Abstract：
:Genome engineering is a rapidly evolving field that benefits from the availability of different tools that can be used to perform genome manipulation tasks. We describe here the development of the Flp-TAL recombinases that can target genomic FRT-like sequences in their native chromosomal locations. Flp-TAL recombinases are hybrid enzymes that are composed of two functional modules: a variant of site-specific tyrosine recombinase Flp, which can have either narrow or broad target specificity, and the DNA-binding domain of the transcription activator-like effector, TAL. In Flp-TAL, the TAL module is responsible for delivering and stabilizing the Flp module onto the desired genomic FRT-like sequence where the Flp module mediates recombination. We demonstrate the functionality of the Flp-TAL recombinases by performing integration and deletion experiments in human HEK-293 cells. In the integration experiments we targeted a vector to three genomic FRT-like sequences located in the β-globin locus. In the deletion experiments we excised ~ 15 kilobases of DNA that contained a fragment of the integrated vector sequence and the neighboring genome sequence. On average, the efficiency of the integration and deletion reactions was about 0.1% and 20%, respectively.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Voziyanova E,Li F,Shah R,Voziyanov Ydoi
10.1038/s41598-020-74474-2subject
Has Abstractpub_date
2020-10-15 00:00:00pages
17479issue
1issn
2045-2322pii
10.1038/s41598-020-74474-2journal_volume
10pub_type
杂志文章abstract：:Huntington's disease (HD) is a progressive, dominantly inherited neurological disorder caused by an abnormal expansion of polyglutamine (polyQ) repeat within the Huntingtin (Htt) protein with no disease modifying treatments. In a Drosophila model of HD, expression of mutant Huntingtin (Htt) protein with expanded polyQ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep18736
更新日期：2016-01-05 00:00:00
abstract：:Perovskite LaFeO3/montmorillonite nanocomposites (LaFeO3/MMT) have been successfully prepared via assembling LaFeO3 nanoparticles on the surface of montmorillonite with citric acid assisted sol-gel method. The results indicated that the uniform LaFeO3 nanoparticles were densely deposited onto the surface of montmorill...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep19723
更新日期：2016-01-18 00:00:00
abstract：:The eastern North American migratory population of monarch butterflies (Danaus plexippus) is in serious decline. Habitat restoration, including adding millions of host plants to compensate for loss of milkweed in US cropland, is a key part of the international conservation strategy to return this iconic butterfly to s...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-30731-z
更新日期：2018-08-14 00:00:00
abstract：:The chorioallantoic-membrane (CAM)-assay is an established model for in vivo tumor research. Contrary to rodent-xenograft-models, the CAM-assay does not require breeding of immunodeficient strains due to native immunodeficiency. This allows xenografts to grow on the non-innervated CAM without pain or impairment for th...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-75660-y
更新日期：2020-10-29 00:00:00
abstract：:MicroRNA is responsible for the fine-tuning of fundamental cellular activities and human disease development. The altered availability of microRNAs, target mRNAs, and other types of endogenous RNAs competing for microRNA interactions reflects the dynamic and conditional property of microRNA-mediated gene regulation th...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-13470-5
更新日期：2017-10-17 00:00:00
abstract：:Experiments on the Ultimatum Game (UG) repeatedly show that people's behaviour is far from rational. In UG experiments, a subject proposes how to divide a pot and the other can accept or reject the proposal, in which case both lose everything. While rational people would offer and accept the minimum possible amount, i...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-19503-x
更新日期：2018-01-19 00:00:00
abstract：:Glaucoma is a leading cause of irreversible vision loss predicted to affect more than 100 million people by 2040. Intraocular pressure (IOP) reduction prevents development of glaucoma and vision loss from glaucoma. Glaucoma surgeries reduce IOP by facilitating aqueous humor outflow through a vent fashioned from the wa...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-69687-4
更新日期：2020-07-31 00:00:00
abstract：:Separation of CO2 from other gasses offers environmental benefits since CO2 gas is the main contributor to global warming. Recently, graphene oxide (GO) based gas separation membranes are of interest due to their selective barrier properties. However, maintaining selectivity without sacrificing permeance is still chal...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-00433-z
更新日期：2017-03-28 00:00:00
abstract：:Dogs (Canis familiaris) are the first animals to be domesticated by humans and the only ones domesticated by mobile hunter-gatherers. Wolves and humans were both persistent, pack hunters of large prey. They were species competing over resources in partially overlapping ecological niches and capable of killing each oth...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-78214-4
更新日期：2021-01-07 00:00:00
abstract：:Solitons, defined as nonlinear waves which can reflect from boundaries or transmit through each other, are found in conservative, fully integrable systems. Similar phenomena, dubbed quasi-solitons, have been observed also in dissipative, "excitable" systems, either at finely tuned parameters (near a bifurcation) or in...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep30879
更新日期：2016-08-05 00:00:00
abstract：:Inbred strains of mice are powerful models for understanding human pregnancy complications. For example, the exclusive mating of CBA/J females to DBA/2J males increases fetal resorption to 20-35% with an associated decline in placentation and maintenance of maternal Th1 immunity. More recently other complications of p...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep35138
更新日期：2016-10-21 00:00:00
abstract：:Application of metabolic magnetic resonance imaging measures such as cerebral blood flow in translational medicine is limited by the unknown link of observed alterations to specific neurophysiological processes. In particular, the sensitivity of cerebral blood flow to activity changes in specific neurotransmitter syst...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-22444-0
更新日期：2018-03-06 00:00:00
abstract：:Glioblastoma multiforme (GBM) is a highly invasive and chemoradioresistant brain malignancy. Temozolomide (TMZ), a DNA-alkylating agent, is effective against GBM and has become the standard first-line drug. However, the mechanism by which TMZ regulates the progression of GBM remains elusive. Here, we demonstrate that ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep01160
更新日期：2013-01-01 00:00:00
abstract：:The micro-mechanisms of brittle failure affect the bulk mechanical behaviour and permeability of crustal rocks. In low-porosity crystalline rocks, these mechanisms are related to mineralogy and fabric anisotropy, while confining pressure, temperature and strain rates regulate the transition from brittle to ductile beh...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-15523-1
更新日期：2017-11-10 00:00:00
abstract：:In this paper, we study the influence of the fingerprint and sweat on the fingerprint on the friction between the hand and an object. When sweat contacts a finger or an object, it is sometimes easy to pick up the object. In particular, we can see this phenomenon when grasping a thin object such as paper and vinyl. The...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-51694-9
更新日期：2019-11-12 00:00:00
abstract：:Comparable taxonomic ranks within clades can facilitate more consistent classifications and objective comparisons among taxa. Here we use a temporal approach to identify taxonomic ranks. This is an extension of the temporal banding approach including a Temporal Error Score that finds an objective cut-off for each taxo...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-02477-7
更新日期：2017-05-23 00:00:00
abstract：:Many studies have revealed the cognitive decline induced by microwave radiation. However, the systematic study on dose-dependent, frequency-dependent and accumulative effects of microwave exposure at different frequencies was lacking. Here, we studied the relationship between the effects and the power and frequency of...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-11420-9
更新日期：2017-09-07 00:00:00
abstract：:Multisensory interactions are essential to make sense of the environment by transforming the mosaic of sensory inputs received by the organism into a unified perception. Brain rhythms allow coherent processing within areas or between distant brain regions and could thus be instrumental in functionally connecting remot...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-47503-y
更新日期：2019-08-02 00:00:00
abstract：:Disc degeneration (DD) is a multifaceted chronic process that alters the structure and function of intervertebral discs. The pathophysiology of degeneration is not completely understood, but the consensus is that changes in genes encoding extracellular matrix (ECM) proteins in the disc are the leading factors contribu...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-04384-3
更新日期：2017-06-23 00:00:00
abstract：:Soil zinc (Zn) plays a crucial role in plant growth, but excessive accumulation in the environment may lead to air, water and soil pollution. It is affected by various chemical, environmental and spatial factors. Therefore, it is important to identify the factors influencing Zn content in the landscape. The main motiv...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-33745-9
更新日期：2018-10-22 00:00:00
abstract：:Nanocrystals of Ln3+ (Ln = Yb, Tm and Ho) doped β-NaLuF4 with average diameter about 200 nm are dispersed in silica-based microtube (MT) by a simple flame heating method. The fabricated microtube has a diameter range from 2 μm to 30 μm and lengths up to hundreds microns. The fluorescence of upconversion nanocrystals (...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep35941
更新日期：2016-10-25 00:00:00
abstract：:Most traits of agricultural importance are quantitative traits controlled by numerous genes. However, it remains unclear about the molecular mechanisms underpinning quantitative traits. Here, we report the molecular characteristics of the genes controlling three quantitative traits randomly selected from three diverse...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-66271-8
更新日期：2020-06-22 00:00:00
abstract：:Spintronic devices currently rely on magnetic switching or controlled motion of domain walls by an external magnetic field or spin-polarized current. Achieving the same degree of magnetic controllability using an electric field has potential advantages including enhanced functionality and low power consumption. Here w...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep00258
更新日期：2012-01-01 00:00:00
abstract：:The Goos-Hänchen (GH) shifts in the reflected light are investigated both for p and s polarized partial coherent light beams incident on epsilon-near-zero (ENZ) metamaterials. In contrary to the coherent counterparts, the magnitude of GH shift becomes non-zero for p polarized partial coherent light beam; while GH shif...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep26504
更新日期：2016-05-23 00:00:00
abstract：:Multidrug resistance (MDR) is a major impediment to cancer treatment. Here, for the first time, we investigated the chemo-sensitizing effect of Noscapine (Nos) at low concentrations in conjunction with docetaxel (DTX) to overcome drug resistance of triple negative breast cancer (TNBC). In vitro experiments showed that...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-15531-1
更新日期：2017-11-20 00:00:00
abstract：:To date, there is only scarce evidence for a considerable association of subjective and objective stress measures, which might be attributable to method bias (e.g., confounding) and/or asynchrony of their temporal changes. To validate different subjective stress measures by a physiological measure of long-term stress ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-37526-2
更新日期：2019-01-31 00:00:00
abstract：:Energy transfer upconversion (ETU) is known to be the most efficient frequency upconversion mechanism. Surface plasmon can further enhance the upconversion process, opening doors to many applications. However, ETU is a complex process involving competing transitions between multiple energy levels and it has been diffi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep18894
更新日期：2016-01-07 00:00:00
abstract：:Phages infecting Aeromonas salmonicida subsp. salmonicida, the causative agent of the fish disease furunculosis, have been isolated for decades but very few of them have been characterized. Here, the host range of 12 virulent phages, including three isolated in the present study, was evaluated against a panel of 65 A....
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-07401-7
更新日期：2017-08-01 00:00:00
abstract：:Deformable elastomeric spheres are evaluated experimentally as they skip multiple times over a lake surface. Some spheres are embedded with small inertial measurement units to measure the acceleration experienced during water surface impact. A model for multiple impact events shows good agreement between measured acce...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-42453-x
更新日期：2019-04-15 00:00:00
abstract：:Immunotherapeutic strategies for glioblastoma, the most frequent malignant primary brain tumor, aim to improve its disastrous consequences. On top of the standard treatment, one strategy uses T cell activation by autologous dendritic cells (DC) ex vivo loaded with tumor lysate to attack remaining cancer cells. Wonderi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-12584-0
更新日期：2017-10-24 00:00:00
