Abstract:
:Individual cells in an organism are variable, which strongly impacts cellular processes. Advances in sequencing technologies have enabled single-cell genomic analysis to become widespread, addressing shortcomings of analyses conducted on populations of bulk cells. While the field of single-cell plant genomics is in its infancy, there is great potential to gain insights into cell lineage and functional cell types to help understand complex cellular interactions in plants. In this review, we discuss current approaches for single-cell plant genomic analysis, with a focus on single-cell isolation, DNA amplification, next-generation sequencing, and bioinformatics analysis. We outline the technical challenges of analysing material from a single plant cell, and then examine applications of single-cell genomics and the integration of this approach with genome editing. Finally, we indicate future directions we expect in the rapidly developing field of plant single-cell genomic analysis.
journal_name
Genes (Basel)journal_title
Genesauthors
Yuan Y,Lee H,Hu H,Scheben A,Edwards Ddoi
10.3390/genes9010050subject
Has Abstractpub_date
2018-01-22 00:00:00issue
1issn
2073-4425pii
genes9010050journal_volume
9pub_type
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