Engineered mesenchymal stem-cell-sheets patches prevents postoperative pancreatic leakage in a rat model.

Abstract:

:Post-operative pancreatic fistula (POPF) following pancreatic resection is a life-threatening surgical complication. Cell sheets were prepared and harvested using temperature-responsive culture dishes and transplanted as patches to seal POPF. Two different mesenchymal stem cell (MSC) sheets were compared in terms of the preventative ability for pancreatic leakage in a rat model. Both rat adipose-derived stem cell (rADSC) and bone marrow-derived stem cell (rBMSC) sheets were transplanted. Those rADSC and rBMSC sheets are created without enzymes and thus maintained their cell-cell junctions and adhesion proteins with intact fibronectin on the basal side, as well as characteristics of MSCs. The rats with post-pancreatectomy rADSC- or rBMSC-sheet patches had significantly decreased abdominal fluid leakage compared with the control group, demonstrated by MR image analysis and measurement of the volume of abdominal fluid. Amylase level was significantly lower in the rats with rADSC-sheet and rBMSC-sheet patches compared with the control groups. The rADSC sheet patches had increased adhesive and immune-cytokine profiles (ICAM-1, L-selectin, TIMP-1), and the rBMSC sheets had reduced immune reactions compared to the control. This is first project looking at the feasibility of tissue engineering therapy using MSC-sheets as tissue patches preventing leakage of abdominal fluid caused by POPF.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Kim SR,Yi HJ,Lee YN,Park JY,Hoffman RM,Okano T,Shim IK,Kim SC

doi

10.1038/s41598-017-18490-9

subject

Has Abstract

pub_date

2018-01-10 00:00:00

pages

360

issue

1

issn

2045-2322

pii

10.1038/s41598-017-18490-9

journal_volume

8

pub_type

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