Classical experiments in whole-body metabolism: open-circuit respirometry-diluted flow chamber, hood, or facemask systems.

Abstract:

:For over two centuries, scientists have measured gas exchange in animals and humans and linked this to energy expenditure of the body. The aim of this review is to provide a comprehensive overview of open-circuit diluted flow indirect calorimetry and to help researchers to make the optimal choice for a certain system and its application. A historical perspective shows that 'open circuit diluted flow' is a technique first used in the 19th century and applicable today for room calorimeters, ventilated hood systems, and facemasks. Room calorimeters are a classic example of an open-circuit diluted flow system. The broadly applied ventilated hood calorimeters follow the same principle and can be classified as a derivative of these room calorimeters. The basic principle is that the subject breathes freely in a passing airflow that is fully captured and analyzed. Oxygen and CO2 concentrations are measured in inlet ambient air and captured outlet air. The airflow, which is adapted depending on the application (e.g., rest versus exercise), is measured. For a room indirect calorimeter, the dilution in the large room volume is also taken into account, and this is the most complex application of this type of calorimeter. Validity of the systems can be tested by alcohol burns, gas infusions and by performing repeated measurements on subjects. Using the latter, the smallest CV (%) was found for repeated VO2max tests (1.2%) with an SD of approximately 1 kJ min-1. The smallest SD was found for sleeping metabolic rate (0.11 kJ min-1) with a CV (%) of 2.4%.

journal_name

Eur J Appl Physiol

authors

Schoffelen PFM,Plasqui G

doi

10.1007/s00421-017-3735-5

subject

Has Abstract

pub_date

2018-01-01 00:00:00

pages

33-49

issue

1

eissn

1439-6319

issn

1439-6327

pii

10.1007/s00421-017-3735-5

journal_volume

118

pub_type

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