Injection of high dose botulinum-toxin A leads to impaired skeletal muscle function and damage of the fibrilar and non-fibrilar structures.

Abstract:

:Botulinum-toxin A (BoNT/A) is used for a wide range of conditions. Intramuscular administration of BoNT/A inhibits the release of acetylcholine at the neuromuscular junction from presynaptic motor neurons causing muscle-paralysis. The aim of the present study was to investigate the effect of high dose intramuscular BoNT/A injections (6 UI = 60 pg) on muscle tissue. The gait pattern of the rats was significantly affected 3 weeks after BoNT/A injection. The ankle joint rotated externally, the rats became flat footed, and the stride length decreased after BoNT/A injection. Additionally, there was clear evidence of microstructural changes on the tissue level by as evidenced by 3D imaging of the muscles by Synchrotron Radiation X-ray Tomographic Microscopy (SRXTM). Both the fibrillar and the non-fibrillar tissues were affected. The volume fraction of fibrillary tissue was reduced significantly and the non-fibrillar tissue increased. This was accompanied by a loss of the linear structure of the muscle tissue. Furthermore, gene expression analysis showed a significant upregulation of COL1A1, MMP-2, TGF-b1, IL-6, MHCIIA and MHCIIx in the BoNT/A injected leg, while MHVIIB was significantly downregulated. IN CONCLUSION:The present study reveals that high dose intramuscular BoNT/A injections cause microstructural damage of the muscle tissue, which contributes to impaired gait.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Pingel J,Nielsen MS,Lauridsen T,Rix K,Bech M,Alkjaer T,Andersen IT,Nielsen JB,Feidenhansl R

doi

10.1038/s41598-017-14997-3

subject

Has Abstract

pub_date

2017-11-07 00:00:00

pages

14746

issue

1

issn

2045-2322

pii

10.1038/s41598-017-14997-3

journal_volume

7

pub_type

杂志文章
  • Magnetic-flux-driven topological quantum phase transition and manipulation of perfect edge states in graphene tube.

    abstract::We study the tight-binding model for a graphene tube with perimeter N threaded by a magnetic field. We show exactly that this model has different nontrivial topological phases as the flux changes. The winding number, as an indicator of topological quantum phase transition (QPT) fixes at N/3 if N/3 equals to its intege...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep31953

    authors: Lin S,Zhang G,Li C,Song Z

    更新日期:2016-08-24 00:00:00

  • Long-term surgical outcomes of primary retropupillary iris claw intraocular lens implantation for the treatment of intraocular lens dislocation.

    abstract::We aimed to investigate the efficacy and safety of primary retropupillary iris claw intraocular lens (R-IOL) implantation in patients with complete intraocular lens (IOL) dislocation. In this single-center retrospective case series, we reviewed the medical records of patients who underwent R-IOL implantation surgery w...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-80292-3

    authors: Choi EY,Lee CH,Kang HG,Han JY,Byeon SH,Kim SS,Koh HJ,Kim M

    更新日期:2021-01-12 00:00:00

  • Evaluation of hyperspectral LiDAR for monitoring rice leaf nitrogen by comparison with multispectral LiDAR and passive spectrometer.

    abstract::Fast and nondestructive assessment of leaf nitrogen concentration (LNC) is critical for crop growth diagnosis and nitrogen management guidance. In the last decade, multispectral LiDAR (MSL) systems have promoted developments in the earth and ecological sciences with the additional spectral information. With more wavel...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep40362

    authors: Sun J,Shi S,Gong W,Yang J,Du L,Song S,Chen B,Zhang Z

    更新日期:2017-01-16 00:00:00

  • Publisher Correction: Mass & secondary structure propensity of amino acids explain their mutability and evolutionary replacements.

    abstract::A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper. ...

    journal_title:Scientific reports

    pub_type: 已发布勘误

    doi:10.1038/s41598-018-21981-y

    authors: Bohórquez HJ,Suárez CF,Patarroyo ME

    更新日期:2018-03-06 00:00:00

  • A biofilm and organomineralisation model for the growth and limiting size of ooids.

    abstract::Ooids are typically spherical sediment grains characterised by concentric layers encapsulating a core. There is no universally accepted explanation for ooid genesis, though factors such as agitation, abiotic and/or microbial mineralisation and size limitation have been variously invoked. Here we examine the possible i...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-18908-4

    authors: Batchelor MT,Burne RV,Henry BI,Li F,Paul J

    更新日期:2018-01-11 00:00:00

  • HIV Subtypes B and C gp120 and Methamphetamine Interaction: Dopaminergic System Implicates Differential Neuronal Toxicity.

    abstract::HIV subtypes or clades differentially induce HIV-associated neurocognitive disorders (HAND) and substance abuse is known to accelerate HIV disease progression. The HIV-1 envelope protein gp120 plays a major role in binding and budding in the central nervous system (CNS) and impacts dopaminergic functions. However, the...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep11130

    authors: Samikkannu T,Rao KVK,Salam AAA,Atluri VSR,Kaftanovskaya EM,Agudelo M,Perez S,Yoo C,Raymond AD,Ding H,Nair MPN

    更新日期:2015-06-09 00:00:00

  • Adenoviral E4 34K protein interacts with virus packaging components and may serve as the putative portal.

    abstract::Studies on dsDNA bacteriophages have revealed that a DNA packaging complex assembles at a special vertex called the 'portal vertex' and consists of a portal, a DNA packaging ATPase and other components. AdV protein IVa2 is presumed to function as a DNA packaging ATPase. However, a protein that functions as a portal is...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-07997-w

    authors: Ahi YS,Hassan AO,Vemula SV,Li K,Jiang W,Zhang GJ,Mittal SK

    更新日期:2017-08-08 00:00:00

  • Tethered primary hepatocyte spheroids on polystyrene multi-well plates for high-throughput drug safety testing.

    abstract::Hepatocyte spheroids are useful models for mimicking liver phenotypes in vitro because of their three-dimensionality. However, the lack of a biomaterial platform which allows the facile manipulation of spheroid cultures on a large scale severely limits their application in automated high-throughput drug safety testing...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-61699-4

    authors: Tasnim F,Singh NH,Tan EKF,Xing J,Li H,Hissette S,Manesh S,Fulwood J,Gupta K,Ng CW,Xu S,Hill J,Yu H

    更新日期:2020-03-16 00:00:00

  • Structure Shapes Dynamics and Directionality in Diverse Brain Networks: Mathematical Principles and Empirical Confirmation in Three Species.

    abstract::Identifying how spatially distributed information becomes integrated in the brain is essential to understanding higher cognitive functions. Previous computational and empirical studies suggest a significant influence of brain network structure on brain network function. However, there have been few analytical approach...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep46606

    authors: Moon JY,Kim J,Ko TW,Kim M,Iturria-Medina Y,Choi JH,Lee J,Mashour GA,Lee U

    更新日期:2017-04-20 00:00:00

  • Birth size and gestational age in opposite-sex twins as compared to same-sex twins: An individual-based pooled analysis of 21 cohorts.

    abstract::It is well established that boys are born heavier and longer than girls, but it remains unclear whether birth size in twins is affected by the sex of their co-twin. We conducted an individual-based pooled analysis of 21 twin cohorts in 15 countries derived from the COllaborative project of Development of Anthropometri...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-24634-2

    authors: Jelenkovic A,Sund R,Yokoyama Y,Hur YM,Ullemar V,Almqvist C,Magnusson PK,Willemsen G,Bartels M,Beijsterveldt CEV,Bogl LH,Pietiläinen KH,Vuoksimaa E,Ji F,Ning F,Pang Z,Nelson TL,Whitfield KE,Rebato E,Llewellyn CH,Fi

    更新日期:2018-04-19 00:00:00

  • An ultraviolet responsive hybrid solar cell based on titania/poly(3-hexylthiophene).

    abstract::Here we present an ultraviolet responsive inorganic-organic hybrid solar cell based on titania/poly(3-hexylthiophene) (TiO(2)/P3HT) heterojuction. In this solar cell, TiO(2) is an ultraviolet light absorber and electronic conductor, P3HT is a hole conductor, the light-to-electrical conversion is realized by the cooper...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep01283

    authors: Wu J,Yue G,Xiao Y,Lin J,Huang M,Lan Z,Tang Q,Huang Y,Fan L,Yin S,Sato T

    更新日期:2013-01-01 00:00:00

  • Importance of Experimental Environmental Conditions in Estimating Risks and Associated Uncertainty of Transgenic Fish Prior to Entry into Nature.

    abstract::Salmonids show a high degree of phenotypic plasticity that can differ among genotypes, and this variation is one of the major factors contributing to uncertainty in extrapolating laboratory-based risk assessment data to nature. Many studies have examined the relative growth and survival of transgenic and non-transgeni...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-35826-1

    authors: Vandersteen WE,Leggatt R,Sundström LF,Devlin RH

    更新日期:2019-01-23 00:00:00

  • YebC, a putative transcriptional factor involved in the regulation of the proteolytic system of Lactobacillus.

    abstract::The proteolytic system of Lactobacillus plays an essential role in bacterial growth, contributes to the flavor development of fermented products, and can release bioactive health-beneficial peptides during milk fermentation. In this work, a genomic analysis of all genes involved in the proteolytic system of L. delbrue...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-09124-1

    authors: Brown L,Villegas JM,Elean M,Fadda S,Mozzi F,Saavedra L,Hebert EM

    更新日期:2017-08-17 00:00:00

  • Effects of polarization mode dispersion on polarization-entangled photons generated via broadband pumped spontaneous parametric down-conversion.

    abstract::An inexpensive and compact frequency multi-mode diode laser enables a compact two-photon polarization entanglement source via the continuous wave broadband pumped spontaneous parametric down-conversion (SPDC) process. Entanglement degradation caused by polarization mode dispersion (PMD) is one of the critical issues i...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep25846

    authors: Lim HT,Hong KH,Kim YH

    更新日期:2016-05-13 00:00:00

  • FGF-7 Dictates Osteocyte Cell Processes Through Beta-Catenin Transduction.

    abstract::It is well recognized that osteocytes communicate with each other via gap junctions and that connxin43 (Cx43) shows its great potential in gap junction for the contribution enabling transmission of small molecules and operating in an autocrine/a paracrine manner. Fibroblast growth factors (FGFs) play significant roles...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-33247-8

    authors: Liu XY,Li X,Bai MR,Chen X,Wang CL,Xie J,Ye L

    更新日期:2018-10-04 00:00:00

  • Geometry-dependent band shift and dielectric modification of nanoporous Si nanowires.

    abstract::In order to obtain a detailed understanding of the modulation of electronic properties in nanoporous Si (np-Si) nanowires with containing ordered, nanometer-sized cylindrical pores, we propose a theoretical method to clarify the band shift and associated with the dielectric modification determined by the geometrical p...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-14647-8

    authors: Yu WB,Ouyang G

    更新日期:2017-10-31 00:00:00

  • Author Correction: Handrail support interference in cardiac autonomic modulation adjustments in young adults during maximal exercise testing.

    abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

    journal_title:Scientific reports

    pub_type: 已发布勘误

    doi:10.1038/s41598-020-75176-5

    authors: de Oliveira GL,Marques AH,da Fonseca VF,Pozzolo BA,Panacioni F,Santos TC,Guedes AA,Mocelin AG,Bertin RL,Ulbrich AZ

    更新日期:2020-10-28 00:00:00

  • Evolution and diversity of transposable elements in fish genomes.

    abstract::Transposable elements (TEs) are genomic sequences that can move, multiply, and often form sizable fractions of vertebrate genomes. Fish belong to a unique group of vertebrates, since their karyotypes and genome sizes are more diverse and complex, with probably higher diversity and evolution specificity of TE. To inves...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-51888-1

    authors: Shao F,Han M,Peng Z

    更新日期:2019-10-28 00:00:00

  • Potassium depletion induces cellular conversion in the outer medullary collecting duct altering Notch signaling pathway.

    abstract::Potassium depletion affects AQP2 expression and the cellular composition of the kidney collecting duct. This, in turn, contributes to the development of a secondary form of nephrogenic diabetes insipidus and hypokalemic nephropathy. Here we show that after 14 days of potassium depletion, the cellular fraction of A-typ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-61882-7

    authors: Iervolino A,Prosperi F,De La Motte LR,Petrillo F,Spagnuolo M,D'Acierno M,Siccardi S,Perna AF,Christensen BM,Frische S,Capasso G,Trepiccione F

    更新日期:2020-03-31 00:00:00

  • A quick and sensitive diagnostic tool for detection of Maize streak virus.

    abstract::Maize streak virus disease (MSVD), caused by Maize streak virus (MSV; genus Mastrevirus), is one of the most severe and widespread viral diseases that adversely reduces maize yield and threatens food security in Africa. An effective control and management of MSVD requires robust and sensitive diagnostic tests capable ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-76612-2

    authors: Tembo M,Adediji AO,Bouvaine S,Chikoti PC,Seal SE,Silva G

    更新日期:2020-11-12 00:00:00

  • Low Complexity Compression and Speed Enhancement for Optical Scanning Holography.

    abstract::In this paper we report a low complexity compression method that is suitable for compact optical scanning holography (OSH) systems with different optical settings. Our proposed method can be divided into 2 major parts. First, an automatic decision maker is applied to select the rows of holographic pixels to be scanned...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep34724

    authors: Tsang PW,Poon TC,Liu JP,Kim T,Kim YS

    更新日期:2016-10-06 00:00:00

  • Increasing the distance between two monomers of topoisomerase IIβ under the action of antitumor agent 4β-sulfur-(benzimidazole) 4'-demethylepipodophyllotoxin.

    abstract::Topoisomerases II (Top2s) are a group of essential enzymes involved in replication, transcription, chromosome condensation, and segregation via altering DNA topology. The mechanism of the Top2s poisons such as etoposide (VP-16) was reported as stabilizing the Top2-DNA complex and engendering permanent DNA breakage. As...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-33366-2

    authors: Sun LY,Zhu LW,Tang YJ

    更新日期:2018-10-08 00:00:00

  • Soluble OX40L and JAG1 Induce Selective Proliferation of Functional Regulatory T-Cells Independent of canonical TCR signaling.

    abstract::Regulatory T-cells (Tregs) play a pivotal role in maintaining peripheral tolerance. Increasing Treg numbers/functions has been shown to ameliorate autoimmune diseases. However, common Treg expansion approaches use T-Cell Receptor (TCR)-mediated stimulation which also causes proliferation of effector T-cells (Teff). To...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep39751

    authors: Kumar P,Alharshawi K,Bhattacharya P,Marinelarena A,Haddad C,Sun Z,Chiba S,Epstein AL,Prabhakar BS

    更新日期:2017-01-03 00:00:00

  • A randomized feasibility study of the effect of ascorbic acid on post-angioplasty restenosis of hemodialysis vascular access (NCT03524846).

    abstract::Restenosis remains a significant problem after angioplasty of hemodialysis vascular access. Both experimental and clinical studies have shown a protective effect of antioxidants against post-angioplasty restenosis. A prospective, randomized, feasibility study was conducted to investigate the effect of ascorbic acid to...

    journal_title:Scientific reports

    pub_type: 杂志文章,随机对照试验

    doi:10.1038/s41598-019-47583-w

    authors: Yang CW,Wu CC,Luo CM,Chuang SY,Chen CH,Shen YF,Tarng DC

    更新日期:2019-07-31 00:00:00

  • Ultrafast synaptic events in a chalcogenide memristor.

    abstract::Compact and power-efficient plastic electronic synapses are of fundamental importance to overcoming the bottlenecks of developing a neuromorphic chip. Memristor is a strong contender among the various electronic synapses in existence today. However, the speeds of synaptic events are relatively slow in most attempts at...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep01619

    authors: Li Y,Zhong Y,Xu L,Zhang J,Xu X,Sun H,Miao X

    更新日期:2013-01-01 00:00:00

  • Green gravel: a novel restoration tool to combat kelp forest decline.

    abstract::Kelp forests are in decline globally and large-scale intervention could be required to halt the loss of these valuable ecosystems. To date kelp forest restoration has had limited success and been expensive and unable to address the increasing scale of ecosystem deterioration. Here we developed and tested a new approac...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-60553-x

    authors: Fredriksen S,Filbee-Dexter K,Norderhaug KM,Steen H,Bodvin T,Coleman MA,Moy F,Wernberg T

    更新日期:2020-03-04 00:00:00

  • Ultra-sensitive detection of kanamycin for food safety using a reduced graphene oxide-based fluorescent aptasensor.

    abstract::Overuse of antibiotics has caused serious problems, such as appearance of super bacteria, whose accumulation in the human body through the food chain is a concern. Kanamycin is a common antibiotic used to treat diverse infections; however, residual kanamycin can cause many side effects in humans. Thus, development of ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep40305

    authors: Ha NR,Jung IP,La IJ,Jung HS,Yoon MY

    更新日期:2017-01-05 00:00:00

  • Interactions between two QTLs for time to anthesis on spike development and fertility in wheat.

    abstract::Earliness per se (Eps) genes are reported to be important in fine-tuning flowering time in wheat independently of photoperiod (Ppd) and vernalisation (Vrn). Unlike Ppd and Vrn genes, Eps have relatively small effects and their physiological effect along with chromosomal position are not well defined. We evaluated eigh...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-021-81857-6

    authors: Basavaraddi PA,Savin R,Wingen LU,Bencivenga S,Przewieslik-Allen AM,Griffiths S,Slafer GA

    更新日期:2021-01-28 00:00:00

  • Core-shell structured titanium-nitrogen alloys with high strength, high thermal stability and good plasticity.

    abstract::Multifunctional materials with more than two good properties are widely required in modern industries. However, some properties are often trade-off with each other by single microstructural designation. For example, nanostructured materials have high strength, but low ductility and thermal stability. Here by means of ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep40039

    authors: Zhang YS,Zhao YH,Zhang W,Lu JW,Hu JJ,Huo WT,Zhang PX

    更新日期:2017-01-06 00:00:00

  • Large epidemic thresholds emerge in heterogeneous networks of heterogeneous nodes.

    abstract::One of the famous results of network science states that networks with heterogeneous connectivity are more susceptible to epidemic spreading than their more homogeneous counterparts. In particular, in networks of identical nodes it has been shown that network heterogeneity, i.e. a broad degree distribution, can lower ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep13122

    authors: Yang H,Tang M,Gross T

    更新日期:2015-08-21 00:00:00