Abstract:
:We have constructed a plasmid, pAHOB1, with a 482-b AluI fragment containing the Escherichia coli glutaredoxin gene (grx) cloned under lambda PL promoter control. Growth of E. coli N4830/pAHOB1 cells at 30 degrees C followed by heat induction at 40 degrees C for 5 h resulted in expression of glutaredoxin as 20% of the soluble E. coli protein. Methods for the preparation of gram amounts of glutaredoxin and 5 mM solutions suitable for NMR studies were developed. About 10% of the glutaredoxin activity showed an unexpected higher isoelectric point and was isolated by DEAE-cellulose chromatography at pH 6.0. Sequence analysis demonstrated that this novel form (grx-90) contained five additional N-terminal residues (Met-Arg-Arg-Glu-Ile) added to the glutaredoxin molecule with 85 residues (grx-85). Grx-90 originates from an alternative ATG initiation codon present 5' of the previously identified translation start site on the grx gene in E. coli. Despite the highly charged N-terminal extension, grx-90 showed full activity as a GSH-disulfide oxidoreductase and the same apparent Km value (0.14 microM) as glutaredoxin in GSH-dependent reduction of CDP by ribonucleotide reductase. Both grx-90 and grx-85 showed identical competition curves in radioimmunoassays. The presence of grx-90 was also demonstrated in log-phase E. coli C600 cells as 5 to 10% of total glutaredoxin by immunological techniques. The molar extinction coefficient of native glutaredoxin (12,500 M-1 cm-1 at 280 nm) was 15% higher than expected from its content of one Trp and four Tyr residues.
journal_name
Protein Expr Purifjournal_title
Protein expression and purificationauthors
Björnberg O,Holmgren Adoi
10.1016/1046-5928(91)90085-wsubject
Has Abstractpub_date
1991-08-01 00:00:00pages
287-95issue
4eissn
1046-5928issn
1096-0279pii
1046-5928(91)90085-Wjournal_volume
2pub_type
杂志文章abstract::Bacteriocin, which is produced by lactic acid bacteria (LAB), has the potential to act as natural preservatives in the food industry. To develop strategies to overproduce such peptides, plantaricin NC8, a class IIb LAB bacteriocin that consists of two peptides, PLNC8α and PLNC8β, was successfully heterologously expres...
journal_title:Protein expression and purification
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journal_title:Protein expression and purification
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journal_title:Protein expression and purification
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journal_title:Protein expression and purification
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journal_title:Protein expression and purification
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journal_title:Protein expression and purification
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journal_title:Protein expression and purification
pub_type: 杂志文章
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journal_title:Protein expression and purification
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journal_title:Protein expression and purification
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journal_title:Protein expression and purification
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journal_title:Protein expression and purification
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journal_title:Protein expression and purification
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