当前位置： SCI文献检索 > Scientific Reports期刊下所有文献 > Addition of a histone deacetylase inhibitor increases recombinant protein expression in Medicago truncatula cell cultures.

Addition of a histone deacetylase inhibitor increases recombinant protein expression in Medicago truncatula cell cultures.

Abstract：

:Plant cell cultures are an attractive platform for the production of recombinant proteins. A major drawback, hindering the establishment of plant cell suspensions as an industrial platform, is the low product yield obtained thus far. Histone acetylation is associated with increased transcription levels, therefore it is expected that the use of histone deacetylase inhibitors would result in an increase in mRNA and protein levels. Here, this hypothesis was tested by adding a histone deacetylase inhibitor, suberanilohydroxamic acid (SAHA), to a cell line of the model legume Medicago truncatula expressing a recombinant human protein. Histone deacetylase inhibition by SAHA and histone acetylation levels were studied, and the effect of SAHA on gene expression and recombinant protein levels was assessed by digital PCR. SAHA addition effectively inhibited histone deacetylase activity resulting in increased histone acetylation. Higher levels of transgene expression and accumulation of the associated protein were observed. This is the first report describing histone deacetylase inhibitors as inducers of recombinant protein expression in plant cell suspensions as well as the use of digital PCR in these biological systems. This study paves the way for employing epigenetic strategies to improve the final yields of recombinant proteins produced by plant cell cultures.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Santos RB,Pires AS,Abranches R

doi

10.1038/s41598-017-17006-9

subject

Has Abstract

pub_date

2017-12-01 00:00:00

pages

16756

issue

1

issn

2045-2322

pii

10.1038/s41598-017-17006-9

journal_volume

7

pub_type

杂志文章

相关文献

Scientific Reports文献大全
  • Postmenopausal Osteoporosis reference genes for qPCR expression assays.

    abstract：:Osteoporosis (OP) is a multifactorial disease influenced by genetic factors in more than half of the cases. In spite of the efforts to clarify the relationship among genetic factors and susceptibility to develop OP, many genetic associations need to be further functionally validated. Besides, some limitations as the c...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-52612-9

    authors： de Lima CAD,de Lima SC,Barbosa AD,Sandrin-Garcia P,de Barros Pita W,de Azevêdo Silva J,Crovella S

    更新日期：2019-11-11 00:00:00

  • A novel therapeutic approach using peripheral blood mononuclear cells preconditioned by oxygen-glucose deprivation.

    abstract：:Cell therapies that invoke pleiotropic mechanisms may facilitate functional recovery in patients with stroke. Based on previous experiments using microglia preconditioned by oxygen-glucose deprivation, we hypothesized that the administration of peripheral blood mononuclear cells (PBMCs) preconditioned by oxygen-glucos...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-53418-5

    authors： Hatakeyama M,Kanazawa M,Ninomiya I,Omae K,Kimura Y,Takahashi T,Onodera O,Fukushima M,Shimohata T

    更新日期：2019-11-14 00:00:00

  • Temporal changes in the radiocesium distribution in forests over the five years after the Fukushima Daiichi Nuclear Power Plant accident.

    abstract：:To elucidate the temporal changes in the radiocesium distribution in forests contaminated by the Fukushima Daiichi Nuclear Power Plant accident, we monitored the 137Cs concentration and inventory within forests from 2011 to 2015 across nine plots containing variable tree species and different contamination levels. The...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-08261-x

    authors： Imamura N,Komatsu M,Ohashi S,Hashimoto S,Kajimoto T,Kaneko S,Takano T

    更新日期：2017-08-15 00:00:00

  • Brillouin scattering-induced rogue waves in self-pulsing fiber lasers.

    abstract：:We report the experimental observation of extreme instabilities in a self-pulsing fiber laser under the influence of stimulated Brillouin scattering (SBS). Specifically, we observe temporally localized structures with high intensities that can be referred to as rogue events through their statistical behaviour with hig...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep45868

    authors： Hanzard PH,Talbi M,Mallek D,Kellou A,Leblond H,Sanchez F,Godin T,Hideur A

    更新日期：2017-04-04 00:00:00

  • Inferences of drug responses in cancer cells from cancer genomic features and compound chemical and therapeutic properties.

    abstract：:Accurately predicting the response of a cancer patient to a therapeutic agent is a core goal of precision medicine. Existing approaches were mainly relied primarily on genomic alterations in cancer cells that have been treated with different drugs. Here we focus on predicting drug response based on integration of the ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep32679

    authors： Wang Y,Fang J,Chen S

    更新日期：2016-09-20 00:00:00

  • Combinatorial metabolic engineering of Pseudomonas putida KT2440 for efficient mineralization of 1,2,3-trichloropropane.

    abstract：:An industrial waste, 1,2,3-trichloropropane (TCP), is toxic and extremely recalcitrant to biodegradation. To date, no natural TCP degraders able to mineralize TCP aerobically have been isolated. In this work, we engineered a biosafety Pseudomonas putida strain KT2440 for aerobic mineralization of TCP by implantation o...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-07435-x

    authors： Gong T,Xu X,Che Y,Liu R,Gao W,Zhao F,Yu H,Liang J,Xu P,Song C,Yang C

    更新日期：2017-08-01 00:00:00

  • Nafamostat and sepimostat identified as novel neuroprotective agents via NR2B N-methyl-D-aspartate receptor antagonism using a rat retinal excitotoxicity model.

    abstract：:In addition to its role in the treatment of pancreatitis, the serine protease inhibitor nafamostat exhibits a retinal protective effect. However, the exact mechanisms underlying this effect are unknown. In this study, the neuroprotective effects of nafamostat and its orally active derivative sepimostat against excitot...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-56905-x

    authors： Fuwa M,Kageyama M,Ohashi K,Sasaoka M,Sato R,Tanaka M,Tashiro K

    更新日期：2019-12-31 00:00:00

  • A rationale for surgical debulking to improve anti-PD1 therapy outcome in non small cell lung cancer.

    abstract：:Anti-PD1 immunotherapy has emerged as a gold-standard treatment for first- or second-line treatment of stage IV NSCLC, with response rates ranging from 10 to 60%. Strategies to improve the disease control rate are needed. Several reports suggested that debulking surgery enhances anti-tumor immunity. We aimed at examin...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-52913-z

    authors： Guisier F,Cousse S,Jeanvoine M,Thiberville L,Salaun M

    更新日期：2019-11-15 00:00:00

  • Response of aboveground biomass and diversity to nitrogen addition - a five-year experiment in semi-arid grassland of Inner Mongolia, China.

    abstract：:Understanding the response of the plant community to increasing nitrogen (N) deposition is helpful for improving pasture management in semi-arid areas. We implemented a 5-year N addition experiment in a Stipa krylovii steppe of Inner Mongolia, northern China. The aboveground biomass (AGB) and species richness were mea...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep31919

    authors： He K,Qi Y,Huang Y,Chen H,Sheng Z,Xu X,Duan L

    更新日期：2016-08-30 00:00:00

  • A Semi-Continuous Process For Polyphenols Extraction From Sea Buckthorn Leaves.

    abstract：:Sea buckthorn (Hippophae Rhamnoides L.) is an important source of bioactive compounds such as: antioxidants, vitamins, fatty acids, amino acids, and minerals. Sea buckthorn leaves extracts have been proved to have anti-microbial, antioxidant, anti-inflammatory, and anti-viral properties. In this paper, the extraction ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-48610-6

    authors： Asofiei I,Calinescu I,Trifan A,Gavrila AI

    更新日期：2019-08-19 00:00:00

  • Acclimation of bacterial cell state for high-throughput enzyme engineering using a DmpR-dependent transcriptional activation system.

    abstract：:Genetic circuit-based biosensors have emerged as an effective analytical tool in synthetic biology; these biosensors can be applied to high-throughput screening of new biocatalysts and metabolic pathways. Sigma 54 (σ54)-dependent transcription factor (TF) can be a valuable component of these biosensors owing to its in...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-62892-1

    authors： Kwon KK,Yeom SJ,Choi SL,Rha E,Lee H,Kim H,Lee DH,Lee SG

    更新日期：2020-04-08 00:00:00

  • Mechanistic insight into the substrate specificity of 1,2-β-oligoglucan phosphorylase from Lachnoclostridium phytofermentans.

    abstract：:Glycoside phosphorylases catalyze the phosphorolysis of oligosaccharides into sugar phosphates. Recently, we found a novel phosphorylase acting on β-1,2-glucooligosaccharides with degrees of polymerization of 3 or more (1,2-β-oligoglucan phosphorylase, SOGP) in glycoside hydrolase family (GH) 94. Here, we characterize...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep42671

    authors： Nakajima M,Tanaka N,Furukawa N,Nihira T,Kodutsumi Y,Takahashi Y,Sugimoto N,Miyanaga A,Fushinobu S,Taguchi H,Nakai H

    更新日期：2017-02-15 00:00:00

  • 26Al/10Be Burial Dating of the Middle Pleistocene Yiyuan Hominin Fossil Site, Shandong Province, Northern China.

    abstract：:The Yiyuan hominin fossil site is one of the few localities in China where a partial skullcap and several loose teeth of Homo erectus have been discovered. Yiyuan was previously assigned broadly to the Middle Pleistocene by biostratigraphical correlation and ESR/U-series dating. Here, we report the first application o...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-43401-5

    authors： Guo Y,Sun C,Luo L,Yang L,Han F,Tu H,Lai Z,Jiang H,Bae CJ,Shen G,Granger D

    更新日期：2019-05-06 00:00:00

  • Alpha-synuclein prevents the formation of spherical mitochondria and apoptosis under oxidative stress.

    abstract：:Oxidative stress (OS), mitochondrial dysfunction, and dysregulation of alpha-synuclein (aSyn) homeostasis are key pathogenic factors in Parkinson's disease. Nevertheless, the role of aSyn in mitochondrial physiology remains elusive. Thus, we addressed the impact of aSyn specifically on mitochondrial response to OS in ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep42942

    authors： Menges S,Minakaki G,Schaefer PM,Meixner H,Prots I,Schlötzer-Schrehardt U,Friedland K,Winner B,Outeiro TF,Winklhofer KF,von Arnim CA,Xiang W,Winkler J,Klucken J

    更新日期：2017-02-22 00:00:00

  • TGF-β induces phosphorylation of phosphatase and tensin homolog: implications for fibrosis of the trabecular meshwork tissue in glaucoma.

    abstract：:Fundamental cell signaling mechanisms that regulate dynamic remodeling of the extracellular matrix (ECM) in mechanically loaded tissues are not yet clearly understood. Trabecular meshwork (TM) tissue in the eye is under constant mechanical stress and continuous remodeling of ECM is crucial to maintain normal aqueous h...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-00845-x

    authors： Tellios N,Belrose JC,Tokarewicz AC,Hutnik C,Liu H,Leask A,Motolko M,Iijima M,Parapuram SK

    更新日期：2017-04-11 00:00:00

  • Ice-core evidence of earliest extensive copper metallurgy in the Andes 2700 years ago.

    abstract：:The importance of metallurgy for social and economic development is indisputable. Although copper (Cu) was essential for the wealth of pre- and post-colonial societies in the Andes, the onset of extensive Cu metallurgy in South America is still debated. Comprehensive archaeological findings point to first sophisticate...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep41855

    authors： Eichler A,Gramlich G,Kellerhals T,Tobler L,Rehren T,Schwikowski M

    更新日期：2017-01-31 00:00:00

  • Control of decoherence with no control.

    abstract：:A common philosophy in control theory is the control of disorder by order. Control of decoherence is no exception; strategies aimed at suppressing quantum decoherence adopt this point of view. Here we predict an anomalous phenomenon in open quantum systems-control of disorder by (even more) disorder. It is shown that ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep02746

    authors： Jing J,Wu LA

    更新日期：2013-09-25 00:00:00

  • Superb electromagnetic wave-absorbing composites based on large-scale graphene and carbon nanotube films.

    abstract：ABSTARCT:Graphene has sparked extensive research interest for its excellent physical properties and its unique potential for application in absorption of electromagnetic waves. However, the processing of stable large-scale graphene and magnetic particles on a micrometer-thick conductive support is a formidable challeng...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-02639-7

    authors： Li J,Lu W,Suhr J,Chen H,Xiao JQ,Chou TW

    更新日期：2017-05-24 00:00:00

  • Nanoscale Spatially Resolved Mapping of Uranium Enrichment.

    abstract：:Spatially resolved analysis of uranium (U) isotopes in small volumes of actinide-bearing materials is critical for a variety of technical disciplines, including earth and planetary sciences, environmental monitoring, bioremediation, and the nuclear fuel cycle. However, achieving subnanometer-scale spatial resolution f...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-48479-5

    authors： Kautz E,Burkes D,Joshi V,Lavender C,Devaraj A

    更新日期：2019-08-23 00:00:00

  • Membrane depolarization and aberrant lipid distributions in the neonatal rat brain following hypoxic-ischaemic insult.

    abstract：:Neonatal hypoxic-ischaemic (HI) encephalopathy is among the most serious complications in neonatology. In the present study, we studied the immediate (0 hour), subacute (36 hours) and late (144 hours) responses of the neonatal brain to experimental HI insult in laboratory rats. At the striatal level, the mass spectrom...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-25088-2

    authors： Luptakova D,Baciak L,Pluhacek T,Skriba A,Sediva B,Havlicek V,Juranek I

    更新日期：2018-05-03 00:00:00

  • Local entrainment of oscillatory activity induced by direct brain stimulation in humans.

    abstract：:In a quest for direct evidence of oscillation entrainment, we analyzed intracerebral electroencephalographic recordings obtained during intracranial electrical stimulation in a cohort of three medication-resistant epilepsy patients tested pre-surgically. Spectral analyses of non-epileptogenic cerebral sites stimulated...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep41908

    authors： Amengual JL,Vernet M,Adam C,Valero-Cabré A

    更新日期：2017-03-03 00:00:00

  • Moyamoya disease-associated protein mysterin/RNF213 is a novel AAA+ ATPase, which dynamically changes its oligomeric state.

    abstract：:Moyamoya disease is an idiopathic human cerebrovascular disorder that is characterized by progressive stenosis and abnormal collateral vessels. We recently identified mysterin/RNF213 as its first susceptibility gene, which encodes a 591-kDa protein containing enzymatically active P-loop ATPase and ubiquitin ligase dom...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep04442

    authors： Morito D,Nishikawa K,Hoseki J,Kitamura A,Kotani Y,Kiso K,Kinjo M,Fujiyoshi Y,Nagata K

    更新日期：2014-03-24 00:00:00

  • Inverted optical intrinsic response accompanied by decreased cerebral blood flow are related to both neuronal inhibition and excitation.

    abstract：:Negative hemodynamic response has been widely reported in blood oxygenation level-dependent (BOLD) functional magnetic resonance imaging studies, however its origin is still controversial. Optical intrinsic signal (OIS) imaging can be used to study brain activity by simultaneously recording hemodynamic signals at diff...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep21627

    authors： Ma Z,Cao P,Sun P,Zhao L,Li L,Tong S,Lu Y,Yan Y,Chen Y,Chai X

    更新日期：2016-02-10 00:00:00

  • Isolation and no-entry marine reserves mitigate anthropogenic impacts on grey reef shark behavior.

    abstract：:Reef sharks are vulnerable predators experiencing severe population declines mainly due to overexploitation. However, beyond direct exploitation, human activities can produce indirect or sub-lethal effects such as behavioral alterations. Such alterations are well known for terrestrial fauna but poorly documented for m...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-37145-x

    authors： Juhel JB,Vigliola L,Wantiez L,Letessier TB,Meeuwig JJ,Mouillot D

    更新日期：2019-02-27 00:00:00

  • KvN mechanics approach to the time-dependent frequency harmonic oscillator.

    abstract：:Using the Ermakov-Lewis invariants appearing in KvN mechanics, the time-dependent frequency harmonic oscillator is studied. The analysis builds upon the operational dynamical model, from which it is possible to infer quantum or classical dynamics; thus, the mathematical structure governing the evolution will be the sa...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-26759-w

    authors： Ramos-Prieto I,Urzúa-Pineda AR,Soto-Eguibar F,Moya-Cessa HM

    更新日期：2018-05-30 00:00:00

  • Discrimination of Transgenic Rice containing the Cry1Ab Protein using Terahertz Spectroscopy and Chemometrics.

    abstract：:Spectroscopic techniques combined with chemometrics methods have proven to be effective tools for the discrimination of objects with similar properties. In this work, terahertz time-domain spectroscopy (THz-TDS) combined with discriminate analysis (DA) and principal component analysis (PCA) with derivative pretreatmen...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep11115

    authors： Xu W,Xie L,Ye Z,Gao W,Yao Y,Chen M,Qin J,Ying Y

    更新日期：2015-07-08 00:00:00

  • Electrochemical monitoring of an important biomarker and target protein: VEGFR2 in cell lysates.

    abstract：:Vascular endothelial growth factor receptor 2 (VEGFR2) is a potential cell-type biomarker in clinical diagnoses. Besides, it's the target protein of many tyrosine kinase inhibitors and its expression significantly associates with clinical performance of these inhibitors. VEGFR2 detection provides an early warning for ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep03982

    authors： Wei T,Tu W,Zhao B,Lan Y,Bao J,Dai Z

    更新日期：2014-02-05 00:00:00

  • Rethinking food anticipatory activity in the activity-based anorexia rat model.

    abstract：:When a rat is on a limited fixed-time food schedule with full access to a running wheel (activity-based anorexia model, ABA), its activity level will increase hours prior to the feeding period. This activity, called food-anticipatory activity (FAA), is a hypothesized parallel to the hyperactivity symptom in human anor...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep03929

    authors： Wu H,van Kuyck K,Tambuyzer T,Luyten L,Aerts JM,Nuttin B

    更新日期：2014-01-29 00:00:00

  • In vivo Real-Time Mass Spectrometry for Guided Surgery Application.

    abstract：:Here we describe a new instrument (SpiderMass) designed for in vivo and real-time analysis. In this instrument ion production is performed remotely from the MS instrument and the generated ions are transported in real-time to the MS analyzer. Ion production is promoted by Resonant Infrared Laser Ablation (RIR-LA) base...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep25919

    authors： Fatou B,Saudemont P,Leblanc E,Vinatier D,Mesdag V,Wisztorski M,Focsa C,Salzet M,Ziskind M,Fournier I

    更新日期：2016-05-18 00:00:00

  • Relationship between social support during pregnancy and postpartum depressive state: a prospective cohort study.

    abstract：:Although the association between social support and postpartum depression has been previously investigated, its causal relationship remains unclear. Therefore, we examined prospectively whether social support during pregnancy affected postpartum depression. Social support and depressive symptoms were assessed by Japan...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep10520

    authors： Morikawa M,Okada T,Ando M,Aleksic B,Kunimoto S,Nakamura Y,Kubota C,Uno Y,Tamaji A,Hayakawa N,Furumura K,Shiino T,Morita T,Ishikawa N,Ohoka H,Usui H,Banno N,Murase S,Goto S,Kanai A,Masuda T,Ozaki N

    更新日期：2015-05-29 00:00:00