heptad repeats consist of four clusters of hydrophobic amino acids that mediat...

journal_title：Protein expression and purification

authors： Antolini F,Lo Bello M,Sette M

更新日期：2003-05-01 00:00:00

abstract：:We have developed a mammalian expression system suitable for the production of enzymatically biotinylated integral membrane proteins. The key feature of this system is the doxycycline (dox)-regulated co-expression of a secreted variant of Escherichia coli biotin ligase (BirA) and a target protein with a 13-residue bio...

journal_title：Protein expression and purification

authors： Mize GJ,Harris JE,Takayama TK,Kulman JD

更新日期：2008-02-01 00:00:00

abstract：:Soybean root nodules possess a developmentally regulated acid phosphatase (ACP) that exhibits the highest specificity for purine 5'-nucleoside monophosphates. The enzyme is a glycosylated dimer of 28- and 31-kDa subunits, which appear to be products of the same gene but differ in posttranslational modifications. In or...

journal_title：Protein expression and purification

authors： Penheiter AR,Klucas RV,Sarath G

更新日期：1998-10-01 00:00:00

abstract：:Cyclomaltodextrinases are multidomain and often dimeric proteins from the alpha-amylase family (glycoside hydrolase family 13) which frequently have been very difficult to express in active form in Escherichia coli. To express the soluble form of this type of proteins in larger quantities the expression has to be opti...

journal_title：Protein expression and purification

authors： Turner P,Holst O,Karlsson EN

更新日期：2005-01-01 00:00:00

abstract：:Staphylococcal enterotoxins (SEs) are powerful superantigens that stimulate non-specific T-cell proliferation produced by Staphylococcus aureus and draw considerable attention as ideal drugs for cancer therapy. The filtrate of S. aureus culture has been used as ampul named Staphylococcal enterotoxin C injection in cli...

journal_title：Protein expression and purification

authors： Pan YQ,Ding D,Li DX,Chen SQ

更新日期：2007-12-01 00:00:00

abstract：:Recombinant murine MRP14 (mMRP14) was produced in Escherichia coli using the pGEX expression system. The mass of fusion protein, by electrospray ionization-mass spectrometry (ESI/MS), was 39,213 Da which compares well with the theoretical mass (39,210.4 Da). Thrombin digestion of fusion protein was expected at a clone...

journal_title：Protein expression and purification

authors： Raftery MJ,Collinson L,Geczy CL

更新日期：1999-03-01 00:00:00

abstract：:Reticulons are a large family of integral membrane proteins that are ubiquitous in eukaryotes and play a key role in functional remodelling of the endoplasmic reticulum membrane. The reticulon family is especially large in plants, with the Arabidopsis thaliana genome containing twenty-one isoforms. Reticulons vary in ...

journal_title：Protein expression and purification

authors： Chow M,Sklepari M,Frigerio L,Dixon AM

更新日期：2018-12-01 00:00:00

abstract：:As an insect-selective neurotoxin, scorpion long-chain BjαIT is a promising prospect for insecticidal application; however, the difficulty of obtaining natural BjαIT represents the major obstacle preventing analysis of its insecticidal activity against agricultural insect pests. Here, we screened recombinant Pichia pa...

journal_title：Protein expression and purification

authors： Li H,Xia Y

更新日期：2018-12-01 00:00:00

abstract：:Hydrophobic charge-induction chromatography (HCIC) using 4-mercaptoethylpyridine (4-MEP) as the ligand is used to purify antibodies. The 4-MEP resin ligand has high affinity for antibodies, which makes it difficult to optimize the elution conditions. Recent studies showed that arginine is effective at eluting and puri...

journal_title：Protein expression and purification

authors： Hirano A,Maruyama T,Shiraki K,Arakawa T,Kameda T

更新日期：2017-01-01 00:00:00

abstract：:During the screening for tyrosine phenol-lyase-producing thermophiles, we isolated an obligatory symbiotic thermophile, Symbiobacterium sp. SC-1, which grew only in coculture with Bacillus sp. SK-1. A gene encoding thermostable tyrosine phenol-lyase (TPL) was cloned from the genomic DNA of the Symbiobacterium sp. SC-1...

journal_title：Protein expression and purification

authors： Lee SG,Hong SP,Choi YH,Chung YJ,Sung MH

更新日期：1997-12-01 00:00:00

abstract：:Human interleukin-7 (IL-7) is a member of the interleukin family. Numerous studies have demonstrated IL-7's effect on B- and T-cell development as well as its potential in various clinical applications. Previously, a study reported that IL-7 could be purified from inclusion bodies using a prokaryotic system, however, ...

journal_title：Protein expression and purification

authors： Luo Y,Kong X,Xu A,Jin S,Wu D

更新日期：2009-01-01 00:00:00

abstract：:We have investigated the suitability of Pichia pastoris as an expression system for the candidate therapeutic protein, Sonic hedgehog fused to an immunoglobulin Fc domain (Shh-Fc). Sonic hedgehog is a morphogen protein involved in the patterning of a wide range of tissues during animal embryogenesis. The presence of S...

journal_title：Protein expression and purification

authors： Shapiro RI,Wen D,Levesque M,Hronowski X,Gill A,Garber EA,Galdes A,Strauch KL,Taylor FR

更新日期：2003-06-01 00:00:00

abstract：:Cytosine-specific DNA methyltransferases are important enzymes in most living organisms. In prokaryotes, most DNA methyltransferases are members of the type II restriction-modification system where they methylate host DNA, thereby protecting it from digestion by the accompanying restriction endonucleases. DNA methyltr...

journal_title：Protein expression and purification

authors： Grgic M,Williamson A,Kjæreng Bjerga GE,Altermark B,Leiros I

更新日期：2018-10-01 00:00:00

abstract：:The human PRODH gene has been shown to have unique roles in regulating cell survival and apoptotic pathways and it has been related to velocardiofacial syndrome/DiGeorge syndrome and increased susceptibility to schizophrenia. It encodes for the flavoprotein proline oxidase (PO), which catalyzes the conversion of l-pro...

journal_title：Protein expression and purification

authors： Tallarita E,Pollegioni L,Servi S,Molla G

更新日期：2012-04-01 00:00:00

abstract：:The recombinant truncated endolysin LysK consisting of two catalytic domains, N-terminal CHAP and amidase-2 (LysKCA) was overexpressed in E. coli in the form of inclusion bodies (IBs). These IBs were dissolved in 6 M solution of urea followed by the refolding process. The refolding efficacy of the dilution and matrix-...

journal_title：Protein expression and purification

authors： Alaksandr Ž,Sergey G,Maksim P,Sergey K,Niyaz S,Uladzimir P,Mikhail S

更新日期：2020-10-01 00:00:00

abstract：:Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit epsilon N-methyltransferase (Protein methylase III, Rubisco LSMT, EC 2.1.1.43) catalyzes methylation of the epsilon-amino group of Lys-14 in the large subunit of Rubisco. In this paper, an affinity purification procedure for pea (Pisum sativum L. cv Laxton'...

journal_title：Protein expression and purification

authors： Wang P,Royer M,Houtz RL

更新日期：1995-08-01 00:00:00

abstract：:Strategies for the expression of precursors of eukaryotic secreted proteins as part of fused proteins in Escherichia coli have been explored. A fusion protein with beta-galactosidase at the N-terminal end and honeybee prepromelittin at the C-terminal end (beta-gal-pM) was expressed in low amounts as a cleaved polypept...

journal_title：Protein expression and purification

authors： He M,Adcock I,Chapman D,Lucy J,Austen B

更新日期：1991-10-01 00:00:00

abstract：:The AP-1 transcription factor is a dimeric protein complex formed primarily between Jun (c-Jun, JunB, JunD) and Fos (c-Fos, FosB, Fra-1, Fra-2) family members. These distinct AP-1 complexes are expressed in many cell types and modulate target gene expression implicated in cell proliferation, differentiation, and stres...

journal_title：Protein expression and purification

authors： Wang WM,Lee AY,Chiang CM

更新日期：2008-05-01 00:00:00