Abstract：
Objective:The role of growth factors, including vascular endothelial growth factor of activated omentum on mitosis is clearly known, though not on all the aspects of in vitro oocyte maturation. This study was designed to assess the effect of activated-omental extract (AOE) on in vitro maturation (IVM) of rat cumulus-oocyte complexes (COCs). Materials and Methods:In this experimental study, the COCs were incubated in Ham's F-10 supplemented with either 20% AOE, 20% fetal bovine serum (FBS) or serum-free media. Post-culture COCs were studied according to the cumulus cells (CCs) expansion, nuclear maturation and cytoplasmic maturation. Cumuli expansion was evaluated by inverted microscope without staining; nuclear maturation was assessed by aceto-orcein staining (light microscope) and cytoplasmic maturation was also observed by TEM. Results:Expansion of CCs and nuclear maturation of the oocytes in in vitro for 24 hr was significantly higher in AOE- and FBS-supplemented groups (P=0.000 and 0.013) and (P=0.004 and 0.014), respectively, compared to serum-free group. At ultra-structural level, after 24 hr, both FBS and AOE-supplemented media showed uniformly wide perivitelline space (PVS). After 12 hr, the cortical granules were found in the oocytes cultured in FBS and AOE-supplemented media. Within 24 hr, both granules and mitochondria were large without any detectable topographic tendency across the ooplasm. In AOE and FBS-supplemented oocytes, the number and size of microvilli were more than those in serum-free one. Conclusion:Although AOE supplementation induced a higher rate of the CCs expansion, and resuming meiosis, it was not as potent as FBS to provide cytoplasmic maturation of rat oocytes.
journal_name
Iran J Basic Med Scijournal_title
Iranian journal of basic medical sciencesauthors
Mesbah F,Pracha AD,Talaei-Khozani T,Bahmanpour Sdoi
10.22038/IJBMS.2017.9622subject
Has Abstractpub_date
2017-12-01 00:00:00pages
1345-1353issue
12eissn
2008-3866issn
2008-3874pii
IJBMS-20-1345journal_volume
20pub_type
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journal_title：Iranian journal of basic medical sciences
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journal_title：Iranian journal of basic medical sciences
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doi：
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