Validation of a new calibration method for human muscle microdialysis at rest and during exercise.

Abstract:

:Microdialysis presents the unique possibility to measure metabolite concentrations in human interstitial fluid. During exercise, the recovery of these metabolites should be precisely monitored since it is known to increase greatly with muscle blood flow. The loss of ethanol, perfused at low concentration, can be accurately measured and reflects the changes in dialysis conditions. We evaluated whether using the relationship determined in resting metabolic conditions between the loss of ethanol, as reference substance, and the recovery for lactate or glucose would allow us to calculate precisely the concentration of these substances and their variations during exercise. Using the new catheter calibration method (slope method), the error of estimation of lactate and glucose in vitro was limited to -0.6 (5.8)% and -0.7 (6.2)%, respectively. In resting human muscle, the slope method proved to be as accurate as an established calibration technique ("no net flux method") to evaluate interstitial lactate concentration [1.82 (0.58) vs 1.83 (0.47) mM, respectively]. During dynamic knee-extension exercise or light neuromuscular electrical stimulation, the estimated interstitial lactate and glucose concentrations varied differently, but their time course changes remained consistent with their respective plasma values. We conclude that, after an initial calibration step, the slope method allows accurate measurement of interstitial muscle metabolites and it could be used to monitor rapid metabolic changes during exercise.

journal_name

Eur J Appl Physiol

authors

Desvigne N,Barthélémy JC,Bertholon F,Gay-Montchamp JP,Freyssenet D,Costes F

doi

10.1007/s00421-004-1099-0

keywords:

subject

Has Abstract

pub_date

2004-07-01 00:00:00

pages

312-20

issue

3

eissn

1439-6319

issn

1439-6327

journal_volume

92

pub_type

临床试验,杂志文章
  • Topical application of cream containing nonivamide and nicoboxil does not enhance the performance of experienced cyclists during a 4-km time-trial.

    abstract:PURPOSE:Topical application of nonivamide-nicoboxil cream to resting legs has been shown to enhance the level of oxygenated haemoglobin in leg muscles 15 min later. Here, we examined whether such application improves the performance of experienced cyclists in a subsequent 4-km time-trial. METHODS:Nine male cyclists [2...

    journal_title:European journal of applied physiology

    pub_type: 杂志文章

    doi:10.1007/s00421-016-3357-3

    authors: Zinner C,Holmberg HC,Sperlich B

    更新日期:2016-05-01 00:00:00

  • Creatine kinase MM TaqI and methylenetetrahydrofolate reductase C677T and A1298C gene polymorphisms influence exercise-induced C-reactive protein levels.

    abstract::Physical training induces beneficial adaptations, but exhausting exercise increases reactive oxygen species, which can cause muscular injuries with consequent inflammatory processes, implying jeopardized performance and possibly overtraining. Acute strenuous exercise almost certainly exceeds the benefits of physical a...

    journal_title:European journal of applied physiology

    pub_type: 杂志文章

    doi:10.1007/s00421-011-1967-3

    authors: Miranda-Vilela AL,Akimoto AK,Lordelo GS,Pereira LC,Grisolia CK,Klautau-Guimarães Mde N

    更新日期:2012-01-01 00:00:00

  • Cerebral oxygenation during hyperoxia-induced increase in exercise tolerance for untrained men.

    abstract::This study aimed to investigate the involvement of cerebral oxygenation in limitation of maximal exercise. We hypothesized that O2 supplementation improves physical performance in relation to its effect on cerebral oxygenation during exercise. Eight untrained men (age 27 ± 6 years; VO2 max 45 ± 8 ml min(-1) kg(-1)) pe...

    journal_title:European journal of applied physiology

    pub_type: 杂志文章,随机对照试验

    doi:10.1007/s00421-013-2637-4

    authors: Oussaidene K,Prieur F,Bougault V,Borel B,Matran R,Mucci P

    更新日期:2013-08-01 00:00:00

  • Effect of short-term creatine supplementation on markers of skeletal muscle damage after strenuous contractile activity.

    abstract::The protective effect of short-term creatine supplementation (CrS) upon markers of strenuous contractile activity-induced damage in human and rat skeletal muscles was investigated. Eight Ironman triathletes were randomized into the placebo (Pl; n = 4) and creatine-supplemented (CrS; n = 4) groups. Five days prior to t...

    journal_title:European journal of applied physiology

    pub_type: 杂志文章,随机对照试验

    doi:10.1007/s00421-009-1305-1

    authors: Bassit RA,Pinheiro CH,Vitzel KF,Sproesser AJ,Silveira LR,Curi R

    更新日期:2010-03-01 00:00:00

  • Exercise training improves vascular function in patients with Alzheimer's disease.

    abstract:PURPOSE:Vascular dysfunction has been demonstrated in patients with Alzheimer's disease (AD). Exercise is known to positively affect vascular function. Thus, the aim of our study was to investigate exercise-induced effects on vascular function in AD. METHODS:Thirty-nine patients with AD (79 ± 8 years) were recruited a...

    journal_title:European journal of applied physiology

    pub_type: 杂志文章

    doi:10.1007/s00421-020-04447-w

    authors: Pedrinolla A,Venturelli M,Fonte C,Tamburin S,Di Baldassarre A,Naro F,Varalta V,Giuriato G,Ghinassi B,Muti E,Smania N,Schena F

    更新日期:2020-10-01 00:00:00

  • Stability of daily home-based measures of postural control over an 8-week period in highly functioning older adults.

    abstract:PURPOSE:The focus of this study was to monitor daily objective measures of standing postural control over an 8-week period, recorded in a person's home, in a population of healthy older adults. Establishing natural patterns of variation in the day-to-day signal, occurring in the relative absence of functional decline o...

    journal_title:European journal of applied physiology

    pub_type: 杂志文章

    doi:10.1007/s00421-014-3034-3

    authors: McGrath D,Greene BR,Sheehan K,Walsh L,Kenny RA,Caulfield B

    更新日期:2015-02-01 00:00:00

  • Low frequency of the "plateau phenomenon" during maximal exercise in elite British athletes.

    abstract::A plateau in oxygen consumption (VO(2)) has long been considered the criterion for maximal effort during an incremental exercise test. But, surprisingly, the termination of a maximum exercise test often occurs in the absence of a VO(2) plateau. To explain this inconsistency, some have proposed that an oxygen limitatio...

    journal_title:European journal of applied physiology

    pub_type: 杂志文章

    doi:10.1007/s00421-003-0845-z

    authors: Doherty M,Nobbs L,Noakes TD

    更新日期:2003-08-01 00:00:00

  • Effects of vibration training on bone metabolism: results from a short-term bed rest study.

    abstract::The absence of mechanical loading leads to a prompt increase in bone resorption measured by bone resorption markers. There is high potential that vibration training can positively influence bone metabolism in immobilized subjects, reduce the increase in osteoclastic activity and increase bone formation processes. We i...

    journal_title:European journal of applied physiology

    pub_type: 杂志文章

    doi:10.1007/s00421-011-2137-3

    authors: Baecker N,Frings-Meuthen P,Heer M,Mester J,Liphardt AM

    更新日期:2012-05-01 00:00:00

  • Proteomic assessment of the acute phase of dystrophin deficiency in mdx mice.

    abstract::Duchenne muscular dystrophy (DMD) is caused by the absence of a functional dystrophin protein and is modeled by the mdx mouse. The mdx mouse suffers an early necrotic bout in the hind limb muscles lasting from approximately 4 to 7 weeks. The purpose of this investigation was to determine the extent to which dystrophin...

    journal_title:European journal of applied physiology

    pub_type: 杂志文章

    doi:10.1007/s00421-011-1906-3

    authors: Gardan-Salmon D,Dixon JM,Lonergan SM,Selsby JT

    更新日期:2011-11-01 00:00:00

  • The energetics of ultra-endurance running.

    abstract::Our objective was to determine the effects of long-lasting endurance events on the energy cost of running (C(r)), and the role of maximal oxygen uptake (VO(2max)), its fractional utilisation (F) and C(r) in determining the performance. Ten healthy runners (age range 26-59 years) participated in an ultra-endurance comp...

    journal_title:European journal of applied physiology

    pub_type: 杂志文章

    doi:10.1007/s00421-011-2120-z

    authors: Lazzer S,Salvadego D,Rejc E,Buglione A,Antonutto G,di Prampero PE

    更新日期:2012-05-01 00:00:00

  • Resistance training upregulates skeletal muscle Na+, K+-ATPase content, with elevations in both α1 and α2, but not β isoforms.

    abstract:PURPOSE:The Na+, K+-ATPase (NKA) is important in regulating trans-membrane ion gradients, cellular excitability and muscle function. We investigated the effects of resistance training in healthy young adults on the adaptability of NKA content and of the specific α and β isoforms in human skeletal muscle. METHODS:Twent...

    journal_title:European journal of applied physiology

    pub_type: 杂志文章

    doi:10.1007/s00421-020-04408-3

    authors: Altarawneh MM,Petersen AC,Farr T,Garnham A,Broatch JR,Halson S,Bishop DJ,McKenna MJ

    更新日期:2020-08-01 00:00:00

  • Acute muscle and joint mechanical responses following a high-intensity stretching protocol.

    abstract:PURPOSE:A previous study observed a joint passive torque increase above baseline ~30 min after a high-intensity stretching. This study examined the effect of a high-intensity stretching on ankle dorsiflexion passive torque, medial gastrocnemius (MG) shear modulus, and plantar flexors maximal voluntary isometric force (...

    journal_title:European journal of applied physiology

    pub_type: 杂志文章,随机对照试验

    doi:10.1007/s00421-016-3410-2

    authors: Freitas SR,Andrade RJ,Nordez A,Mendes B,Mil-Homens P

    更新日期:2016-08-01 00:00:00

  • The influences of muscle fibre proportions and areas upon EMG during maximal dynamic knee extensions.

    abstract::This study is an investigation of the relationship between muscle morphology and surface electromyographic (EMG) parameters [mean frequency of the power spectrum (MNF), signal amplitude (root mean square, RMS) and the signal amplitude ratio (SAR; i.e. the ratio between the RMS level during the passive part of the cont...

    journal_title:European journal of applied physiology

    pub_type: 杂志文章

    doi:10.1007/PL00013792

    authors: Gerdle B,Karlsson S,Crenshaw AG,Elert J,Fridén J

    更新日期:2000-01-01 00:00:00

  • Concurrent improvements in cardiorespiratory and muscle fitness in response to total body recumbent stepping in humans.

    abstract::A group of 26 sedentary adults [mean age 48.4 (SD 6.4) years] were allocated randomly into either a non-exercising control group (CON, n =9) or an exercise group (EX, n = 17) that trained 3 days a week for 12 weeks using a total body recumbent stepper (TBRS). Training intensity and duration progressed from 50% of hear...

    journal_title:European journal of applied physiology

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1007/s004210100435

    authors: Hass CJ,Garzarella L,de Hoyos DV,Connaughton DP,Pollock ML

    更新日期:2001-07-01 00:00:00

  • Changes in resting mitogen-activated protein kinases following resistance exercise overreaching and overtraining.

    abstract:PURPOSE:Many physiological maladaptations persist after overreaching and overtraining resistance exercise (RE). However, no studies have investigated changes in mitogen-activated protein kinases (MAPK) after overtraining in humans, despite their critical role regulating exercise-induced muscular adaptations. The purpos...

    journal_title:European journal of applied physiology

    pub_type: 杂志文章

    doi:10.1007/s00421-016-3492-x

    authors: Nicoll JX,Fry AC,Galpin AJ,Sterczala AJ,Thomason DB,Moore CA,Weiss LW,Chiu LZ

    更新日期:2016-12-01 00:00:00

  • Pain sensitivity is normalized after a repeated bout of eccentric exercise.

    abstract:PURPOSE:The purpose of this study was to investigate the effect of repeated bouts of eccentric exercise on the nociceptive withdrawal reflex (NWR) threshold, a measure of sensitivity in the spinal nociceptive system. METHODS:Sixteen healthy students (age 25.7 ± 0.6 years, BMI 24.8 ± 1 kg m(-2)) participated in this ra...

    journal_title:European journal of applied physiology

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1007/s00421-013-2701-0

    authors: Hosseinzadeh M,Andersen OK,Arendt-Nielsen L,Madeleine P

    更新日期:2013-10-01 00:00:00

  • Effect of prolonged exercise and pre-exercise dietary manipulation on hepatic triglycerides in trained men.

    abstract::The purpose of this study was to examine the effect of exercise and pre-exercise dietary manipulation on hepatic triglyceride concentration (HTGC). HTGC was measured by proton magnetic resonance spectroscopy ((1)H-MRS) before and after 90 min of moderate intensity cycling in six endurance trained males following 67 h ...

    journal_title:European journal of applied physiology

    pub_type: 杂志文章

    doi:10.1007/s00421-011-2158-y

    authors: Johnson NA,van Overbeek D,Chapman PG,Thompson MW,Sachinwalla T,George J

    更新日期:2012-05-01 00:00:00

  • Fatiguing handgrip exercise alters maximal force-generating capacity of plantar-flexors.

    abstract::Exercise-induced fatigue causes changes within the central nervous system that decrease force production capacity in fatigued muscles. The impact on unrelated, non-exercised muscle performance is still unclear. The primary aim of this study was to examine the impact of a bilateral forearm muscle contraction on the mot...

    journal_title:European journal of applied physiology

    pub_type: 杂志文章,随机对照试验

    doi:10.1007/s00421-012-2462-1

    authors: Kennedy A,Hug F,Sveistrup H,Guével A

    更新日期:2013-03-01 00:00:00

  • Quantitative assessment of nitric oxide in rat skeletal muscle and plasma after exercise.

    abstract::Nitric oxide (NO), a short-lived vasoactive substance that has multiple physiological functions, is also involved in skeletal muscle physiology. This work examines the levels of nitrate (the metabolic end-product of NO) in muscle and plasma after different exercise protocols: namely acute, eccentric, cardiac stress an...

    journal_title:European journal of applied physiology

    pub_type: 杂志文章

    doi:10.1007/s00421-002-0693-2

    authors: Perez AC,de Oliveira CC,Prieto JG,Ferrando A,Vila L,Alvarez AI

    更新日期:2002-11-01 00:00:00

  • The impact of sleeping with reduced glycogen stores on immunity and sleep in triathletes.

    abstract:PURPOSE:We investigated the effects of a 3-week dietary periodization on immunity and sleep in triathletes. METHODS:21 triathletes were divided into two groups with different nutritional guidelines during a 3-week endurance training program including nine twice a day sessions with lowered (SL group) or maintained (CON...

    journal_title:European journal of applied physiology

    pub_type: 杂志文章,随机对照试验

    doi:10.1007/s00421-016-3446-3

    authors: Louis J,Marquet LA,Tiollier E,Bermon S,Hausswirth C,Brisswalter J

    更新日期:2016-10-01 00:00:00

  • Comparison and reliability of two non-invasive acetylene uptake techniques for the measurement of cardiac output.

    abstract::Comparison and reliability of two non-invasive acetylene uptake techniques for the measurement of cardiac output. Thirteen trained male cyclists performed CO2 rebreathing (CO2RB) at intensities from rest to 200 W, and open-circuit acetylene uptake (OpCirc) and single-breath acetylene uptake (SB) at intensities from re...

    journal_title:European journal of applied physiology

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1007/s00421-005-1343-2

    authors: Dibski DW,Smith DJ,Jensen R,Norris SR,Ford GT

    更新日期:2005-08-01 00:00:00

  • Effects of neuromuscular fatigue on the electromechanical delay of the leg extensors and flexors in young and old men.

    abstract:PURPOSE:The purpose of this study was to investigate the effects of a fatigue-inducing bout of submaximal, intermittent isometric contractions on the electromechanical delay (EMD) of the leg extensors and flexors in young and old men. METHODS:Twenty young (mean ± SD: age = 25 ± 2.8 years) and sixteen old (age = 70.8 ±...

    journal_title:European journal of applied physiology

    pub_type: 杂志文章,随机对照试验

    doi:10.1007/s00421-013-2675-y

    authors: Conchola EC,Thompson BJ,Smith DB

    更新日期:2013-09-01 00:00:00

  • RPE-lactate dissociation during extended cycling.

    abstract::This study examined the association of blood lactate concentration [La] and heart rate (HR) with ratings of perceived exertion (RPE) during 60 min of steady workload cycling. Physically active college-aged subjects (n = 14) completed an exhaustive cycling test to determine VO(2) (peak) and lactate threshold (2.5 mmol ...

    journal_title:European journal of applied physiology

    pub_type: 临床试验,杂志文章

    doi:10.1007/s00421-004-1311-2

    authors: Green JM,McLester JR,Crews TR,Wickwire PJ,Pritchett RC,Redden A

    更新日期:2005-05-01 00:00:00

  • Impact of repeated bouts of eccentric exercise on myogenic gene expression.

    abstract::Evidence indicates that repeated-bouts of eccentric exercise (EE) do not exacerbate the extent of muscle damage indices, as compared to a single-bout. We hypothesized that molecular adaptations, under repeated-bouts of EE, would include suppression of muscle repair inhibitory factors such as myostatin and up-regulatio...

    journal_title:European journal of applied physiology

    pub_type: 杂志文章

    doi:10.1007/s00421-007-0510-z

    authors: Costa A,Dalloul H,Hegyesi H,Apor P,Csende Z,Racz L,Vaczi M,Tihanyi J

    更新日期:2007-11-01 00:00:00

  • Validity and reliability of three commercially available breath-by-breath respiratory systems.

    abstract::Information concerning the validity and reliability of commercial on-line gas analysis systems is limited. The aim of this study was to provide a comparison of the validity and reliability of three on-line systems (Oxycon Alpha, Oxycon Pro and Pulmolab EX670) with that of Douglas bags. Two separate studies were conduc...

    journal_title:European journal of applied physiology

    pub_type: 杂志文章

    doi:10.1007/s00421-001-0572-2

    authors: Carter J,Jeukendrup AE

    更新日期:2002-03-01 00:00:00

  • Alternative strategies for exercise critical power estimation in patients with COPD.

    abstract::Exercise critical power (CP) has been shown to represent the highest sustainable work rate (WR) in patients with chronic obstructive pulmonary disease (COPD). Parameter estimation, however, depends on 4 high-intensity tests performed, on different days, to the limit of tolerance (T(lim)). In order to establish a milde...

    journal_title:European journal of applied physiology

    pub_type: 杂志文章

    doi:10.1007/s00421-005-0064-x

    authors: Malaguti C,Nery LE,Dal Corso S,De Fuccio MB,Lerario MC,Cendon S,Neder JA

    更新日期:2006-01-01 00:00:00

  • Multilevel allometric modelling of maximum cardiac output, maximum arteriovenous oxygen difference, and peak oxygen uptake in 11-13-year-olds.

    abstract:PURPOSES:To investigate longitudinally (1) the contribution of morphological covariates to explaining the development of maximum cardiac output ([Formula: see text] max) and maximum arteriovenous oxygen difference (a-vO2 diff max), (2) sex differences in [Formula: see text] max and a-vO2 diff max once age, maturity sta...

    journal_title:European journal of applied physiology

    pub_type: 杂志文章

    doi:10.1007/s00421-020-04300-0

    authors: Armstrong N,Welsman J

    更新日期:2020-02-01 00:00:00

  • The effect of acute exercise in hypoxia on flow-mediated vasodilation.

    abstract::The purpose of this study was to clarify the effect of acute exercise in hypoxia on flow-mediated vasodilation (FMD). Eight males participated in this study. Two maximal exercise tests were performed using arm cycle ergometry to estimate peak oxygen uptake [Formula: see text] while breathing normoxic [inspired O(2) fr...

    journal_title:European journal of applied physiology

    pub_type: 杂志文章,随机对照试验

    doi:10.1007/s00421-012-2442-5

    authors: Katayama K,Fujita O,Iemitsu M,Kawano H,Iwamoto E,Saito M,Ishida K

    更新日期:2013-02-01 00:00:00

  • Altered tension cost in (TG(mREN-2)27) rats overexpressing the mouse renin gene.

    abstract::The present study aimed to characterize cardiac hypertrophy induced by activation of the renin-angiotensin system in terms of functional alterations on the level of the contractile proteins, employing transgenic rats harboring the mouse renin gene (TGR(mREN2)27). Ca2+-dependent tension and myosin ATPase activity were ...

    journal_title:European journal of applied physiology

    pub_type: 杂志文章

    doi:10.1007/s00421-006-0323-5

    authors: Zobel C,Zavidou-Saroti P,Bölck B,Brixius K,Reuter H,Frank K,Diedrichs H,Müller-Ehmsen J,Bloch W,Schwinger RH

    更新日期:2007-01-01 00:00:00

  • The order effect of combined endurance and strength loadings on force and hormone responses: effects of prolonged training.

    abstract:PURPOSE:To examine acute responses and recovery of force and serum hormones to combined endurance and strength loadings utilizing different orders of exercises before and after training. METHODS:Physically active men were matched to an order sequence of endurance followed by strength (E + S, n = 12) or strength follow...

    journal_title:European journal of applied physiology

    pub_type: 杂志文章

    doi:10.1007/s00421-013-2813-6

    authors: Schumann M,Walker S,Izquierdo M,Newton RU,Kraemer WJ,Häkkinen K

    更新日期:2014-04-01 00:00:00