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A quick and sensitive diagnostic tool for detection of Maize streak virus.

Abstract：

:Maize streak virus disease (MSVD), caused by Maize streak virus (MSV; genus Mastrevirus), is one of the most severe and widespread viral diseases that adversely reduces maize yield and threatens food security in Africa. An effective control and management of MSVD requires robust and sensitive diagnostic tests capable of rapid detection of MSV. In this study, a loop-mediated isothermal amplification (LAMP) assay was designed for the specific detection of MSV. This test has shown to be highly specific and reproducible and able to detect MSV in as little as 10 fg/µl of purified genomic DNA obtained from a MSV-infected maize plant, a sensitivity 105 times higher to that obtained with polymerase chain reaction (PCR) in current general use. The high degree of sequence identity between Zambian and other African MSV isolates indicate that this LAMP assay can be used for detecting MSV in maize samples from any region in Africa. Furthermore, this assay can be adopted in minimally equipped laboratories and with potential use in plant clinic laboratories across Africa strengthening diagnostic capacity in countries dealing with MSD.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Tembo M,Adediji AO,Bouvaine S,Chikoti PC,Seal SE,Silva G

doi

10.1038/s41598-020-76612-2

subject

Has Abstract

pub_date

2020-11-12 00:00:00

pages

19633

issue

1

issn

2045-2322

pii

10.1038/s41598-020-76612-2

journal_volume

10

pub_type

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